Rh2BG256300

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010422
Physical Location & Seq
Forward (+)
26506420 .. 26511882
5463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG256300.1

Sequence Viewer

Length: 1401 bp
ATGAAGAAGTCTCTAACTACAACACAAAATCCAGGCAGTCATTACAATCGCAGTGATCAACTTCAAGACCAAAGCATTGTGATTCCCGCCGCCAAACTCATCAATCTAGCTGATAGCTTCGAAAAGAAAGAACACTCCTGGTTCATCACTTCTCATGTCCCAACGGACCTTACAATTCGAGTTCAAGAAATCACCTTCAATGTTCACAAGTATCCTGTGGTAGCAAAATGTGGCTACATAGGTCAATTGGAATTTCAGCCTTCGATCTCAAATTTTGGCAATGACTTGAAGCTTGAAAACTTCCCAGGTGGACCAGAAACTTTTGAAATGATTCTAAAATTCTGTTATGGTCTCCCACTGGATTTAAACCCTAACAATATAGCTCCACTAAGATGCGGATCAGAATTTCTAGAAATGACTGAAGAACTTCAAGCTGGAAATCTGATTTCCAAGACTGAATCTTTTCTGACATTGGTAGTCCTTTCTTCTTGGAAAGATACCATCACTGTGCTTAAGTCCTGTGAAACTCTATCTCCATGGGCTGAGAATCTTCAAATTGTCAGAAGATGCTGCGACTCAATTGCATGGAAGGCTACTAGAGAAAACACCAGTGGGGATGCCGACGGTCAAGAACAATGGTGGTTTCGTGAAGTGGCCACCTTTCGAATAGATCATTTCATGAGGGTCATATCGACAATAAAAGCAAAGAATACAAGGCCAGAGTTTATAGGTAAATGTATCATGCACTATGCAGAAAGATGGTTGCCAGGCATGGATGTGGAGTTTGAAGGACTAAGAGGATATGGAATTGGAAGAAATGACCTGCAGTTCAGTATCCTGAGTAACAGGAAAGAGGAAGGCGATTATGGACACAGCCAGGAGCAAAAAGCAATTATTGAAAGCCTCCTAAGCGTACTTCCTCCCCAAAATGAAGCTGTTCCATGTAAATTCTTATTGCAGATGTTAAAGATGGCAATGATGTACTCGGCATCGCCAGCTTTGATTTCAGAGCTTGAAAAGAGAGTAGGGATGATGTTAGAAGATGCTACAGTCAATGATTTGCTGATTCCAAGTTATAATATTGCAGACCAAGGGAAACTGGTGAAGTCTCCAGAAGAACGCACGATGCACGACATTGATGTTGTTCAAAGGATTGTGGAATATTTCTTGATGCATGAGCATCAACAGCAAGAGAAGACTGGTAAAACCAATATCAGTAAGCTCTTGGACAGCTACCTGGCTGAGATTGCAGGAGACCCAAATCTCTCGATAACCAAGTTTCAAGTATTGGCTGAATTGCTGCCTGAAAATGCTCGAGCATGTGATGATGGTCTTTATAGGGCTATTGACACCTATCTGAAGGTTACCTACCTACCTGTTCCTATAGTTTTTACTTTATAG

Protein Analysis

466

Amino Acids

52.95

Weight (kDa)

