AT5G10250

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010422
Physical Location & Seq
Reverse (-)
3216881 .. 3219436
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10250.2

Sequence Viewer

Length: 1824 bp
ATGAATTCAGTATCAGCTGCAGAAAGGCTAAAGAGTTTTGGAGATGCTGGTCCAGTATGTAACAAAAGCATTATATTTCCAAGTAGAGTTGTTACATTAGCTAACAGCTTCGAGAAGAAAGACCGTTCCTGGTATGTCAAATCTCAAATCCCTACTGATCTGTCTATTCAAGTCAACGATATTACCTTCAAAGCACACAAGTTTCCTTTGATCTCCAAATGTGGTTACATAAGCTCTATTGAGCTAAAACCTTCAACTTCTGAGAATGGATATCATCTCAAGCTGGAAAACTTCCCAGGTGGAGCAGATACATTCGAGACCATTCTGAAATTTTGCTACAATCTCCCACTGGACTTGAATCCTCTCAATGTAGCTCCACTGAGGTGTGCATCAGAATACTTGTACATGACTGAAGAGTTTGAAGCCGGGAATTTAATCTCCAAGACAGAAGCTTTCATCACTTTTGTAGTACTTGCCTCATGGAGAGATACACTCACTGTTCTGAGATCCTGCACAAACTTATCTCCGTGGGCCGAAAACCTCCAAATCGTAAGGAGATGCTGTGACTTGCTTGCTTGGAAAGCCTGCAATGATAACAACATACCAGAAGATGTTGTAGATAGAAATGAAAGATGCTTATACAATGACATAGCCACCCTTGACATTGATCACTTCATGAGGGTTATAACAACTATGAAAGCAAGACGTGCCAAGCCACAGATTACAGGTAAAATCATCATGAAGTATGCAGATAACTTTTTGCCGGTAATCAATGATGATTTGGAAGGTATAAAAGGTTACGGTTTAGGCAAGAATGAGTTGCAGTTCAGTGTGAACAGAGGAAGAATGGAGGAAAGCAACAGTTTAGGATGTCAAGAACATAAAGAAACTATTGAAAGTTTAGTCAGTGTACTTCCTCCACAATCGGGAGCAGTCTCATGTCACTTTCTCTTAAGGATGCTGAAAACATCAATTGTTTACTCTGCATCACCAGCTTTAATATCTGATCTTGAGAAACGGGTCGGCATGGCTTTGGAAGATGCTAATGTATGTGACCTCCTAATTCCTAACTTCAAAAATGAAGAACAGCAAGAAAGAGTCAGGATTTTTGAGTTTTTCTTGATGCATGAGCAACAACAAGTTCTAGGAAAGCCAAGCATATCCAAGCTCTTGGACAATTATTTAGCCGAGATTGCAAAAGATCCATATCTTCCCATCACAAAGTTTCAGGTTCTAGCAGAAATGTTGCCGGAAAATGCTTGGAAATGTCATGATGGTCTCTATAGAGCAATTGATATGTTTCTCAAGACTCATCCTTCATTGTCAGATCACGATAGAAGAAGATTATGCAAAACAATGAACTGTGAGAAACTATCACTTGATGCATGCCTACACGCTGCACAAAATGATCGGTTGCCTCTAAGAACTATCGTCCAGATCAATACTCAAGTGCTTTTCTCGGAACAAGTAAAGATGAGGATGATGATGCAAGACAAGCTACCAGAGAAGGAGGAAGAAAATTCTGGAGGTCGTGAGGATAAACGAATGTCTAGAGATAATGAAATCATCAAGACACTTAAGGAAGAGTTAGAAAATGTGAAGAAGAAGATGTCAGAGCTTCAGAGCGATTACAATGAGCTTCAACAAGAGTATGAAAGGCTAAGTAGCAAACAGAAGAGCTCACATAACTGGGGATTGCGTTGGCAAAAGGTGAAGAAATCGTTCCAGACAAAACGAGAAGATGAGGAAACAAGAGAGAGGACACGAAGAAGAAGTTCCACGGGACAAAGAACTAGTTTCAGGAGGAGAATGTCAATGTCATAA

