RchiOBHm_Chr1g0371431

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012162
Physical Location & Seq
Reverse (-)
60615970 .. 60619336
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59551

Sequence Viewer

Length: 1614 bp
ATGGTTTCGACTGATAATGGAAACCAAAAAGAGGAAATTGAAGGCTCTAATGGTGGTGGAGTGAAGGCTCCAGTTCCCTTGGACAACCTTTCTGTCTCTAGTAGGAATTTGTTAAGCGATGAAAAGCCTCAGAGGAGGTTTCATGATGTTATTTCTGATAGGGTAAGGTTCCTGAAATTTGGCTCTGCATCTGCCAGATTTAAGCGGTTAGCTATGGATAGAGATGAGATTTCACGCTCGGTGGCTTCGCCAAGGGGACATGGTTTCAGAGAAAGGATCAGTGGGGTCTTTTCCAAGAAAATTGATTGGCCTTCGCTCATGAAAATGAGCAAAGAGTGGATTAGAGAACCAATGAACATGGCTCTTTTTCTGTGGATTGCCTGTGTTGCTGTTTCTGGTGCAATTTTGTTTCTTGTCATGACTGGAATGTTGAACCATGCACTACCAAAGAAGTCCCAGAGAGATGCATGGTTCGAGCGATTTTACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCTAGACTCAGAAAGATTTACTGTAAAAATGGGACTTATAAGCCACATGAATGGGCACACATGATGGTTGTTGTTGTTCTGCTCCATGTGAATTGCTTTGCGCAATATGCGCTTTGTGGTTTAAACTTGGGTTACAAAAGATCTGAACGACCAGCCATAGGAGTTGGAATATGTGTATCTGTTGCAATTGGTGCACCTGCAATTGCTGGTATATACAACATTGTCAGTCCCCTTGGGAAGGATTATGACATGGATGAGGAAGCGCAGCTTCAGTTTACCGCTGATGAAGCAGCTGAAAAGTTGAGGTTAAAGCGATTAGTGAGAAGGTATTCTTTTGCATCCAGGGATGAACATCGAATCATTGAGGCTGCCCCAAAATGGACTGGAGGTATACTGGATATTTGGGATGACATTTCTCTTGCATATGTCTCACTGTTCTGTAGCTTTTGTGTATTTGGGTGGAATATGGACAGACTTGGGTTCGGGAACATGTATGTTCATATTCTGACATTTATTCTGTTTTGCGTGGCTCCTTTCTGGATCTTTAACTTGGCTGCTGTTAATATTGACAATGAGACTGTTCGGGAGTCTCTTGCAGTAACTGGCATTATTCTCTGTGTTTTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTTAACTTGCCTTCTTATAACTTCTGTTTTGGTGAACCGGCAGTGTCTGATTGCGCATTGTGGCTTTTCTGTTGTTGGTGTTCTCTTGCTCAGGAAGTGAGGACGGGGAATTCCTATGACATTGTAGAAGATAAGTTTTGTAAAAAAGAAACAGATATTGGGAGCCAGCTACCAATGTCACCCTTGCCCCGTGAAGATGGAGTGGGTCAACTTCGGTCTGGTCCAAGTTCGCCTTTGGGGAACTACTCAAGCCCAACTAAGACCGGCATGGTTGGTTCTCCAAGTCCTAGCAGATTTTCAAAGGGACTGTATAGTCCAGATAGGGCGCTTTCTTCGGTGAAAGAAGAATCTCATCCAACAGGTAAGGATGAAACTATGACCGCACCTGCACCCTCGGTGATACAAAGAGAAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

537

Amino Acids

60.38

Weight (kDa)

