MD01G1161000.v1.1

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012162
Physical Location & Seq
Reverse (-)
26640259 .. 26643214
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1161000.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGTTTCGACTGATAATGGAAACCACAAGGAGGAAATTGAAGAATCGAATGGTAGTGAAATGAAGGGTCAAACCCCCTTTGAGGTTTCAGCATCCCGGAAGACTTTTTTAACCAATGAGAAGTCTCCGAAAAGGTTCCCTCCTGATGCTCCTCTTAATAGAGTTAGTTTCTTCAAATTTGGTTCTGCATCTGCCAGGTTCAAGCGGTTGGCTGAGGAGAGAGATGAAATGTCACGATCTGTGGCTTCAAGCGGCCATGGCTTTAAAGAACGAATCCATGGGGTTTTTTCTAAGAAAATTGATTGGGTTTCACTCGTGAAAATGGGCAAAGAATGGATTAGAAACCCAATGAACATGGCTCTTTTTGCGTGGATCGTCTGTGTTGCTGTTTCGGGTGCAATTTTGTTTCTTGTCATGACTGGAATGTTGAACAAGGCAATACCCAAGAAGTCCGAGAGAGATGCATGGTTTGAAGTCAATAATCAAATTCTCAATGCACTTTTTACTCTTTTGTGTTTGTACAACCATCCGAAGCGATTCTACCACCTTGTACTTCTTTGTAGATGGAAACCAGAAGACATTTCCAGACTTCGAAAGGTTTACTGTAAAAATGGTACTTATAAGCCCCACGAATGGGCCCACATGATGGTTGTTGTTGCCCTGCTTCATCTGAATTGCTTTGGTCAGTATGCACTTTGTGGTTTAAACTTGGGTTACAGAAGAGCTCAACGACCACCGATAGGAGTTGCAATATGTATATCTTTTGCTATTGGTGCACCTGCATTTGCTGGTATATACAACATTGTCAGCCCCCTTGGGAAGGATTATGATTCAGAAGTGGATGTGGAAGCACATGTTCCAATCGGCACTGGTGAAGGAGCAGAAAGATCAAGGTCAAAAACATTTGAGAGAAAATATTCTTTTGCATTTAGGACTGACCAACGAGTTGTTGAGAGTAAACCACAATGGAGCGGAGGAGTACTTGACATTTGGGATGATATTTCTATAGCGTATGTTTCACTCTTTTGTAGTTTTTGTGTGTTCGGGTGGAATATGGAGAGGCTTGGATTCGGGAACATGTATGTTCATATTATGACATTTATTCTGTTTTGCATGGCTCCGTTCTGGATTTTTAACTTGGCTGCTGTTAATATCGACAATGAAACTGTTCGGGGGGCTCTTGGAGTTACTGGCCTAGTTCTATGTCTATTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTCAATTTGCCTTCTTATAACTTCTGCTTTGGTAAACCAGCACTCTCTGATTGCACGTTGTGGCTTTGCTGTTGTTGGTGCTCTCTTGCTCAGGAAGTGAGGACCGGGAATTCCTATGACGTTGTGGAAGAAAAGTTTTGTAGAAAACAACAGGAAAGTGAAGGCCTTCCACTAATTTCACCTTTGCCTCGTGAAGATGGATCGCTTGAGTTTAGATCTGGTCCGAATTCTCCTCTGGGGAACTCCTTAAGCCCAACCAAGAATGGCATCGGTGGTTCTCCGAGTCCAAGCGGACTTCCAAAGGGATTTCATAGTCCAGGTAGGCCACTTTCTGCAGTGAAAGAAGAGTCCCATGTAAGAGGTAAGGATGAAACTATGACCCCACCTGCACCTTCATTGATACAAAGGGAAGAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

559

Amino Acids

62.71

Weight (kDa)

8.88

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 111 - 189 5.5e-30 Protein of unknown function (DUF2985)
PLAC8 PF04749 323 - 453 1.3e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 621, 1275
AarI CACCTGC 2 cut(s) 787, 1651
Acc36I ACCTGC 2 cut(s) 787, 1651
AccB7I CCANNNNNTGG 1 cut(s) 646
AccBSI CCGCTC 1 cut(s) 972
AciI CCGC 4 cut(s) 205, 252, 972, 1548
AclWI GGATC 4 cut(s) 380, 1234, 1247, 1465
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 4 cut(s) 176, 486, 1366, 1483
AdeI CACNNNGTG 2 cut(s) 698, 1317
AfaI GTAC 4 cut(s) 521, 552, 616, 981
