AT5G20700

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010874
Physical Location & Seq
Reverse (-)
7005667 .. 7007502
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20700.1

Sequence Viewer

Length: 747 bp
ATGCTTACTAAAAGAACCCATCCCATGATCGGAAAGATATCGGAGCTCCTCGTCGGTGTCAACCGATCCACGGCGGCTCCTTTCTTCGACGTCTTGATGACGAGCCCTAAAAGCCCACTGGATTTCAAGATTCTCCCTCAGATATCTCAAAGAAACAGCTCAAAGAGATTCTACGATGACAATCTTGGTGGCTCTGTTGGTCTAGGGATTGTAGCCGCGCTCGAGAACTCAAACACTCGTCGAATCACGAGCGTTTGTAGATCGGAACCGAACCAACCCGGCCGGTCTGATCCGGTTCAGTTCATGAGCCATGGTGGAAGCACGGACGGAGAAGACGAGGAGATGTTCATAATGGACGAGGAGGATTACACGTTGGTGACATGCCACCATGGACCAAGTGGATCTTGTAATACAAGGGTTTATGACAAAGATGGGTTTGAGTGTTTCTCAAGTAAGATCAACGATGATCGTCGGGAAAGACTTTTCGTGGTCGATGTCGTTACGGAATCTCCAGAGAATTCGCCAGAGTTTCAGGGTTTGGGTTTCCTCAATTCATGTTACTTGTGCAGGAAGAAACTTCATGGTCAAGACATATTTATTTACAGAGGAGAAAAAGCATTTTGCAGCACAGAGTGTCGATCGAGTCATATAGCAAATGATGAAAGGAAAGAGAGATGTAGATCAAAATTCTCAACCTCTCCTTACACCGCCGGCCAAATTTTCTCCACCGGAGTTCTAGTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

248

Amino Acids

27.74

Weight (kDa)

