Rh2CG218400

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007022
Physical Location & Seq
Forward (+)
21576582 .. 21579162
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG218400.1

Sequence Viewer

Length: 813 bp
ATGGCAGATGATCAGAATGATGTGAAGGAGGAGGTGGCGCTGGCAGACATTGCCCCTTTTGATCCTAGTAAAAAGAAGAAGAAGAAGAAGGTTGTAATTGAGGATCCAGCTGATGATTCTGTGGACAAGTTGGCTGAGAAAACGGAAAGCCTGGCAGTTTCTGATGGGCAGGACAGTGCATTTTCTGGTCTGAAAAAGAAGAAGAAGAAGCCAATTGAGACCAGTATTTTGAATGAGGAGCCTGGCGATGTACCGGAGGATTTGGATGAGCACCCGGGTGAGGATGAAGAAGGAGAAGGAATTTTACTGGAGGCCTCCAATCCTTGGGAGGGTAGTGATCGTGATTATGAATATGAGGAGCTTCTTGGAAGGGTGTATAACATTCTTAGGGAGAATAATCCGGAGCTAGCTGGAGACAGGCGTAGGACAGTTATGAGGCCTCCACAAGTTCTACGTGAGGGCACAAAGAAAACTGTCTTTGTGAATTTCATGGATCTGTGCAAGACGATGCATAGGCAACCAGAGCATGTCCAGAATTTCTTGTTGGCCGAACTGGGGACAAGTGGATCACTTGATGGACAACAGAGGTTAGTTGTCAAGGGAAGGTTCGCCCCTAAGAATTTTGAAGGGATTCTGCGGCGATATGTCAATGAATATGTGATTTGCCTTGGGTGCAAAAGCCCTGACACTATACTGTCAAAGGAGAATCGCCTGTATTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCAGATCAAACAAGGTTTTGTTGCTCGTGTTGGGCGTAGGAATGCCGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

270

Amino Acids

30.33

Weight (kDa)

