Prupe.2G259100_v2.0.a1

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012162
Physical Location & Seq
Reverse (-)
26957959 .. 26959754
1796 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G259100.1

Sequence Viewer

Length: 1689 bp
ATGGTTTCAACTGATAATGGAAACCACAAAGAGGAAATTGAAGAATCTAATGGCAGTGAAGTGAAGGCTCAGATCCCCTTTGACGTTTCAATATCTCAGAAGACATTGTTAAGCAATGTAAAGTCTCCAAAAAGGTTCCCTGATGCTGTTCCTAATAGAATAAGGTTCCTCAAATTTGGTTCTGCATCTGCAAGATTCAAGCGCATAGCTGAGGAGAGAGATGAGATCTCAAGATCTGTGGTTTCGATTGGTCATGGTTTTAAAGAACGAATCAACGGGGTTTTTTCCCGGAAAATTGATTGGGTTTCACTCATGAAAATGAGCAAACAATGGATTAGGAACCCAATGAACATGGCTCTTTTTGTGTGGATCACCTGTGTTGCTGTTTCAGGTGCAATTCTGTTTCTTGTCATGACTGGAATGTTGAACAAGGCAATACCAAAGAAGTCCCAAAGAAATGCATGGTTTGAAGTCAATAATCAAATTCTCAATGCACTTTTTACTCTTATGTGTTTGTACCAACATCCAAAGCGGTTCTACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCTAGACTTAGAAAGATTTACTGTAAAAATGGGACTTACAAGCCACATGAATGGGCCCACATGATGGTGGTTGTTGGTCTTCTTCATTTGAATTGCTTTGGTCAATATGCTCTTTGCGGCTTAAACCTGGGTTACAGGAGATCTCAACGACCACCCATAGGAGTTGGAATATGTATAACTTTTGCTATTGGTGCCCCTGCATTTGCTGGTATATACAACATTATCAGTCCCCTTGGGAAGGATTATGATTCAGAGTTGGTGGATGAGGAAGCACAGGTTCGGATTGGCACCGATGAAGGACAAGAAAGGTTAAGGTTAAAATCATTTGAGAGAAAATATTCTTTTGCAGCAAGGACTGAACAGCGTGTCGTTGAGAATAGACCACAATGGAGCGGAGGGCTACTTGATATTTGGGATGATATTTCTCTAGCATATCTTTCACTGTTTTGTGGTTTTTGTGTGTTCGGCTGGAATATGGAGAGGCTTGGATTTGGGAACATGTATGTTCACATTATGACATTTGTTCTGTTTTGCATGGCTCCCTTCTGGATTTTTAATTTGGCTGCTGTTAATATAGACAATGAGACTGTTCGGGAGGCTCTTGGAGTTACTGGACTTGTTCTCTGTGTATTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTCAGTTTGCCTTCTTATAACTTCTGTTTTGGTGAACCAGCACTGGCTGATTGCACGCTGTGGCTTTGCTGTTGTTGGTGCTCTCTTGCTCAGGAAGTGAGGACTGGGAATTCCTATGACATTGTAGAAGAAAAGTTTTATAGAAAACAAACGGACAGTGGCAGCCAGCTACCAATTTCACCTTTGGCTCGTGAGGATGGAGTGGTTGAATTTAGATCTGCTCCAAATTCTCCTCTGGGGAGCTACTCAAGCCCAACTAAGTTTAGCGTTGGTGGTTCTCCAAGTCCTAGCAGACTTTCAAAGGGATTTTATAGTCCAGATAGGCCACTTTCCACAGTGAAAGAAGAGTCTTATGTAAGAGGTAAGGATGAAACTATGACCCCACCTGCACCCTCGCTGATAGAAAGGGAGGGTACAGAGCCCCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

563

Amino Acids

63.81

Weight (kDa)

