Rroxscaffold_1G00007750

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007022
Physical Location & Seq
Forward (+)
9901444 .. 9904805
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007750.1

Sequence Viewer

Length: 735 bp
ATGGCTGAAGAGAACAAGAATGCTGTGAAGGAGGAGGTTCCAGATATTGTCCCCTTTGATCGTACTAAAAAGAAGAAAAAGAAGAAGGTGGTAATTCGGGATTCTTCAAGCGATTCTGTGGACAAGTTGGCTGACAAAACAGAAAACCTAGCAGGTAGTTTAAGCGAACCATTGATTACCCTTGATGAGGTTTATGTTGTTGGCAATTTAGACGGGACTGGGGATGATGAAGAGGGAGAAGGAATTGTTTTAGGGGCCTCGCATCCATGGGAAGGAGTGACCCTTCTTGGTAGGGTGTTCAATATCCTTCGGGAGAATAATCCTGAGCTGGCTGGCGACAGGCGTAGGACAACTATGAGGCCTCCACAAGTACTTCGTGAGGGCACAAAGAAAACTGTCTTTGTTAATTTCATGGACTTATGTAAGACGATGCATAGGCAGCCGGATCATGTTATGGCCTTTTTACTGGCTGAACTGGGGACAAGTGGATCCCTTGACGGTCAGCAGAGGTTAGTTGTAAAGGGAAGATTTGCACCCAAGAATTTTGAAGGAATACTACGGCGCTATGTCAACGAATATGTCATTTGCATTGGGTGCAAGAGTCCTGACACTATACTCTCCAAGGAGAATCGCCTCTTCTTTCTCAGATGCGAGAAGTGTGGTTCCGGACGATCTGTTGCTCCAATTAAAGCTGGTTTTGTTGCTCGTGTTGGGCGAAGAAATGCTGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

244

Amino Acids

27.06

Weight (kDa)

