MD09G1064200.v1.1

first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008330
Physical Location & Seq
Forward (+)
4398867 .. 4401242
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1064200.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCTTCCTTCACTGTTCCCTGTCCCAAGGCTTGCTCCGCCCCCGTTACCCGCCTCGGCTCCTCCGCCCAGCCGTCGCAGCCGCAGCAGCACCTCCTCTCCTTCCACAACTGCCTCAATCGCTGCCCCATTCCCTCTCTGCAGATTTCTGGCCTTCAGAGCACGGTCAGGAAGCAATCTTCTTCCTGGAAGGTGCAGGCACAGCTTAATGAGGTTGCAGCTGAGAAATCCCCAAATTCTGTACCCATAAACAACAAGAAGTCTGAAGATGGATCACCGGAAGGGAAAGGCGAGTCTTCCAGTCAAACCAATATTCCAGATGCAGCATCAATTTCCGCATTCATGGCTCAAGTATCAGACCTTATTAAGTTAGTGGATTCGAGAGACATTGTGGAGCTGGAAATGAAGCAACTAGATTTAGAGCTTGTGATTAGAAAAAAAGAAGCATTGGCGCCACCACCACCAGCTCCAGCTTCCCAACAACCAGCACAATATTATTCCATGTCACCACCTCAACCAGCAGCAGCAGCAGCTCCTCCTCCAGCACCCGGTCCACCAGCACCTGCACTACCCGCCCCTGCAAAGACAAGCACATCATCTCACCCTCCACTGAAATGCCCCATGGCTGGAACCTTTTACCGCTGTCCTGCACCTGGTGAACCATCTTTTGTAAAGGTTGGAGATAAAGTTCAGAAAGGTCAAGTCATTTGCATCATTGAGGCCATGAAATTGATGAACGAAATTGAGGCTGATCAATCTGGAACCGTTGCTGAGATACTGGTAGAAGATGGGAAACCAGTCAGTGTAGACACGCCTCTTTTTGTCATAGTACCTTGA

Protein Analysis

279

Amino Acids

29.3

Weight (kDa)

6.32

Isoelectric Point (pI)

