Rorug02G0187800

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010422
Physical Location & Seq
Forward (+)
17502363 .. 17506784
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0187800.1

Sequence Viewer

Length: 1575 bp
ATGGCTTCTCTCTCCTGCGACCAGTCTCTCTATCGCCGCCGTCCAATTCAACCGTTTCCAAGGCGTTATCTCTTACTATTCTATTACCGGACCGCCGGAACTCGGTGGTTCGGCACCAGCACCGCCGAGTCAACTCGGACCGAGTTCACTGAAGAGAACGCCTACGAGCTCTTAGGCGTTTCGGAGACGAGCTCATTCGCCGAAATCAACGCGTCGTTTCATAAATTGGCGAAACAGACTCACCCGGACCTCGTTCAGTCCGGTAACGATTCCGACGTTTCTCGCCGATTCGTTCAAATCCTCGCCGCCTATGAGATTCTTTCGGATTGCAAAAGGAGGGCGCATTATGACAGGGACCTGTTATCTCAGCGAAAGCTTATGCAGAAGCAATCCGGGGGAGGTGGTGCATTGCACTTGTATAAGTTTCATAGTACATATAGGGGGATGGAAGTTGTTGAGTGGCTGAAATGGTATAGAATTGCTATAAGTGATGTGTTGGCGGAGAGGAAGGTGGTTGTTGGAACAGGTTATTTTGATGGGCTTGAGAGAGATTTTTATTCAGCTATACATGCGGCGTATTATGGTCCTGATATTGAGTCTATCGACTTTCTTCCCGACTGCTTTGAAGCTGAAGAGAGGTCTGTGTATGAAACTCCGGAGGTGCTGCATTTGGTTTGGGGGCGTGATATTTTTGGGATGGTCTGCCTAGTAGATAAAGTTCCAGAGCTGCCATATTATAGCAATAAGAGGTTAAAGTATTCCACATCAGCGGGTTTAGGTATTTGCCAATCTGGTGAGGATTTGAGAATCTCTATGAGCTCGGGTGGTGTTTATAATGATGGATATGCCCATATGCAAGCTGCGGATGTCTCAACCCACACATCTGATCCTTATAGAGATTTAGAATTGCATATATCTGGAAGGGTGGTTGCTACAGCCACAAGAGTCCCTCCCAAAAGCCATCGTGACCGGATACAGAATGAAGACACCGATCACATTCACGTTTTTCTGAGCTCTGATGATAATCCTGTGCATACCAGCAAAGGGTTTACTGAAGCTTCCTCTTCAGGCAGTGCTGTGGGATCAAGGATTCTATTGGGAACTATAACTGGATTGCGGTCTAGCTCAGAAGAAGGGTCTTGCCTTGTCTACAATAGCAGTGGTACCAAAACCCATGTGATTATGAAGCATAGAACACTTCTGGTGAAACACATGCATTGGTATGGATTGGGAGACAATGTTTCTGTATGTGAGTGTAGATGCACAAGAGCACAATTACCACCAAGCAAATTTTGGTTGTTTGAGCCTCGTTGTGGCATGCATGACATAGGCGGTTGGTATGTTGAAACATTTGGCAAAGATAAGAAAGGACGGTTATCACCATCACAGAGATATTGGGATGGCTTTGATGCAAGGGAACAATCTGAGAAGAGACTCCATCCTGCGATGTACCTTCTTGCTATGGGATATAGAACTTTAGATCTTGAAGATGCAAAAAGAAGGAAACATACATTCAGGGATGTTGTCGACAGAAAGCTCTTTAAAATACTCAATTGGTGCAAAAGACTTGTTTAA

Protein Analysis

524

Amino Acids

59.85

Weight (kDa)

