AT5G15530

first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008330
Physical Location & Seq
Forward (+)
5038563 .. 5040840
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15530.1

Sequence Viewer

Length: 768 bp
ATGGCGTCATTGTCGGTACCTTGCGTCAAGATCTGTGCTCTGAACAGGCGGGTCGGATCTCTCCCTGGAATCTCTACTCAAAGATGGCAGCCGCAACCCAATGGGATCTCCTTTCCCTCCGATGTTTCTCAGAATCATTCTGCATTCTGGAGGTTGCGTGCAACAACTAATGAGGTTGTTTCTAACTCCACTCCAATGACTAATGGTGGGTATATGAACGGAAAAGCGAAAACCAATGTTCCTGAACCCGCCGAGCTCTCTGAATTTATGGCTAAAGTCTCTGGTCTTCTTAAGCTTGTGGATTCAAAAGACATAGTGGAACTTGAACTAAAGCAGCTCGACTGTGAGATCGTTATTCGAAAGAAGGAAGCTTTACAGCAAGCTGTACCACCAGCTCCAGTTTATCACTCAATGCCTCCTGTAATGGCAGACTTTTCAATGCCTCCAGCTCAACCAGTGGCTCTTCCTCCTTCTCCTACTCCTACCTCAACGCCTGCAACAGCAAAACCAACATCCGCCCCATCCTCGTCTCATCCTCCACTCAAGAGTCCCATGGCTGGTACTTTCTATAGATCTCCTGGACCCGGTGAACCCCCTTTTGTAAAGGTTGGAGATAAGGTACAGAAGGGTCAAATTGTTTGTATTATCGAAGCTATGAAACTGATGAACGAGATTGAGGCTGAGAAGTCAGGAACCATCATGGAACTACTGGCTGAAGATGGAAAACCAGTCAGCGTTGACACGCCTCTTTTTGTCATCGCACCTTGA

Protein Analysis

255

Amino Acids

27.28

Weight (kDa)

