Rorug02G0164600

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007022
Physical Location & Seq
Forward (+)
14669520 .. 14670853
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0164600.1

Sequence Viewer

Length: 912 bp
ATGCATAATTTAAAGGATTATTGTAGAAACAAAGCCCATCCCGAAGGATCAATTGCTGAAGGTTATTGCGACAGTGAATGCCTCACAATTTGTTCTATGTACTTTCGCGACATTGAGACAAAATTTAACCAAGGAGACAGGAATCATGATGTGAGTGAGAGAAGAATGACAGGAATATCTGTTTTCAACCAGAATGTTAGGTTTTTAAAAGGAGCAGTTGATGATGTTCTTAGCCTAACTGATTTTGCAATGATTCGATGGTATGTGCTGAACAATTGTGATGAGGTACTCCCATATATAAGGGAGCATAAAGCAGAACTTGAGAGGCAGAATATTACAAACATAGGAAAGGAACAGCAACAAAGATTTCATAAGTGGTTTTTGAGACGTGTTCAACAGATGCAAGTTGAGGGTTCAACAGAACATATCGAATCCTTACTGAACTTGGCCAGTGGACCTCAGCGTGAAGTGACACGGTATAGTGGGTGTGTTGTCAATGGCATAAGGTGGTCGTTTTCAAGTGTGATTGGCTGGACCTCAATCCAAGAAAGGGAATTCAAGTTGACGAGAACCATTTTACAAGCGTCAATTTTACCAAAAAATGAAAGGTCACAACCTCGAGGAACTTATGATTTTACTGAAAAAGAAGCTGAAGTGGATGATTTATTAGAGGAGATTGAAGATCCATATCAACAAGAGTCACATGAGTATGTCAATTTAGATGAAATTGACATTGGGGAGACTTCATTGTGTAGAGATGACAGTGAAATGATCATTGTTGATTTGAATGCTGACAAGCAAGGGATTAGTGATTCTACATTCATCAATGCTAGCGATGCAGAAGATGGTGACTTTGGTGATGAAGATAATGAGTTTATGTATTCTGATGATAACGAGGACGACTATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

303

Amino Acids

35.2

Weight (kDa)