5.53

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 139 - 248 1.3e-39 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 1 cut(s) 637
AclWI GGATC 5 cut(s) 56, 98, 111, 501, 574
AcoI YGGCCR 1 cut(s) 546
AcsI RAATTY 4 cut(s) 300, 484, 535, 619
AfaI GTAC 1 cut(s) 252
AfiI CCNNNNNNNGG 4 cut(s) 280, 324, 329, 555
AgsI TTSAA 2 cut(s) 232, 626
AjnI CCWGG 2 cut(s) 150, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AleI CACNNNNGTG 1 cut(s) 276
AluBI AGCT 4 cut(s) 110, 361, 406, 410
AluI AGCT 4 cut(s) 110, 361, 406, 410
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 212, 408
AlwI GGATC 5 cut(s) 56, 98, 111, 501, 574
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 274
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 312, 437, 546
ApoI RAATTY 4 cut(s) 300, 484, 535, 619
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 40
AsuC2I CCSGG 3 cut(s) 275, 276, 805
AsuHPI GGTGA 1 cut(s) 290
AsuNHI GCTAGC 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 464
BamHI GGATCC 1 cut(s) 103
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BauI CACGAG 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 273
BccI CCATC 2 cut(s) 158, 569
BciT130I CCWGG 2 cut(s) 152, 243
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 3 cut(s) 275, 276, 805
BcoDI GTCTC 2 cut(s) 212, 408
BfaI CTAG 2 cut(s) 66, 407
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 639
Bme1390I CCNGG 5 cut(s) 152, 243, 275, 276, 805
BmeT110I CYCGRG 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 105, 240
BmrFI CCNGG 5 cut(s) 152, 243, 275, 276, 805
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 498, 716
BmtI GCTAGC 1 cut(s) 410
BmuI ACTGGG 1 cut(s) 563
BpmI CTGGAG 2 cut(s) 329, 432
BpuMI CCSGG 3 cut(s) 275, 276, 805
BsaAI YACGTR 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 274, 323, 667, 804
BsaWI WCCGGW 2 cut(s) 253, 400
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 324, 329, 555
Bse1I ACTGG 3 cut(s) 222, 312, 558
Bse3DI GCAATG 1 cut(s) 48
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 2 cut(s) 152, 243
BseDI CCNNGG 4 cut(s) 274, 323, 667, 804
BseGI GGATG 2 cut(s) 271, 289
BseLI CCNNNNNNNGG 4 cut(s) 280, 324, 329, 555
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 126, 736, 773
BseNI ACTGG 3 cut(s) 222, 312, 558
BseRI GAGGAG 3 cut(s) 44, 251, 371
BseSI GKGCMC 1 cut(s) 464
BshFI GGCC 3 cut(s) 314, 439, 548
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 4 cut(s) 254, 275, 401, 804
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 4 cut(s) 280, 324, 329, 555
BsmAI GTCTC 2 cut(s) 212, 408
BsmFI GGGAC 1 cut(s) 571
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 3 cut(s) 314, 439, 548
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 273, 464
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
BspACI CCGC 1 cut(s) 637
BspANI GGCC 3 cut(s) 314, 439, 548
BspCNI CTCAG 3 cut(s) 127, 735, 772
BspEI TCCGGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 105, 240
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 5 cut(s) 56, 98, 111, 501, 574
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 3 cut(s) 222, 312, 558
BssECI CCNNGG 4 cut(s) 274, 323, 667, 804
BssMI GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 323, 667
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 152, 243
Bst4CI ACNGT 4 cut(s) 176, 430, 475, 696
BstAPI GCANNNNNTGC 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 42, 408
BstDEI CTNAG 5 cut(s) 135, 386, 615, 722, 759
BstF5I GGATG 2 cut(s) 271, 289
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 8 cut(s) 13, 64, 106, 340, 496, 569, 752, 765
BstMAI GTCTC 2 cut(s) 212, 408
BstMBI GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
BstMWI GCNNNNNNNGC 3 cut(s) 50, 523, 672
BstNI CCWGG 2 cut(s) 152, 243
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 150, 241, 273, 274, 803
BstSLI GKGCMC 1 cut(s) 464
BstX2I RGATCY 2 cut(s) 103, 493
BstYI RGATCY 2 cut(s) 103, 493
BsuRI GGCC 3 cut(s) 314, 439, 548
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 271, 289
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 2 cut(s) 42, 408
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 40
Cfr9I CCCGGG 1 cut(s) 274
Csp6I GTAC 1 cut(s) 251
