Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008330

Physical Location & Seq

Genomic Coordinates Forward (+)

4692599 .. 4695150

Gene Structure 2552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1057500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGGCTTCCTTCACTGTCCCCTGTCCCAAGGCGTGCGCCGCCCCCGTTACCCGCCTCAGCTCCGCCGCCCAGCCGTCGCAGCCGCAGCAGCACCTCCTGTCCTTCCACAACTGCCTCAATCGCTGCCCCATTCCCTCTCTGCAGATTTCTGGCCTTCAGAGCTCCGTCAGGAAGCAATCCTCTTCCTGGAAAGTGCAGGCACAGCTTAATGAGGTTACTGCTGAGAAATCCTCAAATTCTGTACCCATAAACAACAAGAAGTCTGAAGATGGATCACCGGAAGGGAAAGACGAGTCTTCCAGTCAAACCAATATTCCAGATGCAGCATCAATCTCCGCATTCATGGCTCAAGTGTCAAACCTTGTTAAGTTAGTTGATTCGAGAGACATTGTGGAGCTGGAATTGAAGCAACTAGATTTAGAGCTTGTGATTAGAAAAAAAGAAGCGTTGGCGCCACCACCACCAGCTCCCGTTTCGCAACAACCAGCACACTATTATTCCATACCACCACCTCAACAAGCACCGGCACCAGCACCAGCATCAGCTCCTGCTCCAGCACCCGGTCCAGCAGCACCTGCATTACCCGCCCCTGCAAAGACAAGCACATCATCTCACCCTCCACTGAAATGCCCCATGGCTGGAACCCTTTACCGCTGTCCTGCACCTGGTGAACCACCTTTTGTTAAGGTTGGGGATAAAGTTCAGAAAGGTCAAGTTATTTGCATCATTGAGGCCATGAAATTGATGAACGAAATTGAGGCTGATCAATCTGGAACCGTTGCTGAGATACTGGTTGAAGATGCTAAACCAGTGAGCGTAGACACGCCTCTTTTTGTCATAGTACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212

Pfam Domains

Protein Families

Biotin_lipoyl

Protein Analysis

283

Amino Acids

29.7

Weight (kDa)

6.43

Isoelectric Point (pI)