8.34

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 53 - 117 7.2e-17 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
Acc65I GGTACC 1 cut(s) 1163
AccB1I GGYRCC 2 cut(s) 113, 1163
AccI GTMKAC 2 cut(s) 1149, 1527
AccII CGCG 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 655
AclWI GGATC 2 cut(s) 881, 1090
AcsI RAATTY 1 cut(s) 1289
AcuI CTGAAG 4 cut(s) 171, 651, 1050, 1074
AfaI GTAC 3 cut(s) 433, 1165, 1451
AfiI CCNNNNNNNGG 3 cut(s) 102, 1044, 1313
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 5 cut(s) 50, 296, 626, 1346, 1487
Alw21I GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
Alw26I GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
AlwI GGATC 2 cut(s) 881, 1090
Ama87I CYCGRG 1 cut(s) 820
Aor13HI TCCGGA 1 cut(s) 655
ApeKI GCWGC 3 cut(s) 664, 727, 860
ApoI RAATTY 1 cut(s) 1289
Asp718I GGTACC 1 cut(s) 1163
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 5 cut(s) 90, 138, 247, 355, 584
AsuC2I CCSGG 2 cut(s) 245, 394
AsuHPI GGTGA 4 cut(s) 233, 806, 1216, 1371
AvaI CYCGRG 1 cut(s) 820
AvaII GGWCC 5 cut(s) 90, 138, 247, 355, 584
BanI GGYRCC 2 cut(s) 113, 1163
BanII GRGCYC 4 cut(s) 171, 194, 821, 1016
BarI GAAGNNNNNNTAC 2 cut(s) 1492, 1524
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
BbvI GCAGC 3 cut(s) 651, 714, 847
BccI CCATC 8 cut(s) 439, 530, 691, 833, 969, 1390, 1394, 1446
BceAI ACGGC 1 cut(s) 24
BciVI GTATCC 1 cut(s) 966
BcnI CCSGG 2 cut(s) 245, 394
BcoDI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BfaI CTAG 2 cut(s) 707, 1122
BfmI CTRYAG 1 cut(s) 933
BfuI GTATCC 1 cut(s) 966
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
BlsI GCNGC 6 cut(s) 38, 307, 574, 666, 729, 862
Bme1390I CCNGG 2 cut(s) 245, 394
Bme18I GGWCC 5 cut(s) 90, 138, 247, 355, 584
BmeT110I CYCGRG 1 cut(s) 820
BmgT120I GGNCC 5 cut(s) 90, 138, 247, 355, 584
BmiI GGNNCC 3 cut(s) 115, 356, 1165
BmrFI CCNGG 2 cut(s) 245, 394
BmsI GCATC 3 cut(s) 1250, 1399, 1480
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 245, 394
BsaBI GATNNNNATC 1 cut(s) 1023
BsaJI CCNNGG 2 cut(s) 59, 393
BsaWI WCCGGW 4 cut(s) 87, 260, 655, 969
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 1044, 1313
Bse1I ACTGG 2 cut(s) 22, 1114
Bse3DI GCAATG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 59, 393
BseGI GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 3 cut(s) 102, 1044, 1313
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 4 cut(s) 380, 1001, 1140, 1416
BseNI ACTGG 2 cut(s) 22, 1114
BseXI GCAGC 3 cut(s) 651, 714, 847
Bsh1236I CGCG 1 cut(s) 212
BshNI GGYRCC 2 cut(s) 113, 1163
BsiHKAI GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
BsiHKCI CYCGRG 1 cut(s) 820
BsiSI CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
BslFI GGGAC 2 cut(s) 368, 932
BslI CCNNNNNNNGG 3 cut(s) 102, 1044, 1313
BsmAI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 368, 932
BsoBI CYCGRG 1 cut(s) 820
Bsp1286I GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 4 cut(s) 886, 991, 1082, 1480
BspCNI CTCAG 4 cut(s) 379, 1002, 1139, 1417
BspEI TCCGGA 1 cut(s) 655
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 3 cut(s) 115, 356, 1165
BspPI GGATC 2 cut(s) 881, 1090
BspT107I GGYRCC 2 cut(s) 113, 1163
BsrDI GCAATG 1 cut(s) 407
BsrI ACTGG 2 cut(s) 22, 1114
BssECI CCNNGG 2 cut(s) 59, 393
BssMI GATC 4 cut(s) 886, 991, 1082, 1480
BssT1I CCWWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 54, 1374
Bst6I CTCTTC 4 cut(s) 147, 627, 1069, 1424
BstC8I GCNNGC 2 cut(s) 858, 1319
BstDEI CTNAG 5 cut(s) 172, 366, 1010, 1126, 1425
