Rorug05G0424300

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007022
Physical Location & Seq
Forward (+)
58957485 .. 58958252
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0424300.1

Sequence Viewer

Length: 768 bp
ATGTCCAATTCTTTATTGGCTCAGGTATGCTATAGCATTCATAATGTACATGATGATGCGTTGGGCTGGTTAGAGCGGTGTGCTCAGGAACTTTCTCTAGCTCATTTTGGGGAGCTTCTGTTTGCTCTCTGGGGGGTCTGGACGGAGAGGAATGATAGAGTTTGGAATAATAAGCAGCGTGAGGCAGCTGATGTGTGTTTGGGACTTGTTTCGAGGTTACAGGAATTTAGGTTCCATAATGTGAAGCATGTTGTTCCTACGAGTAGGAGGAAGGCAGTTTGGAGAGCTCCACCGATGGGAATTGTGAAAATAAATGTGGATGGATCATATAATCATTTGACTCGTCAAGGAGGTGTAGGCTTTGTGATTCGGGATGATCGAGGGGTAATGTTAGCAGGAGGAGCCTGGCCGGTGCGGGGTTTATACTCGCCTGAGCATGCGGAAATCTTAGCATGTAAATATGCTTTGCAGTTTGCTTTGGACCAAGATTTTGGACCCTTTGTCTTGGAAACAGACGCAATGGAAGTGCAAAGGCAACTCTCGATGAACGGTAAAGCCAACACTTCGGTGCTGGGCCGTATTTTTGATGATGTGGTTTTGCTCTTGGAGTCCCAGTCTACATTGGGAGTGAAGCACATAGGGAGAAGTGGAAATATGGTTGCCCATCAGATGGCCATGCATGCTTGTTCTCTTGAACAAGAACAATTTTTCTTTTCTGCACCTTCTTTTCTGTTGGCTGCAATTGCAGCAGATCATTGTACTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

255

Amino Acids

28.47

Weight (kDa)

