AT5G08000

plasmodesmata callose-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008271
Physical Location & Seq
Forward (+)
2563366 .. 2565547
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08000.1

Sequence Viewer

Length: 585 bp
ATGGCTCCTCTGGTTCTCTACCTTCTCACTCTCCTCATGGCTGGCCATACCAGTGCCTCATGGTGTGTGTGCAAAACAGGGCTGAGTGACTCAGTGCTACAAAAGACATTAGACTATGCTTGTGGAAATGGAGCTGACTGTAACCCAACTCACCCAAAAGGCTCTTGCTTCAATCCTGACAATGTTAGGGCTCATTGCAACTATGCAGTCAATAGCTTCTTCCAAAAGAAAGGTCAAGCTTCTGAGTCTTGTAACTTCACTGGTACTGCCACTCTTACCACCACCGATCCCAGCTATACAGGATGTGCATTCCCTTCTAGTGCTAGTGGTTCTAGTGGAAGCGGTAGCACCACCGTGACGCCAGGCAAAAACAGTCCAAAAGGAAGCAACAGCATCACCACATTTCCCGGCGGAAACAGTCCATACTCTGGCACACCATCCACCGGCTTATTAGGAGGCAATATCACTGATGCTACTGGAACCGGGTTGAACCCGGATTACTCAACCGAAAGCAGTGGATTTGCGCTCTATTACTCCAACAACCTTCTGTTAACCGGCTTTTGTTCTCTCGTGATGATGCTCTGA

Protein Analysis

194

Amino Acids

19.9

Weight (kDa)

6.23

Isoelectric Point (pI)

