Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0007022

Physical Location & Seq

Genomic Coordinates Reverse (-)

80047527 .. 80052382

Gene Structure 4856 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG514400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1023 bp

ATGGCGAAGTTGAAGTCGCTGGACGTGAAGGAGGCAAAGTCGCTGCTCCAGTGCTGCCGCGACCACGGCGTCAACTTCTTCGACAACGCGGAGGTCTACGCGAGCGGCCGCGCCGAGGAGATCATGGGCCAGGCCATACGCGATCTGGGCTGGAAGCGCTCCGACATCGTCGTCTCCACCAAGATCAGAGATTTAGATCAGAAGACTGAGAAGAGAAGAGAAGAGAACAACCATGCTGAAGAGAACAAGAATGCTGTGAAGGAGGAGGTTCCAGATATTGTCCCCTTTGATCGTACTAAAAAGAAGAAAAAGAAGAAGGTGGTAATTCGGGATTCTTCAAGCGATTCTGTGGACAAGTTGGCTGACAAAACAGAAAACCTAGCAGTTTCTGATGGACAGGAAAGTGCATTTTCTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAATCCAGTGTCTTGAATGAAGAGGGTGGTGATACAAGGGAAGATTTGGATGACGGGACTGGGGATGATGAAGAGGGAGAAGGAATTGTTTTAGGGGCCTCGCATCCATGGGAAGGGAGTGATCGTGACTACCGCTATGAGGAGCTTCTTGGTAGGGTGTTCAATATCCTTCGGGAGAATAATCCTGAGCTGGCTGGCGACAGGCGTAGGACAACTATGAGGCCTCCACAAGTACTTCGTGAGGGCACAAAGAAAACTGTCTTTGTTAATTTCATGGACTTATGTAAGACGATGCATAGGCAGCCGGATCATGTTATGGCCTTTTTACTGGCTGAACTGGGGACAAGTGGATCCCTTGACGGTCAGCAGAGGTTAGTTGTAAAGGGAAGATTTGCACCCAAGAATTTTGAAGGAATACTACGGCGCTATGTCAACGAATATGTCATTTGCATTGGGTGCAAGAGCCCTGACACTATACTTTCCAAGGAGAATCGCCTCTTCTTTCTCAGATGCGAGAAGGTAACTGTCATAAACCAGGCTATTAGTTTGTTGATTTTACTAGTTGGTCAATTTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

eIF-5_eIF-2B

Protein Analysis

340

Amino Acids

38.51

Weight (kDa)

7.58

Isoelectric Point (pI)

