RLG00000018068

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010422
Physical Location & Seq
Forward (+)
25023754 .. 25027760
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018068

Sequence Viewer

Length: 1857 bp
ATGAAGAAGTCTCTAACTACAACACAAAATCCAGGCAGTCATTACAATCGCAGTGATCAACTTCAGGACCAAAGCATTGTGATTCCCGCCAAATTCATCAATCTAGCTGATAGCTTCGAAAAGACAGAACACTCCTGGTTCATCACTTCTCATGTCCCAACGGACATTACAATTCGAGTTCAAGAAATTACCTTTAATGTTCACAAGTATCCTTTGGTAGCAAAATGTGGCTATATAGGTCAATTGGAATTTCAGCCTTCGATCTCAAATTTTGGCAATGACTTGAAGCTTGAAAACTTCCCAGGTGGACCAGAAACCTTTGAAATGATTCTAAAATTCTGTTATGGTCTCCCACTGGACTTAAACCCAAACAATATAGCTCCGCTAAGATGCGCGTCAGAATTTCTAGAAATGACTGAAGAAATTCAAGCTGGAAATCTGATTTCCAAGACTGAAGCTTTTCTGACATTGGTAGTCCTTTCTTCTTGGAAAGATACCATCACTGTACTGAAGTCCTGTGAAACTCTATCTCCATGGGCTGAGAATCTTCAAATTGTCAGAAGATGCTGCGACTCAATTGCATGGAAGGCTACTAGAGAAAACACCAGTGGGGATGCCGACAGTCAAGAACAATGGTGGTTTCGTGAAGTGGCCACCTTCCGAATAGATCATTTCATGAGGGTCATATCGACAATAAAGGCAAAGAATACAAGGCCAGAGTTTATAGGTAAATGTATTATGCACTATGCAGAAAGATGGTTGCCAGGCATGGATATGGAGTTTGAAGGACTAAGAGGATATGGAATTGGAAGAAATGACCTGCAGTTCAGTATCCTGAGTAACAGGAAAGAGGAAGGCGGTTATGGACACAGCCAGGAGCAAAAAGCAATTATTGAAAGCCTCCTAAGCGTACTTCCTCCCCAAAATGAAGCTGTTCCATGTAAATTCTTATTGCAGATGTTAAAGATGGCAATGATGTACTCGGCATCGCCAGCTTTGACTTCAGAGCTTGAAAAGAGAGTAGGGATGATGTTAGAAGATGCCACTGTCGATGATTTGCTGATTCCAAGTTATAATATTGCAGACCAAGGGAAACTGGTGAAGTCATCAGAAGAACGCACAATGCACGACATTGATGTTGTTCAAAGGATTGTGGAATATTTCTTGATGCATGAGCATCAACAGCAAGAGAAGACTGGTAAAACCAATATCAGTAAGCTATTGGACAGCTACCTGGCTGAGATTGCAGGAGACCCAAATCTCTCGATAACCAAGTTTCAAGTATTGGCTGAATTGCTGCCTGAAAATGCTCGAGCATGTGATGATGGTCTTTATAGGGCTGTTGACACCTATCTGAAGACTCATCCTTCACTGCCCGAGCATGATCGAAGAAGGCTATGCAGGATAATGAACTGTGAGAAACTATCACTTGATGCATGCGTGCATGCTGCACAAAATGATCGACTGCCTATGAAAACCATTGTCCAGGTTCTGTTCTCAGAGCAAGTAAAGATGAGGACAGCAATGCAAGAGAAGGAATCAGCACCAACTGGCAATAACTCTGAACAGGAAGGAAACCGCTCATCTACAGATGCAGAAATTAAGAATCTTAAAGCGGAACTTGAGAATGTGAAGATAAGGATGGCAGAGCTGCAAAAAGACTACTCAGAGCTGCAACAGGAATATGAAAAGATAAACAACAAGCAAAAGATTGTGTCAAGTTGGAGCTCGGGATGGAGAAAAATCAAGAAATCTTTCCATACAAAATCTGAAGCGAATGAAACTGGGGAAGGACAGCAAAGAACCAACTCAATTGGGGGCAGAATCAGCTCCAGACGAAGGTCATCCATGTCCTGA

Protein Analysis

619

Amino Acids

70.43

Weight (kDa)

