MD01G1030300.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010422
Physical Location & Seq
Reverse (-)
10789272 .. 10790679
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1030300.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTTATTCCATCCAAATCTCTCATCTGCCACTCCCTACACATCCGGCCAAAAGATGAACAAGTCCCTGAATCCACCGCAAATTCCAGAGGCCGACCACGAGGGCACTAAGCGCGTCCTAGTTCCTTCAAAACACATCACTTTAGCCGCCACCTTCGAACAGAAACAACACTCCTGGTTTATCGATTCTCATGTACCAACCGACCTAACCATTCAAGTTGAAGGCGTCGCCTTTAACGTTCACAAGTATCCCTTGCTATCAAAATGTGGCTACATAGGTCGACTGGAATTTCATCCTTCACATTCAAACTTCGGTTATGAGCTCGAGCTTCAAAACTTCCCCGGTGGATCAGAAACCTTTGAACTCATTTTAAAATTCTGTTACGGTCTTCCAGTGGGCTTAAACCCTAACAATATAGCTCCGCTAAGATGCGCCTCGGAATTTCTTGAAATGACTGAAGAAATTCAAGATGGAAATCTCATTTCTAAGACTGAATCTTTCCTCACACTTGTAGTCCTTTCTTCATGGAAAGACACCGTTTCTGTGCTCAAATCTTGTGAAACTCTATCTCCATGGGCTGAAAATCTTCAAATTGTCAGACGCTGCTGCGACACAATTGCTTGGAAGGCTTCCAGAGAGAACGTAATTGGGGATGCAGATGGTCAAGAAGGCTGGTGGTTTAACGAAGTGGCTACCTTTCGAATAGATCATTTCATGAGGACTATAACAGCAATAAAGGCAAAGAGTACCAGACCAGCGTTTGTAGGTAAATGTATTATGCGCTATGCAGAAAGATGGTTGACAGGCATGGATGTGGAGTTTGAAGGACTAAGAGGATATGAACTTGGAAAGAATGACCTGCGGTTCAGTATATTGAGTAGGAGGAAGGAAGACGGGGGTTACGAATATATCAAGGAGCAAAAAGCAATTATTGAAAGCCTAGTAAGCATCCTACCTCCTCAAAACGAGGCTCTTCCTTGTAAGTTCTTATTGCAGATGTTAAAGTTGGCCATGCTGTACTCTGCATCGCCAGCGTTGATTTCAGAGCTTGAAAAAAGAGCAGGGATGATGTTGAAAGATGCCAATGTGAACGATCTGCTGATTCCTAGTTACTATAATGCAGATCAAGGGAAACTGGCTAAGTGA

Protein Analysis

382

Amino Acids

43.05

Weight (kDa)