72.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	208 - 280	8.9e-24	Biotin-requiring enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008330)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15530
fragaria_vesca	FvH4_6g47090 FvH4_6g47090 FvH4_6g47090 FvH4_6g47090
malus_domestica	MD09G1064200.v1.1 MD10G1103400.v1.1 MD17G1057500.v1.1
prunus_persica	Prupe.3G256800_v2.0.a1
pyrus_communis	pycom111g05390 pycom17g05760
rosa_chinensis	RchiOBHm_Chr2g0166141
rosa_laevigata	RLG00000021589
rosa_multiflora	Rmu_co8361143.1_g000001 Rmu_sc0006218.1_g000029
rosa_roxburghii	Rroxscaffold_2G00085170
rosa_rugosa	Rorug02G0520700 Rorug02G0520700
rosa_samantha	Rh2AG586700 Rh2BG598500 Rh2CG569200 Rh2DG608400
rosa_wichuraiana	Rw2G048910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	583
Acc36I	ACCTGC	1 cut(s)	583
AccB1I	GGYRCC	2 cut(s)	451, 526
AccI	GTMKAC	1 cut(s)	819
AciI	CCGC	8 cut(s)	39, 52, 63, 66, 83, 336, 585, 652
AclWI	GGATC	1 cut(s)	280
AcsI	RAATTY	1 cut(s)	235
AcuI	CTGAAG	2 cut(s)	140, 285
AcyI	GRCGYC	1 cut(s)	452
AdeI	CACNNNGTG	1 cut(s)	668
AfaI	GTAC	2 cut(s)	243, 843
AfiI	CCNNNNNNNGG	4 cut(s)	186, 560, 638, 665
AgsI	TTSAA	2 cut(s)	406, 797
AjnI	CCWGG	2 cut(s)	185, 664
AluBI	AGCT	7 cut(s)	60, 162, 205, 397, 424, 467, 545
AluI	AGCT	7 cut(s)	60, 162, 205, 397, 424, 467, 545
Alw21I	GWGCWC	1 cut(s)	164
Alw26I	GTCTC	1 cut(s)	378
AlwI	GGATC	1 cut(s)	280
AlwNI	CAGNNNCTG	2 cut(s)	548, 575
AoxI	GGCC	2 cut(s)	151, 732
ApeKI	GCWGC	6 cut(s)	79, 85, 88, 123, 323, 569
ApoI	RAATTY	1 cut(s)	235
AspLEI	GCGC	2 cut(s)	38, 454
AspS9I	GGNCC	1 cut(s)	563
AsuC2I	CCSGG	1 cut(s)	561
AsuHPI	GGTGA	3 cut(s)	267, 605, 680
AvaII	GGWCC	1 cut(s)	563
BanI	GGYRCC	2 cut(s)	451, 526
BanII	GRGCYC	1 cut(s)	164
BbsI	GAAGAC	1 cut(s)	288
Bbv12I	GWGCWC	1 cut(s)	164
BbvCI	CCTCAGC	1 cut(s)	56
BbvI	GCAGC	6 cut(s)	91, 97, 100, 110, 335, 581
BccI	CCATC	1 cut(s)	263
BceAI	ACGGC	1 cut(s)	58
BciT130I	CCWGG	2 cut(s)	187, 666
BclI	TGATCA	1 cut(s)	763
BcnI	CCSGG	1 cut(s)	561
BcoDI	GTCTC	1 cut(s)	378
BfaI	CTAG	1 cut(s)	413
BfmI	CTRYAG	1 cut(s)	140
BfoI	RGCGCY	1 cut(s)	455
BfuAI	ACCTGC	1 cut(s)	583
BisI	GCNGC	9 cut(s)	39, 66, 80, 83, 86, 89, 124, 324, 570
BlsI	GCNGC	9 cut(s)	40, 67, 81, 84, 87, 90, 125, 325, 571
Bme1390I	CCNGG	3 cut(s)	187, 561, 666
Bme18I	GGWCC	1 cut(s)	563
BmgT120I	GGNCC	1 cut(s)	563
BmiI	GGNNCC	4 cut(s)	453, 528, 643, 775
BmrFI	CCNGG	3 cut(s)	187, 561, 666
