Rorug05G0424200

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007022
Physical Location & Seq
Reverse (-)
58943809 .. 58944355
547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0424200.1

Sequence Viewer

Length: 333 bp
ATGGCTCGAGGCTCATCTCAGTCCCAAGCGACGTCGTCTTCCACGACCTCACGCCCCGGCGTCATGGCTCCTCGTGGTTCAGCTGCCGCCACAGCTGGAATGCGCCGCCGTCGTCTCGGCGGAGGCGGCAACTCCAGCTCCGGAAGCATCGGCGGCGGCGGAGGCGGCTCTGGTGCCGGAAACAACATGCTCAGGTTCTACACCGACGACGCTCCTGGCTTGAAGATCTCCCCCACCGTCGTTCTCGTCATGAGCCTCTGCTTCATCGGCTTCGTCACCGCCCTCCACGTCTTCGGCAAGCTCTACCTCCACCGATCTACCGGCGGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

110

Amino Acids

10.92

Weight (kDa)

11.72

Isoelectric Point (pI)

66.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 60 - 99 1.1e-18 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 140
AcyI GRCGYC 2 cut(s) 32, 60
AgsI TTSAA 1 cut(s) 223
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 5 cut(s) 83, 95, 138, 301, 329
AluI AGCT 5 cut(s) 83, 95, 138, 301, 329
Alw26I GTCTC 1 cut(s) 119
Ama87I CYCGRG 1 cut(s) 6
Aor13HI TCCGGA 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 6
BanI GGYRCC 1 cut(s) 173
BauI CACGAG 1 cut(s) 72
BbsI GAAGAC 2 cut(s) 30, 283
BbvI GCAGC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 216
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 119
BglII AGATCT 1 cut(s) 225
BisI GCNGC 7 cut(s) 84, 87, 106, 127, 154, 157, 166
BlsI GCNGC 7 cut(s) 85, 88, 107, 128, 155, 158, 167
Bme1390I CCNGG 2 cut(s) 57, 216
BmeT110I CYCGRG 1 cut(s) 6
BmgBI CACGTC 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 69, 175
BmrFI CCNGG 2 cut(s) 57, 216
BmsI GCATC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 30, 283
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 32, 60
BsaJI CCNNGG 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse118I RCCGGY 1 cut(s) 320
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 55
BseMII CTCAG 2 cut(s) 32, 205
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 4 cut(s) 57, 141, 177, 321
BslFI GGGAC 1 cut(s) 7
BsmAI GTCTC 1 cut(s) 119
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 7
BsmI GAATGC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 6
Bsp13I TCCGGA 1 cut(s) 140
Bsp143I GATC 2 cut(s) 225, 314
BspCNI CTCAG 2 cut(s) 31, 204
BspEI TCCGGA 1 cut(s) 140
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 69, 175
BspT107I GGYRCC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 2 cut(s) 225, 314
BssNI GRCGYC 2 cut(s) 32, 60
BssSI CACGAG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 32, 60
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 18, 191
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 228, 317
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 2 cut(s) 225, 314
BstMWI GCNNNNNNNGC 8 cut(s) 92, 126, 135, 144, 153, 162, 165, 267
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 2 cut(s) 55, 214
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 2 cut(s) 30, 283
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BtrI CACGTC 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 299
CciI TCATGA 1 cut(s) 249
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 320
CseI GACGC 2 cut(s) 49, 218
CviAII CATG 3 cut(s) 64, 187, 250
DdeI CTNAG 2 cut(s) 18, 191
DpnI GATC 2 cut(s) 227, 316
DpnII GATC 2 cut(s) 225, 314
EciI GGCGGA 2 cut(s) 135, 174
Eco88I CYCGRG 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 214
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 3 cut(s) 67, 190, 253
FaiI YATR 3 cut(s) 65, 188, 251
FaqI GGGAC 1 cut(s) 7
FatI CATG 3 cut(s) 63, 186, 249
Fnu4HI GCNGC 7 cut(s) 84, 87, 106, 127, 154, 157, 166
Fsp4HI GCNGC 7 cut(s) 84, 87, 106, 127, 154, 157, 166
GlaI GCGC 1 cut(s) 104
GluI GCNGC 7 cut(s) 84, 87, 106, 127, 154, 157, 166
GsuI CTGGAG 1 cut(s) 118
HapII CCGG 4 cut(s) 57, 141, 177, 321
HgaI GACGC 2 cut(s) 49, 218
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 2 cut(s) 32, 60
Hin1II CATG 3 cut(s) 67, 190, 253
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HpaII CCGG 4 cut(s) 57, 141, 177, 321
HphI GGTGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 141, 250
Hpy99I CGWCG 6 cut(s) 34, 37, 114, 209, 212, 242
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 32, 288
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 126, 135, 144, 153, 162, 165, 267
HpyF3I CTNAG 2 cut(s) 18, 191
HpySE526I ACGT 2 cut(s) 32, 288
Hsp92I GRCGYC 2 cut(s) 32, 60
Hsp92II CATG 3 cut(s) 67, 190, 253
HspAI GCGC 1 cut(s) 103
Kpn2I TCCGGA 1 cut(s) 140
Kzo9I GATC 2 cut(s) 225, 314
LmnI GCTCC 4 cut(s) 73, 143, 217, 326
LpnPI CCDG 9 cut(s) 70, 81, 148, 154, 156, 178, 190, 201, 228
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 156
MaeII ACGT 2 cut(s) 32, 288
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 227, 316
MboI GATC 2 cut(s) 225, 314
MboII GAAGA 3 cut(s) 30, 235, 283
MflI RGATCY 1 cut(s) 225
MnlI CCTC 7 cut(s) 58, 81, 116, 155, 266, 293, 317
MroI TCCGGA 1 cut(s) 140
MseI TTAA 1 cut(s) 331
MspA1I CMGCKG 2 cut(s) 83, 95
MspI CCGG 4 cut(s) 57, 141, 177, 321
MspR9I CCNGG 2 cut(s) 57, 216
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 8 cut(s) 92, 126, 135, 144, 153, 162, 165, 267
NciI CCSGG 1 cut(s) 57
NdeII GATC 2 cut(s) 225, 314
NlaIII CATG 3 cut(s) 67, 190, 253
NlaIV GGNNCC 2 cut(s) 69, 175
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 6
PagI TCATGA 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PctI GAATGC 1 cut(s) 105
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 7 cut(s) 85, 88, 107, 128, 155, 158, 167
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 69, 175
PspXI VCTCGAGB 1 cut(s) 6
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 2 cut(s) 83, 95
SaqAI TTAA 1 cut(s) 331
SatI GCNGC 7 cut(s) 84, 87, 106, 127, 154, 157, 166
Sau3AI GATC 2 cut(s) 225, 314
ScrFI CCNGG 2 cut(s) 57, 216
SfaNI GCATC 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 6
SlaI CTCGAG 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 55, 214
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 35, 291
TaqI TCGA 1 cut(s) 7
TauI GCSGC 6 cut(s) 89, 108, 129, 156, 159, 168
Tru1I TTAA 1 cut(s) 331
Tru9I TTAA 1 cut(s) 331
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 1 cut(s) 253
Tth111I GACNNNGTC 1 cut(s) 34
XceI RCATGY 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 6
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.