Protein Analysis

607

Amino Acids

69.97

Weight (kDa)

8.2

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 213 - 478 1.1e-80 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AclWI GGATC 2 cut(s) 501, 1196
AcsI RAATTY 4 cut(s) 4, 329, 430, 1519
AcuI CTGAAG 2 cut(s) 432, 1604
AfaI GTAC 3 cut(s) 404, 471, 912
AfiI CCNNNNNNNGG 1 cut(s) 926
AflII CTTAAG 2 cut(s) 952, 1577
AgsI TTSAA 8 cut(s) 170, 190, 255, 358, 422, 896, 1075, 1643
AhlI ACTAGT 1 cut(s) 1793
AjiI CACGTC 1 cut(s) 707
AjnI CCWGG 2 cut(s) 128, 295
AleI CACNNNNGTG 1 cut(s) 382
Alw21I GWGCWC 1 cut(s) 1682
Alw26I GTCTC 3 cut(s) 311, 940, 1283
AlwI GGATC 2 cut(s) 501, 1196
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 17, 1397
ApoI RAATTY 4 cut(s) 4, 329, 430, 1519
Asp700I GAANNNNTTC 2 cut(s) 1721, 1774
AspS9I GGNCC 2 cut(s) 50, 531
AsuC2I CCSGG 1 cut(s) 427
AsuHPI GGTGA 2 cut(s) 981, 1723
AvaII GGWCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 1682
Bbv12I GWGCWC 1 cut(s) 1682
BbvI GCAGC 2 cut(s) 4, 1384
BccI CCATC 2 cut(s) 1223, 1268
BciT130I CCWGG 2 cut(s) 130, 297
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 1 cut(s) 427
BcoDI GTCTC 3 cut(s) 311, 940, 1283
BcuI ACTAGT 1 cut(s) 1793
BfaI CTAG 4 cut(s) 1145, 1235, 1551, 1794
BfmI CTRYAG 2 cut(s) 18, 1282
BfrI CTTAAG 2 cut(s) 952, 1577
BisI GCNGC 2 cut(s) 18, 1398
BlsI GCNGC 2 cut(s) 19, 1399
BmcAI AGTACT 1 cut(s) 471
Bme1390I CCNGG 3 cut(s) 130, 297, 427
Bme18I GGWCC 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 2 cut(s) 50, 531
BmrFI CCNGG 3 cut(s) 130, 297, 427
BmrI ACTGGG 1 cut(s) 1699
BmuI ACTGGG 1 cut(s) 1699
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpmI CTGGAG 1 cut(s) 1545
BpuEI CTTGAG 4 cut(s) 263, 1031, 1289, 1431
BpuMI CCSGG 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 1206
BsaI GGTCTC 2 cut(s) 311, 1283
BsaJI CCNNGG 3 cut(s) 295, 527, 1779
BsaXI ACNNNNNCTCC 2 cut(s) 547, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse118I RCCGGY 1 cut(s) 763
Bse1I ACTGG 3 cut(s) 53, 354, 1694
Bse3DI GCAATG 1 cut(s) 595
Bse8I GATNNNNATC 1 cut(s) 1206
BseBI CCWGG 2 cut(s) 130, 297
BseDI CCNNGG 3 cut(s) 295, 527, 1779
BseGI GGATG 4 cut(s) 875, 963, 1312, 1485
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 3 cut(s) 252, 371, 494
BseNI ACTGG 3 cut(s) 53, 354, 1694
BseRI GAGGAG 1 cut(s) 1819
BseXI GCAGC 2 cut(s) 4, 1384
BsgI GTGCAG 2 cut(s) 496, 1383
BshFI GGCC 1 cut(s) 533
BsiHKAI GWGCWC 1 cut(s) 1682
BsiSI CCGG 3 cut(s) 426, 764, 1250
BslFI GGGAC 1 cut(s) 1797
