Rh5BG502200

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007022
Physical Location & Seq
Reverse (-)
79858461 .. 79862214
3754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG502200.1

Sequence Viewer

Length: 822 bp
ATGGCTGAAGAGAACAAGAATGCTGTGAAGGAGGAGGTTCCAGATATTGTCCCCTTTGATCGTACTAAAAAGAAGAAAAAGAAGAAGGTGGTAATTCGGGATTCTTCAAGAGATTCTGTGGACAAGTTGGCTGACAAAACAGAAAACCTAGCAGTTTCTGATGGACAGGAAAGTGCATTTTCTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAATCCAGTGTCTTGAATGAAGAGGGTGGTGATACAAGGGAAGATTTGGATGACGGGACTGGGGATGATGAAGAGGGAGAAGGAATTGTTTTAGGGGCCTCGCATCCATGGGAAGGGAGTGATCGTGACTACCACTATGAGGAGCTTCTTGGTAGGGTGTTCAATATCCTTCGGGAGAATAATCCTGAGCTGGCTGGCGACAGGCGTAGGACAACTATGAGGCCTCCACAAGTACTTCGTGAGGGCACAAAGAAAACTGTCTTTGTTAATTTCATGGACTTATGTAAGACGATGCATAGGCAGCCGGATCATGTTATGGCCTTTTTACTGGCTGAACTGGGGACAAGTGGATCCCTTGACGGTCAGCAGAGGTTAGTTGTAAAGGGAAGATTTGCACCCAAGAATTTTGAAGGAATACTACGGCGCTATGTCAACGAATATGTCATTTGCATTGGGTGCAAGAGTCCTGACACTATACTTTCCAAGGAGAATCGCCTCTTTTTTCTCAGATGTGAGAAGGCAGCGCCCATTATTCATAATAGACACATAGAGCAGGCTGGAGTGGTATATAATGTTTATCAAAAGTGGTTATTTTCCAACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

273

Amino Acids

31.07

Weight (kDa)