AfiI CCNNNNNNNGG 7 cut(s) 31, 82, 633, 634, 646, 740, 820
AflII CTTAAG 1 cut(s) 1504
AflIII ACRYGT 2 cut(s) 853, 1077
AgsI TTSAA 7 cut(s) 41, 175, 202, 249, 430, 473, 1261
AjnI CCWGG 2 cut(s) 194, 1573
AluBI AGCT 1 cut(s) 725
AluI AGCT 1 cut(s) 725
Alw21I GWGCWC 3 cut(s) 727, 778, 1340
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 774
AlwI GGATC 4 cut(s) 380, 1234, 1247, 1465
AoxI GGCC 5 cut(s) 253, 636, 1192, 1420, 1580
ApaI GGGCCC 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 774
ApeKI GCWGC 1 cut(s) 1142
ApoI RAATTY 4 cut(s) 176, 486, 1366, 1483
Asp700I GAANNNNTTC 5 cut(s) 134, 536, 916, 1167, 1422
AspS9I GGNCC 4 cut(s) 636, 637, 1359, 1478
AsuC2I CCSGG 2 cut(s) 97, 1363
AsuHPI GGTGA 2 cut(s) 884, 1428
AsuII TTCGAA 1 cut(s) 592
AvaII GGWCC 2 cut(s) 1359, 1478
BaeGI GKGCMC 2 cut(s) 640, 778
BamHI GGATCC 1 cut(s) 1239
BanII GRGCYC 3 cut(s) 640, 727, 1180
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BauI CACGAG 2 cut(s) 314, 1446
BbsI GAAGAC 2 cut(s) 107, 582
Bbv12I GWGCWC 3 cut(s) 727, 778, 1340
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 1129
BccI CCATC 4 cut(s) 534, 558, 640, 1448
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BciT130I CCWGG 2 cut(s) 196, 1575
BcnI CCSGG 2 cut(s) 97, 1363
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 1196
BfmI CTRYAG 2 cut(s) 1005, 1590
BfrI CTTAAG 1 cut(s) 1504
BfuAI ACCTGC 2 cut(s) 787, 1651
BglII AGATCT 1 cut(s) 1472
BisI GCNGC 2 cut(s) 253, 1143
BlsI GCNGC 2 cut(s) 254, 1144
BmcAI AGTACT 1 cut(s) 981
Bme1390I CCNGG 4 cut(s) 97, 196, 1363, 1575
Bme18I GGWCC 2 cut(s) 1359, 1478
BmgT120I GGNCC 4 cut(s) 636, 637, 1359, 1478
BmiI GGNNCC 4 cut(s) 137, 638, 1119, 1241
BmrFI CCNGG 4 cut(s) 97, 196, 1363, 1575
BmsI GCATC 5 cut(s) 101, 136, 197, 451, 1533
BpiI GAAGAC 2 cut(s) 107, 582
Bpu10I CCTNAGC 2 cut(s) 213, 1347
Bpu14I TTCGAA 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 1484
BpuMI CCSGG 2 cut(s) 97, 1363
BsaJI CCNNGG 3 cut(s) 256, 277, 814
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 82, 633, 634, 646, 740, 820
Bse1I ACTGG 3 cut(s) 424, 874, 1195
BseBI CCWGG 2 cut(s) 196, 1575
BseDI CCNNGG 3 cut(s) 256, 277, 814
BseGI GGATG 5 cut(s) 92, 526, 847, 1000, 1630
BseLI CCNNNNNNNGG 7 cut(s) 31, 82, 633, 634, 646, 740, 820
BseMII CTCAG 2 cut(s) 204, 1361
BseNI ACTGG 3 cut(s) 424, 874, 1195
BseRI GAGGAG 4 cut(s) 141, 230, 990, 1479
BseSI GKGCMC 2 cut(s) 640, 778
BseXI GCAGC 1 cut(s) 1129
BsgI GTGCAG 1 cut(s) 1629
BshFI GGCC 5 cut(s) 255, 638, 1194, 1422, 1582
BsiHKAI GWGCWC 3 cut(s) 727, 778, 1340
BsiSI CCGG 2 cut(s) 97, 1362
BslFI GGGAC 1 cut(s) 1591
