AT5G20920

eukaryotic translation initiation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007022
Physical Location & Seq
Reverse (-)
7094649 .. 7097145
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20920.3

Sequence Viewer

Length: 807 bp
ATGGCTGATGAAATCAATGAGATAAGGGAAGAGCAAGAACAGCTCGCACCCTTTGATCCTTCCAAGAAGAAAAAGAAGAAGAAGGTTGTGATTCAGGAACCTGTCGAGGACTTAGCAGAGTCTTCACAGACTGAGAAATCTGATTCATTGCCTGTTAATGATGGTCTTGAGAGTTCATTTACTGGAATGAAGAAAAAGAAGAAGAAGCCAACTGAATCAAGCTTATTGAACAACGAAAGTGTTGATGCTGGGGAAGATTTGGATGAGATTGCTAATGACGAGCAAGAGGGGGAAGAAGGAATAGTTCTACAGCAACGTTACCCCTGGGAGGGAAGTGAGAGGGATTACATATATGACGAGCTTCTTGGTAGAGTCTTTAACATTCTCCGTGAAAATAATCCGGAGCTTGCTGGAGATAGGCGTCGTACAGTTATGAGGCCTCCTCAAGTTCTTCGTGAGGGGACAAAGAAGACTGTCTTTGTCAACTTCATGGACCTTTGCAAGACGATGCATCGTCAACCAGATCATGTTATGCAATACTTGCTTGCTGAGTTGGGTACTAGTGGTTCGCTTGATGGGCAGCAAAGGTTGGTTGTTAAGGGAAGGTTTGCACCTAAGAATTTTGAAGGAATTTTGCGGCGATATATCACTGACTACGTCATTTGCCTTGGTTGCAAGAGCCCAGACACCATTCTCTCCAAGGAGAACCGTCTCTTCTTTCTGAGATGTGAAAAGTGTGGATCTCAACGATCTGTGGCTCCGATCAAAACAGGGTTTGTTGCTCGTGTTAGTCGCAGGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

268

Amino Acids

30.66

Weight (kDa)

6.79

Isoelectric Point (pI)