8.75

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 112 - 159 6.8e-13 Protein of unknown function (DUF2985)
PLAC8 PF04749 302 - 432 2.3e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 1212
AarI CACCTGC 2 cut(s) 730, 1588
AasI GACNNNNNNGTC 1 cut(s) 1506
Acc16I TGCGCA 2 cut(s) 627, 1249
Acc36I ACCTGC 2 cut(s) 730, 1588
AccI GTMKAC 1 cut(s) 916
AciI CCGC 3 cut(s) 205, 804, 1575
AclWI GGATC 4 cut(s) 284, 1073, 1171, 1184
AcsI RAATTY 3 cut(s) 106, 176, 1303
AcuI CTGAAG 1 cut(s) 779
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 4 cut(s) 31, 683, 763, 903
AflIII ACRYGT 1 cut(s) 1014
AgsI TTSAA 3 cut(s) 41, 433, 1494
AjnI CCWGG 1 cut(s) 866
AjuI GAANNNNNNNTTGG 2 cut(s) 1335, 1367
AluBI AGCT 5 cut(s) 212, 793, 818, 969, 1363
AluI AGCT 5 cut(s) 212, 793, 818, 969, 1363
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 4 cut(s) 100, 958, 1094, 1119
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 4 cut(s) 284, 1073, 1171, 1184
AlwNI CAGNNNCTG 2 cut(s) 1127, 1241
AoxI GGCC 1 cut(s) 308
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 4 cut(s) 790, 815, 893, 1079
ApoI RAATTY 3 cut(s) 106, 176, 1303
Asp700I GAANNNNTTC 1 cut(s) 853
AspLEI GCGC 5 cut(s) 628, 637, 790, 1250, 1522
AspS9I GGNCC 1 cut(s) 1415
AsuHPI GGTGA 4 cut(s) 1238, 1365, 1543, 1603
AvaII GGWCC 1 cut(s) 1415
BaeGI GKGCMC 2 cut(s) 583, 721
BamHI GGATCC 1 cut(s) 1176
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 4 cut(s) 802, 827, 880, 1066
BccI CCATC 3 cut(s) 501, 583, 1385
BciT130I CCWGG 1 cut(s) 868
BcoDI GTCTC 4 cut(s) 100, 958, 1094, 1119
BfaI CTAG 3 cut(s) 99, 528, 1482
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 1 cut(s) 1523
BfuAI ACCTGC 2 cut(s) 730, 1588
BglII AGATCT 1 cut(s) 665
BisI GCNGC 4 cut(s) 791, 816, 894, 1080
BlsI GCNGC 4 cut(s) 792, 817, 895, 1081
Bme1390I CCNGG 1 cut(s) 868
Bme18I GGWCC 1 cut(s) 1415
BmgT120I GGNCC 1 cut(s) 1415
BmiI GGNNCC 5 cut(s) 69, 170, 1056, 1178, 1358
BmrFI CCNGG 1 cut(s) 868
BmsI GCATC 3 cut(s) 197, 454, 872
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 2 cut(s) 54, 930
Bpu10I CCTNAGC 1 cut(s) 1284
BpuEI CTTGAG 1 cut(s) 1426
BsaBI GATNNNNATC 1 cut(s) 876
BsaJI CCNNGG 5 cut(s) 78, 251, 757, 867, 1587
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 683, 763, 903
Bse118I RCCGGY 2 cut(s) 1231, 1457
Bse1I ACTGG 5 cut(s) 71, 427, 913, 924, 1132
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 1 cut(s) 868
BseDI CCNNGG 5 cut(s) 78, 251, 757, 867, 1587
BseGI GGATG 6 cut(s) 784, 863, 877, 937, 1546, 1567
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 4 cut(s) 31, 683, 763, 903
