Rroxscaffold_2G00146250

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010874
Physical Location & Seq
Forward (+)
84196417 .. 84197058
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146250.1

Sequence Viewer

Length: 522 bp
ATGGACGCGAGATTCTTGCAAAGTATTGTGTTTGTAGTCACAGTTCGAATCGCTCCGACCCTATTCCTGTTAACTCCGGTAAAAAAATCCGATACAACTGGTCTTCATGAACTTGAGGAAGAAGACAGTGAGGAGAATTATACCTTTGTGACTTGCCATGGAAGAAACAAGTCCATCACCAAGGTCTACTATGATGGAGTTGTTCGTACCGAGCACCATGTTAGTTTTGAACCCTGTCATGTTAACCATAACCATAAACAAAAACAAGCTCCTGTGTATCCCACGTCCGATTTTCTCAGCTCATGCCATTTGTGCAGCCAAAAGCTTCATGGCAAAGATATTTACATGTACAGGGGGGGAGAGAAGGCATTTTGTAGCACAGAGTGTAGAGCAACACAGATCATGAACGATGAGCGCAAAGAACAGTGCAGATCAGAAGCTTCAAGAAGATCTGCAGATGTTTCGAGCTCGCCTTATTCGAGAGATCAGATCTTCTTCTCTACTGGTATTCTTGCGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

173

Amino Acids

19.77

Weight (kDa)

6.74

Isoelectric Point (pI)

