AT5G10870

chorismate mutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010296
Physical Location & Seq
Reverse (-)
3430497 .. 3432468
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10870.1

Sequence Viewer

Length: 798 bp
ATGGCAAGAGTCTTCGAATCGGATTCGGGTTCTGGTTGTTCCAATGTACTGAGTCTTGACTTAATCAGAGAATCGTTGATTAGGCAAGAAGACACCATCGTCTTCAGCTTGATCGAGAGAGCTAAGTTTCCACTCAATTCTCCTGCTTTCGAGGAATCTCGTTGTCTAGATTCTGGAAGTTTCTCTTCTCTCACTGAGTTTTTCGTCAGAGAGACAGAAATCATCCAAGCTAAGGTAGGAAGATATGAATACCCGGAAGAGAATCCTTTCTTCCTTGAGAACATTCCTCACTCGGTTTTTCCTACGCACAAATATCCATCGGCTTTGCACCCTAAGGCTCTATCTGTTAACATTAACAAACAAATCTGGGATATTTACTTTAAAGAATTGCTTCCTTTGTTTGTCAAACCTGGCGATGATGGCAACTATCCATCAACTGCTGCTAGTGATCTCGCCTGTTTACAAGCTCTTTCGAGAAGGATTCACTACGGTAAATTTGTAGCTGAGGTCAAATTCAGAGATGCTCCACAAGATTACGAGCCTGCGATTCGCGCTCAGGATAGAGAGGCTTTGATGAAGCTGTTGACGTTTGAGAAAGTAGAAGAAATGGTTAAGAAGAGAGTGCAGAAGAAAGCAGAAACGTTTGGACAAGAAGTAAAATTCAACTCTGGCTATGGCGATGAGAGTAAGAAGAAGTATAAAGTGGATCCATTGCTTGCCTCTCGCATCTACGGGGAATGGCTTATCCCTCTCACTAAGCTCGTTGAGGTTGAGTATCTTCTACGTCGTCTCGATTGA

Protein Analysis

265

Amino Acids

30.49

Weight (kDa)