BmsI	GCATC	5 cut(s)	310, 335, 548, 732, 790
BpiI	GAAGAC	1 cut(s)	288
BplI	GAGNNNNNCTC	2 cut(s)	215, 247
BpmI	CTGGAG	1 cut(s)	537
Bpu10I	CCTNAGC	1 cut(s)	56
BpuEI	CTTGAG	1 cut(s)	333
BpuMI	CCSGG	1 cut(s)	561
BsaHI	GRCGYC	1 cut(s)	452
BsaJI	CCNNGG	2 cut(s)	27, 633
BsaWI	WCCGGW	1 cut(s)	277
Bsc4I	CCNNNNNNNGG	4 cut(s)	186, 560, 638, 665
Bse118I	RCCGGY	1 cut(s)	523
Bse1I	ACTGG	3 cut(s)	300, 795, 809
BseBI	CCWGG	2 cut(s)	187, 666
BseDI	CCNNGG	2 cut(s)	27, 633
BseLI	CCNNNNNNNGG	4 cut(s)	186, 560, 638, 665
BseMII	CTCAG	3 cut(s)	70, 213, 774
BseNI	ACTGG	3 cut(s)	300, 795, 809
BseXI	GCAGC	6 cut(s)	91, 97, 100, 110, 335, 581
BseYI	CCCAGC	1 cut(s)	69
BsgI	GTGCAG	2 cut(s)	215, 645
BshFI	GGCC	2 cut(s)	153, 734
BshNI	GGYRCC	2 cut(s)	451, 526
BsiHKAI	GWGCWC	1 cut(s)	164
BsiSI	CCGG	3 cut(s)	278, 524, 561
BslFI	GGGAC	2 cut(s)	2, 9
BslI	CCNNNNNNNGG	4 cut(s)	186, 560, 638, 665
BsmAI	GTCTC	1 cut(s)	378
BsmFI	GGGAC	2 cut(s)	2, 9
BsmI	GAATGC	1 cut(s)	338
BsnI	GGCC	2 cut(s)	153, 734
Bsp1286I	GDGCHC	1 cut(s)	164
Bsp143I	GATC	2 cut(s)	272, 763
Bsp19I	CCATGG	1 cut(s)	633
BspACI	CCGC	8 cut(s)	39, 52, 63, 66, 83, 336, 585, 652
BspANI	GGCC	2 cut(s)	153, 734
BspCNI	CTCAG	3 cut(s)	69, 214, 775
BspLI	GGNNCC	4 cut(s)	453, 528, 643, 775
BspMAI	CTGCAG	1 cut(s)	144
BspMI	ACCTGC	1 cut(s)	583
BspPI	GGATC	1 cut(s)	280
BspT107I	GGYRCC	2 cut(s)	451, 526
BsrFI	RCCGGY	1 cut(s)	523
BsrI	ACTGG	3 cut(s)	300, 795, 809
BssAI	RCCGGY	1 cut(s)	523
BssECI	CCNNGG	2 cut(s)	27, 633
BssMI	GATC	2 cut(s)	272, 763
BssNI	GRCGYC	1 cut(s)	452
BssT1I	CCWWGG	2 cut(s)	27, 633
Bst2UI	CCWGG	2 cut(s)	187, 666
Bst4CI	ACNGT	2 cut(s)	16, 778
Bst6I	CTCTTC	1 cut(s)	187
BstACI	GRCGYC	1 cut(s)	452
BstAPI	GCANNNNNTGC	1 cut(s)	575
BstC8I	GCNNGC	2 cut(s)	34, 198
BstDEI	CTNAG	3 cut(s)	56, 222, 783
BstDSI	CCRYGG	1 cut(s)	633
BstH2I	RGCGCY	1 cut(s)	455
BstHHI	GCGC	2 cut(s)	38, 454
BstKTI	GATC	2 cut(s)	275, 766
BstMAI	GTCTC	1 cut(s)	378
BstMBI	GATC	2 cut(s)	272, 763
BstNI	CCWGG	2 cut(s)	187, 666
BstSCI	CCNGG	3 cut(s)	185, 559, 664
BstSFI	CTRYAG	1 cut(s)	140
BstV1I	GCAGC	6 cut(s)	91, 97, 100, 110, 335, 581
BstV2I	GAAGAC	1 cut(s)	288
BsuRI	GGCC	2 cut(s)	153, 734
BtgI	CCRYGG	1 cut(s)	633
BtsIMutI	CAGTG	3 cut(s)	12, 620, 816
BveI	ACCTGC	1 cut(s)	583
Cac8I	GCNNGC	2 cut(s)	34, 198
CaiI	CAGNNNCTG	2 cut(s)	548, 575
CfoI	GCGC	2 cut(s)	38, 454
Cfr10I	RCCGGY	1 cut(s)	523
Cfr13I	GGNCC	1 cut(s)	563
CsiI	ACCWGGT	1 cut(s)	664
Csp6I	GTAC	2 cut(s)	242, 842