7.65

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 181 - 253 6.7e-25 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 16
AciI CCGC 4 cut(s) 49, 92, 249, 516
AclWI GGATC 2 cut(s) 64, 113
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 18, 387, 562, 621
AfiI CCNNNNNNNGG 2 cut(s) 557, 584
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 3 cut(s) 306, 326, 438
AjnI CCWGG 2 cut(s) 64, 577
AluBI AGCT 8 cut(s) 256, 295, 337, 371, 383, 395, 449, 653
AluI AGCT 8 cut(s) 256, 295, 337, 371, 383, 395, 449, 653
Alw21I GWGCWC 2 cut(s) 40, 258
Alw26I GTCTC 2 cut(s) 283, 534
AlwI GGATC 2 cut(s) 64, 113
ApeKI GCWGC 2 cut(s) 88, 334
ApoI RAATTY 1 cut(s) 263
Asp718I GGTACC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 1 cut(s) 599
AsuII TTCGAA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 581
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 2 cut(s) 40, 258
BbvI GCAGC 2 cut(s) 100, 346
BccI CCATC 4 cut(s) 78, 529, 704, 713
BciT130I CCWGG 2 cut(s) 66, 579
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 2 cut(s) 283, 534
BfmI CTRYAG 1 cut(s) 568
BfrI CTTAAG 1 cut(s) 290
BglII AGATCT 2 cut(s) 30, 572
BisI GCNGC 3 cut(s) 89, 92, 335
BlsI GCNGC 3 cut(s) 90, 93, 336
Bme1390I CCNGG 3 cut(s) 66, 579, 585
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 18, 583, 694
BmrFI CCNGG 3 cut(s) 66, 579, 585
BpiI GAAGAC 1 cut(s) 278
BpmI CTGGAG 3 cut(s) 169, 381, 429
Bpu14I TTCGAA 1 cut(s) 358
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 64, 552
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 557, 584
Bse1I ACTGG 4 cut(s) 398, 455, 714, 728
BseBI CCWGG 2 cut(s) 66, 579
BseDI CCNNGG 2 cut(s) 64, 552
BseGI GGATG 3 cut(s) 512, 521, 532
BseLI CCNNNNNNNGG 2 cut(s) 557, 584
BseMII CTCAG 2 cut(s) 143, 672
BseNI ACTGG 4 cut(s) 398, 455, 714, 728
BseXI GCAGC 2 cut(s) 100, 346
BshNI GGYRCC 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 40, 258
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 2 cut(s) 557, 584
BsmAI GTCTC 2 cut(s) 283, 534
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 534
BsmI GAATGC 1 cut(s) 143
Bsp119I TTCGAA 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 40, 258
Bsp143I GATC 5 cut(s) 30, 56, 105, 348, 572
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 4 cut(s) 49, 92, 249, 516
BspCNI CTCAG 2 cut(s) 142, 673
BspLI GGNNCC 3 cut(s) 18, 583, 694
BspPI GGATC 2 cut(s) 64, 113
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 16
BspTI CTTAAG 1 cut(s) 290
BsrI ACTGG 4 cut(s) 398, 455, 714, 728
BssECI CCNNGG 2 cut(s) 64, 552
BssMI GATC 5 cut(s) 30, 56, 105, 348, 572
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 66, 579
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 159, 381, 495
BstDEI CTNAG 2 cut(s) 129, 681
BstDSI CCRYGG 1 cut(s) 552
BstF5I GGATG 3 cut(s) 512, 521, 532
BstKTI GATC 5 cut(s) 33, 59, 108, 351, 575
BstMAI GTCTC 2 cut(s) 283, 534
BstMBI GATC 5 cut(s) 30, 56, 105, 348, 572
BstNI CCWGG 2 cut(s) 66, 579
BstSCI CCNGG 3 cut(s) 64, 577, 583
BstSFI CTRYAG 1 cut(s) 568
BstV1I GCAGC 2 cut(s) 100, 346
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 4 cut(s) 30, 56, 105, 572
BstYI RGATCY 4 cut(s) 30, 56, 105, 572
BtgI CCRYGG 1 cut(s) 552
BtgZI GCGATG 1 cut(s) 742
BtsCI GGATG 3 cut(s) 512, 521, 532
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 3 cut(s) 159, 381, 495
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 1 cut(s) 13
Csp6I GTAC 4 cut(s) 17, 386, 561, 620
CviAII CATG 2 cut(s) 553, 700
CviQI GTAC 4 cut(s) 17, 386, 561, 620
DdeI CTNAG 2 cut(s) 129, 681
DpnI GATC 5 cut(s) 32, 58, 107, 350, 574
DpnII GATC 5 cut(s) 30, 56, 105, 348, 572
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EciI GGCGGA 1 cut(s) 505
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 581
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 735
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 64, 577
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 2 cut(s) 556, 703
FaiI YATR 8 cut(s) 213, 215, 269, 314, 554, 570, 656, 701
FaqI GGGAC 1 cut(s) 534
FatI CATG 2 cut(s) 552, 699
FauI CCCGC 2 cut(s) 42, 256
Fnu4HI GCNGC 3 cut(s) 89, 92, 335
FokI GGATG 3 cut(s) 499, 508, 519
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 3 cut(s) 89, 92, 335
GluI GCNGC 3 cut(s) 89, 92, 335
GsuI CTGGAG 3 cut(s) 169, 381, 429
HapII CCGG 1 cut(s) 585
HgaI GACGC 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 556, 703
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 293, 369
HinfI GANTC 4 cut(s) 69, 133, 302, 547
HpaII CCGG 1 cut(s) 585
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 2 cut(s) 590, 739
Hpy188I TCNGA 5 cut(s) 42, 56, 121, 132, 262
Hpy188III TCNNGA 5 cut(s) 28, 148, 242, 544, 690
Hpy8I GTNNAC 2 cut(s) 590, 739
HpyAV CCTTC 3 cut(s) 358, 480, 619
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 3 cut(s) 143, 161, 497
HpyF3I CTNAG 2 cut(s) 129, 681
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 556, 703
KpnI GGTACC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 30, 56, 105, 348, 572
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 2 cut(s) 100, 346
MalI GATC 5 cut(s) 32, 58, 107, 350, 574
MboI GATC 5 cut(s) 30, 56, 105, 348, 572
MboII GAAGA 3 cut(s) 278, 455, 728
MflI RGATCY 4 cut(s) 30, 56, 105, 572
MhlI GDGCHC 2 cut(s) 40, 258
MluCI AATT 2 cut(s) 263, 633
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 2 cut(s) 34, 589
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 3 cut(s) 66, 579, 585
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 2 cut(s) 66, 579
NciI CCSGG 1 cut(s) 585
NcoI CCATGG 1 cut(s) 552
NdeII GATC 5 cut(s) 30, 56, 105, 348, 572
NlaIII CATG 2 cut(s) 556, 703
NlaIV GGNNCC 3 cut(s) 18, 583, 694
NmeAIII GCCGAG 1 cut(s) 277
NspV TTCGAA 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 3 cut(s) 69, 133, 302
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 3 cut(s) 90, 93, 336
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 64, 577
PspGI CCWGG 2 cut(s) 64, 577
PspN4I GGNNCC 3 cut(s) 18, 583, 694
PspPI GGNCC 1 cut(s) 581
PsuI RGATCY 4 cut(s) 30, 56, 105, 572
RsaI GTAC 4 cut(s) 18, 387, 562, 621
RsaNI GTAC 4 cut(s) 17, 386, 561, 620
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 1 cut(s) 258
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 89, 92, 335
Sau3AI GATC 5 cut(s) 30, 56, 105, 348, 572
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 3 cut(s) 66, 579, 585
SduI GDGCHC 2 cut(s) 40, 258
SfcI CTRYAG 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 358
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 290, 542
SmoI CTYRAG 2 cut(s) 290, 542
Sse9I AATT 2 cut(s) 263, 633
SsiI CCGC 4 cut(s) 49, 92, 249, 516
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 3 cut(s) 64, 577, 583
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 3 cut(s) 339, 358, 648
TasI AATT 2 cut(s) 263, 633
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 3 cut(s) 69, 133, 302
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 462
TseI GCWGC 2 cut(s) 88, 334
TspDTI ATGAA 3 cut(s) 230, 671, 680
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 462
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.