BslI CCNNNNNNNGG 7 cut(s) 31, 82, 633, 634, 646, 740, 820
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 1591
BsnI GGCC 5 cut(s) 255, 638, 1194, 1422, 1582
Bsp119I TTCGAA 1 cut(s) 592
Bsp120I GGGCCC 1 cut(s) 636
Bsp1286I GDGCHC 5 cut(s) 640, 727, 778, 1180, 1340
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
Bsp19I CCATGG 2 cut(s) 256, 277
BspACI CCGC 4 cut(s) 205, 252, 972, 1548
BspANI GGCC 5 cut(s) 255, 638, 1194, 1422, 1582
BspCNI CTCAG 2 cut(s) 205, 1360
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 4 cut(s) 137, 638, 1119, 1241
BspMAI CTGCAG 1 cut(s) 1594
BspMI ACCTGC 2 cut(s) 787, 1651
BspPI GGATC 4 cut(s) 380, 1234, 1247, 1465
BspQI GCTCTTC 1 cut(s) 715
BspT104I TTCGAA 1 cut(s) 592
BspTI CTTAAG 1 cut(s) 1504
BsrBI CCGCTC 1 cut(s) 972
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 3 cut(s) 424, 874, 1195
BssECI CCNNGG 3 cut(s) 256, 277, 814
BssMI GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
BssSI CACGAG 2 cut(s) 314, 1446
BssT1I CCWWGG 3 cut(s) 256, 277, 814
Bst2BI CACGAG 2 cut(s) 314, 1446
Bst2UI CCWGG 2 cut(s) 196, 1575
Bst4CI ACNGT 2 cut(s) 605, 1168
Bst6I CTCTTC 2 cut(s) 715, 1596
BstAFI CTTAAG 1 cut(s) 1504
BstAUI TGTACA 1 cut(s) 519
BstBI TTCGAA 1 cut(s) 592
BstDEI CTNAG 3 cut(s) 213, 291, 1347
BstDSI CCRYGG 2 cut(s) 256, 277
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 5 cut(s) 92, 526, 847, 1000, 1630
BstKTI GATC 6 cut(s) 239, 375, 890, 1242, 1460, 1475
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
BstMWI GCNNNNNNNGC 3 cut(s) 258, 365, 773
BstNI CCWGG 2 cut(s) 196, 1575
BstNSI RCATGY 2 cut(s) 857, 1081
BstSCI CCNGG 4 cut(s) 95, 194, 1361, 1573
BstSFI CTRYAG 2 cut(s) 1005, 1590
BstSLI GKGCMC 2 cut(s) 640, 778
BstV1I GCAGC 1 cut(s) 1129
BstV2I GAAGAC 2 cut(s) 107, 582
BstX2I RGATCY 2 cut(s) 1239, 1472
BstYI RGATCY 2 cut(s) 1239, 1472
BsuRI GGCC 5 cut(s) 255, 638, 1194, 1422, 1582
BtgI CCRYGG 2 cut(s) 256, 277
BtsCI GGATG 5 cut(s) 92, 526, 847, 1000, 1630
BtsI GCAGTG 1 cut(s) 1599
BtsIMutI CAGTG 2 cut(s) 867, 1599
BveI ACCTGC 2 cut(s) 787, 1651
CciI TCATGA 1 cut(s) 414
Cfr13I GGNCC 4 cut(s) 636, 637, 1359, 1478
Csp6I GTAC 4 cut(s) 520, 551, 615, 980
CviQI GTAC 4 cut(s) 520, 551, 615, 980
DdeI CTNAG 3 cut(s) 213, 291, 1347
DpnI GATC 6 cut(s) 238, 374, 889, 1241, 1459, 1474
DpnII GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
DraI TTTAAA 2 cut(s) 265, 705
DraIII CACNNNGTG 2 cut(s) 698, 1317
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 715, 1596
EarI CTCTTC 2 cut(s) 715, 1596
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 3 cut(s) 256, 277, 814
Eco147I AGGCCT 1 cut(s) 1422
