MD02G1096300.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010874
Physical Location & Seq
Reverse (-)
7613889 .. 7615594
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1096300.v1.1.491

Sequence Viewer

Length: 885 bp
ATGTTTGAAAACAATCATCACCCACATAATTCCAAAACAAAACCATGGGCTAAGTCCCATGTCCACCCACTCCACCATCATATATTGGAGAGCCTAAAACTAAAGAGCATTCCAAATACACTCAACACTTTCACTTTTCTTATGTTGAAGTTTGCCAAGAGACCTTTCCCGATGATCGGAAAGATATCGGAACTCCTAGTTTCCGGCAACCGTGCAGGGTTTTCCGACACGGGGACAAGCCCTAGAAGTCCATTGGACCTCAAGATGCAACCTTCTCCGAGTCCTAGAGGCTTGAAGAGCTACGACGTTGGCGGTGTTGGGTTGGGCATAGTAGCTGCCTTGGAAAAATCAGCCCTTAACGGACGTGAAATATTGGCAAAATACGGCGTGTTCTGTCCGAACACGAACCGGTCCGACCCGATTCCGGTGAACTCGGGTAAAAACAGTGATGGGTTTTCGTCAACAGGTGGCCAAGAATTTGAGGAAGATAGTCTGGAGAATTATACTTACGTGACTCTTCGTGGACAAAACAAGCCATGCACGAAGGTGTACTATGACGGAGGTGACTGCGCGGGAAGTGCCATGCACCGCCCTACCGGTAACGGTGGTTTGACTATATGTGACGATGATCTTGAAAGCTCGGTTTTTCCGACGTCGGATTTTCTCAGTTGCTGCCATTTGTGCAAGAAAAGCCTTCATGGAAAGGACATATACATGTACAGAGGAGAAAAGGCATTTTGTAGCACAGAATGTCGGTCAACCCAGATAATTGCCGATGAGAGGAAAGAGCAGTGCAGATCAGAAGCTTTGAGATCAGCAGATGCTTCGAGATCATCGTGTTCGAGAGAGGAGATCGTCTTCTCCACTGGGATTCTTGTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

295

Amino Acids

32.27

Weight (kDa)