BseMII CTCAG 3 cut(s) 143, 547, 1298
BseNI ACTGG 5 cut(s) 71, 427, 913, 924, 1132
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 2 cut(s) 583, 721
BseXI GCAGC 4 cut(s) 802, 827, 880, 1066
BsgI GTGCAG 1 cut(s) 1566
BshFI GGCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 2 cut(s) 1232, 1458
BslFI GGGAC 5 cut(s) 270, 439, 571, 738, 1512
BslI CCNNNNNNNGG 4 cut(s) 31, 683, 763, 903
BsmAI GTCTC 4 cut(s) 100, 958, 1094, 1119
BsmFI GGGAC 5 cut(s) 270, 439, 571, 738, 1512
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 583, 721
Bsp143I GATC 4 cut(s) 276, 665, 1065, 1176
BspACI CCGC 3 cut(s) 205, 804, 1575
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 3 cut(s) 142, 546, 1297
BspHI TCATGA 3 cut(s) 142, 318, 417
BspLI GGNNCC 5 cut(s) 69, 170, 1056, 1178, 1358
BspMI ACCTGC 2 cut(s) 730, 1588
BspPI GGATC 4 cut(s) 284, 1073, 1171, 1184
BsrFI RCCGGY 2 cut(s) 1231, 1457
BsrI ACTGG 5 cut(s) 71, 427, 913, 924, 1132
BssAI RCCGGY 2 cut(s) 1231, 1457
BssECI CCNNGG 5 cut(s) 78, 251, 757, 867, 1587
BssMI GATC 4 cut(s) 276, 665, 1065, 1176
BssNAI GTATAC 1 cut(s) 917
BssT1I CCWWGG 3 cut(s) 78, 251, 757
Bst1107I GTATAC 1 cut(s) 917
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 4 cut(s) 548, 960, 1105, 1503
BstAPI GCANNNNNTGC 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 1361
BstDEI CTNAG 4 cut(s) 129, 533, 1284, 1452
BstENI CCTNNNNNAGG 1 cut(s) 761
BstF5I GGATG 6 cut(s) 784, 863, 877, 937, 1546, 1567
BstH2I RGCGCY 1 cut(s) 1523
BstHHI GCGC 5 cut(s) 628, 637, 790, 1250, 1522
BstKTI GATC 4 cut(s) 279, 668, 1068, 1179
BstMAI GTCTC 4 cut(s) 100, 958, 1094, 1119
BstMBI GATC 4 cut(s) 276, 665, 1065, 1176
BstMWI GCNNNNNNNGC 5 cut(s) 386, 632, 634, 716, 812
BstNI CCWGG 1 cut(s) 868
BstNSI RCATGY 1 cut(s) 1018
BstSCI CCNGG 1 cut(s) 866
BstSFI CTRYAG 1 cut(s) 964
BstSLI GKGCMC 2 cut(s) 583, 721
BstV1I GCAGC 4 cut(s) 802, 827, 880, 1066
BstV2I GAAGAC 1 cut(s) 525
BstX2I RGATCY 3 cut(s) 665, 1065, 1176
BstXI CCANNNNNNTGG 1 cut(s) 576
BstYI RGATCY 3 cut(s) 665, 1065, 1176
BstZ17I GTATAC 1 cut(s) 917
BsuRI GGCC 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 6 cut(s) 784, 863, 877, 937, 1546, 1567
BtsI GCAGTG 1 cut(s) 1242
BtsIMutI CAGTG 3 cut(s) 286, 956, 1242
BveI ACCTGC 2 cut(s) 730, 1588
Cac8I GCNNGC 1 cut(s) 1361
CaiI CAGNNNCTG 2 cut(s) 1127, 1241
CciI TCATGA 3 cut(s) 142, 318, 417
CfoI GCGC 5 cut(s) 628, 637, 790, 1250, 1522
Cfr10I RCCGGY 2 cut(s) 1231, 1457
Cfr13I GGNCC 1 cut(s) 1415
Csp6I GTAC 1 cut(s) 494
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 4 cut(s) 129, 533, 1284, 1452