6.04

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 211 - 480 1.9e-91 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1074
Acc36I ACCTGC 1 cut(s) 828
AccBSI CCGCTC 1 cut(s) 1581
AccII CGCG 1 cut(s) 395
AciI CCGC 5 cut(s) 87, 383, 858, 1579, 1616
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 7 cut(s) 92, 248, 268, 335, 401, 423, 944
AcuI CTGAAG 7 cut(s) 47, 438, 474, 530, 987, 1376, 1791
AfaI GTAC 3 cut(s) 507, 912, 980
AfiI CCNNNNNNNGG 1 cut(s) 1839
AjnI CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
Alw21I GWGCWC 1 cut(s) 1730
Alw26I GTCTC 3 cut(s) 15, 353, 1245
AlwNI CAGNNNCTG 1 cut(s) 1492
Ama87I CYCGRG 3 cut(s) 1311, 1376, 1729
AoxI GGCC 2 cut(s) 651, 713
ApeKI GCWGC 5 cut(s) 567, 1297, 1448, 1651, 1672
ApoI RAATTY 7 cut(s) 92, 248, 268, 335, 401, 423, 944
Asp700I GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 67, 308
AsuHPI GGTGA 1 cut(s) 1111
AsuII TTCGAA 1 cut(s) 117
AvaI CYCGRG 3 cut(s) 1311, 1376, 1729
AvaII GGWCC 2 cut(s) 67, 308
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 1 cut(s) 1730
BbsI GAAGAC 2 cut(s) 1199, 1364
Bbv12I GWGCWC 1 cut(s) 1730
BbvI GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BccI CCATC 6 cut(s) 506, 750, 961, 1319, 1636, 1728
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BciVI GTATCC 2 cut(s) 219, 842
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 3 cut(s) 15, 353, 1245
BfaI CTAG 3 cut(s) 104, 407, 594
BfmI CTRYAG 2 cut(s) 821, 1587
BfuAI ACCTGC 1 cut(s) 828
BfuI GTATCC 2 cut(s) 219, 842
BisI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
BlsI GCNGC 5 cut(s) 569, 1299, 1450, 1653, 1674
Bme1390I CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
Bme18I GGWCC 2 cut(s) 67, 308
BmeT110I CYCGRG 3 cut(s) 1311, 1376, 1729
BmgT120I GGNCC 2 cut(s) 67, 308
BmrFI CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BmrI ACTGGG 1 cut(s) 1794
BmsI GCATC 9 cut(s) 380, 554, 604, 995, 1030, 1158, 1186, 1423, 1582
BmuI ACTGGG 1 cut(s) 1794
BoxI GACNNNNGTC 1 cut(s) 1840
BpiI GAAGAC 2 cut(s) 1199, 1364
BpmI CTGGAG 1 cut(s) 1816
Bpu10I CCTNAGC 1 cut(s) 905
Bpu14I TTCGAA 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 1643
BsaI GGTCTC 2 cut(s) 353, 1245
BsaJI CCNNGG 3 cut(s) 301, 533, 1087
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 1839
Bse1I ACTGG 6 cut(s) 360, 606, 1101, 1201, 1555, 1789
Bse3DI GCAATG 3 cut(s) 283, 978, 1530
BseBI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BseDI CCNNGG 3 cut(s) 301, 533, 1087
BseGI GGATG 6 cut(s) 619, 1032, 1363, 1647, 1739, 1844
BseLI CCNNNNNNNGG 1 cut(s) 1839
BseMI GCAATG 3 cut(s) 283, 978, 1530
BseMII CTCAG 5 cut(s) 531, 827, 1230, 1512, 1680
BseNI ACTGG 6 cut(s) 360, 606, 1101, 1201, 1555, 1789
BseXI GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BsgI GTGCAG 1 cut(s) 1434
Bsh1236I CGCG 1 cut(s) 395
BshFI GGCC 2 cut(s) 653, 715
BsiHKAI GWGCWC 1 cut(s) 1730
BsiHKCI CYCGRG 3 cut(s) 1311, 1376, 1729
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 1839
BsmAI GTCTC 3 cut(s) 15, 353, 1245
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 653, 715
Bso31I GGTCTC 2 cut(s) 353, 1245
BsoBI CYCGRG 3 cut(s) 1311, 1376, 1729
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 1730
Bsp143I GATC 5 cut(s) 55, 261, 667, 1384, 1459
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 5 cut(s) 87, 383, 858, 1579, 1616
BspANI GGCC 2 cut(s) 653, 715
BspCNI CTCAG 5 cut(s) 532, 828, 1231, 1511, 1679
BspFNI CGCG 1 cut(s) 395
BspHI TCATGA 1 cut(s) 675
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 117
BspTNI GGTCTC 2 cut(s) 353, 1245
BsrBI CCGCTC 1 cut(s) 1581