6.18

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 225 - 373 4.5e-35 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 867
AccI GTMKAC 1 cut(s) 280
AccII CGCG 1 cut(s) 114
AciI CCGC 4 cut(s) 77, 147, 422, 862
AclI AACGTT 1 cut(s) 237
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 2 cut(s) 46, 1008
AcsI RAATTY 5 cut(s) 81, 287, 374, 440, 462
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 3 cut(s) 195, 748, 1019
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 4 cut(s) 322, 328, 419, 1048
AluI AGCT 4 cut(s) 322, 328, 419, 1048
Alw21I GWGCWC 2 cut(s) 324, 549
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 603
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 3 cut(s) 46, 90, 1008
ApeKI GCWGC 2 cut(s) 603, 606
ApoI RAATTY 5 cut(s) 81, 287, 374, 440, 462
Asp700I GAANNNNTTC 2 cut(s) 462, 585
AspLEI GCGC 3 cut(s) 114, 434, 783
AsuC2I CCSGG 1 cut(s) 342
AsuII TTCGAA 2 cut(s) 156, 700
AvaI CYCGRG 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 107
BalI TGGCCA 1 cut(s) 1010
BanII GRGCYC 1 cut(s) 324
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 380, 897
Bbv12I GWGCWC 2 cut(s) 324, 549
BbvI GCAGC 2 cut(s) 590, 593
BccI CCATC 4 cut(s) 18, 464, 653, 789
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 175
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 1 cut(s) 342
BfaI CTAG 3 cut(s) 119, 941, 1107
BfuAI ACCTGC 1 cut(s) 867
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 3 cut(s) 147, 604, 607
BlsI GCNGC 3 cut(s) 148, 605, 608
Bme1390I CCNGG 2 cut(s) 175, 342
BmeT110I CYCGRG 1 cut(s) 323
BmrFI CCNGG 2 cut(s) 175, 342
BmsI GCATC 5 cut(s) 419, 643, 957, 1034, 1069
BpiI GAAGAC 2 cut(s) 380, 897
Bpu14I TTCGAA 2 cut(s) 156, 700
BpuMI CCSGG 1 cut(s) 342
Bsa29I ATCGAT 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 340, 435, 572
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bse1I ACTGG 3 cut(s) 288, 392, 1140
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 1 cut(s) 175
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 340, 435, 572
BseGI GGATG 7 cut(s) 10, 41, 292, 658, 817, 948, 1071
BseJI GATNNNNATC 1 cut(s) 474
BseNI ACTGG 3 cut(s) 288, 392, 1140
BseRI GAGGAG 1 cut(s) 948
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 2 cut(s) 590, 593
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 48, 92, 1010
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 2 cut(s) 324, 549
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 2 cut(s) 45, 342
BslFI GGGAC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 3 cut(s) 48, 92, 1010
BsoBI CYCGRG 1 cut(s) 323
Bsp119I TTCGAA 2 cut(s) 156, 700
Bsp1286I GDGCHC 3 cut(s) 107, 324, 549
Bsp143I GATC 4 cut(s) 347, 706, 1093, 1123
Bsp19I CCATGG 1 cut(s) 572
BspACI CCGC 4 cut(s) 77, 147, 422, 862
BspANI GGCC 3 cut(s) 48, 92, 1010
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 714
BspMI ACCTGC 1 cut(s) 867
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 978
BspT104I TTCGAA 2 cut(s) 156, 700
BsrI ACTGG 3 cut(s) 288, 392, 1140
BssECI CCNNGG 3 cut(s) 340, 435, 572
BssMI GATC 4 cut(s) 347, 706, 1093, 1123
BssNI GRCGYC 1 cut(s) 225
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 572
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 2 cut(s) 386, 538
Bst6I CTCTTC 1 cut(s) 978
BstACI GRCGYC 1 cut(s) 225
BstBI TTCGAA 2 cut(s) 156, 700
BstC8I GCNNGC 1 cut(s) 1032
BstDEI CTNAG 5 cut(s) 108, 425, 486, 830, 1140
BstDSI CCRYGG 1 cut(s) 572
BstF5I GGATG 7 cut(s) 10, 41, 292, 658, 817, 948, 1071
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 3 cut(s) 114, 434, 783
BstKTI GATC 4 cut(s) 350, 709, 1096, 1126
BstMBI GATC 4 cut(s) 347, 706, 1093, 1123
BstMWI GCNNNNNNNGC 5 cut(s) 111, 626, 737, 945, 1031
BstNI CCWGG 1 cut(s) 175
BstSCI CCNGG 2 cut(s) 173, 340
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 590, 593
BstV2I GAAGAC 2 cut(s) 380, 897
Bsu15I ATCGAT 1 cut(s) 183
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 3 cut(s) 48, 92, 1010
BsuTUI ATCGAT 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 572
BtgZI GCGATG 1 cut(s) 1011
BtsCI GGATG 7 cut(s) 10, 41, 292, 658, 817, 948, 1071
BtsIMutI CAGTG 1 cut(s) 399
BveI ACCTGC 1 cut(s) 867
Cac8I GCNNGC 1 cut(s) 1032
CaiI CAGNNNCTG 1 cut(s) 603
CciI TCATGA 1 cut(s) 714
CfoI GCGC 3 cut(s) 114, 434, 783
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 3 cut(s) 103, 214, 609
Csp6I GTAC 3 cut(s) 194, 747, 1018
CviAII CATG 6 cut(s) 191, 525, 573, 715, 808, 1012
CviQI GTAC 3 cut(s) 194, 747, 1018
DdeI CTNAG 5 cut(s) 108, 425, 486, 830, 1140
DpnI GATC 4 cut(s) 349, 708, 1095, 1125
DpnII GATC 4 cut(s) 347, 706, 1093, 1123
DraI TTTAAA 1 cut(s) 372