8.21

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 138 - 244 2.7e-37 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 528, 558, 571
AcsI RAATTY 1 cut(s) 616
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 2 cut(s) 64, 447
AfiI CCNNNNNNNGG 2 cut(s) 326, 352
AgsI TTSAA 6 cut(s) 108, 190, 215, 229, 376, 623
AluBI AGCT 2 cut(s) 358, 403
AluI AGCT 2 cut(s) 358, 403
AlwI GGATC 3 cut(s) 528, 558, 571
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 3 cut(s) 309, 434, 531
ApeKI GCWGC 2 cut(s) 514, 734
ApoI RAATTY 1 cut(s) 616
AspLEI GCGC 2 cut(s) 639, 739
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 461
BamHI GGATCC 1 cut(s) 563
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BbvI GCAGC 2 cut(s) 526, 746
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 650
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 2 cut(s) 640, 740
BisI GCNGC 2 cut(s) 515, 735
BlsI GCNGC 2 cut(s) 516, 736
BmcAI AGTACT 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 3 cut(s) 39, 310, 565
BmrI ACTGGG 2 cut(s) 282, 560
BmsI GCATC 2 cut(s) 325, 495
BmuI ACTGGG 2 cut(s) 282, 560
BpmI CTGGAG 1 cut(s) 792
Bpu10I CCTNAGC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 320, 696
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 352
Bse1I ACTGG 5 cut(s) 209, 219, 277, 546, 555
BseDI CCNNGG 2 cut(s) 320, 696
BseGI GGATG 3 cut(s) 268, 283, 316
BseLI CCNNNNNNNGG 2 cut(s) 326, 352
BseMII CTCAG 2 cut(s) 390, 733
BseNI ACTGG 5 cut(s) 209, 219, 277, 546, 555
BseRI GAGGAG 2 cut(s) 47, 368
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 2 cut(s) 526, 746
BshFI GGCC 3 cut(s) 311, 436, 533
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 3 cut(s) 35, 283, 568
BslI CCNNNNNNNGG 2 cut(s) 326, 352
BsmFI GGGAC 3 cut(s) 35, 283, 568
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 3 cut(s) 311, 436, 533
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 4 cut(s) 58, 334, 520, 563
Bsp19I CCATGG 1 cut(s) 320
BspANI GGCC 3 cut(s) 311, 436, 533
BspCNI CTCAG 2 cut(s) 391, 732
BspLI GGNNCC 3 cut(s) 39, 310, 565
BspPI GGATC 3 cut(s) 528, 558, 571
BsrI ACTGG 5 cut(s) 209, 219, 277, 546, 555
BssECI CCNNGG 2 cut(s) 320, 696
BssMI GATC 4 cut(s) 58, 334, 520, 563
BssT1I CCWWGG 2 cut(s) 320, 696
Bst4CI ACNGT 2 cut(s) 472, 575
Bst6I CTCTTC 3 cut(s) 3, 228, 279
BstAPI GCANNNNNTGC 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 405, 409, 768
BstDEI CTNAG 2 cut(s) 399, 719
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 3 cut(s) 268, 283, 316
BstH2I RGCGCY 2 cut(s) 640, 740
BstHHI GCGC 2 cut(s) 639, 739
BstKTI GATC 4 cut(s) 61, 337, 523, 566
BstMBI GATC 4 cut(s) 58, 334, 520, 563
BstMWI GCNNNNNNNGC 2 cut(s) 514, 669
BstSLI GKGCMC 1 cut(s) 461
BstV1I GCAGC 2 cut(s) 526, 746
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BsuRI GGCC 3 cut(s) 311, 436, 533
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 3 cut(s) 268, 283, 316
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 405, 409, 768
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 2 cut(s) 639, 739
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 63, 446
CviAII CATG 3 cut(s) 321, 487, 524
CviQI GTAC 2 cut(s) 63, 446
DdeI CTNAG 2 cut(s) 399, 719
DpnI GATC 4 cut(s) 60, 336, 522, 565
DpnII GATC 4 cut(s) 58, 334, 520, 563
Eam1104I CTCTTC 3 cut(s) 3, 228, 279
EarI CTCTTC 3 cut(s) 3, 228, 279
Eco130I CCWWGG 2 cut(s) 320, 696
Eco147I AGGCCT 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 320, 696
EcoT22I ATGCAT 1 cut(s) 510
ErhI CCWWGG 2 cut(s) 320, 696
FaeI CATG 3 cut(s) 324, 490, 527
FaqI GGGAC 3 cut(s) 35, 283, 568
FatI CATG 3 cut(s) 320, 486, 523
Fnu4HI GCNGC 2 cut(s) 515, 735
FokI GGATG 3 cut(s) 275, 290, 303
Fsp4HI GCNGC 2 cut(s) 515, 735
FspBI CTAG 1 cut(s) 149
GlaI GCGC 2 cut(s) 638, 738
GluI GCNGC 2 cut(s) 515, 735
GsuI CTGGAG 1 cut(s) 792
HaeII RGCGCY 2 cut(s) 640, 740
HaeIII GGCC 3 cut(s) 311, 436, 533
HapII CCGG 1 cut(s) 518
HhaI GCGC 2 cut(s) 639, 739
Hin1II CATG 3 cut(s) 324, 490, 527
Hin6I GCGC 2 cut(s) 637, 737
HinP1I GCGC 2 cut(s) 637, 737
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 5 cut(s) 101, 113, 215, 676, 703
HpaII CCGG 1 cut(s) 518
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 121, 646
Hpy188I TCNGA 2 cut(s) 160, 722
Hpy188III TCNNGA 9 cut(s) 41, 98, 108, 226, 338, 386, 398, 452, 680
Hpy8I GTNNAC 2 cut(s) 121, 646
HpyAV CCTTC 7 cut(s) 22, 79, 287, 320, 392, 617, 724
HpyCH4III ACNGT 2 cut(s) 472, 575
HpyCH4V TGCA 5 cut(s) 176, 508, 608, 663, 672
HpyF10VI GCNNNNNNNGC 2 cut(s) 514, 669
HpyF3I CTNAG 2 cut(s) 399, 719
Hsp92II CATG 3 cut(s) 324, 490, 527
HspAI GCGC 2 cut(s) 637, 737
Kzo9I GATC 4 cut(s) 58, 334, 520, 563
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 2 cut(s) 526, 746
LweI GCATC 2 cut(s) 325, 495
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 338
MalI GATC 4 cut(s) 60, 336, 522, 565
MboI GATC 4 cut(s) 58, 334, 520, 563
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 4 cut(s) 93, 297, 481, 616
MlyI GAGTC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 1 cut(s) 480
MspI CCGG 1 cut(s) 518
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 514, 669
NcoI CCATGG 1 cut(s) 320
NdeII GATC 4 cut(s) 58, 334, 520, 563
NlaIII CATG 3 cut(s) 324, 490, 527
NlaIV GGNNCC 3 cut(s) 39, 310, 565
NmuCI GTSAC 1 cut(s) 338
NsiI ATGCAT 1 cut(s) 510
PceI AGGCCT 1 cut(s) 436
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 101, 113, 215, 703
PkrI GCNGC 2 cut(s) 516, 736
PleI GAGTC 1 cut(s) 684
PpsI GAGTC 1 cut(s) 684
PspN4I GGNNCC 3 cut(s) 39, 310, 565
PspPI GGNCC 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 563
RsaI GTAC 2 cut(s) 64, 447
RsaNI GTAC 2 cut(s) 63, 446
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 2 cut(s) 515, 735
Sau3AI GATC 4 cut(s) 58, 334, 520, 563
Sau96I GGNCC 1 cut(s) 309
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 1 cut(s) 685
SduI GDGCHC 1 cut(s) 461
SetI ASST 6 cut(s) 39, 90, 150, 360, 405, 587
SfaNI GCATC 2 cut(s) 325, 495
Sse9I AATT 4 cut(s) 93, 297, 481, 616
SseBI AGGCCT 1 cut(s) 436
SspMI CTAG 1 cut(s) 149
StuI AGGCCT 1 cut(s) 436
StyI CCWWGG 2 cut(s) 320, 696
TaaI ACNGT 2 cut(s) 472, 575
TasI AATT 4 cut(s) 93, 297, 481, 616
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 4 cut(s) 101, 113, 215, 703
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 2 cut(s) 514, 734
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 4 cut(s) 246, 297, 475, 737
TspRI CASTG 1 cut(s) 226
XapI RAATTY 1 cut(s) 616
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.