CviAII	CATG	4 cut(s)	343, 634, 736, 846
CviQI	GTAC	2 cut(s)	242, 842
DdeI	CTNAG	3 cut(s)	56, 222, 783
DinI	GGCGCC	1 cut(s)	453
DpnI	GATC	2 cut(s)	274, 765
DpnII	GATC	2 cut(s)	272, 763
DraIII	CACNNNGTG	1 cut(s)	668
Eam1104I	CTCTTC	1 cut(s)	187
EarI	CTCTTC	1 cut(s)	187
EciI	GGCGGA	1 cut(s)	52
Ecl136II	GAGCTC	1 cut(s)	162
Eco130I	CCWWGG	2 cut(s)	27, 633
Eco24I	GRGCYC	1 cut(s)	164
Eco47I	GGWCC	1 cut(s)	563
Eco53kI	GAGCTC	1 cut(s)	162
Eco57I	CTGAAG	2 cut(s)	140, 285
EcoICRI	GAGCTC	1 cut(s)	162
EcoRII	CCWGG	2 cut(s)	185, 664
EcoT14I	CCWWGG	2 cut(s)	27, 633
EcoT38I	GRGCYC	1 cut(s)	164
EgeI	GGCGCC	1 cut(s)	453
EheI	GGCGCC	1 cut(s)	453
ErhI	CCWWGG	2 cut(s)	27, 633
FaeI	CATG	4 cut(s)	346, 637, 739, 849
FaiI	YATR	7 cut(s)	248, 344, 503, 635, 737, 839, 847
FaqI	GGGAC	2 cut(s)	2, 9
FatI	CATG	4 cut(s)	342, 633, 735, 845
FauI	CCCGC	2 cut(s)	59, 592
FbaI	TGATCA	1 cut(s)	763
FblI	GTMKAC	1 cut(s)	819
Fnu4HI	GCNGC	9 cut(s)	39, 66, 80, 83, 86, 89, 124, 324, 570
FriOI	GRGCYC	1 cut(s)	164
Fsp4HI	GCNGC	9 cut(s)	39, 66, 80, 83, 86, 89, 124, 324, 570
FspBI	CTAG	1 cut(s)	413
GlaI	GCGC	2 cut(s)	37, 453
GluI	GCNGC	9 cut(s)	39, 66, 80, 83, 86, 89, 124, 324, 570
GsaI	CCCAGC	1 cut(s)	73
GsuI	CTGGAG	1 cut(s)	537
HaeII	RGCGCY	1 cut(s)	455
HaeIII	GGCC	2 cut(s)	153, 734
HapII	CCGG	3 cut(s)	278, 524, 561
HhaI	GCGC	2 cut(s)	38, 454
Hin1I	GRCGYC	1 cut(s)	452
Hin1II	CATG	4 cut(s)	346, 637, 739, 849
Hin6I	GCGC	2 cut(s)	36, 452
HinP1I	GCGC	2 cut(s)	36, 452
HinfI	GANTC	2 cut(s)	293, 377
HpaII	CCGG	3 cut(s)	278, 524, 561
HphI	GGTGA	3 cut(s)	267, 605, 680
Hpy166II	GTNNAC	2 cut(s)	671, 820
Hpy188I	TCNGA	3 cut(s)	159, 265, 705
Hpy188III	TCNNGA	4 cut(s)	169, 317, 381, 771
Hpy8I	GTNNAC	2 cut(s)	671, 820
Hpy99I	CGWCG	1 cut(s)	79
HpyAV	CCTTC	4 cut(s)	19, 112, 164, 275
HpyCH4III	ACNGT	2 cut(s)	16, 778
HpyCH4V	TGCA	7 cut(s)	142, 196, 323, 578, 593, 662, 723
HpyF3I	CTNAG	3 cut(s)	56, 222, 783
Hsp92I	GRCGYC	1 cut(s)	452
Hsp92II	CATG	4 cut(s)	346, 637, 739, 849
HspAI	GCGC	2 cut(s)	36, 452
KasI	GGCGCC	1 cut(s)	451
Ksp22I	TGATCA	1 cut(s)	763
Kzo9I	GATC	2 cut(s)	272, 763
LmnI	GCTCC	6 cut(s)	65, 167, 394, 472, 550, 556
Lsp1109I	GCAGC	6 cut(s)	91, 97, 100, 110, 335, 581
LweI	GCATC	5 cut(s)	310, 335, 548, 732, 790
MabI	ACCWGGT	1 cut(s)	664
MaeI	CTAG	1 cut(s)	413
MaeIII	GTNAC	2 cut(s)	46, 214
MalI	GATC	2 cut(s)	274, 765
MboI	GATC	2 cut(s)	272, 763
MboII	GAAGA	4 cut(s)	174, 278, 288, 809