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 3 cut(s) 311, 940, 1283
BsmFI GGGAC 1 cut(s) 1797
BsnI GGCC 1 cut(s) 533
Bso31I GGTCTC 2 cut(s) 311, 1283
Bsp1286I GDGCHC 1 cut(s) 1682
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 3 cut(s) 253, 372, 495
BspHI TCATGA 3 cut(s) 675, 738, 1270
BspMAI CTGCAG 1 cut(s) 22
BspPI GGATC 2 cut(s) 501, 1196
BspQI GCTCTTC 1 cut(s) 1670
BspTI CTTAAG 2 cut(s) 952, 1577
BspTNI GGTCTC 2 cut(s) 311, 1283
BsrDI GCAATG 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 763
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 3 cut(s) 53, 354, 1694
BssAI RCCGGY 1 cut(s) 763
BssECI CCNNGG 3 cut(s) 295, 527, 1779
BssMI GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
Bst2UI CCWGG 2 cut(s) 130, 297
Bst4CI ACNGT 5 cut(s) 125, 499, 803, 863, 1364
Bst6I CTCTTC 3 cut(s) 408, 1578, 1670
BstAFI CTTAAG 2 cut(s) 952, 1577
BstAPI GCANNNNNTGC 1 cut(s) 707
BstAUI TGTACA 1 cut(s) 402
BstC8I GCNNGC 3 cut(s) 573, 586, 1387
BstDEI CTNAG 5 cut(s) 261, 380, 503, 1421, 1661
BstDSI CCRYGG 2 cut(s) 527, 1779
BstF5I GGATG 4 cut(s) 875, 963, 1312, 1485
BstKTI GATC 9 cut(s) 160, 213, 509, 670, 1009, 1204, 1330, 1411, 1440
BstMAI GTCTC 3 cut(s) 311, 940, 1283
BstMBI GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
BstMWI GCNNNNNNNGC 5 cut(s) 581, 707, 992, 1193, 1495
BstNI CCWGG 2 cut(s) 130, 297
BstNSI RCATGY 1 cut(s) 1389
BstSCI CCNGG 3 cut(s) 128, 295, 425
BstSFI CTRYAG 2 cut(s) 18, 1282
BstV1I GCAGC 2 cut(s) 4, 1384
BstX2I RGATCY 2 cut(s) 506, 1201
BstXI CCANNNNNNTGG 1 cut(s) 1171
BstYI RGATCY 2 cut(s) 506, 1201
BsuRI GGCC 1 cut(s) 533
BtgI CCRYGG 2 cut(s) 527, 1779
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 4 cut(s) 875, 963, 1312, 1485
BtsIMutI CAGTG 5 cut(s) 347, 377, 495, 835, 913
Cac8I GCNNGC 3 cut(s) 573, 586, 1387
CciI TCATGA 3 cut(s) 675, 738, 1270
Cfr10I RCCGGY 1 cut(s) 763
Cfr13I GGNCC 2 cut(s) 50, 531
Csp6I GTAC 3 cut(s) 403, 470, 911
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
CviAII CATG 9 cut(s) 406, 480, 676, 739, 939, 1027, 1127, 1271, 1386
CviQI GTAC 3 cut(s) 403, 470, 911
DdeI CTNAG 5 cut(s) 261, 380, 503, 1421, 1661
DpnI GATC 9 cut(s) 159, 212, 508, 669, 1008, 1203, 1329, 1410, 1439
DpnII GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
Eam1104I CTCTTC 3 cut(s) 408, 1578, 1670
EarI CTCTTC 3 cut(s) 408, 1578, 1670
Ecl136II GAGCTC 1 cut(s) 1680
Eco24I GRGCYC 1 cut(s) 1682
Eco31I GGTCTC 2 cut(s) 311, 1283
Eco32I GATATC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 1680
