Rh5CG525400

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007022
Physical Location & Seq
Reverse (-)
73565865 .. 73568613
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG525400.1

Sequence Viewer

Length: 810 bp
ATGGCTGAAGAGAACAAGAATGCTGTGAAGGAGGAGGTTCCAGATATTGTCCCCTTTGATCGTACTAAAAAGAAGAAAAAGAAGAAGGTGGTAATTCGGGATTCTTCAAGAGATTCTGTGGACAAGTTGGCTGACAAAACAGAAAACCTAGCAGTTTCTGATGGACAGGAAAGTGCATTTTCTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAATCCAGTGTCTTGAATGAAGAGGGTGGTGATACAAGGGAAGATTTGGATGACGGGACTGGGGATGATGAAGAGGGAGAAGGAATTGTTTTAGGGGCCTCGCATCCATGGGAAGGGAGTGATCGTGACTACCACTATGAGGAGCTTCTTGGTAGGGTGTTCAATATCCTTCGGGAGAATAATCCTGAGCTGGCTGGCGACAGGCGTAGGACAACTATGAGGCCTCCACAAGTACTTCGTGAGGGCACAAAGAAAACTGTCTTTGTTAATTTCATGGACTTATGTAAGACGATGCATAGGCAGCCGGATCATGTTATGGCCTTTTTACTGGCTGAACTGGGGACAAGTGGATCCCTTGACGGTCAGCAGAGGTTAGTTGTAAAGGGAAGATTTGCACCCAAGAATTTTGAAGGAATACTACGGCGCTATGTCAACGAATATGTCATTTGCATTGGGTGCAAGAGTCCTGACACTATACTTTCCAAGGAGAATCGCCTCTTTTTTCTCAGATGTGAGAAGTGTGGTTCCGGACGATCTGTTGCTCCAATTAAAGCTGGTTTTGTTGCTCGTGTTGGGCGAAGAAATGCTGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

269

Amino Acids

30.12

Weight (kDa)