40.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aldo_ket_red	PF00248	7 - 81	3.4e-11	Aldo/keto reductase family
eIF-5_eIF-2B	PF01873	215 - 321	4.6e-37	Domain found in IF2B/IF5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20920 AT5G20920 AT5G20920
rosa_chinensis	RchiOBHm_Chr2g0109961 RchiOBHm_Chr5g0073521
rosa_laevigata	RLG00000017774 RLG00000036390
rosa_multiflora	Rmu_co8275511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007750
rosa_rugosa	Rorug02G0164300 Rorug02G0164400 Rorug02G0164500 Rorug02G0164600 Rorug05G0424200 Rorug05G0424300
rosa_samantha	Rh2AG216200 Rh2BG226300 Rh2CG218400 Rh2DG221800 Rh5AG481500 Rh5BG502200 Rh5CG525400 Rh5DG514400
rosa_wichuraiana	Rw2G016700 Rw5G044730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	68, 170
AccBSI	CCGCTC	1 cut(s)	105
AccI	GTMKAC	1 cut(s)	96
AccII	CGCG	5 cut(s)	60, 89, 101, 111, 141
AciI	CCGC	5 cut(s)	58, 89, 105, 109, 577
AclWI	GGATC	3 cut(s)	759, 789, 802
AcoI	YGGCCR	1 cut(s)	106
AcsI	RAATTY	1 cut(s)	847
AcuI	CTGAAG	1 cut(s)	258
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	2 cut(s)	295, 678
AfeI	AGCGCT	1 cut(s)	158
AfiI	CCNNNNNNNGG	3 cut(s)	115, 557, 583
AgsI	TTSAA	7 cut(s)	13, 339, 421, 446, 460, 607, 854
AhlI	ACTAGT	1 cut(s)	1003
AjiI	CACGTC	1 cut(s)	25
AjnI	CCWGG	2 cut(s)	129, 978
AluBI	AGCT	2 cut(s)	589, 634
AluI	AGCT	2 cut(s)	589, 634
Alw26I	GTCTC	1 cut(s)	178
AlwI	GGATC	3 cut(s)	759, 789, 802
AlwNI	CAGNNNCTG	1 cut(s)	389
Aor51HI	AGCGCT	1 cut(s)	158
AoxI	GGCC	6 cut(s)	106, 127, 132, 540, 665, 762
ApeKI	GCWGC	3 cut(s)	43, 54, 745
ApoI	RAATTY	1 cut(s)	847
ArsI	GACNNNNNNTTYG	1 cut(s)	31
AspLEI	GCGC	3 cut(s)	113, 159, 870
AspS9I	GGNCC	2 cut(s)	127, 540
AsuHPI	GGTGA	1 cut(s)	485
BaeGI	GKGCMC	1 cut(s)	692
BamHI	GGATCC	1 cut(s)	794
BanII	GRGCYC	1 cut(s)	911
BarI	GAAGNNNNNNTAC	2 cut(s)	846, 878
BbsI	GAAGAC	1 cut(s)	209
BbvI	GCAGC	3 cut(s)	30, 41, 757
BccI	CCATC	1 cut(s)	386
BceAI	ACGGC	2 cut(s)	82, 881
BciT130I	CCWGG	2 cut(s)	131, 980
BcoDI	GTCTC	1 cut(s)	178
BcuI	ACTAGT	1 cut(s)	1003
BfaI	CTAG	2 cut(s)	380, 1004
BfoI	RGCGCY	2 cut(s)	160, 871
BisI	GCNGC	6 cut(s)	44, 55, 58, 106, 109, 746
BlsI	GCNGC	6 cut(s)	45, 56, 59, 107, 110, 747
BmcAI	AGTACT	1 cut(s)	678
Bme1390I	CCNGG	2 cut(s)	131, 980
BmgBI	CACGTC	1 cut(s)	25
BmgT120I	GGNCC	2 cut(s)	127, 540
BmiI	GGNNCC	3 cut(s)	270, 541, 796
BmrFI	CCNGG	2 cut(s)	131, 980
BmrI	ACTGGG	2 cut(s)	513, 791
BmsI	GCATC	3 cut(s)	556, 726, 944
BmuI	ACTGGG	2 cut(s)	513, 791
BpiI	GAAGAC	1 cut(s)	209
BpmI	CTGGAG	1 cut(s)	32
Bpu10I	CCTNAGC	1 cut(s)	630
BsaBI	GATNNNNATC	1 cut(s)	195
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	4 cut(s)	64, 114, 551, 927
BsaXI	ACNNNNNCTCC	4 cut(s)	23, 53, 553, 583