MhlI	GDGCHC	1 cut(s)	164
MluCI	AATT	4 cut(s)	235, 401, 740, 753
Mly113I	GGCGCC	1 cut(s)	452
MlyI	GAGTC	1 cut(s)	302
MseI	TTAA	3 cut(s)	207, 366, 684
MslI	CAYNNNNRTG	1 cut(s)	625
MspA1I	CMGCKG	1 cut(s)	654
MspI	CCGG	3 cut(s)	278, 524, 561
MspR9I	CCNGG	3 cut(s)	187, 561, 666
Mva1269I	GAATGC	1 cut(s)	338
MvaI	CCWGG	2 cut(s)	187, 666
NarI	GGCGCC	1 cut(s)	452
NciI	CCSGG	1 cut(s)	561
NcoI	CCATGG	1 cut(s)	633
NdeII	GATC	2 cut(s)	272, 763
NlaIII	CATG	4 cut(s)	346, 637, 739, 849
NlaIV	GGNNCC	4 cut(s)	453, 528, 643, 775
PaqCI	CACCTGC	1 cut(s)	583
PctI	GAATGC	1 cut(s)	338
PfeI	GAWTC	1 cut(s)	377
PfoI	TCCNGGA	1 cut(s)	185
PkrI	GCNGC	9 cut(s)	40, 67, 81, 84, 87, 90, 125, 325, 571
PleI	GAGTC	1 cut(s)	301
PluTI	GGCGCC	1 cut(s)	455
PpsI	GAGTC	1 cut(s)	301
Psp124BI	GAGCTC	1 cut(s)	164
Psp6I	CCWGG	2 cut(s)	185, 664
PspFI	CCCAGC	1 cut(s)	69
PspGI	CCWGG	2 cut(s)	185, 664
PspN4I	GGNNCC	4 cut(s)	453, 528, 643, 775
PspPI	GGNCC	1 cut(s)	563
PstI	CTGCAG	1 cut(s)	144
PstNI	CAGNNNCTG	2 cut(s)	548, 575
RsaI	GTAC	2 cut(s)	243, 843
RsaNI	GTAC	2 cut(s)	242, 842
RseI	CAYNNNNRTG	1 cut(s)	625
SacI	GAGCTC	1 cut(s)	164
SaqAI	TTAA	3 cut(s)	207, 366, 684
SatI	GCNGC	9 cut(s)	39, 66, 80, 83, 86, 89, 124, 324, 570
Sau3AI	GATC	2 cut(s)	272, 763
Sau96I	GGNCC	1 cut(s)	563
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	3 cut(s)	187, 561, 666
SduI	GDGCHC	1 cut(s)	164
SexAI	ACCWGGT	1 cut(s)	664
SfaNI	GCATC	5 cut(s)	310, 335, 548, 732, 790
SfcI	CTRYAG	1 cut(s)	140
SfoI	GGCGCC	1 cut(s)	453
SinI	GGWCC	1 cut(s)	563
SmiMI	CAYNNNNRTG	1 cut(s)	625
SmlI	CTYRAG	1 cut(s)	348
SmoI	CTYRAG	1 cut(s)	348
Sse9I	AATT	4 cut(s)	235, 401, 740, 753
SsiI	CCGC	8 cut(s)	39, 52, 63, 66, 83, 336, 585, 652
SspDI	GGCGCC	1 cut(s)	451
SspI	AATATT	1 cut(s)	313
SspMI	CTAG	1 cut(s)	413
SstI	GAGCTC	1 cut(s)	164
StyD4I	CCNGG	3 cut(s)	185, 559, 664
StyI	CCWWGG	2 cut(s)	27, 633
TaaI	ACNGT	2 cut(s)	16, 778
TaqI	TCGA	1 cut(s)	380
TasI	AATT	4 cut(s)	235, 401, 740, 753
TauI	GCSGC	3 cut(s)	41, 68, 85
TfiI	GAWTC	1 cut(s)	377
Tru1I	TTAA	3 cut(s)	207, 366, 684
Tru9I	TTAA	3 cut(s)	207, 366, 684
TscAI	CASTG	3 cut(s)	19, 627, 816
TseI	GCWGC	6 cut(s)	79, 85, 88, 123, 323, 569
TspDTI	ATGAA	3 cut(s)	331, 752, 761
TspGWI	ACGGA	1 cut(s)	154
TspRI	CASTG	3 cut(s)	19, 627, 816
VpaK11BI	GGWCC	1 cut(s)	563
XapI	RAATTY	1 cut(s)	235
XmiI	GTMKAC	1 cut(s)	819
XspI	CTAG	1 cut(s)	413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.