BsrDI GCAATG 3 cut(s) 283, 978, 1530
BsrI ACTGG 6 cut(s) 360, 606, 1101, 1201, 1555, 1789
BssECI CCNNGG 3 cut(s) 301, 533, 1087
BssMI GATC 5 cut(s) 55, 261, 667, 1384, 1459
BssT1I CCWWGG 2 cut(s) 533, 1087
Bst2UI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
Bst4CI ACNGT 4 cut(s) 505, 623, 1048, 1415
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 993, 1438, 1442, 1446
BstDEI CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 6 cut(s) 619, 1032, 1363, 1647, 1739, 1844
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 5 cut(s) 58, 264, 670, 1387, 1462
BstMAI GTCTC 3 cut(s) 15, 353, 1245
BstMBI GATC 5 cut(s) 55, 261, 667, 1384, 1459
BstMWI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
BstNI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BstNSI RCATGY 3 cut(s) 1320, 1440, 1448
BstPAI GACNNNNGTC 1 cut(s) 1840
BstSCI CCNGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
BstSFI CTRYAG 2 cut(s) 821, 1587
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BstV2I GAAGAC 2 cut(s) 1199, 1364
BsuI GTATCC 2 cut(s) 219, 842
BsuRI GGCC 2 cut(s) 653, 715
BtgI CCRYGG 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 972
BtsCI GGATG 6 cut(s) 619, 1032, 1363, 1647, 1739, 1844
BtsI GCAGTG 2 cut(s) 58, 1370
BtsIMutI CAGTG 6 cut(s) 58, 353, 501, 613, 1044, 1370
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 4 cut(s) 993, 1438, 1442, 1446
CaiI CAGNNNCTG 1 cut(s) 1492
CciI TCATGA 1 cut(s) 675
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 67, 308
CseI GACGC 1 cut(s) 384
Csp6I GTAC 3 cut(s) 506, 911, 979
CviQI GTAC 3 cut(s) 506, 911, 979
DdeI CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
DpnI GATC 5 cut(s) 57, 263, 669, 1386, 1461
DpnII GATC 5 cut(s) 55, 261, 667, 1384, 1459
EaeI YGGCCR 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 1728
Eco130I CCWWGG 2 cut(s) 533, 1087
Eco24I GRGCYC 1 cut(s) 1730
Eco31I GGTCTC 2 cut(s) 353, 1245
Eco47I GGWCC 2 cut(s) 67, 308
Eco53kI GAGCTC 1 cut(s) 1728
Eco57I CTGAAG 7 cut(s) 47, 438, 474, 530, 987, 1376, 1791
Eco88I CYCGRG 3 cut(s) 1311, 1376, 1729
EcoICRI GAGCTC 1 cut(s) 1728
EcoRII CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
EcoT14I CCWWGG 2 cut(s) 533, 1087
EcoT22I ATGCAT 2 cut(s) 1173, 1438
EcoT38I GRGCYC 1 cut(s) 1730
ErhI CCWWGG 2 cut(s) 533, 1087
FalI AAGNNNNNCTT 2 cut(s) 1605, 1637
FaqI GGGAC 1 cut(s) 140
FauI CCCGC 1 cut(s) 94
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
FokI GGATG 6 cut(s) 626, 1039, 1350, 1654, 1746, 1831
FriOI GRGCYC 1 cut(s) 1730
Fsp4HI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
FspBI CTAG 3 cut(s) 104, 407, 594
GlaI GCGC 1 cut(s) 394
GluI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
GsuI CTGGAG 1 cut(s) 1816
HaeIII GGCC 2 cut(s) 653, 715
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 395
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 1 cut(s) 1345
HindII GTYRAC 1 cut(s) 1345
HindIII AAGCTT 2 cut(s) 287, 456
HinfI GANTC 9 cut(s) 82, 328, 544, 572, 1063, 1360, 1538, 1606, 1824
HphI GGTGA 1 cut(s) 1111
Hpy166II GTNNAC 3 cut(s) 202, 308, 1345
Hpy8I GTNNAC 3 cut(s) 202, 308, 1345
HpyCH4III ACNGT 4 cut(s) 505, 623, 1048, 1415
HpyF10VI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
HpyF3I CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
HspAI GCGC 1 cut(s) 393
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 5 cut(s) 55, 261, 667, 1384, 1459
LmnI GCTCC 4 cut(s) 385, 877, 1725, 1835
Lsp1109I GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
LweI GCATC 9 cut(s) 380, 554, 604, 995, 1030, 1158, 1186, 1423, 1582
MaeI CTAG 3 cut(s) 104, 407, 594
