RchiOBHm_Chr5g0073521

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007022
Physical Location & Seq
Reverse (-)
79513266 .. 79516023
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34840

Sequence Viewer

Length: 1020 bp
ATGGCTGAAGAGAACAAGAATGCTGTGAAGGAGGAGGTTCCAGATATTGTCCCCTTTGATCGTACTAAAAAGAAGAAAAAGAAGAAGGTGGTAATTCGGGATTCTTCAAGAGATTCTGTGGACAAGTTGGCTGACAAAACAGAAAACCTAGCAGGTAGTTTAAGCGATTCTCTTCCAACTGTACTATACATCCATCACTTTTGTGAAGGACTTGTGTTTTTCCTCTTTGCAGTTTCTGATGGACAGGAAAGTGCATTTTCTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAATCCAGTGTCTTGAATGAAGAGGGTGGTGATACAAGGGAAGATTTGGATGACGGGACTGGGGATGATGAAGAGGGAGAAGGAATTGTTTTAGGGGCCTCGCATCCATGGGAAGGGAGTGATCGTGACTACCACTATGAGGAGCTTCTTGGTAGGGTTTTCAATATCCTTCGGGAGAATAATCCTGAGCTGGCTGGCGACAGGCGTAGGACAACTATGAGGCCTCCACAAGTACTTCGTGAGGGCACAAAGAAAACTGTCTTTGTTAATTTCATGGACTTATGTGGTCATACTGAAAAAGATCACTATGTGGTGTTAACATGGTTTTCCTTTCTTGTAAAAGTGGTTTTTTTTTGCTGTTTATTTGAGTATCCAACTATATGGACTACTGTAAATCTACACTTCACAAGAGGATTATGGAAGATGCATAGGCAGCCGGATCATGTTATGGCCTTTTTACTGGCTGAACTGGGGACAAGTGGATCCCTTGACGGTCAGCAGAGGTTAGTTGTAAAGGGAAGATTTGCACCCAAGAATTTTGAAGGAATACTACGGCGCTATGTCAACGAATATGTCATTTGCATTGGGTGCAAGAGTCCTGACACTATACTGTCCAAGGAGAATCGCCTCTTTTTTCTCAGATGTGAGAAGTGTGGTTCGGGACGATCTGTTGCTCCAATTAAAGCTGGTTTTGTTGCTCGTGTTGGGCGAAGAAATGCTGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

339

Amino Acids

38.37

Weight (kDa)