6.4

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 105 - 228 1.2e-22 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 670
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 617
AciI CCGC 3 cut(s) 76, 415, 440
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 2 cut(s) 407, 672
AcsI RAATTY 1 cut(s) 224
AfaI GTAC 2 cut(s) 48, 760
AfiI CCNNNNNNNGG 3 cut(s) 296, 416, 670
AgsI TTSAA 1 cut(s) 695
AhdI GACNNNNNGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 404
AleI CACNNNNGTG 1 cut(s) 566
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 4 cut(s) 101, 115, 188, 287
AluI AGCT 4 cut(s) 101, 115, 188, 287
Alw21I GWGCWC 2 cut(s) 85, 289
AlwI GGATC 1 cut(s) 331
AoxI GGCC 3 cut(s) 407, 574, 672
ApeKI GCWGC 4 cut(s) 175, 185, 737, 746
ApoI RAATTY 1 cut(s) 224
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspS9I GGNCC 3 cut(s) 481, 494, 574
AvaII GGWCC 2 cut(s) 481, 494
BalI TGGCCA 1 cut(s) 674
BanII GRGCYC 1 cut(s) 289
Bbv12I GWGCWC 2 cut(s) 85, 289
BbvI GCAGC 4 cut(s) 187, 197, 724, 758
BccI CCATC 4 cut(s) 289, 314, 664, 672
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 1 cut(s) 406
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 31
BisI GCNGC 4 cut(s) 176, 186, 738, 747
BlsI GCNGC 4 cut(s) 177, 187, 739, 748
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 2 cut(s) 481, 494
BmeRI GACNNNNNGTC 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 481, 494, 574
BmiI GGNNCC 3 cut(s) 233, 403, 496
BmrFI CCNGG 1 cut(s) 406
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 607
Bpu10I CCTNAGC 3 cut(s) 21, 84, 432
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 599, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 416, 670
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 1 cut(s) 406
BseGI GGATG 2 cut(s) 325, 379
BseLI CCNNNNNNNGG 3 cut(s) 296, 416, 670
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 3 cut(s) 35, 98, 423
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 1 cut(s) 414
BseXI GCAGC 4 cut(s) 187, 197, 724, 758
BseYI CCCAGC 1 cut(s) 571
BsgI GTGCAG 1 cut(s) 702
BshFI GGCC 3 cut(s) 409, 576, 674
BsiHKAI GWGCWC 2 cut(s) 85, 289
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 2 cut(s) 216, 595
BslI CCNNNNNNNGG 3 cut(s) 296, 416, 670
BsmFI GGGAC 2 cut(s) 216, 595
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 3 cut(s) 409, 576, 674
Bsp1286I GDGCHC 2 cut(s) 85, 289
Bsp1407I TGTACA 1 cut(s) 46
Bsp143I GATC 3 cut(s) 323, 376, 751
BspACI CCGC 3 cut(s) 76, 415, 440
BspANI GGCC 3 cut(s) 409, 576, 674
BspCNI CTCAG 3 cut(s) 34, 97, 424
BspLI GGNNCC 3 cut(s) 233, 403, 496
BspPI GGATC 1 cut(s) 331
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 409
BsrGI TGTACA 1 cut(s) 46
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 409
BssMI GATC 3 cut(s) 323, 376, 751
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 438, 681
BstDEI CTNAG 4 cut(s) 21, 84, 432, 448
BstF5I GGATG 2 cut(s) 325, 379
BstKTI GATC 3 cut(s) 326, 379, 754
BstMBI GATC 3 cut(s) 323, 376, 751
BstMWI GCNNNNNNNGC 4 cut(s) 401, 680, 743, 746
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 4 cut(s) 251, 440, 456, 683
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 31
BstV1I GCAGC 4 cut(s) 187, 197, 724, 758
BstXI CCANNNNNNTGG 1 cut(s) 491
BsuRI GGCC 3 cut(s) 409, 576, 674
BtsCI GGATG 2 cut(s) 325, 379
Cac8I GCNNGC 2 cut(s) 438, 681
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 3 cut(s) 481, 494, 574
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 47, 759
CviAII CATG 6 cut(s) 50, 248, 437, 453, 676, 680
CviQI GTAC 2 cut(s) 47, 759
DdeI CTNAG 4 cut(s) 21, 84, 432, 448
DpnI GATC 3 cut(s) 325, 378, 753
DpnII GATC 3 cut(s) 323, 376, 751
DriI GACNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 2 cut(s) 407, 672
Eam1105I GACNNNNNGTC 1 cut(s) 500
Ecl136II GAGCTC 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 481, 494