30.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 20 - 91 1.6e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 428
AciI CCGC 2 cut(s) 342, 411
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 2 cut(s) 428, 443
AgsI TTSAA 2 cut(s) 172, 490
AjnI CCWGG 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 4 cut(s) 134, 216, 239, 294
AluI AGCT 4 cut(s) 134, 216, 239, 294
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 217, 249
AspLEI GCGC 1 cut(s) 526
AsuC2I CCSGG 3 cut(s) 408, 484, 494
AsuHPI GGTGA 2 cut(s) 143, 388
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 569
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 363
BcnI CCSGG 3 cut(s) 408, 484, 494
BfaI CTAG 3 cut(s) 318, 324, 333
Bme1390I CCNGG 4 cut(s) 363, 408, 484, 494
BmiI GGNNCC 2 cut(s) 6, 481
BmrFI CCNGG 4 cut(s) 363, 408, 484, 494
BmsI GCATC 3 cut(s) 402, 460, 567
BpuMI CCSGG 3 cut(s) 408, 484, 494
BsaHI GRCGYC 1 cut(s) 359
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 443
Bse118I RCCGGY 2 cut(s) 443, 554
Bse1I ACTGG 3 cut(s) 51, 265, 481
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 363
BseGI GGATG 2 cut(s) 308, 437
BseLI CCNNNNNNNGG 2 cut(s) 428, 443
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 74, 105, 234
BseNI ACTGG 3 cut(s) 51, 265, 481
BseRI GAGGAG 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 290
BshFI GGCC 1 cut(s) 45
BsiSI CCGG 5 cut(s) 408, 444, 483, 494, 555
BslI CCNNNNNNNGG 2 cut(s) 428, 443
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 1 cut(s) 286
BspACI CCGC 2 cut(s) 342, 411
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 3 cut(s) 75, 104, 235
BspLI GGNNCC 2 cut(s) 6, 481
BspPI GGATC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 2 cut(s) 443, 554
BsrI ACTGG 3 cut(s) 51, 265, 481
BssAI RCCGGY 2 cut(s) 443, 554
BssMI GATC 1 cut(s) 286
BssNI GRCGYC 1 cut(s) 359
BssSI CACGAG 1 cut(s) 569
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 4 cut(s) 140, 355, 374, 419
BstACI GRCGYC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 83, 91, 243
BstF5I GGATG 2 cut(s) 308, 437
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 4 cut(s) 361, 406, 482, 492
BsuRI GGCC 1 cut(s) 45
BtsCI GGATG 2 cut(s) 308, 437
BtsI GCAGTG 1 cut(s) 520
BtsIMutI CAGTG 5 cut(s) 58, 99, 258, 465, 520
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 2 cut(s) 443, 554
CseI GACGC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 37, 60
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 3 cut(s) 83, 91, 243
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 43
EciI GGCGGA 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 2 cut(s) 40, 63
FaiI YATR 7 cut(s) 38, 48, 61, 117, 204, 297, 424
FatI CATG 2 cut(s) 36, 59
FokI GGATG 2 cut(s) 315, 424
FriOI GRGCYC 1 cut(s) 193
FspBI CTAG 3 cut(s) 318, 324, 333
GlaI GCGC 1 cut(s) 525
GsaI CCCAGC 1 cut(s) 294
HaeIII GGCC 1 cut(s) 45
HapII CCGG 5 cut(s) 408, 444, 483, 494, 555
HgaI GACGC 1 cut(s) 367
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 2 cut(s) 40, 63
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 2 cut(s) 89, 245
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 5 cut(s) 408, 444, 483, 494, 555
HphI GGTGA 2 cut(s) 143, 388
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 2 cut(s) 244, 584
Hpy188III TCNNGA 2 cut(s) 176, 571
Hpy8I GTNNAC 1 cut(s) 552
HpyAV CCTTC 3 cut(s) 32, 324, 554
HpyCH4III ACNGT 4 cut(s) 140, 355, 374, 419
HpyCH4V TGCA 4 cut(s) 72, 198, 206, 308
HpyF3I CTNAG 3 cut(s) 83, 91, 243
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 2 cut(s) 40, 63
HspAI GCGC 1 cut(s) 524
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 2 cut(s) 10, 131
LweI GCATC 3 cut(s) 402, 460, 567
MaeI CTAG 3 cut(s) 318, 324, 333
MaeIII GTNAC 4 cut(s) 86, 140, 251, 355
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 2 cut(s) 83, 254
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 4 cut(s) 18, 44, 67, 449
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 551
MslI CAYNNNNRTG 2 cut(s) 51, 353
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 5 cut(s) 408, 444, 483, 494, 555
MspR9I CCNGG 4 cut(s) 363, 408, 484, 494
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 363
NciI CCSGG 3 cut(s) 408, 484, 494
NdeII GATC 1 cut(s) 286
NlaIII CATG 2 cut(s) 40, 63
NlaIV GGNNCC 2 cut(s) 6, 481
NmuCI GTSAC 2 cut(s) 86, 355
OliI CACNNNNGTG 1 cut(s) 353
PctI GAATGC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 428
PleI GAGTC 2 cut(s) 83, 253
PpsI GAGTC 2 cut(s) 83, 253
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 6, 481
PsrI GAACNNNNNNTAC 2 cut(s) 482, 514
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 2 cut(s) 51, 353
SaqAI TTAA 1 cut(s) 551
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 2 cut(s) 83, 254
ScrFI CCNGG 4 cut(s) 363, 408, 484, 494
SduI GDGCHC 1 cut(s) 193
SetI ASST 7 cut(s) 24, 136, 218, 235, 241, 296, 546
SfaNI GCATC 3 cut(s) 402, 460, 567
SmiMI CAYNNNNRTG 2 cut(s) 51, 353
SsiI CCGC 2 cut(s) 342, 411
SspMI CTAG 3 cut(s) 318, 324, 333
StyD4I CCNGG 4 cut(s) 361, 406, 482, 492
TaaI ACNGT 4 cut(s) 140, 355, 374, 419
Tru1I TTAA 1 cut(s) 551
Tru9I TTAA 1 cut(s) 551
TscAI CASTG 5 cut(s) 58, 99, 265, 472, 520
TseFI GTSAC 2 cut(s) 86, 355
Tsp45I GTSAC 2 cut(s) 86, 355
TspRI CASTG 5 cut(s) 58, 99, 265, 472, 520
Van91I CCANNNNNTGG 1 cut(s) 428
XspI CTAG 3 cut(s) 318, 324, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.