8.32

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 235 - 317 2.6e-28 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
AclWI GGATC 3 cut(s) 738, 768, 781
AcsI RAATTY 1 cut(s) 826
AcuI CTGAAG 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 3 cut(s) 64, 183, 525
AfiI CCNNNNNNNGG 2 cut(s) 404, 430
AgsI TTSAA 6 cut(s) 108, 268, 293, 307, 454, 833
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 3 cut(s) 436, 481, 977
AluI AGCT 3 cut(s) 436, 481, 977
AlwI GGATC 3 cut(s) 738, 768, 781
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 3 cut(s) 387, 512, 741
ApeKI GCWGC 1 cut(s) 724
ApoI RAATTY 1 cut(s) 826
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 1 cut(s) 387
AsuHPI GGTGA 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 539
BamHI GGATCC 1 cut(s) 773
BarI GAAGNNNNNNTAC 2 cut(s) 825, 857
BauI CACGAG 1 cut(s) 990
BbvI GCAGC 1 cut(s) 736
BccI CCATC 2 cut(s) 201, 233
BceAI ACGGC 1 cut(s) 860
BciVI GTATCC 1 cut(s) 672
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 850
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 1 cut(s) 672
BisI GCNGC 1 cut(s) 725
BlsI GCNGC 1 cut(s) 726
BmcAI AGTACT 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 387
BmiI GGNNCC 3 cut(s) 39, 388, 775
BmrI ACTGGG 2 cut(s) 360, 770
BmsI GCATC 2 cut(s) 403, 705
BmuI ACTGGG 2 cut(s) 360, 770
Bpu10I CCTNAGC 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 398, 906
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 430
Bse1I ACTGG 5 cut(s) 287, 297, 355, 756, 765
BseDI CCNNGG 2 cut(s) 398, 906
BseGI GGATG 4 cut(s) 189, 346, 361, 394
BseLI CCNNNNNNNGG 2 cut(s) 404, 430
BseMII CTCAG 2 cut(s) 468, 943
BseNI ACTGG 5 cut(s) 287, 297, 355, 756, 765
BseRI GAGGAG 2 cut(s) 47, 446
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 1 cut(s) 736
BseYI CCCAGC 1 cut(s) 1010
BshFI GGCC 3 cut(s) 389, 514, 743
BsiSI CCGG 1 cut(s) 728
BslFI GGGAC 4 cut(s) 35, 361, 778, 966
BslI CCNNNNNNNGG 2 cut(s) 404, 430
BsmFI GGGAC 4 cut(s) 35, 361, 778, 966
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 3 cut(s) 389, 514, 743
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 6 cut(s) 58, 412, 592, 730, 773, 956
Bsp19I CCATGG 1 cut(s) 398
BspANI GGCC 3 cut(s) 389, 514, 743
BspCNI CTCAG 2 cut(s) 469, 942
BspLI GGNNCC 3 cut(s) 39, 388, 775
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 738, 768, 781
BsrI ACTGG 5 cut(s) 287, 297, 355, 756, 765
BssECI CCNNGG 2 cut(s) 398, 906
BssMI GATC 6 cut(s) 58, 412, 592, 730, 773, 956
BssSI CACGAG 1 cut(s) 990
BssT1I CCWWGG 2 cut(s) 398, 906
Bst2BI CACGAG 1 cut(s) 990
Bst4CI ACNGT 5 cut(s) 181, 550, 682, 785, 903
Bst6I CTCTTC 4 cut(s) 3, 177, 306, 357
BstAPI GCANNNNNTGC 1 cut(s) 879
BstC8I GCNNGC 2 cut(s) 483, 487
BstDEI CTNAG 2 cut(s) 477, 929
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 4 cut(s) 189, 346, 361, 394
BstH2I RGCGCY 1 cut(s) 850
BstHHI GCGC 1 cut(s) 849
BstKTI GATC 6 cut(s) 61, 415, 595, 733, 776, 959
BstMBI GATC 6 cut(s) 58, 412, 592, 730, 773, 956
BstMWI GCNNNNNNNGC 2 cut(s) 724, 879
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 736
BstX2I RGATCY 1 cut(s) 773
BstXI CCANNNNNNTGG 1 cut(s) 672
BstYI RGATCY 1 cut(s) 773
BsuI GTATCC 1 cut(s) 672
BsuRI GGCC 3 cut(s) 389, 514, 743
BtgI CCRYGG 1 cut(s) 398
BtsCI GGATG 4 cut(s) 189, 346, 361, 394
BtsIMutI CAGTG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 483, 487
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 1 cut(s) 849
Cfr13I GGNCC 1 cut(s) 387
Csp6I GTAC 3 cut(s) 63, 182, 524
CviAII CATG 5 cut(s) 399, 565, 612, 734, 1017
CviQI GTAC 3 cut(s) 63, 182, 524
DdeI CTNAG 2 cut(s) 477, 929
DpnI GATC 6 cut(s) 60, 414, 594, 732, 775, 958
DpnII GATC 6 cut(s) 58, 412, 592, 730, 773, 956
DraIII CACNNNGTG 1 cut(s) 601