8.95

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1275
AarI CACCTGC 1 cut(s) 1651
Acc36I ACCTGC 1 cut(s) 1651
AccB1I GGYRCC 2 cut(s) 770, 866
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 972
AciI CCGC 3 cut(s) 532, 696, 972
AclWI GGATC 4 cut(s) 67, 377, 1234, 1247
AcsI RAATTY 5 cut(s) 173, 483, 1366, 1466, 1483
AdeI CACNNNGTG 1 cut(s) 1317
AfaI GTAC 3 cut(s) 518, 549, 1672
AfiI CCNNNNNNNGG 4 cut(s) 31, 643, 737, 817
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 9 cut(s) 9, 41, 90, 199, 427, 470, 670, 1466, 1557
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 3 cut(s) 209, 1426, 1500
AluI AGCT 3 cut(s) 209, 1426, 1500
Alw21I GWGCWC 1 cut(s) 1340
Alw26I GTCTC 2 cut(s) 129, 1157
AlwI GGATC 4 cut(s) 67, 377, 1234, 1247
AoxI GGCC 2 cut(s) 633, 1580
ApaI GGGCCC 1 cut(s) 637
ApeKI GCWGC 3 cut(s) 926, 1142, 1419
ApoI RAATTY 5 cut(s) 173, 483, 1366, 1466, 1483
Asp700I GAANNNNTTC 1 cut(s) 916
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 633, 634
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 3 cut(s) 364, 1301, 1428
BaeGI GKGCMC 2 cut(s) 637, 775
BamHI GGATCC 1 cut(s) 1239
BanI GGYRCC 2 cut(s) 770, 866
BanII GRGCYC 2 cut(s) 637, 1680
BauI CACGAG 1 cut(s) 1446
BbsI GAAGAC 3 cut(s) 107, 579, 650
Bbv12I GWGCWC 1 cut(s) 1340
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 938, 1129, 1431
BccI CCATC 3 cut(s) 555, 637, 1448
BciT130I CCWGG 1 cut(s) 707
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 129, 1157
BfaI CTAG 3 cut(s) 582, 1007, 1545
BfuAI ACCTGC 1 cut(s) 1651
BglII AGATCT 4 cut(s) 225, 233, 719, 1472
BisI GCNGC 4 cut(s) 697, 927, 1143, 1420
BlsI GCNGC 4 cut(s) 698, 928, 1144, 1421
Bme1390I CCNGG 2 cut(s) 289, 707
BmgT120I GGNCC 2 cut(s) 633, 634
BmiI GGNNCC 8 cut(s) 137, 167, 341, 635, 772, 868, 1119, 1241
BmrFI CCNGG 2 cut(s) 289, 707
BmrI ACTGGG 1 cut(s) 1371
BmsI GCATC 2 cut(s) 133, 194
BmuI ACTGGG 1 cut(s) 1371
BpiI GAAGAC 3 cut(s) 107, 579, 650
Bpu10I CCTNAGC 2 cut(s) 210, 1347
BpuEI CTTGAG 2 cut(s) 214, 1489
BpuMI CCSGG 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 706, 811
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 643, 737, 817
Bse1I ACTGG 4 cut(s) 421, 1195, 1305, 1366
Bse3DI GCAATG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 2 cut(s) 706, 811
BseGI GGATG 5 cut(s) 523, 847, 1000, 1459, 1630
BseLI CCNNNNNNNGG 4 cut(s) 31, 643, 737, 817
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 4 cut(s) 83, 110, 201, 1361
BseNI ACTGG 4 cut(s) 421, 1195, 1305, 1366
BseRI GAGGAG 2 cut(s) 227, 1479
BseSI GKGCMC 2 cut(s) 637, 775
BseXI GCAGC 3 cut(s) 938, 1129, 1431
BsgI GTGCAG 1 cut(s) 1629
BshFI GGCC 2 cut(s) 635, 1582
BshNI GGYRCC 2 cut(s) 770, 866
BsiHKAI GWGCWC 1 cut(s) 1340
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 3 cut(s) 433, 625, 792
BslI CCNNNNNNNGG 4 cut(s) 31, 643, 737, 817
BsmAI GTCTC 2 cut(s) 129, 1157
BsmFI GGGAC 3 cut(s) 433, 625, 792
BsnI GGCC 2 cut(s) 635, 1582
Bsp120I GGGCCC 1 cut(s) 633
Bsp1286I GDGCHC 4 cut(s) 637, 775, 1340, 1680
Bsp143I GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
BspACI CCGC 3 cut(s) 532, 696, 972
BspANI GGCC 2 cut(s) 635, 1582
BspCNI CTCAG 4 cut(s) 82, 109, 202, 1360
BspHI TCATGA 2 cut(s) 312, 411
BspLI GGNNCC 8 cut(s) 137, 167, 341, 635, 772, 868, 1119, 1241
BspMI ACCTGC 1 cut(s) 1651
BspPI GGATC 4 cut(s) 67, 377, 1234, 1247