9.05

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 113 - 222 4.4e-40 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 665
AclWI GGATC 3 cut(s) 453, 483, 496
AcsI RAATTY 1 cut(s) 541
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 2 cut(s) 64, 372
AfiI CCNNNNNNNGG 2 cut(s) 187, 272
AgsI TTSAA 3 cut(s) 108, 301, 548
AluBI AGCT 2 cut(s) 328, 692
AluI AGCT 2 cut(s) 328, 692
AlwI GGATC 3 cut(s) 453, 483, 496
Aor13HI TCCGGA 1 cut(s) 665
AoxI GGCC 3 cut(s) 255, 359, 456
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 1 cut(s) 541
AspLEI GCGC 1 cut(s) 564
AspS9I GGNCC 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 386
BamHI GGATCC 1 cut(s) 488
BarI GAAGNNNNNNTAC 2 cut(s) 540, 572
BauI CACGAG 1 cut(s) 705
BbvI GCAGC 1 cut(s) 451
BceAI ACGGC 1 cut(s) 575
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 1 cut(s) 440
BlsI GCNGC 1 cut(s) 441
BmcAI AGTACT 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 4 cut(s) 39, 256, 490, 664
BmrI ACTGGG 2 cut(s) 228, 485
BmsI GCATC 3 cut(s) 271, 420, 638
BmuI ACTGGG 2 cut(s) 228, 485
Bpu10I CCTNAGC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 266, 621
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 272
Bse1I ACTGG 3 cut(s) 223, 471, 480
BseAI TCCGGA 1 cut(s) 665
BseDI CCNNGG 2 cut(s) 266, 621
BseGI GGATG 2 cut(s) 229, 262
BseLI CCNNNNNNNGG 2 cut(s) 187, 272
BseMII CTCAG 2 cut(s) 315, 658
BseNI ACTGG 3 cut(s) 223, 471, 480
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 1 cut(s) 451
BseYI CCCAGC 1 cut(s) 725
BshFI GGCC 3 cut(s) 257, 361, 458
BsiSI CCGG 2 cut(s) 443, 666
BslFI GGGAC 3 cut(s) 35, 229, 493
BslI CCNNNNNNNGG 2 cut(s) 187, 272
BsmFI GGGAC 3 cut(s) 35, 229, 493
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 3 cut(s) 257, 361, 458
Bsp1286I GDGCHC 1 cut(s) 386
Bsp13I TCCGGA 1 cut(s) 665
Bsp143I GATC 4 cut(s) 58, 445, 488, 671
Bsp19I CCATGG 1 cut(s) 266
BspANI GGCC 3 cut(s) 257, 361, 458
BspCNI CTCAG 2 cut(s) 316, 657
BspEI TCCGGA 1 cut(s) 665
BspLI GGNNCC 4 cut(s) 39, 256, 490, 664
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 453, 483, 496
BsrI ACTGG 3 cut(s) 223, 471, 480
BssECI CCNNGG 2 cut(s) 266, 621
BssMI GATC 4 cut(s) 58, 445, 488, 671
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 2 cut(s) 266, 621
Bst2BI CACGAG 1 cut(s) 705
Bst4CI ACNGT 2 cut(s) 397, 500
Bst6I CTCTTC 3 cut(s) 3, 225, 641
BstAPI GCANNNNNTGC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 330, 334
BstDEI CTNAG 2 cut(s) 324, 644
BstDSI CCRYGG 1 cut(s) 266
BstENI CCTNNNNNAGG 1 cut(s) 185
BstF5I GGATG 2 cut(s) 229, 262
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 4 cut(s) 61, 448, 491, 674
BstMBI GATC 4 cut(s) 58, 445, 488, 671
BstMWI GCNNNNNNNGC 2 cut(s) 439, 594
BstSLI GKGCMC 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 488
BstYI RGATCY 1 cut(s) 488
BsuRI GGCC 3 cut(s) 257, 361, 458
BtgI CCRYGG 1 cut(s) 266
BtsCI GGATG 2 cut(s) 229, 262
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 330, 334
CfoI GCGC 1 cut(s) 564
Cfr13I GGNCC 1 cut(s) 255
Csp6I GTAC 2 cut(s) 63, 371
CviAII CATG 4 cut(s) 267, 412, 449, 732
CviQI GTAC 2 cut(s) 63, 371
DdeI CTNAG 2 cut(s) 324, 644
DpnI GATC 4 cut(s) 60, 447, 490, 673
DpnII GATC 4 cut(s) 58, 445, 488, 671
Eam1104I CTCTTC 3 cut(s) 3, 225, 641
EarI CTCTTC 3 cut(s) 3, 225, 641
Eco130I CCWWGG 2 cut(s) 266, 621
Eco147I AGGCCT 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 27
EcoNI CCTNNNNNAGG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 266, 621
EcoT22I ATGCAT 1 cut(s) 435
ErhI CCWWGG 2 cut(s) 266, 621
FaeI CATG 4 cut(s) 270, 415, 452, 735
FaqI GGGAC 3 cut(s) 35, 229, 493
FatI CATG 4 cut(s) 266, 411, 448, 731
Fnu4HI GCNGC 1 cut(s) 440
FokI GGATG 2 cut(s) 236, 249
Fsp4HI GCNGC 1 cut(s) 440
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 563
GluI GCNGC 1 cut(s) 440
GsaI CCCAGC 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 3 cut(s) 257, 361, 458
HapII CCGG 2 cut(s) 443, 666
HhaI GCGC 1 cut(s) 564
Hin1II CATG 4 cut(s) 270, 415, 452, 735
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 4 cut(s) 101, 113, 601, 628
HpaII CCGG 2 cut(s) 443, 666
Hpy166II GTNNAC 2 cut(s) 121, 571
Hpy188I TCNGA 1 cut(s) 647
Hpy188III TCNNGA 7 cut(s) 41, 98, 311, 323, 377, 605, 666
Hpy8I GTNNAC 2 cut(s) 121, 571
HpyAV CCTTC 7 cut(s) 22, 79, 233, 266, 293, 317, 542
HpyCH4III ACNGT 2 cut(s) 397, 500
HpyCH4V TGCA 4 cut(s) 433, 533, 588, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 439, 594
HpyF3I CTNAG 2 cut(s) 324, 644
Hsp92II CATG 4 cut(s) 270, 415, 452, 735
HspAI GCGC 1 cut(s) 562
Kpn2I TCCGGA 1 cut(s) 665
Kzo9I GATC 4 cut(s) 58, 445, 488, 671
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 3 cut(s) 271, 420, 638
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 277
MalI GATC 4 cut(s) 60, 447, 490, 673
MboI GATC 4 cut(s) 58, 445, 488, 671
MboII GAAGA 8 cut(s) 20, 85, 94, 96, 242, 537, 628, 729
MflI RGATCY 1 cut(s) 488
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 6 cut(s) 93, 205, 243, 406, 541, 684
MlyI GAGTC 1 cut(s) 610
Mph1103I ATGCAT 1 cut(s) 435
MroI TCCGGA 1 cut(s) 665
MseI TTAA 3 cut(s) 161, 405, 687
MspI CCGG 2 cut(s) 443, 666
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 439, 594
NcoI CCATGG 1 cut(s) 266
NdeII GATC 4 cut(s) 58, 445, 488, 671
NlaIII CATG 4 cut(s) 270, 415, 452, 735
NlaIV GGNNCC 4 cut(s) 39, 256, 490, 664
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 435
PceI AGGCCT 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 3 cut(s) 101, 113, 628
PkrI GCNGC 1 cut(s) 441
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 725
PspN4I GGNNCC 4 cut(s) 39, 256, 490, 664
PspPI GGNCC 1 cut(s) 255
PsuI RGATCY 1 cut(s) 488
RsaI GTAC 2 cut(s) 64, 372
RsaNI GTAC 2 cut(s) 63, 371
SaqAI TTAA 3 cut(s) 161, 405, 687
SatI GCNGC 1 cut(s) 440
Sau3AI GATC 4 cut(s) 58, 445, 488, 671
Sau96I GGNCC 1 cut(s) 255
ScaI AGTACT 1 cut(s) 372
SchI GAGTC 1 cut(s) 610
SduI GDGCHC 1 cut(s) 386
SetI ASST 8 cut(s) 39, 90, 150, 157, 192, 330, 512, 694
SfaNI GCATC 3 cut(s) 271, 420, 638
Sse9I AATT 6 cut(s) 93, 205, 243, 406, 541, 684
SseBI AGGCCT 1 cut(s) 361
SspMI CTAG 1 cut(s) 149
StuI AGGCCT 1 cut(s) 361
StyI CCWWGG 2 cut(s) 266, 621
TaaI ACNGT 2 cut(s) 397, 500
TasI AATT 6 cut(s) 93, 205, 243, 406, 541, 684
TatI WGTACW 1 cut(s) 370
TfiI GAWTC 3 cut(s) 101, 113, 628
Tru1I TTAA 3 cut(s) 161, 405, 687
Tru9I TTAA 3 cut(s) 161, 405, 687
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 2 cut(s) 243, 400
XagI CCTNNNNNAGG 1 cut(s) 185
XapI RAATTY 1 cut(s) 541
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 372
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.