Eco24I GRGCYC 3 cut(s) 640, 727, 1180
Eco47I GGWCC 2 cut(s) 1359, 1478
Eco53kI GAGCTC 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 725
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoRI GAATTC 2 cut(s) 1366, 1483
EcoRII CCWGG 2 cut(s) 194, 1573
EcoT14I CCWWGG 3 cut(s) 256, 277, 814
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 3 cut(s) 640, 727, 1180
ErhI CCWWGG 3 cut(s) 256, 277, 814
FaqI GGGAC 1 cut(s) 1591
Fnu4HI GCNGC 2 cut(s) 253, 1143
FokI GGATG 5 cut(s) 79, 513, 854, 1007, 1637
FriOI GRGCYC 3 cut(s) 640, 727, 1180
Fsp4HI GCNGC 2 cut(s) 253, 1143
FspBI CTAG 1 cut(s) 1196
GluI GCNGC 2 cut(s) 253, 1143
HaeIII GGCC 5 cut(s) 255, 638, 1194, 1422, 1582
HapII CCGG 2 cut(s) 97, 1362
HinfI GANTC 8 cut(s) 44, 273, 537, 830, 1068, 1257, 1540, 1604
HpaII CCGG 2 cut(s) 97, 1362
HphI GGTGA 2 cut(s) 884, 1428
Hpy166II GTNNAC 4 cut(s) 601, 776, 959, 1292
Hpy188I TCNGA 8 cut(s) 129, 454, 531, 672, 835, 1306, 1482, 1539
Hpy188III TCNNGA 9 cut(s) 143, 234, 316, 415, 585, 1072, 1126, 1349, 1448
Hpy8I GTNNAC 4 cut(s) 601, 776, 959, 1292
HpyAV CCTTC 7 cut(s) 58, 814, 869, 1278, 1412, 1433, 1659
HpyCH4III ACNGT 2 cut(s) 605, 1168
HpyCH4IV ACGT 2 cut(s) 1313, 1377
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 365, 773
HpyF3I CTNAG 3 cut(s) 213, 291, 1347
HpySE526I ACGT 2 cut(s) 1313, 1377
Kzo9I GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
LguI GCTCTTC 1 cut(s) 715
LmnI GCTCC 4 cut(s) 154, 878, 969, 1123
Lsp1109I GCAGC 1 cut(s) 1129
LweI GCATC 5 cut(s) 101, 136, 197, 451, 1533
MaeI CTAG 1 cut(s) 1196
MaeII ACGT 2 cut(s) 1313, 1377
MaeIII GTNAC 3 cut(s) 231, 713, 1186
MalI GATC 6 cut(s) 238, 374, 889, 1241, 1459, 1474
MbiI CCGCTC 1 cut(s) 972
MboI GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
MboII GAAGA 8 cut(s) 53, 112, 163, 587, 732, 1397, 1463, 1613
MflI RGATCY 2 cut(s) 1239, 1472
MhlI GDGCHC 5 cut(s) 640, 727, 778, 1180, 1340
MlyI GAGTC 2 cut(s) 1549, 1613
Mph1103I ATGCAT 1 cut(s) 466
MroXI GAANNNNTTC 5 cut(s) 134, 536, 916, 1167, 1422
MseI TTAA 7 cut(s) 110, 156, 264, 704, 1134, 1149, 1505
MspCI CTTAAG 1 cut(s) 1504
MspI CCGG 2 cut(s) 97, 1362
MspR9I CCNGG 4 cut(s) 97, 196, 1363, 1575
MssI GTTTAAAC 1 cut(s) 705
MvaI CCWGG 2 cut(s) 196, 1575
MwoI GCNNNNNNNGC 3 cut(s) 258, 365, 773
NciI CCSGG 2 cut(s) 97, 1363
NcoI CCATGG 2 cut(s) 256, 277
NdeII GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
NlaIV GGNNCC 4 cut(s) 137, 638, 1119, 1241
NmuCI GTSAC 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 2 cut(s) 857, 1081
NspV TTCGAA 1 cut(s) 592
PagI TCATGA 1 cut(s) 414
PaqCI CACCTGC 2 cut(s) 787, 1651