BstF5I GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 889, 994, 1085, 1483
BstMAI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BstMBI GATC 4 cut(s) 886, 991, 1082, 1480
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNSI RCATGY 3 cut(s) 572, 1216, 1321
BstSCI CCNGG 2 cut(s) 243, 392
BstSFI CTRYAG 1 cut(s) 933
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 651, 714, 847
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 1480
BstYI RGATCY 1 cut(s) 1480
BsuI GTATCC 1 cut(s) 966
BtgZI GCGATG 1 cut(s) 1460
BtsCI GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BtsI GCAGTG 2 cut(s) 1078, 1165
BtsIMutI CAGTG 3 cut(s) 147, 1078, 1165
Cac8I GCNNGC 2 cut(s) 858, 1319
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 5 cut(s) 90, 138, 247, 355, 584
CpoI CGGWCCG 2 cut(s) 90, 138
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 432, 1164, 1450
CspCI CAANNNNNGTGG 2 cut(s) 1141, 1176
CspI CGGWCCG 2 cut(s) 90, 138
CviAII CATG 5 cut(s) 569, 1175, 1213, 1318, 1322
CviQI GTAC 3 cut(s) 432, 1164, 1450
DdeI CTNAG 5 cut(s) 172, 366, 1010, 1126, 1425
DpnI GATC 4 cut(s) 888, 993, 1084, 1482
DpnII GATC 4 cut(s) 886, 991, 1082, 1480
DraI TTTAAA 1 cut(s) 1543
Eam1104I CTCTTC 4 cut(s) 147, 627, 1069, 1424
EarI CTCTTC 4 cut(s) 147, 627, 1069, 1424
EciI GGCGGA 1 cut(s) 515
Ecl136II GAGCTC 4 cut(s) 169, 192, 819, 1014
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 4 cut(s) 171, 194, 821, 1016
Eco47I GGWCC 5 cut(s) 90, 138, 247, 355, 584
Eco53kI GAGCTC 4 cut(s) 169, 192, 819, 1014
Eco57I CTGAAG 4 cut(s) 171, 651, 1050, 1074
Eco88I CYCGRG 1 cut(s) 820
EcoICRI GAGCTC 4 cut(s) 169, 192, 819, 1014
EcoO109I RGGNCCY 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 2 cut(s) 1218, 1323
EcoT38I GRGCYC 4 cut(s) 171, 194, 821, 1016
ErhI CCWWGG 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
FaqI GGGAC 2 cut(s) 368, 932
FatI CATG 5 cut(s) 568, 1174, 1212, 1317, 1321
FauI CCCGC 1 cut(s) 763
FauNDI CATATG 1 cut(s) 852
FblI GTMKAC 2 cut(s) 1149, 1527
Fnu4HI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
FokI GGATG 6 cut(s) 457, 709, 878, 1412, 1425, 1532
FriOI GRGCYC 4 cut(s) 171, 194, 821, 1016
Fsp4HI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
FspBI CTAG 2 cut(s) 707, 1122
GlaI GCGC 1 cut(s) 342
GluI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
HapII CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
HgaI GACGC 1 cut(s) 201
HhaI GCGC 1 cut(s) 343
Hin1II CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 2 cut(s) 132, 1528
HindII GTYRAC 2 cut(s) 132, 1528
HindIII AAGCTT 2 cut(s) 374, 1056
HpaII CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
HphI GGTGA 4 cut(s) 233, 806, 1216, 1371
Hpy166II GTNNAC 5 cut(s) 132, 147, 1050, 1150, 1528
Hpy188I TCNGA 9 cut(s) 138, 184, 274, 325, 886, 1011, 1018, 1129, 1426
Hpy188III TCNNGA 7 cut(s) 587, 614, 656, 722, 918, 965, 1484
Hpy8I GTNNAC 5 cut(s) 132, 147, 1050, 1150, 1528
Hpy99I CGWCG 2 cut(s) 217, 278
HpyAV CCTTC 5 cut(s) 502, 915, 1127, 1463, 1494
HpyCH4III ACNGT 2 cut(s) 54, 1374
HpyCH4IV ACGT 2 cut(s) 276, 1002
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 5 cut(s) 172, 366, 1010, 1126, 1425
HpySE526I ACGT 2 cut(s) 276, 1002
Hsp92II CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
HspAI GCGC 1 cut(s) 341
Kpn2I TCCGGA 1 cut(s) 655
KpnI GGTACC 1 cut(s) 1167
Kzo9I GATC 4 cut(s) 886, 991, 1082, 1480
Lsp1109I GCAGC 3 cut(s) 651, 714, 847
LweI GCATC 3 cut(s) 1250, 1399, 1480
MaeI CTAG 2 cut(s) 707, 1122