4.49

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4218 PF13960 2 - 48 5.3e-14 Domain of unknown function (DUF4218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 618
AccII CGCG 1 cut(s) 108
AclWI GGATC 2 cut(s) 55, 677
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 2 cut(s) 122, 554
AcuI CTGAAG 2 cut(s) 78, 672
AfaI GTAC 2 cut(s) 101, 288
AfiI CCNNNNNNNGG 1 cut(s) 550
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 7 cut(s) 187, 395, 417, 519, 559, 680, 787
AjiI CACGTC 1 cut(s) 389
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AluBI AGCT 1 cut(s) 650
AluI AGCT 1 cut(s) 650
Alw26I GTCTC 4 cut(s) 110, 129, 379, 734
AlwI GGATC 2 cut(s) 55, 677
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 1 cut(s) 447
ApoI RAATTY 2 cut(s) 122, 554
AspS9I GGNCC 2 cut(s) 455, 534
AsuHPI GGTGA 2 cut(s) 860, 869
AsuNHI GCTAGC 1 cut(s) 830
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 2 cut(s) 455, 534
BalI TGGCCA 1 cut(s) 449
BbvCI CCTCAGC 1 cut(s) 459
BccI CCATC 3 cut(s) 45, 252, 839
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 4 cut(s) 110, 129, 379, 734
BfaI CTAG 1 cut(s) 831
Bme18I GGWCC 2 cut(s) 455, 534
BmeT110I CYCGRG 1 cut(s) 618
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 455, 534
BmsI GCATC 2 cut(s) 390, 826
BmtI GCTAGC 1 cut(s) 834
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 687
BsaJI CCNNGG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 687
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 37, 664
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 686
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 618
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 4 cut(s) 110, 129, 379, 734
BsmBI CGTCTC 1 cut(s) 379
BsmI GAATGC 2 cut(s) 83, 793
BsnI GGCC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 618
Bsp143I GATC 3 cut(s) 47, 682, 771
Bsp68I TCGCGA 1 cut(s) 108
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 1 cut(s) 472
BspFNI CGCG 1 cut(s) 108
BspHI TCATGA 1 cut(s) 145
BspOI GCTAGC 1 cut(s) 834
BspPI GGATC 2 cut(s) 55, 677
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 3 cut(s) 47, 682, 771
BssT1I CCWWGG 1 cut(s) 130
Bst4CI ACNGT 3 cut(s) 74, 477, 764
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 2 cut(s) 230, 459
BstF5I GGATG 2 cut(s) 37, 664
BstFNI CGCG 1 cut(s) 108
BstKTI GATC 3 cut(s) 50, 685, 774
BstMAI GTCTC 4 cut(s) 110, 129, 379, 734
BstMBI GATC 3 cut(s) 47, 682, 771
BstMWI GCNNNNNNNGC 1 cut(s) 836
BstUI CGCG 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
BsuRI GGCC 1 cut(s) 449
BtgZI GCGATG 1 cut(s) 849
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 37, 664
BtsIMutI CAGTG 3 cut(s) 79, 457, 769
BtuMI TCGCGA 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 832
CciI TCATGA 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 455, 534
CseI GACGC 1 cut(s) 573
Csp6I GTAC 2 cut(s) 100, 287
CviAII CATG 2 cut(s) 146, 704
CviJI RGCY 5 cut(s) 35, 234, 449, 531, 650
CviKI_1 RGCY 5 cut(s) 35, 234, 449, 531, 650
CviQI GTAC 2 cut(s) 100, 287
DdeI CTNAG 2 cut(s) 230, 459
DpnI GATC 3 cut(s) 49, 684, 773
DpnII GATC 3 cut(s) 47, 682, 771
DraI TTTAAA 2 cut(s) 12, 207
EaeI YGGCCR 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 455, 534
Eco57I CTGAAG 2 cut(s) 78, 672
Eco88I CYCGRG 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 554
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 2 cut(s) 149, 707
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 2 cut(s) 145, 703
FbaI TGATCA 1 cut(s) 771
FokI GGATG 2 cut(s) 24, 671
FspBI CTAG 1 cut(s) 831
HaeIII GGCC 1 cut(s) 449
HgaI GACGC 1 cut(s) 573
Hin1II CATG 2 cut(s) 149, 707
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HinfI GANTC 5 cut(s) 142, 253, 431, 698, 812
HphI GGTGA 2 cut(s) 860, 869
Hpy166II GTNNAC 2 cut(s) 455, 564
Hpy188I TCNGA 1 cut(s) 886
Hpy188III TCNNGA 3 cut(s) 41, 107, 146
Hpy8I GTNNAC 2 cut(s) 455, 564
HpyAV CCTTC 2 cut(s) 38, 53
HpyCH4III ACNGT 3 cut(s) 74, 477, 764
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 4 cut(s) 4, 248, 403, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 836
HpyF3I CTNAG 2 cut(s) 230, 459
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 2 cut(s) 149, 707
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 3 cut(s) 47, 682, 771
LmnI GCTCC 2 cut(s) 212, 304
LpnPI CCDG 5 cut(s) 124, 156, 203, 463, 517
LweI GCATC 2 cut(s) 390, 826
MaeI CTAG 1 cut(s) 831
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 4 cut(s) 469, 609, 699, 848
MalI GATC 3 cut(s) 49, 684, 773
MboI GATC 3 cut(s) 47, 682, 771
MboII GAAGA 4 cut(s) 174, 692, 854, 875
MfeI CAATTG 2 cut(s) 51, 274
MflI RGATCY 1 cut(s) 682
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 9 cut(s) 7, 51, 87, 122, 274, 554, 588, 715, 726
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 1 cut(s) 707
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 449
MseI TTAA 3 cut(s) 11, 126, 206
MslI CAYNNNNRTG 1 cut(s) 708
Msp20I TGGCCA 1 cut(s) 449
MunI CAATTG 2 cut(s) 51, 274
Mva1269I GAATGC 2 cut(s) 83, 793
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 836
NdeII GATC 3 cut(s) 47, 682, 771
NheI GCTAGC 1 cut(s) 830
NlaIII CATG 2 cut(s) 149, 707
NmuCI GTSAC 4 cut(s) 469, 609, 699, 848
NruI TCGCGA 1 cut(s) 108
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 618
PagI TCATGA 1 cut(s) 145
PctI GAATGC 2 cut(s) 83, 793
PfeI GAWTC 4 cut(s) 142, 253, 431, 812
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PspPI GGNCC 2 cut(s) 455, 534
PspXI VCTCGAGB 1 cut(s) 618
PsuI RGATCY 1 cut(s) 682
RruI TCGCGA 1 cut(s) 108
RsaI GTAC 2 cut(s) 101, 288
RsaNI GTAC 2 cut(s) 100, 287
RseI CAYNNNNRTG 1 cut(s) 708
SaqAI TTAA 3 cut(s) 11, 126, 206
Sau3AI GATC 3 cut(s) 47, 682, 771
Sau96I GGNCC 2 cut(s) 455, 534
SchI GAGTC 1 cut(s) 707
SfaNI GCATC 2 cut(s) 390, 826
Sfr274I CTCGAG 1 cut(s) 618
SinI GGWCC 2 cut(s) 455, 534
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 2 cut(s) 320, 618
SmoI CTYRAG 2 cut(s) 320, 618
Sse9I AATT 9 cut(s) 7, 51, 87, 122, 274, 554, 588, 715, 726
SspI AATATT 1 cut(s) 334
SspMI CTAG 1 cut(s) 831
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 3 cut(s) 74, 477, 764
TaiI ACGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 256, 429, 619
TasI AATT 9 cut(s) 7, 51, 87, 122, 274, 554, 588, 715, 726
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 4 cut(s) 142, 253, 431, 812
Tru1I TTAA 3 cut(s) 11, 126, 206
Tru9I TTAA 3 cut(s) 11, 126, 206
TscAI CASTG 3 cut(s) 79, 457, 769
TseFI GTSAC 4 cut(s) 469, 609, 699, 848
Tsp45I GTSAC 4 cut(s) 469, 609, 699, 848
TspDTI ATGAA 6 cut(s) 359, 618, 735, 738, 811, 876
TspRI CASTG 3 cut(s) 79, 457, 769
VpaK11BI GGWCC 2 cut(s) 455, 534
XapI RAATTY 2 cut(s) 122, 554
XhoI CTCGAG 1 cut(s) 618
XspI CTAG 1 cut(s) 831
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.