BspT107I GGYRCC 2 cut(s) 770, 866
BsrBI CCGCTC 1 cut(s) 972
BsrDI GCAATG 1 cut(s) 121
BsrI ACTGG 4 cut(s) 421, 1195, 1305, 1366
BssECI CCNNGG 2 cut(s) 706, 811
BssMI GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
BssSI CACGAG 1 cut(s) 1446
BssT1I CCWWGG 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 1446
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 5 cut(s) 602, 1023, 1168, 1415, 1594
Bst6I CTCTTC 1 cut(s) 1596
BstC8I GCNNGC 2 cut(s) 1313, 1424
BstDEI CTNAG 6 cut(s) 69, 96, 210, 587, 1347, 1515
BstENI CCTNNNNNAGG 1 cut(s) 815
BstF5I GGATG 5 cut(s) 523, 847, 1000, 1459, 1630
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 7 cut(s) 75, 228, 236, 372, 722, 1242, 1475
BstMAI GTCTC 2 cut(s) 129, 1157
BstMBI GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
BstMWI GCNNNNNNNGC 2 cut(s) 770, 1506
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 2 cut(s) 287, 705
BstSLI GKGCMC 2 cut(s) 637, 775
BstV1I GCAGC 3 cut(s) 938, 1129, 1431
BstV2I GAAGAC 3 cut(s) 107, 579, 650
BstX2I RGATCY 6 cut(s) 72, 225, 233, 719, 1239, 1472
BstXI CCANNNNNNTGG 1 cut(s) 630
BstYI RGATCY 6 cut(s) 72, 225, 233, 719, 1239, 1472
BsuRI GGCC 2 cut(s) 635, 1582
BtsCI GGATG 5 cut(s) 523, 847, 1000, 1459, 1630
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 5 cut(s) 61, 1019, 1298, 1420, 1599
BveI ACCTGC 1 cut(s) 1651
Cac8I GCNNGC 2 cut(s) 1313, 1424
CciI TCATGA 2 cut(s) 312, 411
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 633, 634
Csp6I GTAC 3 cut(s) 517, 548, 1671
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 9 cut(s) 254, 313, 352, 412, 462, 626, 640, 1078, 1114
CviQI GTAC 3 cut(s) 517, 548, 1671
DdeI CTNAG 6 cut(s) 69, 96, 210, 587, 1347, 1515
DpnI GATC 7 cut(s) 74, 227, 235, 371, 721, 1241, 1474
DpnII GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
DraI TTTAAA 1 cut(s) 262
DraIII CACNNNGTG 1 cut(s) 1317
Eam1104I CTCTTC 1 cut(s) 1596
EarI CTCTTC 1 cut(s) 1596
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 2 cut(s) 637, 1680
EcoNI CCTNNNNNAGG 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 1 cut(s) 811
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 637, 1680
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 9 cut(s) 257, 316, 355, 415, 465, 629, 643, 1081, 1117
FaqI GGGAC 3 cut(s) 433, 625, 792
FatI CATG 9 cut(s) 253, 312, 351, 411, 461, 625, 639, 1077, 1113
Fnu4HI GCNGC 4 cut(s) 697, 927, 1143, 1420
FokI GGATG 5 cut(s) 510, 854, 1007, 1466, 1637
FriOI GRGCYC 2 cut(s) 637, 1680
Fsp4HI GCNGC 4 cut(s) 697, 927, 1143, 1420
FspBI CTAG 3 cut(s) 582, 1007, 1545
GlaI GCGC 1 cut(s) 203
GluI GCNGC 4 cut(s) 697, 927, 1143, 1420
HaeIII GGCC 2 cut(s) 635, 1582
HapII CCGG 1 cut(s) 289
HhaI GCGC 1 cut(s) 204
Hin1II CATG 9 cut(s) 257, 316, 355, 415, 465, 629, 643, 1081, 1117
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HinfI GANTC 6 cut(s) 44, 195, 270, 827, 1257, 1604
HpaII CCGG 1 cut(s) 289
HphI GGTGA 3 cut(s) 364, 1301, 1428
Hpy166II GTNNAC 2 cut(s) 1087, 1292
Hpy188I TCNGA 4 cut(s) 72, 99, 832, 861
Hpy188III TCNNGA 9 cut(s) 231, 313, 412, 582, 1126, 1172, 1349, 1448, 1574
Hpy8I GTNNAC 2 cut(s) 1087, 1292
HpyAV CCTTC 5 cut(s) 58, 811, 869, 1132, 1278
HpyCH4III ACNGT 5 cut(s) 602, 1023, 1168, 1415, 1594
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 2 cut(s) 770, 1506
HpyF3I CTNAG 6 cut(s) 69, 96, 210, 587, 1347, 1515