Eco53kI GAGCTC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 681
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 6 cut(s) 53, 251, 440, 456, 679, 683
FaqI GGGAC 2 cut(s) 216, 595
FatI CATG 6 cut(s) 49, 247, 436, 452, 675, 679
FauI CCCGC 1 cut(s) 408
FblI GTMKAC 1 cut(s) 617
Fnu4HI GCNGC 4 cut(s) 176, 186, 738, 747
FokI GGATG 2 cut(s) 332, 386
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 4 cut(s) 176, 186, 738, 747
FspBI CTAG 1 cut(s) 98
GluI GCNGC 4 cut(s) 176, 186, 738, 747
GsaI CCCAGC 1 cut(s) 575
HaeIII GGCC 3 cut(s) 409, 576, 674
HapII CCGG 1 cut(s) 410
HgaI GACGC 1 cut(s) 524
Hin1II CATG 6 cut(s) 53, 251, 440, 456, 679, 683
HinfI GANTC 3 cut(s) 340, 367, 608
HpaII CCGG 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 618
Hpy188I TCNGA 1 cut(s) 669
Hpy188III TCNNGA 5 cut(s) 86, 139, 371, 541, 692
Hpy8I GTNNAC 1 cut(s) 618
HpyAV CCTTC 2 cut(s) 265, 732
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4V TGCA 6 cut(s) 469, 529, 679, 719, 740, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 401, 680, 743, 746
HpyF3I CTNAG 4 cut(s) 21, 84, 432, 448
Hsp92II CATG 6 cut(s) 53, 251, 440, 456, 679, 683
Kzo9I GATC 3 cut(s) 323, 376, 751
LmnI GCTCC 3 cut(s) 112, 292, 401
Lsp1109I GCAGC 4 cut(s) 187, 197, 724, 758
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 216
MalI GATC 3 cut(s) 325, 378, 753
MbiI CCGCTC 1 cut(s) 76
MboI GATC 3 cut(s) 323, 376, 751
MfeI CAATTG 1 cut(s) 741
MhlI GDGCHC 2 cut(s) 85, 289
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 5 cut(s) 7, 224, 300, 704, 741
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 2 cut(s) 334, 617
MnlI CCTC 7 cut(s) 141, 175, 207, 261, 344, 374, 392
Mox20I TGGCCA 1 cut(s) 674
Mph1103I ATGCAT 1 cut(s) 681
MscI TGGCCA 1 cut(s) 674
MseI TTAA 1 cut(s) 766
MslI CAYNNNNRTG 2 cut(s) 54, 566
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 1 cut(s) 406
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 4 cut(s) 401, 680, 743, 746
NdeII GATC 3 cut(s) 323, 376, 751
NlaIII CATG 6 cut(s) 53, 251, 440, 456, 679, 683
NlaIV GGNNCC 3 cut(s) 233, 403, 496
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 4 cut(s) 251, 440, 456, 683
OliI CACNNNNGTG 1 cut(s) 566
PaeI GCATGC 2 cut(s) 440, 683
PcsI WCGNNNNNNNCGW 1 cut(s) 376
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 367
PflMI CCANNNNNTGG 1 cut(s) 670
PkrI GCNGC 4 cut(s) 177, 187, 739, 748
PleI GAGTC 2 cut(s) 334, 616
PpsI GAGTC 2 cut(s) 334, 616
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 3 cut(s) 233, 403, 496
PspPI GGNCC 3 cut(s) 481, 494, 574
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 48, 760
RsaNI GTAC 2 cut(s) 47, 759
RseI CAYNNNNRTG 2 cut(s) 54, 566
SacI GAGCTC 1 cut(s) 289
SaqAI TTAA 1 cut(s) 766
SatI GCNGC 4 cut(s) 176, 186, 738, 747
Sau3AI GATC 3 cut(s) 323, 376, 751
Sau96I GGNCC 3 cut(s) 481, 494, 574
SchI GAGTC 2 cut(s) 334, 617
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 2 cut(s) 85, 289
SetI ASST 9 cut(s) 27, 103, 117, 190, 218, 233, 289, 355, 724
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 31
SinI GGWCC 2 cut(s) 481, 494
SmiMI CAYNNNNRTG 2 cut(s) 54, 566
SphI GCATGC 2 cut(s) 440, 683
Sse9I AATT 5 cut(s) 7, 224, 300, 704, 741
SsiI CCGC 3 cut(s) 76, 415, 440
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 551
TaqI TCGA 3 cut(s) 212, 379, 542
TasI AATT 5 cut(s) 7, 224, 300, 704, 741
TatI WGTACW 2 cut(s) 46, 758
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 1 cut(s) 766
Tru9I TTAA 1 cut(s) 766
TseI GCWGC 4 cut(s) 175, 185, 737, 746
TspDTI ATGAA 2 cut(s) 29, 560
TspGWI ACGGA 1 cut(s) 158
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 2 cut(s) 481, 494
XapI RAATTY 1 cut(s) 224
XceI RCATGY 4 cut(s) 251, 440, 456, 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 617
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.