5.31

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 212 - 455 2e-75 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1077
Acc36I ACCTGC 1 cut(s) 831
AciI CCGC 3 cut(s) 87, 90, 396
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 5 cut(s) 251, 271, 338, 404, 947
AcuI CTGAAG 2 cut(s) 441, 1379
AfaI GTAC 2 cut(s) 915, 983
AflII CTTAAG 1 cut(s) 512
AjnI CCWGG 6 cut(s) 31, 137, 304, 766, 876, 1236
Alw26I GTCTC 4 cut(s) 15, 356, 1113, 1248
AlwI GGATC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 2 cut(s) 654, 716
ApeKI GCWGC 2 cut(s) 570, 1300
ApoI RAATTY 5 cut(s) 251, 271, 338, 404, 947
Asp700I GAANNNNTTC 4 cut(s) 330, 426, 462, 936
AspS9I GGNCC 2 cut(s) 166, 311
AsuHPI GGTGA 2 cut(s) 184, 1114
AsuII TTCGAA 2 cut(s) 120, 664
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 2 cut(s) 166, 311
BalI TGGCCA 1 cut(s) 656
BarI GAAGNNNNNNTAC 2 cut(s) 1352, 1384
BbsI GAAGAC 1 cut(s) 1202
BbvI GCAGC 2 cut(s) 557, 1287
BccI CCATC 4 cut(s) 509, 753, 964, 1322
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 6 cut(s) 33, 139, 306, 768, 878, 1238
BciVI GTATCC 2 cut(s) 222, 845
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 4 cut(s) 15, 356, 1113, 1248
BfaI CTAG 3 cut(s) 107, 410, 597
BfmI CTRYAG 3 cut(s) 824, 1047, 1383
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 831
BfuI GTATCC 2 cut(s) 222, 845
BisI GCNGC 3 cut(s) 90, 571, 1301
BlsI GCNGC 3 cut(s) 91, 572, 1302
Bme1390I CCNGG 6 cut(s) 33, 139, 306, 768, 878, 1238
Bme18I GGWCC 2 cut(s) 166, 311
BmeT110I CYCGRG 1 cut(s) 1314
BmgT120I GGNCC 2 cut(s) 166, 311
BmrFI CCNGG 6 cut(s) 33, 139, 306, 768, 878, 1238
BmsI GCATC 8 cut(s) 383, 557, 607, 998, 1033, 1116, 1161, 1189
BpiI GAAGAC 1 cut(s) 1202
BpmI CTGGAG 1 cut(s) 1095
Bpu10I CCTNAGC 1 cut(s) 908
Bpu14I TTCGAA 2 cut(s) 120, 664
BsaBI GATNNNNATC 1 cut(s) 397
BsaI GGTCTC 2 cut(s) 356, 1248
BsaJI CCNNGG 3 cut(s) 304, 536, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bse1I ACTGG 4 cut(s) 363, 609, 1104, 1204
Bse3DI GCAATG 2 cut(s) 286, 981
Bse8I GATNNNNATC 1 cut(s) 397
BseBI CCWGG 6 cut(s) 33, 139, 306, 768, 878, 1238
BseDI CCNNGG 3 cut(s) 304, 536, 1090
BseGI GGATG 3 cut(s) 622, 781, 1035
BseJI GATNNNNATC 1 cut(s) 397
BseMI GCAATG 2 cut(s) 286, 981
BseMII CTCAG 3 cut(s) 534, 830, 1233
BseNI ACTGG 4 cut(s) 363, 609, 1104, 1204
BseXI GCAGC 2 cut(s) 557, 1287
BshFI GGCC 2 cut(s) 656, 718
BsiHKCI CYCGRG 1 cut(s) 1314
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 4 cut(s) 15, 356, 1113, 1248
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 2 cut(s) 656, 718
Bso31I GGTCTC 2 cut(s) 356, 1248
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 2 cut(s) 120, 664
Bsp143I GATC 4 cut(s) 55, 264, 398, 670
Bsp19I CCATGG 1 cut(s) 536
BspACI CCGC 3 cut(s) 87, 90, 396
BspANI GGCC 2 cut(s) 656, 718
BspCNI CTCAG 3 cut(s) 535, 831, 1234
BspHI TCATGA 1 cut(s) 678
BspMAI CTGCAG 1 cut(s) 828
BspMI ACCTGC 1 cut(s) 831
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 2 cut(s) 120, 664
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 2 cut(s) 356, 1248
BsrDI GCAATG 2 cut(s) 286, 981
BsrI ACTGG 4 cut(s) 363, 609, 1104, 1204
BssECI CCNNGG 3 cut(s) 304, 536, 1090