8.24

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 214 - 262 2.1e-22 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19200 AT1G19200 AT1G74940 AT5G20700
fragaria_vesca FvH4_1g09090
malus_domestica MD02G1096300.v1.1 MD15G1220800.v1.1
prunus_persica Prupe.7G194600_v2.0.a1
pyrus_communis pycom02g07610 pycom15g19560
rosa_chinensis RchiOBHm_Chr2g0095501
rosa_laevigata RLG00000016573
rosa_multiflora Rmu_sc0006076.1_g000006
rosa_roxburghii Rroxscaffold_2G00146250
rosa_rugosa Rorug02G0051700
rosa_samantha Rh2AG099700 Rh2BG101000 Rh2CG103200 Rh2DG100600
rosa_wichuraiana Rw2G007560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 656
AccII CGCG 1 cut(s) 572
AciI CCGC 3 cut(s) 312, 572, 589
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 1 cut(s) 476
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 2 cut(s) 551, 719
AfiI CCNNNNNNNGG 4 cut(s) 176, 231, 424, 780
AflIII ACRYGT 1 cut(s) 714
AgeI ACCGGT 2 cut(s) 408, 596
AgsI TTSAA 4 cut(s) 8, 148, 295, 635
AjiI CACGTC 1 cut(s) 365
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 4 cut(s) 300, 335, 639, 806
AluI AGCT 4 cut(s) 300, 335, 639, 806
Alw26I GTCTC 1 cut(s) 154
AlwNI CAGNNNCTG 1 cut(s) 672
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 2 cut(s) 335, 672
ApoI RAATTY 1 cut(s) 476
AsiGI ACCGGT 2 cut(s) 408, 596
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 2 cut(s) 256, 411
AsuHPI GGTGA 3 cut(s) 11, 439, 575
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 2 cut(s) 256, 411
BalI TGGCCA 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 2 cut(s) 322, 659
BccI CCATC 2 cut(s) 84, 443
BceAI ACGGC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 3 cut(s) 197, 243, 285
BisI GCNGC 2 cut(s) 336, 673
BlsI GCNGC 2 cut(s) 337, 674
Bme18I GGWCC 2 cut(s) 256, 411
BmeT110I CYCGRG 1 cut(s) 433
BmgBI CACGTC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 256, 411
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 2 cut(s) 255, 811
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 850
BpmI CTGGAG 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 511
BsaHI GRCGYC 1 cut(s) 653
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 44, 339
BsaWI WCCGGW 3 cut(s) 408, 424, 596
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 231, 424, 780
Bse118I RCCGGY 2 cut(s) 408, 596
Bse1I ACTGG 1 cut(s) 871
BseDI CCNNGG 2 cut(s) 44, 339
BseLI CCNNNNNNNGG 4 cut(s) 176, 231, 424, 780
BseMII CTCAG 1 cut(s) 679
BseNI ACTGG 1 cut(s) 871
BseRI GAGGAG 2 cut(s) 738, 863
BseXI GCAGC 2 cut(s) 322, 659
BsgI GTGCAG 2 cut(s) 234, 814
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 1 cut(s) 471
BshTI ACCGGT 2 cut(s) 408, 596
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 4 cut(s) 204, 409, 425, 597
BslFI GGGAC 2 cut(s) 40, 247
BslI CCNNNNNNNGG 4 cut(s) 176, 231, 424, 780
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 40, 247
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 1 cut(s) 471
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 717
Bsp143I GATC 6 cut(s) 174, 628, 797, 812, 830, 852
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 3 cut(s) 312, 572, 589
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 1 cut(s) 678
BspFNI CGCG 1 cut(s) 572
BspQI GCTCTTC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 154
BsrFI RCCGGY 2 cut(s) 408, 596
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 1 cut(s) 871
BssAI RCCGGY 2 cut(s) 408, 596
BssECI CCNNGG 2 cut(s) 44, 339
BssMI GATC 6 cut(s) 174, 628, 797, 812, 830, 852
BssNI GRCGYC 1 cut(s) 653
BssT1I CCWWGG 2 cut(s) 44, 339
Bst4CI ACNGT 3 cut(s) 212, 446, 605
Bst6I CTCTTC 2 cut(s) 290, 522
BstACI GRCGYC 1 cut(s) 653
BstAUI TGTACA 1 cut(s) 717
BstBAI YACGTR 1 cut(s) 511
BstDEI CTNAG 2 cut(s) 51, 665
BstDSI CCRYGG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 572
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 6 cut(s) 177, 631, 800, 815, 833, 855
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 6 cut(s) 174, 628, 797, 812, 830, 852
BstMWI GCNNNNNNNGC 4 cut(s) 297, 578, 681, 690
BstNSI RCATGY 1 cut(s) 718
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 2 cut(s) 322, 659
BstV2I GAAGAC 1 cut(s) 850
BsuRI GGCC 1 cut(s) 471
BtgI CCRYGG 1 cut(s) 44
BtrI CACGTC 1 cut(s) 365
BtsI GCAGTG 1 cut(s) 797
BtsIMutI CAGTG 3 cut(s) 451, 797, 864
CaiI CAGNNNCTG 1 cut(s) 672
CfoI GCGC 1 cut(s) 572
Cfr10I RCCGGY 2 cut(s) 408, 596
Cfr13I GGNCC 2 cut(s) 256, 411
CpoI CGGWCCG 1 cut(s) 411
Csp6I GTAC 2 cut(s) 550, 718
CspAI ACCGGT 2 cut(s) 408, 596
CspI CGGWCCG 1 cut(s) 411
CviAII CATG 6 cut(s) 45, 59, 537, 583, 698, 715
CviQI GTAC 2 cut(s) 550, 718
DdeI CTNAG 2 cut(s) 51, 665
DpnI GATC 6 cut(s) 176, 630, 799, 814, 832, 854
DpnII GATC 6 cut(s) 174, 628, 797, 812, 830, 852