6.1

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 174 - 223 1.6e-21 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19200 AT1G19200 AT1G74940 AT5G20700
fragaria_vesca FvH4_1g09090
malus_domestica MD02G1096300.v1.1 MD15G1220800.v1.1
prunus_persica Prupe.7G194600_v2.0.a1
pyrus_communis pycom02g07610 pycom15g19560
rosa_chinensis RchiOBHm_Chr2g0095501
rosa_laevigata RLG00000016573
rosa_multiflora Rmu_sc0006076.1_g000006
rosa_roxburghii Rroxscaffold_2G00146250
rosa_rugosa Rorug02G0051700
rosa_samantha Rh2AG099700 Rh2BG101000 Rh2CG103200 Rh2DG100600
rosa_wichuraiana Rw2G007560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 74, 216, 708
AclWI GGATC 3 cut(s) 60, 284, 409
AcoI YGGCCR 2 cut(s) 280, 712
AcsI RAATTY 3 cut(s) 517, 686, 717
AcyI GRCGYC 1 cut(s) 90
AdeI CACNNNGTG 1 cut(s) 633
AfiI CCNNNNNNNGG 2 cut(s) 29, 70
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 1 cut(s) 127
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 2 cut(s) 46, 159
AluI AGCT 2 cut(s) 46, 159
Alw21I GWGCWC 1 cut(s) 48
AlwI GGATC 3 cut(s) 60, 284, 409
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 2 cut(s) 280, 712
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 3 cut(s) 517, 686, 717
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 392
BanII GRGCYC 2 cut(s) 48, 107
BauI CACGAG 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 636
BccI CCATC 2 cut(s) 27, 425
BceAI ACGGC 1 cut(s) 87
BcnI CCSGG 1 cut(s) 279
BfaI CTAG 2 cut(s) 203, 737
BisI GCNGC 3 cut(s) 75, 216, 625
BlsI GCNGC 3 cut(s) 76, 217, 626
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 2 cut(s) 78, 267
BmrFI CCNGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 339
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 279
BsaBI GATNNNNATC 2 cut(s) 180, 679
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 69, 310, 388
BsaWI WCCGGW 2 cut(s) 292, 728
BsaXI ACNNNNNCTCC 5 cut(s) 61, 91, 493, 523, 723
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 70
Bse118I RCCGGY 2 cut(s) 282, 710
Bse1I ACTGG 1 cut(s) 123
Bse8I GATNNNNATC 2 cut(s) 180, 679
BseDI CCNNGG 3 cut(s) 69, 310, 388
BseGI GGATG 1 cut(s) 19
BseJI GATNNNNATC 2 cut(s) 180, 679
BseLI CCNNNNNNNGG 2 cut(s) 29, 70
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 4 cut(s) 38, 353, 374, 621
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 1 cut(s) 636
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 2 cut(s) 283, 641
BshFI GGCC 2 cut(s) 282, 714
BsiEI CGRYCG 2 cut(s) 283, 641
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 5 cut(s) 279, 283, 293, 711, 729
BslI CCNNNNNNNGG 2 cut(s) 29, 70
BsnI GGCC 2 cut(s) 282, 714
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 48, 107
Bsp143I GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
Bsp19I CCATGG 2 cut(s) 310, 388
BspACI CCGC 3 cut(s) 74, 216, 708
BspANI GGCC 2 cut(s) 282, 714
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 2 cut(s) 78, 267
BspPI GGATC 3 cut(s) 60, 284, 409
BsrFI RCCGGY 2 cut(s) 282, 710
BsrI ACTGG 1 cut(s) 123
BssAI RCCGGY 2 cut(s) 282, 710
BssECI CCNNGG 3 cut(s) 69, 310, 388
BssMI GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 2 cut(s) 310, 388
Bst2BI CACGAG 1 cut(s) 247
BstACI GRCGYC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 712
BstDEI CTNAG 2 cut(s) 138, 744
BstDSI CCRYGG 3 cut(s) 69, 310, 388
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 9 cut(s) 30, 68, 263, 292, 404, 459, 469, 641, 683
BstMBI GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
BstMCI CGRYCG 2 cut(s) 283, 641
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 1 cut(s) 277
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 636
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 2 cut(s) 282, 714
BtgI CCRYGG 3 cut(s) 69, 310, 388
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 712
CciI TCATGA 1 cut(s) 303
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 2 cut(s) 282, 710
Cfr13I GGNCC 1 cut(s) 392
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 7 cut(s) 25, 304, 311, 381, 389, 555, 581
DdeI CTNAG 2 cut(s) 138, 744
DpnI GATC 9 cut(s) 29, 67, 262, 291, 403, 458, 468, 640, 682
DpnII GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
DraIII CACNNNGTG 1 cut(s) 633
EaeI YGGCCR 2 cut(s) 280, 712
EagI CGGCCG 1 cut(s) 280