BssMI GATC 4 cut(s) 55, 264, 398, 670
BssT1I CCWWGG 2 cut(s) 536, 1090
Bst2UI CCWGG 6 cut(s) 33, 139, 306, 768, 878, 1238
Bst4CI ACNGT 3 cut(s) 508, 626, 1051
BstAFI CTTAAG 1 cut(s) 512
BstBI TTCGAA 2 cut(s) 120, 664
BstC8I GCNNGC 1 cut(s) 996
BstDEI CTNAG 6 cut(s) 389, 543, 794, 839, 908, 1242
BstDSI CCRYGG 1 cut(s) 536
BstEII GGTNACC 1 cut(s) 1363
BstF5I GGATG 3 cut(s) 622, 781, 1035
BstKTI GATC 4 cut(s) 58, 267, 401, 673
BstMAI GTCTC 4 cut(s) 15, 356, 1113, 1248
BstMBI GATC 4 cut(s) 55, 264, 398, 670
BstMWI GCNNNNNNNGC 5 cut(s) 590, 909, 995, 1186, 1247
BstNI CCWGG 6 cut(s) 33, 139, 306, 768, 878, 1238
BstNSI RCATGY 1 cut(s) 1323
BstPI GGTNACC 1 cut(s) 1363
BstSCI CCNGG 6 cut(s) 31, 137, 304, 766, 876, 1236
BstSFI CTRYAG 3 cut(s) 824, 1047, 1383
BstV1I GCAGC 2 cut(s) 557, 1287
BstV2I GAAGAC 1 cut(s) 1202
BsuI GTATCC 2 cut(s) 222, 845
BsuRI GGCC 2 cut(s) 656, 718
BtgI CCRYGG 1 cut(s) 536
BtgZI GCGATG 1 cut(s) 975
BtsCI GGATG 3 cut(s) 622, 781, 1035
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 4 cut(s) 58, 356, 504, 616
BveI ACCTGC 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 996
CciI TCATGA 1 cut(s) 678
Cfr13I GGNCC 2 cut(s) 166, 311
Csp6I GTAC 2 cut(s) 914, 982
CviAII CATG 9 cut(s) 155, 537, 585, 679, 742, 772, 942, 1175, 1320
CviQI GTAC 2 cut(s) 914, 982
DdeI CTNAG 6 cut(s) 389, 543, 794, 839, 908, 1242
DpnI GATC 4 cut(s) 57, 266, 400, 672
DpnII GATC 4 cut(s) 55, 264, 398, 670
DraI TTTAAA 1 cut(s) 366
EaeI YGGCCR 1 cut(s) 654
Eco130I CCWWGG 2 cut(s) 536, 1090
Eco31I GGTCTC 2 cut(s) 356, 1248
Eco47I GGWCC 2 cut(s) 166, 311
Eco57I CTGAAG 2 cut(s) 441, 1379
Eco88I CYCGRG 1 cut(s) 1314
Eco91I GGTNACC 1 cut(s) 1363
EcoO65I GGTNACC 1 cut(s) 1363
EcoRII CCWGG 6 cut(s) 31, 137, 304, 766, 876, 1236
EcoT14I CCWWGG 2 cut(s) 536, 1090
EcoT22I ATGCAT 1 cut(s) 1176
ErhI CCWWGG 2 cut(s) 536, 1090
FaeI CATG 9 cut(s) 158, 540, 588, 682, 745, 775, 945, 1178, 1323
FaqI GGGAC 1 cut(s) 143
FatI CATG 9 cut(s) 154, 536, 584, 678, 741, 771, 941, 1174, 1319
FauI CCCGC 1 cut(s) 94
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 3 cut(s) 90, 571, 1301
FokI GGATG 3 cut(s) 629, 788, 1042
Fsp4HI GCNGC 3 cut(s) 90, 571, 1301
FspBI CTAG 3 cut(s) 107, 410, 597
GluI GCNGC 3 cut(s) 90, 571, 1301
GsuI CTGGAG 1 cut(s) 1095
HaeIII GGCC 2 cut(s) 656, 718
Hin1II CATG 9 cut(s) 158, 540, 588, 682, 745, 775, 945, 1178, 1323
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 82, 331, 458, 547, 575, 1066
HphI GGTGA 2 cut(s) 184, 1114
Hpy166II GTNNAC 2 cut(s) 205, 311
Hpy188I TCNGA 6 cut(s) 403, 444, 468, 563, 1009, 1359
Hpy8I GTNNAC 2 cut(s) 205, 311
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 6 cut(s) 205, 270, 583, 782, 851, 1354
HpyCH4III ACNGT 3 cut(s) 508, 626, 1051
HpyCH4V TGCA 9 cut(s) 584, 745, 752, 826, 958, 1085, 1129, 1174, 1250
HpyF10VI GCNNNNNNNGC 5 cut(s) 590, 909, 995, 1186, 1247
HpyF3I CTNAG 6 cut(s) 389, 543, 794, 839, 908, 1242
Hsp92II CATG 9 cut(s) 158, 540, 588, 682, 745, 775, 945, 1178, 1323
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 4 cut(s) 55, 264, 398, 670
LmnI GCTCC 2 cut(s) 388, 880
Lsp1109I GCAGC 2 cut(s) 557, 1287
LweI GCATC 8 cut(s) 383, 557, 607, 998, 1033, 1116, 1161, 1189