CviAII CATG 3 cut(s) 490, 527, 810
CviQI GTAC 1 cut(s) 251
DdeI CTNAG 5 cut(s) 135, 386, 615, 722, 759
DpnI GATC 8 cut(s) 12, 63, 105, 339, 495, 568, 751, 764
DpnII GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
EaeI YGGCCR 1 cut(s) 546
Eco130I CCWWGG 2 cut(s) 323, 667
Eco147I AGGCCT 2 cut(s) 314, 439
Eco31I GGTCTC 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 150, 241
EcoT14I CCWWGG 2 cut(s) 323, 667
EcoT22I ATGCAT 1 cut(s) 513
ErhI CCWWGG 2 cut(s) 323, 667
FaeI CATG 3 cut(s) 493, 530, 813
FaqI GGGAC 1 cut(s) 571
FatI CATG 3 cut(s) 489, 526, 809
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 1 cut(s) 638
FokI GGATG 2 cut(s) 278, 296
Fsp4HI GCNGC 1 cut(s) 638
FspBI CTAG 2 cut(s) 66, 407
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 638
GsuI CTGGAG 2 cut(s) 329, 432
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 314, 439, 548
HapII CCGG 4 cut(s) 254, 275, 401, 804
HhaI GCGC 1 cut(s) 40
Hin1II CATG 3 cut(s) 493, 530, 813
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 3 cut(s) 116, 631, 706
HpaII CCGG 4 cut(s) 254, 275, 401, 804
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 5 cut(s) 15, 163, 192, 725, 762
Hpy188III TCNNGA 4 cut(s) 341, 401, 532, 744
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 7 cut(s) 19, 82, 284, 290, 363, 597, 620
HpyCH4III ACNGT 4 cut(s) 176, 430, 475, 696
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 4 cut(s) 179, 501, 511, 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 523, 672
HpyF3I CTNAG 5 cut(s) 135, 386, 615, 722, 759
HpySE526I ACGT 1 cut(s) 454
Hsp92II CATG 3 cut(s) 493, 530, 813
HspAI GCGC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
LmnI GCTCC 3 cut(s) 238, 358, 403
LweI GCATC 2 cut(s) 498, 716
MaeI CTAG 2 cut(s) 66, 407
MaeII ACGT 1 cut(s) 454
MalI GATC 8 cut(s) 12, 63, 105, 339, 495, 568, 751, 764
MboI GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
MboII GAAGA 8 cut(s) 88, 91, 94, 97, 211, 214, 217, 299
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 103, 493
MhlI GDGCHC 2 cut(s) 273, 464
MluCI AATT 6 cut(s) 96, 213, 300, 484, 535, 619
Mph1103I ATGCAT 1 cut(s) 513
MroI TCCGGA 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 630
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 4 cut(s) 254, 275, 401, 804
MspR9I CCNGG 5 cut(s) 152, 243, 275, 276, 805
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 2 cut(s) 152, 243
MwoI GCNNNNNNNGC 3 cut(s) 50, 523, 672
NciI CCSGG 3 cut(s) 275, 276, 805
NdeII GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 3 cut(s) 493, 530, 813
NlaIV GGNNCC 2 cut(s) 105, 240
NsiI ATGCAT 1 cut(s) 513
NspI RCATGY 1 cut(s) 530
OliI CACNNNNGTG 1 cut(s) 276
PceI AGGCCT 2 cut(s) 314, 439
PcsI WCGNNNNNNNCGW 1 cut(s) 790
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 116, 631, 706
PflMI CCANNNNNTGG 1 cut(s) 324
PkrI GCNGC 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 455
Psp6I CCWGG 2 cut(s) 150, 241
PspGI CCWGG 2 cut(s) 150, 241
PspN4I GGNNCC 2 cut(s) 105, 240
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 103, 493
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 252
RsaNI GTAC 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 276
SatI GCNGC 1 cut(s) 638
Sau3AI GATC 8 cut(s) 10, 61, 103, 337, 493, 566, 749, 762
ScrFI CCNGG 5 cut(s) 152, 243, 275, 276, 805
SduI GDGCHC 2 cut(s) 273, 464
SfaNI GCATC 2 cut(s) 498, 716
SmaI CCCGGG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 6 cut(s) 96, 213, 300, 484, 535, 619
SseBI AGGCCT 2 cut(s) 314, 439
SsiI CCGC 1 cut(s) 637
SspMI CTAG 2 cut(s) 66, 407
StuI AGGCCT 2 cut(s) 314, 439
StyD4I CCNGG 5 cut(s) 150, 241, 273, 274, 803
StyI CCWWGG 2 cut(s) 323, 667
TaaI ACNGT 4 cut(s) 176, 430, 475, 696
TaiI ACGT 1 cut(s) 457
TasI AATT 6 cut(s) 96, 213, 300, 484, 535, 619
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 3 cut(s) 116, 631, 706
TscAI CASTG 1 cut(s) 181
TspDTI ATGAA 4 cut(s) 300, 363, 478, 666
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 274
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 324
XapI RAATTY 4 cut(s) 300, 484, 535, 619
XceI RCATGY 1 cut(s) 530
XmaI CCCGGG 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 66, 407
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.