Eco57I CTGAAG 2 cut(s) 432, 1604
EcoICRI GAGCTC 1 cut(s) 1680
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 128, 295
EcoRV GATATC 1 cut(s) 272
EcoT22I ATGCAT 2 cut(s) 1128, 1387
EcoT38I GRGCYC 1 cut(s) 1682
FaeI CATG 9 cut(s) 409, 483, 679, 742, 942, 1030, 1130, 1274, 1389
FaqI GGGAC 1 cut(s) 1797
FatI CATG 9 cut(s) 405, 479, 675, 738, 938, 1026, 1126, 1270, 1385
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 18, 1398
FokI GGATG 4 cut(s) 882, 970, 1299, 1492
FriOI GRGCYC 1 cut(s) 1682
Fsp4HI GCNGC 2 cut(s) 18, 1398
FspBI CTAG 4 cut(s) 1145, 1235, 1551, 1794
GluI GCNGC 2 cut(s) 18, 1398
GsuI CTGGAG 1 cut(s) 1545
HaeIII GGCC 1 cut(s) 533
HapII CCGG 3 cut(s) 426, 764, 1250
Hin1II CATG 9 cut(s) 409, 483, 679, 742, 942, 1030, 1130, 1274, 1389
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 3 cut(s) 358, 1098, 1309
HpaII CCGG 3 cut(s) 426, 764, 1250
HphI GGTGA 2 cut(s) 981, 1723
Hpy166II GTNNAC 4 cut(s) 175, 835, 911, 979
Hpy188I TCNGA 9 cut(s) 262, 327, 394, 504, 1006, 1327, 1462, 1615, 1623
Hpy8I GTNNAC 4 cut(s) 175, 835, 911, 979
HpyAV CCTTC 5 cut(s) 196, 261, 779, 1326, 1501
HpyCH4III ACNGT 5 cut(s) 125, 499, 803, 863, 1364
HpyCH4IV ACGT 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 5 cut(s) 581, 707, 992, 1193, 1495
HpyF3I CTNAG 5 cut(s) 261, 380, 503, 1421, 1661
HpySE526I ACGT 1 cut(s) 706
Hsp92II CATG 9 cut(s) 409, 483, 679, 742, 942, 1030, 1130, 1274, 1389
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
LguI GCTCTTC 1 cut(s) 1670
LmnI GCTCC 3 cut(s) 302, 379, 929
Lsp1109I GCAGC 2 cut(s) 4, 1384
MaeI CTAG 4 cut(s) 1145, 1235, 1551, 1794
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 7 cut(s) 59, 91, 224, 563, 797, 941, 1052
MalI GATC 9 cut(s) 159, 212, 508, 669, 1008, 1203, 1329, 1410, 1439
MboI GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
MfeI CAATTG 2 cut(s) 972, 1290
MflI RGATCY 2 cut(s) 506, 1201
MhlI GDGCHC 1 cut(s) 1682
MluCI AATT 8 cut(s) 4, 329, 430, 972, 1062, 1177, 1290, 1519
MlyI GAGTC 2 cut(s) 1107, 1303
Mph1103I ATGCAT 2 cut(s) 1128, 1387
MroXI GAANNNNTTC 2 cut(s) 1721, 1774
MseI TTAA 4 cut(s) 434, 953, 998, 1578
MslI CAYNNNNRTG 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 17
MspCI CTTAAG 2 cut(s) 952, 1577
MspI CCGG 3 cut(s) 426, 764, 1250
MspR9I CCNGG 3 cut(s) 130, 297, 427
MunI CAATTG 2 cut(s) 972, 1290
MvaI CCWGG 2 cut(s) 130, 297
MwoI GCNNNNNNNGC 5 cut(s) 581, 707, 992, 1193, 1495
NciI CCSGG 1 cut(s) 427
NdeII GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