8.84

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 138 - 247 5.6e-40 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 740
AclWI GGATC 3 cut(s) 528, 558, 571
AcsI RAATTY 1 cut(s) 616
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 2 cut(s) 64, 447
AfiI CCNNNNNNNGG 2 cut(s) 326, 352
AgsI TTSAA 6 cut(s) 108, 190, 215, 229, 376, 623
AluBI AGCT 3 cut(s) 358, 403, 767
AluI AGCT 3 cut(s) 358, 403, 767
AlwI GGATC 3 cut(s) 528, 558, 571
AlwNI CAGNNNCTG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 740
AoxI GGCC 3 cut(s) 309, 434, 531
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 616
AspLEI GCGC 1 cut(s) 639
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 461
BamHI GGATCC 1 cut(s) 563
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BauI CACGAG 1 cut(s) 780
BbvI GCAGC 1 cut(s) 526
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 650
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 640
BisI GCNGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 516
BmcAI AGTACT 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 4 cut(s) 39, 310, 565, 739
BmrI ACTGGG 2 cut(s) 282, 560
BmsI GCATC 2 cut(s) 325, 495
BmuI ACTGGG 2 cut(s) 282, 560
Bpu10I CCTNAGC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 320, 696
BsaWI WCCGGW 1 cut(s) 740
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 352
Bse1I ACTGG 5 cut(s) 209, 219, 277, 546, 555
BseAI TCCGGA 1 cut(s) 740
BseDI CCNNGG 2 cut(s) 320, 696
BseGI GGATG 3 cut(s) 268, 283, 316
BseLI CCNNNNNNNGG 2 cut(s) 326, 352
BseMII CTCAG 2 cut(s) 390, 733
BseNI ACTGG 5 cut(s) 209, 219, 277, 546, 555
BseRI GAGGAG 2 cut(s) 47, 368
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 526
BseYI CCCAGC 1 cut(s) 800
BshFI GGCC 3 cut(s) 311, 436, 533
BsiSI CCGG 2 cut(s) 518, 741
BslFI GGGAC 3 cut(s) 35, 283, 568
BslI CCNNNNNNNGG 2 cut(s) 326, 352
BsmFI GGGAC 3 cut(s) 35, 283, 568
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 3 cut(s) 311, 436, 533
Bsp1286I GDGCHC 1 cut(s) 461
Bsp13I TCCGGA 1 cut(s) 740
Bsp143I GATC 5 cut(s) 58, 334, 520, 563, 746
Bsp19I CCATGG 1 cut(s) 320
BspANI GGCC 3 cut(s) 311, 436, 533
BspCNI CTCAG 2 cut(s) 391, 732
BspEI TCCGGA 1 cut(s) 740
BspLI GGNNCC 4 cut(s) 39, 310, 565, 739
BspPI GGATC 3 cut(s) 528, 558, 571
BsrI ACTGG 5 cut(s) 209, 219, 277, 546, 555
BssECI CCNNGG 2 cut(s) 320, 696
BssMI GATC 5 cut(s) 58, 334, 520, 563, 746
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 320, 696
Bst2BI CACGAG 1 cut(s) 780
Bst4CI ACNGT 2 cut(s) 472, 575
Bst6I CTCTTC 3 cut(s) 3, 228, 279
BstAPI GCANNNNNTGC 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 405, 409
BstDEI CTNAG 2 cut(s) 399, 719
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 3 cut(s) 268, 283, 316
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 1 cut(s) 639
BstKTI GATC 5 cut(s) 61, 337, 523, 566, 749
BstMBI GATC 5 cut(s) 58, 334, 520, 563, 746
BstMWI GCNNNNNNNGC 2 cut(s) 514, 669
BstSLI GKGCMC 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 526
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BsuRI GGCC 3 cut(s) 311, 436, 533
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 3 cut(s) 268, 283, 316
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 405, 409
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 639
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 63, 446
CviAII CATG 4 cut(s) 321, 487, 524, 807
CviQI GTAC 2 cut(s) 63, 446
DdeI CTNAG 2 cut(s) 399, 719
DpnI GATC 5 cut(s) 60, 336, 522, 565, 748
DpnII GATC 5 cut(s) 58, 334, 520, 563, 746
Eam1104I CTCTTC 3 cut(s) 3, 228, 279
EarI CTCTTC 3 cut(s) 3, 228, 279
Eco130I CCWWGG 2 cut(s) 320, 696
Eco147I AGGCCT 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 320, 696
EcoT22I ATGCAT 1 cut(s) 510
ErhI CCWWGG 2 cut(s) 320, 696
FaeI CATG 4 cut(s) 324, 490, 527, 810
FaqI GGGAC 3 cut(s) 35, 283, 568
FatI CATG 4 cut(s) 320, 486, 523, 806
Fnu4HI GCNGC 1 cut(s) 515
FokI GGATG 3 cut(s) 275, 290, 303
Fsp4HI GCNGC 1 cut(s) 515
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 638
GluI GCNGC 1 cut(s) 515
GsaI CCCAGC 1 cut(s) 804
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 3 cut(s) 311, 436, 533
HapII CCGG 2 cut(s) 518, 741
HhaI GCGC 1 cut(s) 639
Hin1II CATG 4 cut(s) 324, 490, 527, 810
Hin6I GCGC 1 cut(s) 637
HinP1I GCGC 1 cut(s) 637
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 5 cut(s) 101, 113, 215, 676, 703
HpaII CCGG 2 cut(s) 518, 741
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 121, 646
Hpy188I TCNGA 2 cut(s) 160, 722
Hpy8I GTNNAC 2 cut(s) 121, 646
HpyAV CCTTC 6 cut(s) 22, 79, 287, 320, 392, 617
HpyCH4III ACNGT 2 cut(s) 472, 575
HpyCH4V TGCA 5 cut(s) 176, 508, 608, 663, 672
HpyF10VI GCNNNNNNNGC 2 cut(s) 514, 669
HpyF3I CTNAG 2 cut(s) 399, 719
Hsp92II CATG 4 cut(s) 324, 490, 527, 810
HspAI GCGC 1 cut(s) 637
Kpn2I TCCGGA 1 cut(s) 740
Kzo9I GATC 5 cut(s) 58, 334, 520, 563, 746
LmnI GCTCC 2 cut(s) 355, 760
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 2 cut(s) 325, 495
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 338
MalI GATC 5 cut(s) 60, 336, 522, 565, 748
MboI GATC 5 cut(s) 58, 334, 520, 563, 746
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 5 cut(s) 93, 297, 481, 616, 759
MlyI GAGTC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 510
MroI TCCGGA 1 cut(s) 740
MseI TTAA 2 cut(s) 480, 762
MspI CCGG 2 cut(s) 518, 741
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 514, 669
NcoI CCATGG 1 cut(s) 320
NdeII GATC 5 cut(s) 58, 334, 520, 563, 746
NlaIII CATG 4 cut(s) 324, 490, 527, 810
NlaIV GGNNCC 4 cut(s) 39, 310, 565, 739
NmuCI GTSAC 1 cut(s) 338
NsiI ATGCAT 1 cut(s) 510
PceI AGGCCT 1 cut(s) 436
PcsI WCGNNNNNNNCGW 1 cut(s) 787
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 101, 113, 215, 703
PkrI GCNGC 1 cut(s) 516
PleI GAGTC 1 cut(s) 684
PpsI GAGTC 1 cut(s) 684
PspFI CCCAGC 1 cut(s) 800
PspN4I GGNNCC 4 cut(s) 39, 310, 565, 739
PspPI GGNCC 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 563
RsaI GTAC 2 cut(s) 64, 447
RsaNI GTAC 2 cut(s) 63, 446
SaqAI TTAA 2 cut(s) 480, 762
SatI GCNGC 1 cut(s) 515
Sau3AI GATC 5 cut(s) 58, 334, 520, 563, 746
Sau96I GGNCC 1 cut(s) 309
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 1 cut(s) 685
SduI GDGCHC 1 cut(s) 461
SetI ASST 7 cut(s) 39, 90, 150, 360, 405, 587, 769
SfaNI GCATC 2 cut(s) 325, 495
Sse9I AATT 5 cut(s) 93, 297, 481, 616, 759
SseBI AGGCCT 1 cut(s) 436
SspMI CTAG 1 cut(s) 149
StuI AGGCCT 1 cut(s) 436
StyI CCWWGG 2 cut(s) 320, 696
TaaI ACNGT 2 cut(s) 472, 575
TasI AATT 5 cut(s) 93, 297, 481, 616, 759
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 4 cut(s) 101, 113, 215, 703
Tru1I TTAA 2 cut(s) 480, 762
Tru9I TTAA 2 cut(s) 480, 762
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 3 cut(s) 246, 297, 475
TspRI CASTG 1 cut(s) 226
XapI RAATTY 1 cut(s) 616
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.