MaeIII GTNAC 1 cut(s) 839
MalI GATC 5 cut(s) 57, 263, 669, 1386, 1461
MbiI CCGCTC 1 cut(s) 1581
MboI GATC 5 cut(s) 55, 261, 667, 1384, 1459
MfeI CAATTG 3 cut(s) 242, 576, 1812
MhlI GDGCHC 1 cut(s) 1730
MlsI TGGCCA 1 cut(s) 653
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 2 cut(s) 566, 1354
MmeI TCCRAC 1 cut(s) 1703
MnlI CCTC 6 cut(s) 672, 788, 844, 911, 927, 1509
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 2 cut(s) 1173, 1438
MroXI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
MscI TGGCCA 1 cut(s) 653
MseI TTAA 5 cut(s) 195, 362, 962, 1602, 1611
MslI CAYNNNNRTG 1 cut(s) 773
Msp20I TGGCCA 1 cut(s) 653
MspR9I CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
MunI CAATTG 3 cut(s) 242, 576, 1812
MvaI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
NcoI CCATGG 1 cut(s) 533
NdeII GATC 5 cut(s) 55, 261, 667, 1384, 1459
NmeAIII GCCGAG 1 cut(s) 962
NsiI ATGCAT 2 cut(s) 1173, 1438
NspI RCATGY 3 cut(s) 1320, 1440, 1448
NspV TTCGAA 1 cut(s) 117
PaeI GCATGC 2 cut(s) 1440, 1448
PaeR7I CTCGAG 1 cut(s) 1311
PagI TCATGA 1 cut(s) 675
PdmI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
PfeI GAWTC 7 cut(s) 82, 328, 544, 1063, 1538, 1606, 1824
PkrI GCNGC 5 cut(s) 569, 1299, 1450, 1653, 1674
PleI GAGTC 2 cut(s) 566, 1354
PpsI GAGTC 2 cut(s) 566, 1354
PshAI GACNNNNGTC 1 cut(s) 1840
PsiI TTATAA 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 1730
Psp6I CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
PspGI CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
PspPI GGNCC 2 cut(s) 67, 308
PspXI VCTCGAGB 1 cut(s) 1311
PstI CTGCAG 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 1492
RsaI GTAC 3 cut(s) 507, 912, 980
RsaNI GTAC 3 cut(s) 506, 911, 979
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 1 cut(s) 1730
SaqAI TTAA 5 cut(s) 195, 362, 962, 1602, 1611
SatI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
Sau3AI GATC 5 cut(s) 55, 261, 667, 1384, 1459
Sau96I GGNCC 2 cut(s) 67, 308
SchI GAGTC 2 cut(s) 566, 1354
ScrFI CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
SduI GDGCHC 1 cut(s) 1730
SfaNI GCATC 9 cut(s) 380, 554, 604, 995, 1030, 1158, 1186, 1423, 1582
SfcI CTRYAG 2 cut(s) 821, 1587
Sfr274I CTCGAG 1 cut(s) 1311
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 2 cut(s) 67, 308
SlaI CTCGAG 1 cut(s) 1311
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 2 cut(s) 1311, 1622
SmoI CTYRAG 2 cut(s) 1311, 1622
SphI GCATGC 2 cut(s) 1440, 1448
SsiI CCGC 5 cut(s) 87, 383, 858, 1579, 1616
SspI AATATT 2 cut(s) 1078, 1160
SspMI CTAG 3 cut(s) 104, 407, 594
SstI GAGCTC 1 cut(s) 1730
StyD4I CCNGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
StyI CCWWGG 2 cut(s) 533, 1087
TaaI ACNGT 4 cut(s) 505, 623, 1048, 1415
TaqI TCGA 9 cut(s) 117, 175, 260, 689, 1050, 1265, 1312, 1387, 1462
TatI WGTACW 2 cut(s) 505, 978
TfiI GAWTC 7 cut(s) 82, 328, 544, 1063, 1538, 1606, 1824
Tru1I TTAA 5 cut(s) 195, 362, 962, 1602, 1611
Tru9I TTAA 5 cut(s) 195, 362, 962, 1602, 1611
TscAI CASTG 6 cut(s) 58, 360, 508, 613, 1051, 1377
TseI GCWGC 5 cut(s) 567, 1297, 1448, 1651, 1672
TspDTI ATGAA 9 cut(s) 17, 85, 130, 664, 942, 1424, 1487, 1701, 1794
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 6 cut(s) 58, 360, 508, 613, 1051, 1377
VpaK11BI GGWCC 2 cut(s) 67, 308
XapI RAATTY 7 cut(s) 92, 248, 268, 335, 401, 423, 944
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 3 cut(s) 1320, 1440, 1448
XhoI CTCGAG 1 cut(s) 1311
XmnI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
XspI CTAG 3 cut(s) 104, 407, 594
Zsp2I ATGCAT 2 cut(s) 1173, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.