MaeI CTAG 3 cut(s) 107, 410, 597
MaeIII GTNAC 2 cut(s) 842, 1363
MalI GATC 4 cut(s) 57, 266, 400, 672
MboI GATC 4 cut(s) 55, 264, 398, 670
MboII GAAGA 9 cut(s) 16, 434, 477, 542, 576, 825, 1052, 1127, 1207
MfeI CAATTG 2 cut(s) 245, 579
MlsI TGGCCA 1 cut(s) 656
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 569
MnlI CCTC 5 cut(s) 675, 791, 847, 914, 930
Mox20I TGGCCA 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 1176
MroXI GAANNNNTTC 4 cut(s) 330, 426, 462, 936
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 365, 513, 965
MslI CAYNNNNRTG 3 cut(s) 391, 506, 776
Msp20I TGGCCA 1 cut(s) 656
MspCI CTTAAG 1 cut(s) 512
MspR9I CCNGG 6 cut(s) 33, 139, 306, 768, 878, 1238
MunI CAATTG 2 cut(s) 245, 579
MvaI CCWGG 6 cut(s) 33, 139, 306, 768, 878, 1238
MwoI GCNNNNNNNGC 5 cut(s) 590, 909, 995, 1186, 1247
NcoI CCATGG 1 cut(s) 536
NdeII GATC 4 cut(s) 55, 264, 398, 670
NlaIII CATG 9 cut(s) 158, 540, 588, 682, 745, 775, 945, 1178, 1323
NmeAIII GCCGAG 1 cut(s) 965
NsiI ATGCAT 1 cut(s) 1176
NspI RCATGY 1 cut(s) 1323
NspV TTCGAA 2 cut(s) 120, 664
PaeR7I CTCGAG 1 cut(s) 1314
PagI TCATGA 1 cut(s) 678
PdmI GAANNNNTTC 4 cut(s) 330, 426, 462, 936
PfeI GAWTC 5 cut(s) 82, 331, 458, 547, 1066
PkrI GCNGC 3 cut(s) 91, 572, 1302
PleI GAGTC 1 cut(s) 569
PpsI GAGTC 1 cut(s) 569
PsiI TTATAA 1 cut(s) 1077
Psp6I CCWGG 6 cut(s) 31, 137, 304, 766, 876, 1236
PspEI GGTNACC 1 cut(s) 1363
PspGI CCWGG 6 cut(s) 31, 137, 304, 766, 876, 1236
PspPI GGNCC 2 cut(s) 166, 311
PspXI VCTCGAGB 1 cut(s) 1314
PstI CTGCAG 1 cut(s) 828
RsaI GTAC 2 cut(s) 915, 983
RsaNI GTAC 2 cut(s) 914, 982
RseI CAYNNNNRTG 3 cut(s) 391, 506, 776
SaqAI TTAA 3 cut(s) 365, 513, 965
SatI GCNGC 3 cut(s) 90, 571, 1301
Sau3AI GATC 4 cut(s) 55, 264, 398, 670
Sau96I GGNCC 2 cut(s) 166, 311
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 6 cut(s) 33, 139, 306, 768, 878, 1238
SfaNI GCATC 8 cut(s) 383, 557, 607, 998, 1033, 1116, 1161, 1189
SfcI CTRYAG 3 cut(s) 824, 1047, 1383
Sfr274I CTCGAG 1 cut(s) 1314
SfuI TTCGAA 2 cut(s) 120, 664
SinI GGWCC 2 cut(s) 166, 311
SlaI CTCGAG 1 cut(s) 1314
SmiMI CAYNNNNRTG 3 cut(s) 391, 506, 776
SmlI CTYRAG 2 cut(s) 512, 1314
SmoI CTYRAG 2 cut(s) 512, 1314
SsiI CCGC 3 cut(s) 87, 90, 396
SspI AATATT 2 cut(s) 1081, 1163
SspMI CTAG 3 cut(s) 107, 410, 597
StyD4I CCNGG 6 cut(s) 31, 137, 304, 766, 876, 1236
StyI CCWWGG 2 cut(s) 536, 1090
TaaI ACNGT 3 cut(s) 508, 626, 1051
TaqI TCGA 7 cut(s) 120, 178, 263, 664, 692, 1268, 1315
TatI WGTACW 1 cut(s) 981
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 5 cut(s) 82, 331, 458, 547, 1066
Tru1I TTAA 3 cut(s) 365, 513, 965
Tru9I TTAA 3 cut(s) 365, 513, 965
TscAI CASTG 4 cut(s) 58, 363, 511, 616
TseI GCWGC 2 cut(s) 570, 1300
TspDTI ATGAA 4 cut(s) 17, 133, 667, 945
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 4 cut(s) 58, 363, 511, 616
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 166, 311
XapI RAATTY 5 cut(s) 251, 271, 338, 404, 947
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 1 cut(s) 1323
XhoI CTCGAG 1 cut(s) 1314
XmnI GAANNNNTTC 4 cut(s) 330, 426, 462, 936
XspI CTAG 3 cut(s) 107, 410, 597
Zsp2I ATGCAT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.