NlaIII CATG 9 cut(s) 409, 483, 679, 742, 942, 1030, 1130, 1274, 1389
NmeAIII GCCGAG 1 cut(s) 1213
NmuCI GTSAC 3 cut(s) 563, 941, 1052
NsiI ATGCAT 2 cut(s) 1128, 1387
NspI RCATGY 1 cut(s) 1389
OliI CACNNNNGTG 1 cut(s) 382
PaeI GCATGC 1 cut(s) 1389
PagI TCATGA 3 cut(s) 675, 738, 1270
PciSI GCTCTTC 1 cut(s) 1670
PdmI GAANNNNTTC 2 cut(s) 1721, 1774
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 2 cut(s) 19, 1399
PleI GAGTC 2 cut(s) 1106, 1303
PpsI GAGTC 2 cut(s) 1106, 1303
PsiI TTATAA 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 1682
Psp6I CCWGG 2 cut(s) 128, 295
PspGI CCWGG 2 cut(s) 128, 295
PspPI GGNCC 2 cut(s) 50, 531
PstI CTGCAG 1 cut(s) 22
PsuI RGATCY 2 cut(s) 506, 1201
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 404, 471, 912
RsaNI GTAC 3 cut(s) 403, 470, 911
RseI CAYNNNNRTG 1 cut(s) 382
SacI GAGCTC 1 cut(s) 1682
SapI GCTCTTC 1 cut(s) 1670
SaqAI TTAA 4 cut(s) 434, 953, 998, 1578
SatI GCNGC 2 cut(s) 18, 1398
Sau3AI GATC 9 cut(s) 157, 210, 506, 667, 1006, 1201, 1327, 1408, 1437
Sau96I GGNCC 2 cut(s) 50, 531
ScaI AGTACT 1 cut(s) 471
SchI GAGTC 2 cut(s) 1107, 1303
ScrFI CCNGG 3 cut(s) 130, 297, 427
SduI GDGCHC 1 cut(s) 1682
SfcI CTRYAG 2 cut(s) 18, 1282
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 6 cut(s) 278, 952, 1010, 1304, 1446, 1577
SmoI CTYRAG 6 cut(s) 278, 952, 1010, 1304, 1446, 1577
SpeI ACTAGT 1 cut(s) 1793
SphI GCATGC 1 cut(s) 1389
Sse9I AATT 8 cut(s) 4, 329, 430, 972, 1062, 1177, 1290, 1519
SspMI CTAG 4 cut(s) 1145, 1235, 1551, 1794
SstI GAGCTC 1 cut(s) 1682
StyD4I CCNGG 3 cut(s) 128, 295, 425
TaaI ACNGT 5 cut(s) 125, 499, 803, 863, 1364
TaiI ACGT 1 cut(s) 709
TaqI TCGA 2 cut(s) 111, 315
TasI AATT 8 cut(s) 4, 329, 430, 972, 1062, 1177, 1290, 1519
TatI WGTACW 3 cut(s) 402, 469, 910
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 4 cut(s) 434, 953, 998, 1578
Tru9I TTAA 4 cut(s) 434, 953, 998, 1578
TscAI CASTG 5 cut(s) 354, 384, 502, 835, 913
TseFI GTSAC 3 cut(s) 563, 941, 1052
TseI GCWGC 2 cut(s) 17, 1397
Tsp45I GTSAC 3 cut(s) 563, 941, 1052
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 5 cut(s) 354, 384, 502, 835, 913
Vha464I CTTAAG 2 cut(s) 952, 1577
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 4 cut(s) 4, 329, 430, 1519
XbaI TCTAGA 1 cut(s) 1550
XceI RCATGY 1 cut(s) 1389
XmnI GAANNNNTTC 2 cut(s) 1721, 1774
XspI CTAG 4 cut(s) 1145, 1235, 1551, 1794
ZrmI AGTACT 1 cut(s) 471
Zsp2I ATGCAT 2 cut(s) 1128, 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.