AT5G22130

Mannosyltransferase (PIG-M)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009558
Physical Location & Seq
Reverse (-)
7337158 .. 7340052
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22130.2

Sequence Viewer

Length: 1353 bp
ATGTCTCGTGAATCAGAGAACACTGATGATGAACCATCGAAATGGATGAAGTTCCGTTCACTACTGGTGTTCTCGATGCTTCTGCGTGTGTTTTTGATTGTTTATGGAGAATGGCAAGATGCTCATATGGAGGTCCGGTACACTGATGTTGATTACATTGTATTTTCCGATGCTGCTTCGTTGATGGCATCCGGAGAATCTCCTTACAAGCGAACCACATACCGTTATTCGCCTTTGCTCGCGCTTCTTCTGACCCCTAATTCCTTCTTTCATCGGTCATGGGGAAAGTTTCTCTTCTCAGCTTCTGATTTACTGGTCGGCTGGTTTATCCATAAGATTTTGAAACAACGTAAGGTGCCTGAGAAGATATGTACATATTCTGTAATGGTTTGGCTTTTCAATCCGTTTACATTCACTATTGGAACCAGAGGGAACTGTGAACCCATCGTTTGTGCCATGATATTATGGATCATTCTCTGCCTAATGCAAGGTAATTTGTTACAAGCTGCATTTTGGTACGGGCTTGTTGTGCATTTCAGGGTCTATCCTATTATCTATGCCTTGCCAATCATCCTGGTTCTTGATACCCAGGTATTTAGATCTGGTCAAAAGCCGGCTCTTTTGTATTGGAATACCGGTCAGGCCAAGACACCTGCCAGTAACATGGAGAGAAAAACATTCCTCTTCAATCTCTTGACCACCTTGAAAAGTTTATTTTCCAGGGAGAGAATCATGTTTGCTTTGATATCCGGGGGAGTTTTTCTAGCTTGCAATGCTGTTTCCTTCTATTTTTATGGACAAGAGTTCCTGCATGAGGCTCTCTTATATCACCTTACTCGAACAGATCCAAGGCACAACTTCTCCATCTACTTCTATCACATATATCTTCACTATGAGCGCCAGTTTTCAGCTGTGGAGAAGCTTATCTCGTTTTTACCCCAGTTTACAGTACAGTTTGCTCTAGTCTTCTGCTTCTCCCAGGATCTAGTGTTCTGCATCTTTCTCCAAACCGTTGCATTTGTCACTTTCAATAAGGTGATAACTGCGCAGTACTTTGTGTGGTTTTATTGCCTGCTACCCCTGATTCTACCATGGAGCCATATGAAGCTGAAATGGGAAGGCTTGTTATGCATCATTATGTGGATCGGAGCTCAGACACATTGGCTCTTATGGGGATACATGCTCGAGTTCAAGGGCGTTAATGTCTTTCTACCGCTATGGATCGCGAGTTTGCTGTTTCTGGCTGCAAACACCTTCGTCCTTGTCAGAATCATACAACGCCATCGATTCTCTCCTCTCTTCAGACGGTATGAATCGTCCTCTTCTTCAAACAATGTTACCAAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

450

Amino Acids

52.95

Weight (kDa)