72.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 204 - 276 2.3e-25 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 571
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 807
AciI CCGC 7 cut(s) 39, 52, 66, 83, 336, 573, 640
AclWI GGATC 1 cut(s) 280
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 140, 285
AcyI GRCGYC 1 cut(s) 452
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 2 cut(s) 243, 831
AfiI CCNNNNNNNGG 3 cut(s) 548, 626, 653
AjnI CCWGG 2 cut(s) 185, 652
AloI GAACNNNNNNTCC 2 cut(s) 672, 704
AluBI AGCT 7 cut(s) 205, 221, 397, 424, 467, 473, 533
AluI AGCT 7 cut(s) 205, 221, 397, 424, 467, 473, 533
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 2 cut(s) 151, 720
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 551
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 4 cut(s) 267, 498, 593, 668
AvaII GGWCC 1 cut(s) 551
BanI GGYRCC 1 cut(s) 451
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 164
BccI CCATC 3 cut(s) 263, 670, 782
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 2 cut(s) 187, 654
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 140
BfoI RGCGCY 1 cut(s) 455
BfuAI ACCTGC 1 cut(s) 571
Bme1390I CCNGG 3 cut(s) 187, 549, 654
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 4 cut(s) 61, 453, 631, 763
BmrFI CCNGG 3 cut(s) 187, 549, 654
BmsI GCATC 3 cut(s) 310, 335, 720
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 2 cut(s) 453, 525
BpuEI CTTGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 452
BsaJI CCNNGG 3 cut(s) 27, 55, 621
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 672, 702
Bsc4I CCNNNNNNNGG 3 cut(s) 548, 626, 653
Bse1I ACTGG 3 cut(s) 300, 783, 797
BseBI CCWGG 2 cut(s) 187, 654
BseDI CCNNGG 3 cut(s) 27, 55, 621
BseLI CCNNNNNNNGG 3 cut(s) 548, 626, 653
BseMII CTCAG 2 cut(s) 213, 762
BseNI ACTGG 3 cut(s) 300, 783, 797
BseRI GAGGAG 4 cut(s) 52, 86, 525, 528
BseYI CCCAGC 1 cut(s) 69
BsgI GTGCAG 3 cut(s) 215, 549, 633
BshFI GGCC 2 cut(s) 153, 722
BshNI GGYRCC 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 278, 549
BslFI GGGAC 1 cut(s) 9
BslI CCNNNNNNNGG 3 cut(s) 548, 626, 653
BsmAI GTCTC 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 2 cut(s) 153, 722
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 272, 751
Bsp19I CCATGG 1 cut(s) 621
BspACI CCGC 7 cut(s) 39, 52, 66, 83, 336, 573, 640
BspANI GGCC 2 cut(s) 153, 722
BspCNI CTCAG 2 cut(s) 214, 763
BspLI GGNNCC 4 cut(s) 61, 453, 631, 763
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 451
BsrI ACTGG 3 cut(s) 300, 783, 797
BssECI CCNNGG 3 cut(s) 27, 55, 621
BssMI GATC 2 cut(s) 272, 751
BssNI GRCGYC 1 cut(s) 452
BssT1I CCWWGG 2 cut(s) 27, 621
Bst2UI CCWGG 2 cut(s) 187, 654
Bst4CI ACNGT 3 cut(s) 16, 166, 766
BstACI GRCGYC 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 34, 198
BstDEI CTNAG 2 cut(s) 222, 771
BstDSI CCRYGG 1 cut(s) 621
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 2 cut(s) 275, 754
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 2 cut(s) 272, 751
BstNI CCWGG 2 cut(s) 187, 654
BstSCI CCNGG 3 cut(s) 185, 547, 652
BstSFI CTRYAG 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 288
BsuRI GGCC 2 cut(s) 153, 722
BtgI CCRYGG 1 cut(s) 621
BtsIMutI CAGTG 3 cut(s) 12, 608, 808
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 2 cut(s) 34, 198
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 551
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 2 cut(s) 242, 830
CviAII CATG 4 cut(s) 343, 502, 622, 724
CviQI GTAC 2 cut(s) 242, 830
DdeI CTNAG 2 cut(s) 222, 771
DinI GGCGCC 1 cut(s) 453
DpnI GATC 2 cut(s) 274, 753
DpnII GATC 2 cut(s) 272, 751
DraIII CACNNNGTG 1 cut(s) 656
EciI GGCGGA 2 cut(s) 28, 55
Eco130I CCWWGG 2 cut(s) 27, 621
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 140, 285
EcoRII CCWGG 2 cut(s) 185, 652
EcoT14I CCWWGG 2 cut(s) 27, 621
EgeI GGCGCC 1 cut(s) 453
EheI GGCGCC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 27, 621
FaeI CATG 4 cut(s) 346, 505, 625, 727
FaiI YATR 6 cut(s) 248, 344, 503, 623, 725, 827
FaqI GGGAC 1 cut(s) 9
FatI CATG 4 cut(s) 342, 501, 621, 723
FauI CCCGC 2 cut(s) 59, 580
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 807
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 453
GsaI CCCAGC 1 cut(s) 73
GsuI CTGGAG 2 cut(s) 453, 525
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 153, 722
HapII CCGG 2 cut(s) 278, 549
HhaI GCGC 1 cut(s) 454
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 4 cut(s) 346, 505, 625, 727
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HinfI GANTC 2 cut(s) 293, 377
HpaII CCGG 2 cut(s) 278, 549
HphI GGTGA 4 cut(s) 267, 498, 593, 668
Hpy166II GTNNAC 3 cut(s) 554, 659, 808
Hpy188I TCNGA 4 cut(s) 159, 265, 358, 693
Hpy188III TCNNGA 4 cut(s) 169, 317, 381, 759
Hpy8I GTNNAC 3 cut(s) 554, 659, 808
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 5 cut(s) 19, 112, 164, 184, 275
HpyCH4III ACNGT 3 cut(s) 16, 166, 766
HpyCH4V TGCA 8 cut(s) 142, 196, 218, 323, 566, 581, 650, 711
HpyF3I CTNAG 2 cut(s) 222, 771
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 4 cut(s) 346, 505, 625, 727
HspAI GCGC 1 cut(s) 452
KasI GGCGCC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 2 cut(s) 272, 751
LmnI GCTCC 5 cut(s) 41, 65, 394, 472, 538
LweI GCATC 3 cut(s) 310, 335, 720
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 46, 504
MalI GATC 2 cut(s) 274, 753
MboI GATC 2 cut(s) 272, 751
MboII GAAGA 5 cut(s) 171, 174, 278, 288, 797
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 4 cut(s) 235, 330, 728, 741
Mly113I GGCGCC 1 cut(s) 452
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 658
MseI TTAA 2 cut(s) 207, 366
MslI CAYNNNNRTG 1 cut(s) 613
MspA1I CMGCKG 2 cut(s) 221, 642
MspI CCGG 2 cut(s) 278, 549
MspR9I CCNGG 3 cut(s) 187, 549, 654
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 2 cut(s) 187, 654
NarI GGCGCC 1 cut(s) 452
NciI CCSGG 1 cut(s) 549
NcoI CCATGG 1 cut(s) 621
NdeII GATC 2 cut(s) 272, 751
NlaIII CATG 4 cut(s) 346, 505, 625, 727
NlaIV GGNNCC 4 cut(s) 61, 453, 631, 763
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 504
PaqCI CACCTGC 1 cut(s) 571
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 185
PleI GAGTC 1 cut(s) 301
PluTI GGCGCC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 185, 652
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 2 cut(s) 185, 652
PspN4I GGNNCC 4 cut(s) 61, 453, 631, 763
PspPI GGNCC 1 cut(s) 551
PstI CTGCAG 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 2 cut(s) 243, 831
RsaNI GTAC 2 cut(s) 242, 830
RseI CAYNNNNRTG 1 cut(s) 613
SaqAI TTAA 2 cut(s) 207, 366
Sau3AI GATC 2 cut(s) 272, 751
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 3 cut(s) 187, 549, 654
SduI GDGCHC 1 cut(s) 164
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 3 cut(s) 310, 335, 720
SfcI CTRYAG 1 cut(s) 140
SfoI GGCGCC 1 cut(s) 453
SinI GGWCC 1 cut(s) 551
SmiMI CAYNNNNRTG 1 cut(s) 613
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 4 cut(s) 235, 330, 728, 741
SsiI CCGC 7 cut(s) 39, 52, 66, 83, 336, 573, 640
SspDI GGCGCC 1 cut(s) 451
SspI AATATT 2 cut(s) 313, 494
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 185, 547, 652
StyI CCWWGG 2 cut(s) 27, 621
TaaI ACNGT 3 cut(s) 16, 166, 766
TaqI TCGA 1 cut(s) 380
TasI AATT 4 cut(s) 235, 330, 728, 741
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 2 cut(s) 207, 366
Tru9I TTAA 2 cut(s) 207, 366
TscAI CASTG 3 cut(s) 19, 615, 808
TseFI GTSAC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 4 cut(s) 331, 419, 740, 749
TspRI CASTG 3 cut(s) 19, 615, 808
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 807
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.