5.95

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CM_2 PF01817 146 - 256 1.1e-08 Chorismate mutase type II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccII CGCG 1 cut(s) 552
AclI AACGTT 1 cut(s) 641
AclWI GGATC 2 cut(s) 701, 714
AcsI RAATTY 3 cut(s) 494, 512, 659
AcuI CTGAAG 1 cut(s) 88
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 664
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 7 cut(s) 108, 122, 230, 467, 503, 580, 760
AluI AGCT 7 cut(s) 108, 122, 230, 467, 503, 580, 760
Alw26I GTCTC 2 cut(s) 206, 794
AlwI GGATC 2 cut(s) 701, 714
ApeKI GCWGC 1 cut(s) 440
ApoI RAATTY 3 cut(s) 494, 512, 659
Asp700I GAANNNNTTC 2 cut(s) 266, 390
AspLEI GCGC 1 cut(s) 554
AsuC2I CCSGG 1 cut(s) 254
AsuII TTCGAA 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 333
BamHI GGATCC 1 cut(s) 706
BbsI GAAGAC 3 cut(s) 4, 94, 96
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 427
BccI CCATC 4 cut(s) 104, 325, 413, 439
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 206, 794
BfaI CTAG 2 cut(s) 167, 444
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme1390I CCNGG 2 cut(s) 254, 411
BmiI GGNNCC 1 cut(s) 708
BmrFI CCNGG 2 cut(s) 254, 411
BmsI GCATC 2 cut(s) 511, 735
BpiI GAAGAC 3 cut(s) 4, 94, 96
Bpu10I CCTNAGC 3 cut(s) 231, 504, 555
Bpu14I TTCGAA 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 254
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 710
BseBI CCWGG 1 cut(s) 411
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 1 cut(s) 710
BseMII CTCAG 4 cut(s) 41, 186, 495, 569
BseXI GCAGC 1 cut(s) 427
BsgI GTGCAG 1 cut(s) 644
Bsh1236I CGCG 1 cut(s) 552
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 2 cut(s) 206, 794
BsmBI CGTCTC 1 cut(s) 794
Bsp119I TTCGAA 1 cut(s) 15
Bsp143I GATC 3 cut(s) 111, 448, 706
BspCNI CTCAG 4 cut(s) 42, 187, 496, 568
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 1 cut(s) 708
BspPI GGATC 2 cut(s) 701, 714
BspT104I TTCGAA 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 710
BssMI GATC 3 cut(s) 111, 448, 706
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 3 cut(s) 190, 252, 611
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 543, 717
BstDEI CTNAG 8 cut(s) 50, 123, 195, 231, 333, 504, 555, 756
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 554
BstKTI GATC 3 cut(s) 114, 451, 709
BstMAI GTCTC 2 cut(s) 206, 794
BstMBI GATC 3 cut(s) 111, 448, 706
BstMWI GCNNNNNNNGC 2 cut(s) 420, 551
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 252, 409
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 427
BstV2I GAAGAC 3 cut(s) 4, 94, 96
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
Bsu36I CCTNAGG 1 cut(s) 333
BtgZI GCGATG 2 cut(s) 429, 693
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 543, 717
CfoI GCGC 1 cut(s) 554
Csp6I GTAC 1 cut(s) 47
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 8 cut(s) 50, 123, 195, 231, 333, 504, 555, 756
DpnI GATC 3 cut(s) 113, 450, 708
DpnII GATC 3 cut(s) 111, 448, 706
DraI TTTAAA 1 cut(s) 382
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 3 cut(s) 190, 252, 611
EarI CTCTTC 3 cut(s) 190, 252, 611
Eco57I CTGAAG 1 cut(s) 88
Eco81I CCTNAGG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 794
FaiI YATR 3 cut(s) 246, 675, 699
FalI AAGNNNNNCTT 4 cut(s) 169, 201, 375, 407
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 441
FspBI CTAG 2 cut(s) 167, 444
GlaI GCGC 1 cut(s) 553
GluI GCNGC 1 cut(s) 441
HapII CCGG 1 cut(s) 254
HhaI GCGC 1 cut(s) 554
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HincII GTYRAC 2 cut(s) 349, 585
HindII GTYRAC 2 cut(s) 349, 585
HpaI GTTAAC 1 cut(s) 349
HpaII CCGG 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 349, 461, 585
Hpy188I TCNGA 4 cut(s) 22, 68, 209, 518
Hpy188III TCNNGA 7 cut(s) 56, 115, 167, 174, 474, 557, 791
Hpy8I GTNNAC 3 cut(s) 349, 461, 585
Hpy99I CGWCG 1 cut(s) 789
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 3 cut(s) 587, 641, 784
HpyCH4V TGCA 2 cut(s) 328, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 420, 551
HpyF3I CTNAG 8 cut(s) 50, 123, 195, 231, 333, 504, 555, 756
HpySE526I ACGT 3 cut(s) 587, 641, 784
HspAI GCGC 1 cut(s) 552
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 3 cut(s) 111, 448, 706
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 2 cut(s) 511, 735
MaeI CTAG 2 cut(s) 167, 444
MaeII ACGT 3 cut(s) 587, 641, 784
MalI GATC 3 cut(s) 113, 450, 708
MboI GATC 3 cut(s) 111, 448, 706
MflI RGATCY 1 cut(s) 706
MluCI AATT 5 cut(s) 136, 386, 494, 512, 659
MlyI GAGTC 2 cut(s) 18, 61
MnlI CCTC 7 cut(s) 145, 297, 499, 559, 730, 759, 760
MroXI GAANNNNTTC 2 cut(s) 266, 390
MseI TTAA 5 cut(s) 62, 348, 354, 381, 612
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 254, 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 420, 551
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 111, 448, 706
NlaIV GGNNCC 1 cut(s) 708
NspV TTCGAA 1 cut(s) 15
PdmI GAANNNNTTC 2 cut(s) 266, 390
PfeI GAWTC 8 cut(s) 17, 23, 71, 155, 170, 262, 481, 547
PkrI GCNGC 1 cut(s) 442
PleI GAGTC 2 cut(s) 17, 60
PpsI GAGTC 2 cut(s) 17, 60
Psp1406I AACGTT 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 708
PsuI RGATCY 1 cut(s) 706
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SaqAI TTAA 5 cut(s) 62, 348, 354, 381, 612
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 3 cut(s) 111, 448, 706
SchI GAGTC 2 cut(s) 18, 61
ScrFI CCNGG 2 cut(s) 254, 411
SfaNI GCATC 2 cut(s) 511, 735
SfuI TTCGAA 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 5 cut(s) 136, 386, 494, 512, 659
SspMI CTAG 2 cut(s) 167, 444
StyD4I CCNGG 2 cut(s) 252, 409
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 3 cut(s) 590, 644, 787
TaqI TCGA 5 cut(s) 15, 114, 150, 473, 792
TasI AATT 5 cut(s) 136, 386, 494, 512, 659
TatI WGTACW 1 cut(s) 46
TfiI GAWTC 8 cut(s) 17, 23, 71, 155, 170, 262, 481, 547
Tru1I TTAA 5 cut(s) 62, 348, 354, 381, 612
Tru9I TTAA 5 cut(s) 62, 348, 354, 381, 612
TscAI CASTG 1 cut(s) 199
TseI GCWGC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 261, 590
TspRI CASTG 1 cut(s) 199
XapI RAATTY 3 cut(s) 494, 512, 659
XbaI TCTAGA 1 cut(s) 166
XmnI GAANNNNTTC 2 cut(s) 266, 390
XspI CTAG 2 cut(s) 167, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.