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 310, 388
Eco24I GRGCYC 2 cut(s) 48, 107
Eco32I GATATC 2 cut(s) 39, 144
Eco47I GGWCC 1 cut(s) 392
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 517
EcoRV GATATC 2 cut(s) 39, 144
EcoT14I CCWWGG 2 cut(s) 310, 388
EcoT38I GRGCYC 2 cut(s) 48, 107
ErhI CCWWGG 2 cut(s) 310, 388
FaeI CATG 7 cut(s) 28, 307, 314, 384, 392, 558, 584
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 7 cut(s) 24, 303, 310, 380, 388, 554, 580
Fnu4HI GCNGC 3 cut(s) 75, 216, 625
FokI GGATG 1 cut(s) 6
FriOI GRGCYC 2 cut(s) 48, 107
Fsp4HI GCNGC 3 cut(s) 75, 216, 625
FspBI CTAG 2 cut(s) 203, 737
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 75, 216, 625
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 2 cut(s) 282, 714
HapII CCGG 5 cut(s) 279, 283, 293, 711, 729
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 7 cut(s) 28, 307, 314, 384, 392, 558, 584
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 5 cut(s) 130, 168, 243, 506, 643
HpaII CCGG 5 cut(s) 279, 283, 293, 711, 729
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 5 cut(s) 32, 43, 141, 265, 289
Hpy188III TCNNGA 8 cut(s) 94, 127, 223, 247, 304, 473, 512, 587
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 4 cut(s) 56, 92, 243, 474
HpyCH4IV ACGT 2 cut(s) 90, 371
HpyCH4V TGCA 2 cut(s) 567, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 2 cut(s) 138, 744
HpySE526I ACGT 2 cut(s) 90, 371
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 7 cut(s) 28, 307, 314, 384, 392, 558, 584
HspAI GCGC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
Kzo9I GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
LmnI GCTCC 3 cut(s) 43, 51, 82
Lsp1109I GCAGC 1 cut(s) 636
MaeI CTAG 2 cut(s) 203, 737
MaeII ACGT 2 cut(s) 90, 371
MaeIII GTNAC 4 cut(s) 376, 499, 557, 739
MalI GATC 9 cut(s) 29, 67, 262, 291, 403, 458, 468, 640, 682
MboI GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
MboII GAAGA 3 cut(s) 76, 344, 583
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 48, 107
MluCI AATT 4 cut(s) 517, 550, 686, 717
MlyI GAGTC 1 cut(s) 652
MnlI CCTC 8 cut(s) 59, 147, 331, 352, 355, 557, 599, 706
MroNI GCCGGC 1 cut(s) 710
MslI CAYNNNNRTG 1 cut(s) 374
MspI CCGG 5 cut(s) 279, 283, 293, 711, 729
MspR9I CCNGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 111
NaeI GCCGGC 1 cut(s) 712
NciI CCSGG 1 cut(s) 279
NcoI CCATGG 2 cut(s) 310, 388
NdeII GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
NgoMIV GCCGGC 1 cut(s) 710
NlaIII CATG 7 cut(s) 28, 307, 314, 384, 392, 558, 584
NlaIV GGNNCC 2 cut(s) 78, 267
NmuCI GTSAC 2 cut(s) 376, 739
NspI RCATGY 1 cut(s) 384
OliI CACNNNNGTG 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 221
PagI TCATGA 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 712
PfeI GAWTC 4 cut(s) 130, 168, 243, 506
PkrI GCNGC 3 cut(s) 76, 217, 626
Ple19I CGATCG 1 cut(s) 641
PleI GAGTC 1 cut(s) 651
PpsI GAGTC 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 78, 267
PspPI GGNCC 1 cut(s) 392
PsuI RGATCY 1 cut(s) 401
PvuI CGATCG 1 cut(s) 641
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 48
SatI GCNGC 3 cut(s) 75, 216, 625
Sau3AI GATC 9 cut(s) 27, 65, 260, 289, 401, 456, 466, 638, 680
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 48, 107
SetI ASST 5 cut(s) 48, 93, 161, 374, 698
Sfr274I CTCGAG 1 cut(s) 221
SinI GGWCC 1 cut(s) 392
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 221, 448
SmoI CTYRAG 2 cut(s) 221, 448
Sse9I AATT 4 cut(s) 517, 550, 686, 717
SsiI CCGC 3 cut(s) 74, 216, 708
SspMI CTAG 2 cut(s) 203, 737
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 2 cut(s) 310, 388
TaiI ACGT 2 cut(s) 93, 374
TaqI TCGA 6 cut(s) 87, 222, 241, 492, 637, 641
TasI AATT 4 cut(s) 517, 550, 686, 717
TauI GCSGC 2 cut(s) 77, 218
TfiI GAWTC 4 cut(s) 130, 168, 243, 506
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 2 cut(s) 376, 739
TseI GCWGC 1 cut(s) 624
Tsp45I GTSAC 2 cut(s) 376, 739
TspDTI ATGAA 5 cut(s) 292, 337, 543, 569, 675
TspGWI ACGGA 3 cut(s) 338, 342, 518
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 3 cut(s) 517, 686, 717
XceI RCATGY 1 cut(s) 384
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XhoI CTCGAG 1 cut(s) 221
XspI CTAG 2 cut(s) 203, 737
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.