Bsc4I	CCNNNNNNNGG	3 cut(s)	115, 557, 583
Bse1I	ACTGG	6 cut(s)	49, 440, 450, 508, 777, 786
Bse8I	GATNNNNATC	1 cut(s)	195
BseBI	CCWGG	2 cut(s)	131, 980
BseDI	CCNNGG	4 cut(s)	64, 114, 551, 927
BseGI	GGATG	3 cut(s)	499, 514, 547
BseJI	GATNNNNATC	1 cut(s)	195
BseLI	CCNNNNNNNGG	3 cut(s)	115, 557, 583
BseMII	CTCAG	3 cut(s)	198, 621, 964
BseNI	ACTGG	6 cut(s)	49, 440, 450, 508, 777, 786
BseRI	GAGGAG	3 cut(s)	131, 278, 599
BseSI	GKGCMC	1 cut(s)	692
BseX3I	CGGCCG	1 cut(s)	106
BseXI	GCAGC	3 cut(s)	30, 41, 757
Bsh1236I	CGCG	5 cut(s)	60, 89, 101, 111, 141
Bsh1285I	CGRYCG	1 cut(s)	109
BshFI	GGCC	6 cut(s)	108, 129, 134, 542, 667, 764
BsiEI	CGRYCG	1 cut(s)	109
BsiSI	CCGG	1 cut(s)	749
BslFI	GGGAC	3 cut(s)	266, 514, 799
BslI	CCNNNNNNNGG	3 cut(s)	115, 557, 583
BsmAI	GTCTC	1 cut(s)	178
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	3 cut(s)	266, 514, 799
BsmI	GAATGC	1 cut(s)	256
BsnI	GGCC	6 cut(s)	108, 129, 134, 542, 667, 764
Bsp1286I	GDGCHC	2 cut(s)	692, 911
Bsp143I	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
Bsp19I	CCATGG	1 cut(s)	551
BspACI	CCGC	5 cut(s)	58, 89, 105, 109, 577
BspANI	GGCC	6 cut(s)	108, 129, 134, 542, 667, 764
BspCNI	CTCAG	3 cut(s)	199, 622, 963
BspFNI	CGCG	5 cut(s)	60, 89, 101, 111, 141
BspLI	GGNNCC	3 cut(s)	270, 541, 796
BspPI	GGATC	3 cut(s)	759, 789, 802
BsrBI	CCGCTC	1 cut(s)	105
BsrI	ACTGG	6 cut(s)	49, 440, 450, 508, 777, 786
BssECI	CCNNGG	4 cut(s)	64, 114, 551, 927
BssMI	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	2 cut(s)	551, 927
Bst2UI	CCWGG	2 cut(s)	131, 980
Bst4CI	ACNGT	3 cut(s)	703, 806, 970
Bst6I	CTCTTC	7 cut(s)	206, 211, 216, 234, 459, 510, 947
BstACI	GRCGYC	1 cut(s)	69
BstAPI	GCANNNNNTGC	1 cut(s)	900
BstC8I	GCNNGC	3 cut(s)	103, 636, 640
BstDEI	CTNAG	3 cut(s)	207, 630, 950
BstDSI	CCRYGG	2 cut(s)	64, 551
BstF5I	GGATG	3 cut(s)	499, 514, 547
BstFNI	CGCG	5 cut(s)	60, 89, 101, 111, 141
BstH2I	RGCGCY	2 cut(s)	160, 871
BstHHI	GCGC	3 cut(s)	113, 159, 870
BstKTI	GATC	8 cut(s)	123, 145, 186, 199, 292, 568, 754, 797
BstMAI	GTCTC	1 cut(s)	178
BstMBI	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
BstMCI	CGRYCG	1 cut(s)	109
BstMWI	GCNNNNNNNGC	5 cut(s)	66, 147, 156, 745, 900
BstNI	CCWGG	2 cut(s)	131, 980
BstSCI	CCNGG	2 cut(s)	129, 978
BstSLI	GKGCMC	1 cut(s)	692
BstUI	CGCG	5 cut(s)	60, 89, 101, 111, 141
BstV1I	GCAGC	3 cut(s)	30, 41, 757
BstV2I	GAAGAC	1 cut(s)	209
BstX2I	RGATCY	1 cut(s)	794
BstYI	RGATCY	1 cut(s)	794
BstZI	CGGCCG	1 cut(s)	106
BsuRI	GGCC	6 cut(s)	108, 129, 134, 542, 667, 764
BtgI	CCRYGG	2 cut(s)	64, 551
BtrI	CACGTC	1 cut(s)	25
BtsCI	GGATG	3 cut(s)	499, 514, 547
BtsIMutI	CAGTG	2 cut(s)	56, 457
Cac8I	GCNNGC	3 cut(s)	103, 636, 640
CaiI	CAGNNNCTG	1 cut(s)	389