DpnI GATC 4 cut(s) 278, 667, 1067, 1178
DpnII GATC 4 cut(s) 276, 665, 1065, 1176
DraI TTTAAA 1 cut(s) 648
DrdI GACNNNNNNGTC 1 cut(s) 1506
DseDI GACNNNNNNGTC 1 cut(s) 1506
Eco130I CCWWGG 3 cut(s) 78, 251, 757
Eco47I GGWCC 1 cut(s) 1415
Eco57I CTGAAG 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 761
EcoRI GAATTC 1 cut(s) 1303
EcoRII CCWGG 1 cut(s) 866
EcoT14I CCWWGG 3 cut(s) 78, 251, 757
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 3 cut(s) 78, 251, 757
FalI AAGNNNNNCTT 6 cut(s) 777, 809, 841, 873, 1411, 1443
FaqI GGGAC 5 cut(s) 270, 439, 571, 738, 1512
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 4 cut(s) 791, 816, 894, 1080
FokI GGATG 6 cut(s) 791, 850, 884, 944, 1533, 1574
Fsp4HI GCNGC 4 cut(s) 791, 816, 894, 1080
FspBI CTAG 3 cut(s) 99, 528, 1482
FspI TGCGCA 2 cut(s) 627, 1249
GlaI GCGC 5 cut(s) 627, 636, 789, 1249, 1521
GluI GCNGC 4 cut(s) 791, 816, 894, 1080
GsuI CTGGAG 2 cut(s) 54, 930
HaeII RGCGCY 1 cut(s) 1523
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 1232, 1458
HhaI GCGC 5 cut(s) 628, 637, 790, 1250, 1522
Hin6I GCGC 5 cut(s) 626, 635, 788, 1248, 1520
HinP1I GCGC 5 cut(s) 626, 635, 788, 1248, 1520
HincII GTYRAC 1 cut(s) 1403
HindII GTYRAC 1 cut(s) 1403
HinfI GANTC 4 cut(s) 531, 882, 1112, 1541
HpaII CCGG 2 cut(s) 1232, 1458
HphI GGTGA 4 cut(s) 1238, 1365, 1543, 1603
Hpy166II GTNNAC 5 cut(s) 719, 801, 917, 1229, 1403
Hpy188I TCNGA 7 cut(s) 132, 157, 269, 536, 670, 1032, 1243
Hpy8I GTNNAC 5 cut(s) 719, 801, 917, 1229, 1403
HpyAV CCTTC 6 cut(s) 35, 58, 321, 757, 843, 1215
HpyCH4III ACNGT 4 cut(s) 548, 960, 1105, 1503
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 632, 634, 716, 812
HpyF3I CTNAG 4 cut(s) 129, 533, 1284, 1452
HspAI GCGC 5 cut(s) 626, 635, 788, 1248, 1520
Kzo9I GATC 4 cut(s) 276, 665, 1065, 1176
LmnI GCTCC 4 cut(s) 73, 612, 1060, 1356
Lsp1109I GCAGC 4 cut(s) 802, 827, 880, 1066
LweI GCATC 3 cut(s) 197, 454, 872
MaeI CTAG 3 cut(s) 99, 528, 1482
MaeIII GTNAC 3 cut(s) 656, 1123, 1371
MalI GATC 4 cut(s) 278, 667, 1067, 1178
MboI GATC 4 cut(s) 276, 665, 1065, 1176
MboII GAAGA 5 cut(s) 530, 1334, 1400, 1518, 1550
MfeI CAATTG 2 cut(s) 711, 726
MflI RGATCY 3 cut(s) 665, 1065, 1176
MhlI GDGCHC 2 cut(s) 583, 721
MluCI AATT 9 cut(s) 36, 106, 176, 300, 402, 616, 711, 726, 1303
MlyI GAGTC 2 cut(s) 525, 1121
MmeI TCCRAC 2 cut(s) 670, 1574
Mph1103I ATGCAT 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 853
MseI TTAA 7 cut(s) 113, 201, 647, 833, 1071, 1086, 1197
MslI CAYNNNNRTG 2 cut(s) 323, 574
MspA1I CMGCKG 2 cut(s) 806, 818
MspI CCGG 2 cut(s) 1232, 1458