57.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 139 - 249 2.4e-40 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 1 cut(s) 637
AclI AACGTT 1 cut(s) 316
AclWI GGATC 2 cut(s) 50, 748
AcsI RAATTY 2 cut(s) 619, 630
AcyI GRCGYC 1 cut(s) 421
AfaI GTAC 2 cut(s) 427, 559
AfiI CCNNNNNNNGG 2 cut(s) 328, 329
AgsI TTSAA 2 cut(s) 229, 626
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 4 cut(s) 43, 222, 361, 406
AluI AGCT 4 cut(s) 43, 222, 361, 406
Alw26I GTCTC 1 cut(s) 716
AlwI GGATC 2 cut(s) 50, 748
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 580
ApoI RAATTY 2 cut(s) 619, 630
AspS9I GGNCC 1 cut(s) 493
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 683
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 2 cut(s) 114, 476
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 155, 569
BciT130I CCWGG 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 716
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 2 cut(s) 581, 638
BlsI GCNGC 2 cut(s) 582, 639
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 2 cut(s) 99, 759
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 3 cut(s) 235, 498, 520
BpiI GAAGAC 2 cut(s) 114, 476
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 2 cut(s) 188, 429
BsaHI GRCGYC 1 cut(s) 421
BsaJI CCNNGG 4 cut(s) 323, 324, 667, 699
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 329
Bse1I ACTGG 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 146
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 4 cut(s) 323, 324, 667, 699
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 328, 329
BseMI GCAATG 1 cut(s) 146
BseMII CTCAG 3 cut(s) 123, 540, 713
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 432
BseXI GCAGC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 248
BshFI GGCC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 2 cut(s) 328, 329
BsmAI GTCTC 1 cut(s) 716
BsmBI CGTCTC 1 cut(s) 716
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 683
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 5 cut(s) 55, 523, 740, 749, 762
BspACI CCGC 1 cut(s) 637
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 3 cut(s) 124, 541, 714
BspEI TCCGGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 99, 759
BspPI GGATC 2 cut(s) 50, 748
BspQI GCTCTTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 146
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 4 cut(s) 323, 324, 667, 699
BssMI GATC 5 cut(s) 55, 523, 740, 749, 762
BssNI GRCGYC 1 cut(s) 421
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 667, 699
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 3 cut(s) 430, 475, 710
Bst6I CTCTTC 2 cut(s) 24, 719
BstACI GRCGYC 1 cut(s) 421
BstAPI GCANNNNNTGC 1 cut(s) 541
BstC8I GCNNGC 3 cut(s) 45, 408, 546
BstDEI CTNAG 5 cut(s) 112, 132, 549, 615, 722
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 5 cut(s) 58, 526, 743, 752, 765
BstMAI GTCTC 1 cut(s) 716
BstMBI GATC 5 cut(s) 55, 523, 740, 749, 762
BstMWI GCNNNNNNNGC 4 cut(s) 40, 541, 577, 672
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 592
BstV2I GAAGAC 2 cut(s) 114, 476
BstX2I RGATCY 1 cut(s) 740
BstYI RGATCY 1 cut(s) 740
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 3 cut(s) 45, 408, 546
Cfr13I GGNCC 1 cut(s) 493
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 426, 558
CviAII CATG 2 cut(s) 490, 527
CviJI RGCY 9 cut(s) 5, 43, 208, 222, 361, 406, 439, 681, 758
CviKI_1 RGCY 9 cut(s) 5, 43, 208, 222, 361, 406, 439, 681, 758
CviQI GTAC 2 cut(s) 426, 558
DdeI CTNAG 5 cut(s) 112, 132, 549, 615, 722
DpnI GATC 5 cut(s) 57, 525, 742, 751, 764
DpnII GATC 5 cut(s) 55, 523, 740, 749, 762
Eam1104I CTCTTC 2 cut(s) 24, 719
EarI CTCTTC 2 cut(s) 24, 719
Eco130I CCWWGG 2 cut(s) 667, 699
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 683
Eco47I GGWCC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 667, 699
EcoT22I ATGCAT 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 683
ErhI CCWWGG 2 cut(s) 667, 699
Esp3I CGTCTC 1 cut(s) 716
FaeI CATG 2 cut(s) 493, 530
FaiI YATR 8 cut(s) 350, 352, 354, 434, 491, 528, 533, 645
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 1 cut(s) 475
FatI CATG 2 cut(s) 489, 526
Fnu4HI GCNGC 2 cut(s) 581, 638
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 683
Fsp4HI GCNGC 2 cut(s) 581, 638
FspBI CTAG 1 cut(s) 561
GluI GCNGC 2 cut(s) 581, 638
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 421
Hin1II CATG 2 cut(s) 493, 530
HincII GTYRAC 2 cut(s) 484, 518
HindII GTYRAC 2 cut(s) 484, 518
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 5 cut(s) 91, 119, 143, 215, 372
HpaII CCGG 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 484, 518
Hpy188I TCNGA 3 cut(s) 142, 723, 762
Hpy188III TCNNGA 4 cut(s) 95, 167, 401, 455
Hpy8I GTNNAC 2 cut(s) 484, 518
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 5 cut(s) 69, 76, 290, 597, 620
HpyCH4III ACNGT 3 cut(s) 430, 475, 710
HpyCH4IV ACGT 2 cut(s) 316, 657
HpyCH4V TGCA 5 cut(s) 501, 511, 535, 611, 675
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 541, 577, 672
HpyF3I CTNAG 5 cut(s) 112, 132, 549, 615, 722
HpySE526I ACGT 2 cut(s) 316, 657
Hsp92I GRCGYC 1 cut(s) 421
Hsp92II CATG 2 cut(s) 493, 530
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 55, 523, 740, 749, 762
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 2 cut(s) 403, 763
Lsp1109I GCAGC 1 cut(s) 592
LweI GCATC 3 cut(s) 235, 498, 520
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 316, 657
MaeIII GTNAC 1 cut(s) 317
MalI GATC 5 cut(s) 57, 525, 742, 751, 764
MboI GATC 5 cut(s) 55, 523, 740, 749, 762
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 1 cut(s) 683
MluCI AATT 2 cut(s) 619, 630
MlyI GAGTC 2 cut(s) 128, 381
MnlI CCTC 8 cut(s) 100, 280, 322, 333, 429, 450, 451, 453
Mph1103I ATGCAT 1 cut(s) 513
MroI TCCGGA 1 cut(s) 400
MseI TTAA 3 cut(s) 156, 378, 597
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 40, 541, 577, 672
NdeII GATC 5 cut(s) 55, 523, 740, 749, 762
NlaIII CATG 2 cut(s) 493, 530
NlaIV GGNNCC 2 cut(s) 99, 759
NsiI ATGCAT 1 cut(s) 513
PasI CCCWGGG 1 cut(s) 324
PceI AGGCCT 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 24
PfeI GAWTC 3 cut(s) 91, 143, 215
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 2 cut(s) 582, 639
PleI GAGTC 2 cut(s) 127, 380
PpsI GAGTC 2 cut(s) 127, 380
Psp1406I AACGTT 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 2 cut(s) 99, 759
PspPI GGNCC 1 cut(s) 493
PsrI GAACNNNNNNTAC 2 cut(s) 550, 582
PsuI RGATCY 1 cut(s) 740
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 2 cut(s) 427, 559
RsaNI GTAC 2 cut(s) 426, 558
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 3 cut(s) 156, 378, 597
SatI GCNGC 2 cut(s) 581, 638
Sau3AI GATC 5 cut(s) 55, 523, 740, 749, 762
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 2 cut(s) 128, 381
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 683
SfaNI GCATC 3 cut(s) 235, 498, 520
SfcI CTRYAG 1 cut(s) 308
SinI GGWCC 1 cut(s) 493
SmlI CTYRAG 2 cut(s) 167, 444
SmoI CTYRAG 2 cut(s) 167, 444
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 2 cut(s) 619, 630
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 1 cut(s) 637
SspMI CTAG 1 cut(s) 561
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 2 cut(s) 667, 699
TaaI ACNGT 3 cut(s) 430, 475, 710
TaiI ACGT 2 cut(s) 319, 660
TaqI TCGA 1 cut(s) 105
TasI AATT 2 cut(s) 619, 630
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 3 cut(s) 91, 143, 215
Tru1I TTAA 3 cut(s) 156, 378, 597
Tru9I TTAA 3 cut(s) 156, 378, 597
TscAI CASTG 1 cut(s) 655
TseI GCWGC 1 cut(s) 580
TspDTI ATGAA 5 cut(s) 24, 135, 165, 203, 478
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 655
Tth111I GACNNNGTC 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 2 cut(s) 619, 630
XspI CTAG 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.