CciNI	GCGGCCGC	1 cut(s)	106
CfoI	GCGC	3 cut(s)	113, 159, 870
Cfr13I	GGNCC	2 cut(s)	127, 540
CseI	GACGC	1 cut(s)	58
Csp6I	GTAC	2 cut(s)	294, 677
CviAII	CATG	5 cut(s)	124, 233, 552, 718, 755
CviQI	GTAC	2 cut(s)	294, 677
DdeI	CTNAG	3 cut(s)	207, 630, 950
DpnI	GATC	8 cut(s)	122, 144, 185, 198, 291, 567, 753, 796
DpnII	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
DrdI	GACNNNNNNGTC	2 cut(s)	68, 170
DseDI	GACNNNNNNGTC	2 cut(s)	68, 170
EaeI	YGGCCR	1 cut(s)	106
EagI	CGGCCG	1 cut(s)	106
Eam1104I	CTCTTC	7 cut(s)	206, 211, 216, 234, 459, 510, 947
EarI	CTCTTC	7 cut(s)	206, 211, 216, 234, 459, 510, 947
EclXI	CGGCCG	1 cut(s)	106
Eco130I	CCWWGG	2 cut(s)	551, 927
Eco147I	AGGCCT	1 cut(s)	667
Eco24I	GRGCYC	1 cut(s)	911
Eco47III	AGCGCT	1 cut(s)	158
Eco52I	CGGCCG	1 cut(s)	106
Eco57I	CTGAAG	1 cut(s)	258
EcoO109I	RGGNCCY	1 cut(s)	540
EcoRII	CCWGG	2 cut(s)	129, 978
EcoT14I	CCWWGG	2 cut(s)	551, 927
EcoT22I	ATGCAT	1 cut(s)	741
EcoT38I	GRGCYC	1 cut(s)	911
ErhI	CCWWGG	2 cut(s)	551, 927
Esp3I	CGTCTC	1 cut(s)	178
FaeI	CATG	5 cut(s)	127, 236, 555, 721, 758
FaqI	GGGAC	3 cut(s)	266, 514, 799
FatI	CATG	5 cut(s)	123, 232, 551, 717, 754
FblI	GTMKAC	1 cut(s)	96
Fnu4HI	GCNGC	6 cut(s)	44, 55, 58, 106, 109, 746
FokI	GGATG	3 cut(s)	506, 521, 534
FriOI	GRGCYC	1 cut(s)	911
Fsp4HI	GCNGC	6 cut(s)	44, 55, 58, 106, 109, 746
FspBI	CTAG	2 cut(s)	380, 1004
GlaI	GCGC	3 cut(s)	112, 158, 869
GluI	GCNGC	6 cut(s)	44, 55, 58, 106, 109, 746
GsuI	CTGGAG	1 cut(s)	32
HaeII	RGCGCY	2 cut(s)	160, 871
HaeIII	GGCC	6 cut(s)	108, 129, 134, 542, 667, 764
HapII	CCGG	1 cut(s)	749
HgaI	GACGC	1 cut(s)	58
HhaI	GCGC	3 cut(s)	113, 159, 870
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	5 cut(s)	127, 236, 555, 721, 758
Hin6I	GCGC	3 cut(s)	111, 157, 868
HinP1I	GCGC	3 cut(s)	111, 157, 868
HincII	GTYRAC	2 cut(s)	73, 877
HindII	GTYRAC	2 cut(s)	73, 877
HinfI	GANTC	4 cut(s)	332, 344, 446, 934
HpaII	CCGG	1 cut(s)	749
HphI	GGTGA	1 cut(s)	485
Hpy166II	GTNNAC	4 cut(s)	73, 97, 352, 877
Hpy188I	TCNGA	5 cut(s)	163, 188, 201, 391, 953
Hpy188III	TCNNGA	7 cut(s)	272, 329, 457, 569, 617, 629, 683
Hpy8I	GTNNAC	4 cut(s)	73, 97, 352, 877
Hpy99I	CGWCG	1 cut(s)	173
HpyAV	CCTTC	8 cut(s)	22, 253, 310, 518, 551, 623, 848, 955
HpyCH4III	ACNGT	3 cut(s)	703, 806, 970
HpyCH4IV	ACGT	1 cut(s)	24
HpyCH4V	TGCA	5 cut(s)	407, 739, 839, 894, 903
HpyF10VI	GCNNNNNNNGC	5 cut(s)	66, 147, 156, 745, 900
HpyF3I	CTNAG	3 cut(s)	207, 630, 950
HpySE526I	ACGT	1 cut(s)	24
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	5 cut(s)	127, 236, 555, 721, 758
HspAI	GCGC	3 cut(s)	111, 157, 868
Kzo9I	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
LmnI	GCTCC	3 cut(s)	51, 164, 586
Lsp1109I	GCAGC	3 cut(s)	30, 41, 757