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 9 cut(s) 257, 316, 355, 415, 465, 629, 643, 1081, 1117
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
LmnI GCTCC 4 cut(s) 969, 1123, 1483, 1497
Lsp1109I GCAGC 3 cut(s) 938, 1129, 1431
LweI GCATC 2 cut(s) 133, 194
MaeI CTAG 3 cut(s) 582, 1007, 1545
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 710, 1186
MalI GATC 7 cut(s) 74, 227, 235, 371, 721, 1241, 1474
MbiI CCGCTC 1 cut(s) 972
MboI GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
MboII GAAGA 7 cut(s) 53, 112, 584, 650, 653, 1397, 1613
MflI RGATCY 6 cut(s) 72, 225, 233, 719, 1239, 1472
MhlI GDGCHC 4 cut(s) 637, 775, 1340, 1680
MlyI GAGTC 1 cut(s) 1613
MmeI TCCRAC 1 cut(s) 724
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 916
MseI TTAA 7 cut(s) 110, 261, 701, 890, 896, 1134, 1149
MslI CAYNNNNRTG 3 cut(s) 317, 628, 644
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 2 cut(s) 289, 707
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 2 cut(s) 770, 1506
NciI CCSGG 1 cut(s) 289
NdeII GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
NlaIII CATG 9 cut(s) 257, 316, 355, 415, 465, 629, 643, 1081, 1117
NlaIV GGNNCC 8 cut(s) 137, 167, 341, 635, 772, 868, 1119, 1241
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 1081
PagI TCATGA 2 cut(s) 312, 411
PaqCI CACCTGC 1 cut(s) 1651
PciI ACATGT 1 cut(s) 1077
PdmI GAANNNNTTC 1 cut(s) 916
PfeI GAWTC 5 cut(s) 44, 195, 270, 827, 1257
PflMI CCANNNNNTGG 1 cut(s) 643
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 4 cut(s) 698, 928, 1144, 1421
PleI GAGTC 1 cut(s) 1612
PpsI GAGTC 1 cut(s) 1612
PscI ACATGT 1 cut(s) 1077
PsiI TTATAA 1 cut(s) 1275
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 8 cut(s) 137, 167, 341, 635, 772, 868, 1119, 1241
PspOMI GGGCCC 1 cut(s) 633
PspPI GGNCC 2 cut(s) 633, 634
PsuI RGATCY 6 cut(s) 72, 225, 233, 719, 1239, 1472
RsaI GTAC 3 cut(s) 518, 549, 1672
RsaNI GTAC 3 cut(s) 517, 548, 1671
RseI CAYNNNNRTG 3 cut(s) 317, 628, 644
SaqAI TTAA 7 cut(s) 110, 261, 701, 890, 896, 1134, 1149
SatI GCNGC 4 cut(s) 697, 927, 1143, 1420
Sau3AI GATC 7 cut(s) 72, 225, 233, 369, 719, 1239, 1472
Sau96I GGNCC 2 cut(s) 633, 634
SchI GAGTC 1 cut(s) 1613
ScrFI CCNGG 2 cut(s) 289, 707
SduI GDGCHC 4 cut(s) 637, 775, 1340, 1680
SfaNI GCATC 2 cut(s) 133, 194
SmiMI CAYNNNNRTG 3 cut(s) 317, 628, 644
SmlI CTYRAG 2 cut(s) 229, 1504
SmoI CTYRAG 2 cut(s) 229, 1504
SsiI CCGC 3 cut(s) 532, 696, 972
SspI AATATT 1 cut(s) 917
SspMI CTAG 3 cut(s) 582, 1007, 1545
StyD4I CCNGG 2 cut(s) 287, 705
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 5 cut(s) 602, 1023, 1168, 1415, 1594
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 245
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 5 cut(s) 44, 195, 270, 827, 1257
Tru1I TTAA 7 cut(s) 110, 261, 701, 890, 896, 1134, 1149
Tru9I TTAA 7 cut(s) 110, 261, 701, 890, 896, 1134, 1149
TscAI CASTG 5 cut(s) 61, 1026, 1305, 1420, 1599
TseI GCWGC 3 cut(s) 926, 1142, 1419
TspDTI ATGAA 6 cut(s) 329, 362, 642, 653, 888, 1641
TspGWI ACGGA 1 cut(s) 1424
TspRI CASTG 5 cut(s) 61, 1026, 1305, 1420, 1599
Van91I CCANNNNNTGG 1 cut(s) 643
XagI CCTNNNNNAGG 1 cut(s) 815
XapI RAATTY 5 cut(s) 173, 483, 1366, 1466, 1483
XbaI TCTAGA 1 cut(s) 581
XceI RCATGY 1 cut(s) 1081
XmnI GAANNNNTTC 1 cut(s) 916
XspI CTAG 3 cut(s) 582, 1007, 1545
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.