MspR9I CCNGG 1 cut(s) 868
MssI GTTTAAAC 1 cut(s) 648
MunI CAATTG 2 cut(s) 711, 726
MvaI CCWGG 1 cut(s) 868
MwoI GCNNNNNNNGC 5 cut(s) 386, 632, 634, 716, 812
NdeI CATATG 1 cut(s) 949
NdeII GATC 4 cut(s) 276, 665, 1065, 1176
NlaIV GGNNCC 5 cut(s) 69, 170, 1056, 1178, 1358
NmuCI GTSAC 1 cut(s) 1371
NsbI TGCGCA 2 cut(s) 627, 1249
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 1 cut(s) 1018
PagI TCATGA 3 cut(s) 142, 318, 417
PaqCI CACCTGC 2 cut(s) 730, 1588
PciI ACATGT 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 2 cut(s) 882, 1541
PkrI GCNGC 4 cut(s) 792, 817, 895, 1081
PleI GAGTC 2 cut(s) 525, 1120
PmeI GTTTAAAC 1 cut(s) 648
PpsI GAGTC 2 cut(s) 525, 1120
PscI ACATGT 1 cut(s) 1014
PsiI TTATAA 2 cut(s) 564, 1212
Psp6I CCWGG 1 cut(s) 866
PspGI CCWGG 1 cut(s) 866
PspN4I GGNNCC 5 cut(s) 69, 170, 1056, 1178, 1358
PspPI GGNCC 1 cut(s) 1415
PstNI CAGNNNCTG 2 cut(s) 1127, 1241
PsuI RGATCY 3 cut(s) 665, 1065, 1176
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 2 cut(s) 323, 574
SaqAI TTAA 7 cut(s) 113, 201, 647, 833, 1071, 1086, 1197
SatI GCNGC 4 cut(s) 791, 816, 894, 1080
Sau3AI GATC 4 cut(s) 276, 665, 1065, 1176
Sau96I GGNCC 1 cut(s) 1415
SchI GAGTC 2 cut(s) 525, 1121
ScrFI CCNGG 1 cut(s) 868
SduI GDGCHC 2 cut(s) 583, 721
SfaNI GCATC 3 cut(s) 197, 454, 872
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 1 cut(s) 1415
SmiMI CAYNNNNRTG 2 cut(s) 323, 574
SmlI CTYRAG 1 cut(s) 1441
SmoI CTYRAG 1 cut(s) 1441
Sse9I AATT 9 cut(s) 36, 106, 176, 300, 402, 616, 711, 726, 1303
SsiI CCGC 3 cut(s) 205, 804, 1575
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 99, 528, 1482
StyD4I CCNGG 1 cut(s) 866
StyI CCWWGG 3 cut(s) 78, 251, 757
TaaI ACNGT 4 cut(s) 548, 960, 1105, 1503
TaqI TCGA 3 cut(s) 8, 474, 880
TaqII GACCGA 1 cut(s) 1398
TasI AATT 9 cut(s) 36, 106, 176, 300, 402, 616, 711, 726, 1303
TatI WGTACW 1 cut(s) 493
TfiI GAWTC 2 cut(s) 882, 1541
Tru1I TTAA 7 cut(s) 113, 201, 647, 833, 1071, 1086, 1197
Tru9I TTAA 7 cut(s) 113, 201, 647, 833, 1071, 1086, 1197
TscAI CASTG 3 cut(s) 286, 963, 1242
TseFI GTSAC 1 cut(s) 1371
TseI GCWGC 4 cut(s) 790, 815, 893, 1079
Tsp45I GTSAC 1 cut(s) 1371
TspDTI ATGAA 9 cut(s) 131, 135, 335, 368, 588, 825, 888, 1013, 1578
TspRI CASTG 3 cut(s) 286, 963, 1242
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 1 cut(s) 1415
XagI CCTNNNNNAGG 1 cut(s) 761
XapI RAATTY 3 cut(s) 106, 176, 1303
XbaI TCTAGA 1 cut(s) 527
XceI RCATGY 1 cut(s) 1018
XmiI GTMKAC 1 cut(s) 916
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 3 cut(s) 99, 528, 1482
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.