PceI AGGCCT 1 cut(s) 1422
PciI ACATGT 2 cut(s) 853, 1077
PciSI GCTCTTC 1 cut(s) 715
PdmI GAANNNNTTC 5 cut(s) 134, 536, 916, 1167, 1422
PfeI GAWTC 6 cut(s) 44, 273, 537, 830, 1068, 1257
PflMI CCANNNNNTGG 1 cut(s) 646
PfoI TCCNGGA 1 cut(s) 95
PkrI GCNGC 2 cut(s) 254, 1144
PleI GAGTC 2 cut(s) 1548, 1612
PmeI GTTTAAAC 1 cut(s) 705
PpsI GAGTC 2 cut(s) 1548, 1612
PscI ACATGT 2 cut(s) 853, 1077
PsiI TTATAA 2 cut(s) 621, 1275
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 194, 1573
PspGI CCWGG 2 cut(s) 194, 1573
PspN4I GGNNCC 4 cut(s) 137, 638, 1119, 1241
PspOMI GGGCCC 1 cut(s) 636
PspPI GGNCC 4 cut(s) 636, 637, 1359, 1478
PstI CTGCAG 1 cut(s) 1594
PsuI RGATCY 2 cut(s) 1239, 1472
RsaI GTAC 4 cut(s) 521, 552, 616, 981
RsaNI GTAC 4 cut(s) 520, 551, 615, 980
SacI GAGCTC 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 715
SaqAI TTAA 7 cut(s) 110, 156, 264, 704, 1134, 1149, 1505
SatI GCNGC 2 cut(s) 253, 1143
Sau3AI GATC 6 cut(s) 236, 372, 887, 1239, 1457, 1472
Sau96I GGNCC 4 cut(s) 636, 637, 1359, 1478
ScaI AGTACT 1 cut(s) 981
SchI GAGTC 2 cut(s) 1549, 1613
ScrFI CCNGG 4 cut(s) 97, 196, 1363, 1575
SduI GDGCHC 5 cut(s) 640, 727, 778, 1180, 1340
SfaNI GCATC 5 cut(s) 101, 136, 197, 451, 1533
SfcI CTRYAG 2 cut(s) 1005, 1590
SfuI TTCGAA 1 cut(s) 592
SinI GGWCC 2 cut(s) 1359, 1478
SmlI CTYRAG 2 cut(s) 1463, 1504
SmoI CTYRAG 2 cut(s) 1463, 1504
SseBI AGGCCT 1 cut(s) 1422
SsiI CCGC 4 cut(s) 205, 252, 972, 1548
SspI AATATT 1 cut(s) 917
SspMI CTAG 1 cut(s) 1196
SstI GAGCTC 1 cut(s) 727
StuI AGGCCT 1 cut(s) 1422
StyD4I CCNGG 4 cut(s) 95, 194, 1361, 1573
StyI CCWWGG 3 cut(s) 256, 277, 814
TaaI ACNGT 2 cut(s) 605, 1168
TaiI ACGT 2 cut(s) 1316, 1380
TaqI TCGA 4 cut(s) 8, 47, 592, 1155
TatI WGTACW 3 cut(s) 519, 550, 979
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 6 cut(s) 44, 273, 537, 830, 1068, 1257
Tru1I TTAA 7 cut(s) 110, 156, 264, 704, 1134, 1149, 1505
Tru9I TTAA 7 cut(s) 110, 156, 264, 704, 1134, 1149, 1505
TscAI CASTG 2 cut(s) 874, 1599
TseFI GTSAC 1 cut(s) 231
TseI GCWGC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 231
TspDTI ATGAA 9 cut(s) 77, 240, 365, 656, 1076, 1176, 1556, 1641, 1641
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 2 cut(s) 874, 1599
Van91I CCANNNNNTGG 1 cut(s) 646
Vha464I CTTAAG 1 cut(s) 1504
VneI GTGCAC 1 cut(s) 774
VpaK11BI GGWCC 2 cut(s) 1359, 1478
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 4 cut(s) 176, 486, 1366, 1483
XceI RCATGY 2 cut(s) 857, 1081
XmnI GAANNNNTTC 5 cut(s) 134, 536, 916, 1167, 1422
XspI CTAG 1 cut(s) 1196
ZrmI AGTACT 1 cut(s) 981
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.