LweI	GCATC	3 cut(s)	556, 726, 944
MaeI	CTAG	2 cut(s)	380, 1004
MaeII	ACGT	1 cut(s)	24
MaeIII	GTNAC	2 cut(s)	569, 964
MalI	GATC	8 cut(s)	122, 144, 185, 198, 291, 567, 753, 796
MbiI	CCGCTC	1 cut(s)	105
MboI	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
MflI	RGATCY	1 cut(s)	794
MhlI	GDGCHC	2 cut(s)	692, 911
MluCI	AATT	5 cut(s)	324, 528, 712, 847, 1013
MmeI	TCCRAC	1 cut(s)	186
Mph1103I	ATGCAT	1 cut(s)	741
MseI	TTAA	2 cut(s)	711, 1017
MspI	CCGG	1 cut(s)	749
MspR9I	CCNGG	2 cut(s)	131, 980
Mva1269I	GAATGC	1 cut(s)	256
MvaI	CCWGG	2 cut(s)	131, 980
MvnI	CGCG	5 cut(s)	60, 89, 101, 111, 141
MwoI	GCNNNNNNNGC	5 cut(s)	66, 147, 156, 745, 900
NcoI	CCATGG	1 cut(s)	551
NdeII	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
NlaIII	CATG	5 cut(s)	127, 236, 555, 721, 758
NlaIV	GGNNCC	3 cut(s)	270, 541, 796
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	1 cut(s)	569
NotI	GCGGCCGC	1 cut(s)	106
NsiI	ATGCAT	1 cut(s)	741
PceI	AGGCCT	1 cut(s)	667
PctI	GAATGC	1 cut(s)	256
PfeI	GAWTC	4 cut(s)	332, 344, 446, 934
PflFI	GACNNNGTC	1 cut(s)	167
PkrI	GCNGC	6 cut(s)	45, 56, 59, 107, 110, 747
Psp6I	CCWGG	2 cut(s)	129, 978
PspGI	CCWGG	2 cut(s)	129, 978
PspN4I	GGNNCC	3 cut(s)	270, 541, 796
PspPI	GGNCC	2 cut(s)	127, 540
PstNI	CAGNNNCTG	1 cut(s)	389
PsuI	RGATCY	1 cut(s)	794
PsyI	GACNNNGTC	1 cut(s)	167
RsaI	GTAC	2 cut(s)	295, 678
RsaNI	GTAC	2 cut(s)	294, 677
SaqAI	TTAA	2 cut(s)	711, 1017
SatI	GCNGC	6 cut(s)	44, 55, 58, 106, 109, 746
Sau3AI	GATC	8 cut(s)	120, 142, 183, 196, 289, 565, 751, 794
Sau96I	GGNCC	2 cut(s)	127, 540
ScaI	AGTACT	1 cut(s)	678
ScrFI	CCNGG	2 cut(s)	131, 980
SduI	GDGCHC	2 cut(s)	692, 911
SetI	ASST	9 cut(s)	27, 96, 270, 321, 381, 591, 636, 818, 966
SfaNI	GCATC	3 cut(s)	556, 726, 944
SpeI	ACTAGT	1 cut(s)	1003
Sse9I	AATT	5 cut(s)	324, 528, 712, 847, 1013
SseBI	AGGCCT	1 cut(s)	667
SsiI	CCGC	5 cut(s)	58, 89, 105, 109, 577
SspMI	CTAG	2 cut(s)	380, 1004
StuI	AGGCCT	1 cut(s)	667
StyD4I	CCNGG	2 cut(s)	129, 978
StyI	CCWWGG	2 cut(s)	551, 927
TaaI	ACNGT	3 cut(s)	703, 806, 970
TaiI	ACGT	1 cut(s)	27
TaqI	TCGA	1 cut(s)	81
TasI	AATT	5 cut(s)	324, 528, 712, 847, 1013
TatI	WGTACW	1 cut(s)	676
TauI	GCSGC	3 cut(s)	60, 108, 111
TfiI	GAWTC	4 cut(s)	332, 344, 446, 934
Tru1I	TTAA	2 cut(s)	711, 1017
Tru9I	TTAA	2 cut(s)	711, 1017
TscAI	CASTG	2 cut(s)	56, 457
TseFI	GTSAC	1 cut(s)	569
TseI	GCWGC	3 cut(s)	43, 54, 745
Tsp45I	GTSAC	1 cut(s)	569
TspDTI	ATGAA	3 cut(s)	477, 528, 706
TspRI	CASTG	2 cut(s)	56, 457
Tth111I	GACNNNGTC	1 cut(s)	167
XapI	RAATTY	1 cut(s)	847
XcmI	CCANNNNNNNNNTGG	1 cut(s)	142
XmiI	GTMKAC	1 cut(s)	96
XspI	CTAG	2 cut(s)	380, 1004
ZrmI	AGTACT	1 cut(s)	678
Zsp2I	ATGCAT	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.