Eam1104I CTCTTC 4 cut(s) 3, 177, 306, 357
EarI CTCTTC 4 cut(s) 3, 177, 306, 357
Eco130I CCWWGG 2 cut(s) 398, 906
Eco147I AGGCCT 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 398, 906
EcoT22I ATGCAT 1 cut(s) 720
ErhI CCWWGG 2 cut(s) 398, 906
FaeI CATG 5 cut(s) 402, 568, 615, 737, 1020
FaqI GGGAC 4 cut(s) 35, 361, 778, 966
FatI CATG 5 cut(s) 398, 564, 611, 733, 1016
Fnu4HI GCNGC 1 cut(s) 725
FokI GGATG 4 cut(s) 176, 353, 368, 381
Fsp4HI GCNGC 1 cut(s) 725
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 848
GluI GCNGC 1 cut(s) 725
GsaI CCCAGC 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 850
HaeIII GGCC 3 cut(s) 389, 514, 743
HapII CCGG 1 cut(s) 728
HhaI GCGC 1 cut(s) 849
Hin1II CATG 5 cut(s) 402, 568, 615, 737, 1020
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HincII GTYRAC 2 cut(s) 609, 856
HindII GTYRAC 2 cut(s) 609, 856
HinfI GANTC 6 cut(s) 101, 113, 167, 293, 886, 913
HpaI GTTAAC 1 cut(s) 609
HpaII CCGG 1 cut(s) 728
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 3 cut(s) 121, 609, 856
Hpy188I TCNGA 2 cut(s) 238, 932
Hpy8I GTNNAC 3 cut(s) 121, 609, 856
HpyAV CCTTC 7 cut(s) 22, 79, 200, 365, 398, 470, 827
HpyCH4III ACNGT 5 cut(s) 181, 550, 682, 785, 903
HpyCH4V TGCA 6 cut(s) 230, 254, 718, 818, 873, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 724, 879
HpyF3I CTNAG 2 cut(s) 477, 929
Hsp92II CATG 5 cut(s) 402, 568, 615, 737, 1020
HspAI GCGC 1 cut(s) 847
KspAI GTTAAC 1 cut(s) 609
Kzo9I GATC 6 cut(s) 58, 412, 592, 730, 773, 956
LmnI GCTCC 2 cut(s) 433, 970
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 2 cut(s) 403, 705
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 416
MalI GATC 6 cut(s) 60, 414, 594, 732, 775, 958
MboI GATC 6 cut(s) 58, 412, 592, 730, 773, 956
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 5 cut(s) 93, 375, 559, 826, 969
MlyI GAGTC 1 cut(s) 895
MmeI TCCRAC 2 cut(s) 200, 689
Mph1103I ATGCAT 1 cut(s) 720
MseI TTAA 4 cut(s) 161, 558, 608, 972
MslI CAYNNNNRTG 1 cut(s) 201
MspI CCGG 1 cut(s) 728
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 724, 879
NcoI CCATGG 1 cut(s) 398
NdeII GATC 6 cut(s) 58, 412, 592, 730, 773, 956
NlaIII CATG 5 cut(s) 402, 568, 615, 737, 1020
NlaIV GGNNCC 3 cut(s) 39, 388, 775
NmuCI GTSAC 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 720
OliI CACNNNNGTG 1 cut(s) 201
PceI AGGCCT 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 5 cut(s) 101, 113, 167, 293, 913
PkrI GCNGC 1 cut(s) 726
PleI GAGTC 1 cut(s) 894
PpsI GAGTC 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 1010
PspN4I GGNNCC 3 cut(s) 39, 388, 775
PspPI GGNCC 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 773
RsaI GTAC 3 cut(s) 64, 183, 525
RsaNI GTAC 3 cut(s) 63, 182, 524
RseI CAYNNNNRTG 1 cut(s) 201
SaqAI TTAA 4 cut(s) 161, 558, 608, 972
SatI GCNGC 1 cut(s) 725
Sau3AI GATC 6 cut(s) 58, 412, 592, 730, 773, 956
Sau96I GGNCC 1 cut(s) 387
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 1 cut(s) 895
SduI GDGCHC 1 cut(s) 539
SetI ASST 8 cut(s) 39, 90, 150, 157, 438, 483, 797, 979
SfaNI GCATC 2 cut(s) 403, 705
SmiMI CAYNNNNRTG 1 cut(s) 201
Sse9I AATT 5 cut(s) 93, 375, 559, 826, 969
SseBI AGGCCT 1 cut(s) 514
SspMI CTAG 1 cut(s) 149
StuI AGGCCT 1 cut(s) 514
StyI CCWWGG 2 cut(s) 398, 906
TaaI ACNGT 5 cut(s) 181, 550, 682, 785, 903
TasI AATT 5 cut(s) 93, 375, 559, 826, 969
TatI WGTACW 2 cut(s) 181, 523
TfiI GAWTC 5 cut(s) 101, 113, 167, 293, 913
Tru1I TTAA 4 cut(s) 161, 558, 608, 972
Tru9I TTAA 4 cut(s) 161, 558, 608, 972
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 1 cut(s) 724
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 3 cut(s) 324, 375, 553
TspRI CASTG 1 cut(s) 304
XapI RAATTY 1 cut(s) 826
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.