EaeI YGGCCR 2 cut(s) 46, 1008
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 572
Eco24I GRGCYC 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 6 cut(s) 194, 528, 576, 718, 811, 1015
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 49
FatI CATG 6 cut(s) 190, 524, 572, 714, 807, 1011
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 147, 604, 607
FokI GGATG 6 cut(s) 28, 279, 665, 824, 935, 1078
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 3 cut(s) 147, 604, 607
FspBI CTAG 3 cut(s) 119, 941, 1107
GlaI GCGC 3 cut(s) 113, 433, 782
GluI GCNGC 3 cut(s) 147, 604, 607
HaeIII GGCC 3 cut(s) 48, 92, 1010
HapII CCGG 2 cut(s) 45, 342
HgaI GACGC 3 cut(s) 103, 214, 609
HhaI GCGC 3 cut(s) 114, 434, 783
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 6 cut(s) 194, 528, 576, 718, 811, 1015
Hin6I GCGC 3 cut(s) 112, 432, 781
HinP1I GCGC 3 cut(s) 112, 432, 781
HincII GTYRAC 2 cut(s) 281, 801
HindII GTYRAC 2 cut(s) 281, 801
HinfI GANTC 4 cut(s) 70, 185, 494, 1102
HpaII CCGG 2 cut(s) 45, 342
Hpy166II GTNNAC 4 cut(s) 241, 281, 801, 1090
Hpy188I TCNGA 4 cut(s) 352, 439, 599, 1045
Hpy188III TCNNGA 6 cut(s) 86, 446, 467, 633, 665, 715
Hpy8I GTNNAC 4 cut(s) 241, 281, 801, 1090
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 8 cut(s) 135, 163, 215, 306, 619, 662, 818, 880
HpyCH4III ACNGT 2 cut(s) 386, 538
HpyCH4IV ACGT 2 cut(s) 237, 642
HpyCH4V TGCA 5 cut(s) 656, 788, 994, 1025, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 626, 737, 945, 1031
HpyF3I CTNAG 5 cut(s) 108, 425, 486, 830, 1140
HpySE526I ACGT 2 cut(s) 237, 642
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 6 cut(s) 194, 528, 576, 718, 811, 1015
HspAI GCGC 3 cut(s) 112, 432, 781
Kzo9I GATC 4 cut(s) 347, 706, 1093, 1123
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 2 cut(s) 424, 916
Lsp1109I GCAGC 2 cut(s) 590, 593
LweI GCATC 5 cut(s) 419, 643, 957, 1034, 1069
MaeI CTAG 3 cut(s) 119, 941, 1107
MaeII ACGT 2 cut(s) 237, 642
MaeIII GTNAC 3 cut(s) 380, 899, 1109
MalI GATC 4 cut(s) 349, 708, 1095, 1125
MboI GATC 4 cut(s) 347, 706, 1093, 1123
MboII GAAGA 6 cut(s) 380, 470, 513, 578, 902, 965
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 3 cut(s) 107, 324, 549
MlsI TGGCCA 1 cut(s) 1010
MluCI AATT 9 cut(s) 81, 287, 374, 440, 462, 591, 615, 645, 927
MluNI TGGCCA 1 cut(s) 1010
Mox20I TGGCCA 1 cut(s) 1010
MroXI GAANNNNTTC 2 cut(s) 462, 585
MscI TGGCCA 1 cut(s) 1010
MseI TTAA 5 cut(s) 234, 371, 401, 681, 1001
MslI CAYNNNNRTG 1 cut(s) 812
Msp20I TGGCCA 1 cut(s) 1010
MspI CCGG 2 cut(s) 45, 342
MspR9I CCNGG 2 cut(s) 175, 342
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 111, 626, 737, 945, 1031
NciI CCSGG 1 cut(s) 342
NcoI CCATGG 1 cut(s) 572
NdeII GATC 4 cut(s) 347, 706, 1093, 1123
NlaIII CATG 6 cut(s) 194, 528, 576, 718, 811, 1015
NspV TTCGAA 2 cut(s) 156, 700
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 978
PcsI WCGNNNNNNNCGW 2 cut(s) 234, 900
PdmI GAANNNNTTC 2 cut(s) 462, 585
PfeI GAWTC 4 cut(s) 70, 185, 494, 1102
PkrI GCNGC 3 cut(s) 148, 605, 608
Psp124BI GAGCTC 1 cut(s) 324
Psp1406I AACGTT 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PspXI VCTCGAGB 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 603
RsaI GTAC 3 cut(s) 195, 748, 1019
RsaNI GTAC 3 cut(s) 194, 747, 1018
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 324
SalI GTCGAC 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 5 cut(s) 234, 371, 401, 681, 1001
SatI GCNGC 3 cut(s) 147, 604, 607
Sau3AI GATC 4 cut(s) 347, 706, 1093, 1123
ScrFI CCNGG 2 cut(s) 175, 342
SduI GDGCHC 3 cut(s) 107, 324, 549
SfaNI GCATC 5 cut(s) 419, 643, 957, 1034, 1069
Sfr274I CTCGAG 1 cut(s) 323
SfuI TTCGAA 2 cut(s) 156, 700
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 9 cut(s) 81, 287, 374, 440, 462, 591, 615, 645, 927
SsiI CCGC 4 cut(s) 77, 147, 422, 862
SspMI CTAG 3 cut(s) 119, 941, 1107
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 173, 340
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 2 cut(s) 386, 538
TaiI ACGT 2 cut(s) 240, 645
TaqI TCGA 5 cut(s) 156, 183, 280, 324, 700
TasI AATT 9 cut(s) 81, 287, 374, 440, 462, 591, 615, 645, 927
TatI WGTACW 1 cut(s) 1017
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 70, 185, 494, 1102
Tru1I TTAA 5 cut(s) 234, 371, 401, 681, 1001
Tru9I TTAA 5 cut(s) 234, 371, 401, 681, 1001
TscAI CASTG 1 cut(s) 399
TseI GCWGC 2 cut(s) 603, 606
TspDTI ATGAA 5 cut(s) 71, 281, 513, 703, 855
TspRI CASTG 1 cut(s) 399
XapI RAATTY 5 cut(s) 81, 287, 374, 440, 462
XhoI CTCGAG 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 2 cut(s) 462, 585
XspI CTAG 3 cut(s) 119, 941, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.