MaeII ACGT 2 cut(s) 276, 1002
MaeIII GTNAC 2 cut(s) 263, 965
MalI GATC 4 cut(s) 888, 993, 1084, 1482
MboI GATC 4 cut(s) 886, 991, 1082, 1480
MboII GAAGA 8 cut(s) 164, 602, 644, 995, 1056, 1142, 1441, 1499
MfeI CAATTG 1 cut(s) 1552
MflI RGATCY 1 cut(s) 1480
MhlI GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
MluCI AATT 7 cut(s) 45, 224, 477, 905, 1274, 1289, 1552
MluI ACGCGT 1 cut(s) 210
MlyI GAGTC 5 cut(s) 137, 232, 605, 954, 1428
MmeI TCCRAC 2 cut(s) 297, 499
Mph1103I ATGCAT 2 cut(s) 1218, 1323
MroI TCCGGA 1 cut(s) 655
MseI TTAA 3 cut(s) 752, 1542, 1573
MslI CAYNNNNRTG 1 cut(s) 1221
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
MspR9I CCNGG 2 cut(s) 245, 394
MunI CAATTG 1 cut(s) 1552
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 569
NciI CCSGG 2 cut(s) 245, 394
NdeI CATATG 1 cut(s) 852
NdeII GATC 4 cut(s) 886, 991, 1082, 1480
NlaIII CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
NlaIV GGNNCC 3 cut(s) 115, 356, 1165
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 965
NsiI ATGCAT 2 cut(s) 1218, 1323
NspI RCATGY 3 cut(s) 572, 1216, 1321
PaeI GCATGC 1 cut(s) 1321
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 5 cut(s) 269, 288, 316, 807, 1090
PkrI GCNGC 6 cut(s) 38, 307, 574, 666, 729, 862
PleI GAGTC 5 cut(s) 136, 232, 604, 953, 1428
PpsI GAGTC 5 cut(s) 136, 232, 604, 953, 1428
PpuMI RGGWCCY 1 cut(s) 355
PsiI TTATAA 1 cut(s) 834
Psp124BI GAGCTC 4 cut(s) 171, 194, 821, 1016
Psp5II RGGWCCY 1 cut(s) 355
PspN4I GGNNCC 3 cut(s) 115, 356, 1165
PspPI GGNCC 5 cut(s) 90, 138, 247, 355, 584
PspPPI RGGWCCY 1 cut(s) 355
PsrI GAACNNNNNNTAC 2 cut(s) 702, 734
PsuI RGATCY 1 cut(s) 1480
RsaI GTAC 3 cut(s) 433, 1165, 1451
RsaNI GTAC 3 cut(s) 432, 1164, 1450
RseI CAYNNNNRTG 1 cut(s) 1221
Rsr2I CGGWCCG 2 cut(s) 90, 138
RsrII CGGWCCG 2 cut(s) 90, 138
SacI GAGCTC 4 cut(s) 171, 194, 821, 1016
SalI GTCGAC 1 cut(s) 1526
SaqAI TTAA 3 cut(s) 752, 1542, 1573
SatI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
Sau3AI GATC 4 cut(s) 886, 991, 1082, 1480
Sau96I GGNCC 5 cut(s) 90, 138, 247, 355, 584
SchI GAGTC 5 cut(s) 137, 232, 605, 954, 1428
ScrFI CCNGG 2 cut(s) 245, 394
SduI GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
SfaNI GCATC 3 cut(s) 1250, 1399, 1480
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 5 cut(s) 90, 138, 247, 355, 584
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SphI GCATGC 1 cut(s) 1321
Sse9I AATT 7 cut(s) 45, 224, 477, 905, 1274, 1289, 1552
SspMI CTAG 2 cut(s) 707, 1122
SstI GAGCTC 4 cut(s) 171, 194, 821, 1016
StyD4I CCNGG 2 cut(s) 243, 392
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 54, 1374
TaiI ACGT 2 cut(s) 279, 1005
TaqI TCGA 2 cut(s) 603, 1527
TaqII GACCGA 1 cut(s) 155
TasI AATT 7 cut(s) 45, 224, 477, 905, 1274, 1289, 1552
TatI WGTACW 1 cut(s) 431
TauI GCSGC 3 cut(s) 39, 308, 575
TfiI GAWTC 5 cut(s) 269, 288, 316, 807, 1090
Tru1I TTAA 3 cut(s) 752, 1542, 1573
Tru9I TTAA 3 cut(s) 752, 1542, 1573
TscAI CASTG 3 cut(s) 154, 1078, 1165
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 3 cut(s) 664, 727, 860
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 5 cut(s) 209, 416, 663, 996, 1199
TspRI CASTG 3 cut(s) 154, 1078, 1165
VpaK11BI GGWCC 5 cut(s) 90, 138, 247, 355, 584
XapI RAATTY 1 cut(s) 1289
XceI RCATGY 3 cut(s) 572, 1216, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1290
XmiI GTMKAC 2 cut(s) 1149, 1527
XspI CTAG 2 cut(s) 707, 1122
Zsp2I ATGCAT 2 cut(s) 1218, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.