65.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 91 - 140 5.1e-21 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19200 AT1G19200 AT1G74940 AT5G20700
fragaria_vesca FvH4_1g09090
malus_domestica MD02G1096300.v1.1 MD15G1220800.v1.1
prunus_persica Prupe.7G194600_v2.0.a1
pyrus_communis pycom02g07610 pycom15g19560
rosa_chinensis RchiOBHm_Chr2g0095501
rosa_laevigata RLG00000016573
rosa_multiflora Rmu_sc0006076.1_g000006
rosa_roxburghii Rroxscaffold_2G00146250
rosa_rugosa Rorug02G0051700
rosa_samantha Rh2AG099700 Rh2BG101000 Rh2CG103200 Rh2DG100600
rosa_wichuraiana Rw2G007560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 384
AfaI GTAC 2 cut(s) 208, 350
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 230, 444
AjiI CACGTC 1 cut(s) 285
AluBI AGCT 5 cut(s) 269, 300, 325, 440, 468
AluI AGCT 5 cut(s) 269, 300, 325, 440, 468
Alw21I GWGCWC 2 cut(s) 216, 470
ApeKI GCWGC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 169
AsuII TTCGAA 1 cut(s) 46
BanII GRGCYC 1 cut(s) 470
BbsI GAAGAC 2 cut(s) 95, 129
Bbv12I GWGCWC 2 cut(s) 216, 470
BbvI GCAGC 1 cut(s) 327
BccI CCATC 2 cut(s) 182, 188
BcgI CGANNNNNNTGC 3 cut(s) 32, 444, 478
BciVI GTATCC 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 288
BglII AGATCT 2 cut(s) 449, 489
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 95, 129
Bpu14I TTCGAA 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 157, 180
BsaWI WCCGGW 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 103, 508
BseDI CCNNGG 2 cut(s) 157, 180
BseMII CTCAG 1 cut(s) 310
BseNI ACTGG 2 cut(s) 103, 508
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 327
BsgI GTGCAG 2 cut(s) 334, 448
Bsh1236I CGCG 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 216, 470
BsiSI CCGG 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 216, 470
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 5 cut(s) 399, 431, 449, 484, 489
Bsp19I CCATGG 1 cut(s) 157
BspCNI CTCAG 1 cut(s) 309
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 2 cut(s) 106, 402
BspMAI CTGCAG 1 cut(s) 457
BspT104I TTCGAA 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 2 cut(s) 103, 508
BssECI CCNNGG 2 cut(s) 157, 180
BssMI GATC 5 cut(s) 399, 431, 449, 484, 489
BssT1I CCWWGG 2 cut(s) 157, 180
Bst4CI ACNGT 3 cut(s) 43, 128, 426
BstAUI TGTACA 1 cut(s) 348
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 470
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 5 cut(s) 402, 434, 452, 487, 492
BstMBI GATC 5 cut(s) 399, 431, 449, 484, 489
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 327
BstV2I GAAGAC 2 cut(s) 95, 129
BstX2I RGATCY 2 cut(s) 449, 489
BstYI RGATCY 2 cut(s) 449, 489
BsuI GTATCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 157
BtrI CACGTC 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 133, 431
Cac8I GCNNGC 1 cut(s) 470
CciI TCATGA 2 cut(s) 106, 402
CfoI GCGC 1 cut(s) 417
CseI GACGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 207, 349
CviAII CATG 8 cut(s) 107, 158, 218, 239, 303, 329, 346, 403
CviJI RGCY 6 cut(s) 269, 300, 318, 325, 440, 468
CviKI_1 RGCY 6 cut(s) 269, 300, 318, 325, 440, 468
CviQI GTAC 2 cut(s) 207, 349
DdeI CTNAG 1 cut(s) 296
DpnI GATC 5 cut(s) 401, 433, 451, 486, 491
DpnII GATC 5 cut(s) 399, 431, 449, 484, 489
DraIII CACNNNGTG 1 cut(s) 384
Ecl136II GAGCTC 1 cut(s) 468
Eco130I CCWWGG 2 cut(s) 157, 180
Eco24I GRGCYC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 157, 180
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 2 cut(s) 157, 180
FaeI CATG 8 cut(s) 110, 161, 221, 242, 306, 332, 349, 406
FatI CATG 8 cut(s) 106, 157, 217, 238, 302, 328, 345, 402
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 316
GlaI GCGC 1 cut(s) 416
GluI GCNGC 1 cut(s) 316
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 417
Hin1II CATG 8 cut(s) 110, 161, 221, 242, 306, 332, 349, 406
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 2 cut(s) 72, 244
HindII GTYRAC 2 cut(s) 72, 244
HindIII AAGCTT 2 cut(s) 323, 438
HinfI GANTC 2 cut(s) 12, 48
HpaI GTTAAC 2 cut(s) 72, 244
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 3 cut(s) 72, 187, 244
Hpy188I TCNGA 5 cut(s) 57, 91, 289, 436, 489
Hpy188III TCNNGA 4 cut(s) 107, 403, 444, 480
Hpy8I GTNNAC 3 cut(s) 72, 187, 244
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 3 cut(s) 43, 128, 426
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 4 cut(s) 19, 315, 429, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 8 cut(s) 110, 161, 221, 242, 306, 332, 349, 406
HspAI GCGC 1 cut(s) 415
KspAI GTTAAC 2 cut(s) 72, 244
Kzo9I GATC 5 cut(s) 399, 431, 449, 484, 489
LmnI GCTCC 2 cut(s) 58, 274
LpnPI CCDG 7 cut(s) 80, 84, 90, 247, 285, 337, 489
Lsp1109I GCAGC 1 cut(s) 327
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 2 cut(s) 37, 148
MalI GATC 5 cut(s) 401, 433, 451, 486, 491
MboI GATC 5 cut(s) 399, 431, 449, 484, 489
MboII GAAGA 7 cut(s) 95, 131, 134, 174, 459, 484, 487
MflI RGATCY 2 cut(s) 449, 489
MhlI GDGCHC 2 cut(s) 216, 470
MluCI AATT 1 cut(s) 136
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 2 cut(s) 109, 124
MseI TTAA 2 cut(s) 71, 243
MspI CCGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 312
NcoI CCATGG 1 cut(s) 157
NdeII GATC 5 cut(s) 399, 431, 449, 484, 489
NlaIII CATG 8 cut(s) 110, 161, 221, 242, 306, 332, 349, 406
NmuCI GTSAC 2 cut(s) 37, 148
NspI RCATGY 1 cut(s) 349
NspV TTCGAA 1 cut(s) 46
PagI TCATGA 2 cut(s) 106, 402
PciI ACATGT 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PfeI GAWTC 2 cut(s) 12, 48
PkrI GCNGC 1 cut(s) 317
PscI ACATGT 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 470
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 449, 489
RsaI GTAC 2 cut(s) 208, 350
RsaNI GTAC 2 cut(s) 207, 349
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 2 cut(s) 71, 243
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 5 cut(s) 399, 431, 449, 484, 489
SduI GDGCHC 2 cut(s) 216, 470
SetI ASST 8 cut(s) 146, 186, 271, 287, 302, 327, 442, 470
SfcI CTRYAG 1 cut(s) 453
SfuI TTCGAA 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 1 cut(s) 136
SstI GAGCTC 1 cut(s) 470
StyI CCWWGG 2 cut(s) 157, 180
TaaI ACNGT 3 cut(s) 43, 128, 426
TaiI ACGT 1 cut(s) 287
TaqI TCGA 3 cut(s) 46, 464, 479
TasI AATT 1 cut(s) 136
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 2 cut(s) 12, 48
Tru1I TTAA 2 cut(s) 71, 243
Tru9I TTAA 2 cut(s) 71, 243
TscAI CASTG 2 cut(s) 133, 431
TseFI GTSAC 2 cut(s) 37, 148
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 2 cut(s) 37, 148
TspDTI ATGAA 4 cut(s) 95, 123, 317, 419
TspRI CASTG 2 cut(s) 133, 431
XceI RCATGY 1 cut(s) 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.