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 290, 522
EarI CTCTTC 2 cut(s) 290, 522
Eco130I CCWWGG 2 cut(s) 44, 339
Eco31I GGTCTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 256, 411
Eco88I CYCGRG 1 cut(s) 433
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 44, 339
ErhI CCWWGG 2 cut(s) 44, 339
FaeI CATG 6 cut(s) 48, 62, 540, 586, 701, 718
FaqI GGGAC 2 cut(s) 40, 247
FatI CATG 6 cut(s) 44, 58, 536, 582, 697, 714
FauI CCCGC 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 336, 673
Fsp4HI GCNGC 2 cut(s) 336, 673
FspBI CTAG 3 cut(s) 197, 243, 285
GlaI GCGC 1 cut(s) 571
GluI GCNGC 2 cut(s) 336, 673
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 1 cut(s) 471
HapII CCGG 4 cut(s) 204, 409, 425, 597
HhaI GCGC 1 cut(s) 572
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 6 cut(s) 48, 62, 540, 586, 701, 718
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 2 cut(s) 462, 759
HindII GTYRAC 2 cut(s) 462, 759
HindIII AAGCTT 1 cut(s) 804
HinfI GANTC 4 cut(s) 280, 421, 514, 871
HpaII CCGG 4 cut(s) 204, 409, 425, 597
HphI GGTGA 3 cut(s) 11, 439, 575
Hpy166II GTNNAC 6 cut(s) 64, 430, 462, 524, 550, 759
Hpy188I TCNGA 9 cut(s) 179, 190, 226, 279, 399, 415, 651, 658, 802
Hpy188III TCNNGA 6 cut(s) 169, 262, 494, 632, 828, 843
Hpy8I GTNNAC 6 cut(s) 64, 430, 462, 524, 550, 759
Hpy99I CGWCG 3 cut(s) 308, 655, 658
HpyAV CCTTC 3 cut(s) 282, 538, 704
HpyCH4III ACNGT 3 cut(s) 212, 446, 605
HpyCH4IV ACGT 4 cut(s) 306, 364, 510, 653
HpyCH4V TGCA 6 cut(s) 215, 268, 540, 586, 684, 795
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 578, 681, 690
HpyF3I CTNAG 2 cut(s) 51, 665
HpySE526I ACGT 4 cut(s) 306, 364, 510, 653
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 6 cut(s) 48, 62, 540, 586, 701, 718
HspAI GCGC 1 cut(s) 570
Kzo9I GATC 6 cut(s) 174, 628, 797, 812, 830, 852
LguI GCTCTTC 1 cut(s) 290
LpnPI CCDG 9 cut(s) 201, 217, 422, 438, 450, 479, 610, 776, 852
Lsp1109I GCAGC 2 cut(s) 322, 659
LweI GCATC 2 cut(s) 255, 811
MaeI CTAG 3 cut(s) 197, 243, 285
MaeII ACGT 4 cut(s) 306, 364, 510, 653
MaeIII GTNAC 4 cut(s) 511, 563, 599, 620
MalI GATC 6 cut(s) 176, 630, 799, 814, 832, 854
MboI GATC 6 cut(s) 174, 628, 797, 812, 830, 852
MboII GAAGA 4 cut(s) 307, 497, 509, 850
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 4 cut(s) 28, 476, 499, 768
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 2 cut(s) 289, 508
MmeI TCCRAC 4 cut(s) 249, 438, 636, 674
MnlI CCTC 7 cut(s) 269, 281, 475, 554, 716, 774, 841
Mox20I TGGCCA 1 cut(s) 471
MscI TGGCCA 1 cut(s) 471
MseI TTAA 2 cut(s) 357, 883
MslI CAYNNNNRTG 2 cut(s) 545, 713
Msp20I TGGCCA 1 cut(s) 471
MspI CCGG 4 cut(s) 204, 409, 425, 597
Mva1269I GAATGC 1 cut(s) 108
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 4 cut(s) 297, 578, 681, 690
NcoI CCATGG 1 cut(s) 44
NdeII GATC 6 cut(s) 174, 628, 797, 812, 830, 852
NlaIII CATG 6 cut(s) 48, 62, 540, 586, 701, 718
NmuCI GTSAC 3 cut(s) 511, 563, 620
NspI RCATGY 1 cut(s) 718
OliI CACNNNNGTG 1 cut(s) 545
PciI ACATGT 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 290
PcsI WCGNNNNNNNCGW 2 cut(s) 647, 833
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 421, 871
PinAI ACCGGT 2 cut(s) 408, 596
PkrI GCNGC 2 cut(s) 337, 674
PleI GAGTC 2 cut(s) 288, 508
PpsI GAGTC 2 cut(s) 288, 508
Ppu21I YACGTR 1 cut(s) 511
PscI ACATGT 1 cut(s) 714
PspPI GGNCC 2 cut(s) 256, 411
PstNI CAGNNNCTG 1 cut(s) 672
RsaI GTAC 2 cut(s) 551, 719
RsaNI GTAC 2 cut(s) 550, 718
RseI CAYNNNNRTG 2 cut(s) 545, 713
Rsr2I CGGWCCG 1 cut(s) 411
RsrII CGGWCCG 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 2 cut(s) 357, 883
SatI GCNGC 2 cut(s) 336, 673
Sau3AI GATC 6 cut(s) 174, 628, 797, 812, 830, 852
Sau96I GGNCC 2 cut(s) 256, 411
SchI GAGTC 2 cut(s) 289, 508
SfaNI GCATC 2 cut(s) 255, 811
SinI GGWCC 2 cut(s) 256, 411
SmiMI CAYNNNNRTG 2 cut(s) 545, 713
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 4 cut(s) 28, 476, 499, 768
SsiI CCGC 3 cut(s) 312, 572, 589
SspI AATATT 1 cut(s) 372
SspMI CTAG 3 cut(s) 197, 243, 285
StyI CCWWGG 2 cut(s) 44, 339
TaaI ACNGT 3 cut(s) 212, 446, 605
TaiI ACGT 4 cut(s) 309, 367, 513, 656
TaqI TCGA 2 cut(s) 827, 842
TaqII GACCGA 1 cut(s) 744
TasI AATT 4 cut(s) 28, 476, 499, 768
TatI WGTACW 2 cut(s) 549, 717
TfiI GAWTC 2 cut(s) 421, 871
Tru1I TTAA 2 cut(s) 357, 883
Tru9I TTAA 2 cut(s) 357, 883
TscAI CASTG 3 cut(s) 451, 797, 871
TseFI GTSAC 3 cut(s) 511, 563, 620
TseI GCWGC 2 cut(s) 335, 672
Tsp45I GTSAC 3 cut(s) 511, 563, 620
TspDTI ATGAA 1 cut(s) 686
TspGWI ACGGA 2 cut(s) 375, 573
TspRI CASTG 3 cut(s) 451, 797, 871
VpaK11BI GGWCC 2 cut(s) 256, 411
XapI RAATTY 1 cut(s) 476
XceI RCATGY 1 cut(s) 718
XspI CTAG 3 cut(s) 197, 243, 285
ZraI GACGTC 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.