9.13

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-U PF06728 37 - 411 8.1e-12 GPI transamidase subunit PIG-U
Mannosyl_trans PF05007 160 - 445 9.5e-74 Mannosyltransferase (PIG-M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 661
Acc16I TGCGCA 1 cut(s) 1047
Acc36I ACCTGC 1 cut(s) 661
AccB1I GGYRCC 1 cut(s) 355
AccII CGCG 2 cut(s) 242, 1226
AccIII TCCGGA 1 cut(s) 191
AciI CCGC 1 cut(s) 1214
AclWI GGATC 5 cut(s) 476, 839, 990, 1151, 1229
AcuI CTGAAG 1 cut(s) 1285
AfaI GTAC 5 cut(s) 140, 373, 518, 951, 1052
AfiI CCNNNNNNNGG 1 cut(s) 814
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 7 cut(s) 343, 400, 688, 706, 1030, 1192, 1329
AjnI CCWGG 4 cut(s) 573, 588, 719, 978
AloI GAACNNNNNNTCC 2 cut(s) 974, 1006
AluBI AGCT 7 cut(s) 302, 506, 767, 911, 922, 1108, 1151
AluI AGCT 7 cut(s) 302, 506, 767, 911, 922, 1108, 1151
Alw21I GWGCWC 1 cut(s) 1153
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 5 cut(s) 476, 839, 990, 1151, 1229
AlwNI CAGNNNCTG 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 1184
Aor13HI TCCGGA 1 cut(s) 191
AoxI GGCC 1 cut(s) 642
ApeKI GCWGC 3 cut(s) 173, 506, 1244
AsiGI ACCGGT 1 cut(s) 635
AspLEI GCGC 3 cut(s) 244, 900, 1048
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 2 cut(s) 821, 1048
AvaI CYCGRG 1 cut(s) 1184
AvaII GGWCC 1 cut(s) 133
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanI GGYRCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 1153
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 958
Bbv12I GWGCWC 1 cut(s) 1153
BbvI GCAGC 3 cut(s) 160, 493, 1231
BccI CCATC 5 cut(s) 43, 178, 452, 872, 1290
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 4 cut(s) 575, 590, 721, 980
BciVI GTATCC 1 cut(s) 1169
BcnI CCSGG 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 764, 962, 986
BfoI RGCGCY 1 cut(s) 901
BfuAI ACCTGC 1 cut(s) 661
BfuI GTATCC 1 cut(s) 1169
BglII AGATCT 1 cut(s) 599
BisI GCNGC 3 cut(s) 174, 507, 1245
BlsI GCNGC 3 cut(s) 175, 508, 1246
BmcAI AGTACT 1 cut(s) 1052
Bme1390I CCNGG 5 cut(s) 575, 590, 721, 751, 980
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 1184
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 357, 424, 1097
BmrFI CCNGG 5 cut(s) 575, 590, 721, 751, 980
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 6 cut(s) 66, 109, 160, 197, 1005, 1140
BmuI ACTGGG 1 cut(s) 934
BpiI GAAGAC 1 cut(s) 958
BpuMI CCSGG 1 cut(s) 751
Bsa29I ATCGAT 1 cut(s) 1285
BsaJI CCNNGG 6 cut(s) 588, 720, 750, 848, 978, 1091
BsaWI WCCGGW 3 cut(s) 135, 191, 635
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 845, 875
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse118I RCCGGY 2 cut(s) 613, 635
Bse1I ACTGG 5 cut(s) 69, 318, 657, 901, 940
Bse3DI GCAATG 1 cut(s) 778
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 4 cut(s) 575, 590, 721, 980
BseCI ATCGAT 1 cut(s) 1285
BseDI CCNNGG 6 cut(s) 588, 720, 750, 848, 978, 1091
BseGI GGATG 3 cut(s) 51, 188, 570
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 3 cut(s) 312, 351, 1166
BseNI ACTGG 5 cut(s) 69, 318, 657, 901, 940
BseRI GAGGAG 1 cut(s) 1284
BseXI GCAGC 3 cut(s) 160, 493, 1231
Bsh1236I CGCG 2 cut(s) 242, 1226
BshFI GGCC 1 cut(s) 644
BshNI GGYRCC 1 cut(s) 355
BshTI ACCGGT 1 cut(s) 635
BshVI ATCGAT 1 cut(s) 1285
BsiHKAI GWGCWC 1 cut(s) 1153
BsiHKCI CYCGRG 1 cut(s) 1184
BsiSI CCGG 5 cut(s) 136, 192, 614, 636, 750
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 644
BsoBI CYCGRG 1 cut(s) 1184
Bsp1286I GDGCHC 1 cut(s) 1153
Bsp13I TCCGGA 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 371
Bsp143I GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
Bsp19I CCATGG 1 cut(s) 1091
Bsp68I TCGCGA 1 cut(s) 1226
BspACI CCGC 1 cut(s) 1214
BspANI GGCC 1 cut(s) 644
BspCNI CTCAG 3 cut(s) 311, 352, 1165
BspDI ATCGAT 1 cut(s) 1285
BspEI TCCGGA 1 cut(s) 191
BspFNI CGCG 2 cut(s) 242, 1226
BspLI GGNNCC 3 cut(s) 357, 424, 1097
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 5 cut(s) 476, 839, 990, 1151, 1229
BspT107I GGYRCC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 778
BsrFI RCCGGY 2 cut(s) 613, 635
BsrGI TGTACA 1 cut(s) 371
BsrI ACTGG 5 cut(s) 69, 318, 657, 901, 940
BssAI RCCGGY 2 cut(s) 613, 635
BssECI CCNNGG 6 cut(s) 588, 720, 750, 848, 978, 1091
BssMI GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 848, 1091
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 4 cut(s) 575, 590, 721, 980
Bst4CI ACNGT 6 cut(s) 224, 437, 949, 954, 1012, 1308
Bst6I CTCTTC 4 cut(s) 299, 689, 1304, 1327
BstAUI TGTACA 1 cut(s) 371
BstC8I GCNNGC 4 cut(s) 240, 615, 769, 1073
BstDEI CTNAG 3 cut(s) 298, 360, 1152
BstDSI CCRYGG 1 cut(s) 1091
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 3 cut(s) 51, 188, 570
BstFNI CGCG 2 cut(s) 242, 1226
BstH2I RGCGCY 1 cut(s) 901
BstHHI GCGC 3 cut(s) 244, 900, 1048
BstKTI GATC 6 cut(s) 471, 602, 847, 985, 1146, 1224
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
BstMWI GCNNNNNNNGC 3 cut(s) 529, 773, 1128
BstNI CCWGG 4 cut(s) 575, 590, 721, 980
BstNSI RCATGY 1 cut(s) 1183
BstSCI CCNGG 5 cut(s) 573, 588, 719, 749, 978
BstUI CGCG 2 cut(s) 242, 1226
BstV1I GCAGC 3 cut(s) 160, 493, 1231
BstV2I GAAGAC 1 cut(s) 958
BstX2I RGATCY 3 cut(s) 599, 844, 982
BstXI CCANNNNNNTGG 2 cut(s) 42, 664
BstYI RGATCY 3 cut(s) 599, 844, 982
Bsu15I ATCGAT 1 cut(s) 1285
BsuI GTATCC 1 cut(s) 1169
BsuRI GGCC 1 cut(s) 644
BsuTUI ATCGAT 1 cut(s) 1285
BtgI CCRYGG 1 cut(s) 1091
BtsCI GGATG 3 cut(s) 51, 188, 570
BtsIMutI CAGTG 2 cut(s) 21, 141
BtuMI TCGCGA 1 cut(s) 1226
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 4 cut(s) 240, 615, 769, 1073
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 3 cut(s) 244, 900, 1048
Cfr10I RCCGGY 2 cut(s) 613, 635
Cfr13I GGNCC 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 1285
Csp6I GTAC 5 cut(s) 139, 372, 517, 950, 1051
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 7 cut(s) 279, 457, 664, 733, 812, 1092, 1180
CviQI GTAC 5 cut(s) 139, 372, 517, 950, 1051
DdeI CTNAG 3 cut(s) 298, 360, 1152
DpnI GATC 6 cut(s) 470, 601, 846, 984, 1145, 1223
DpnII GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
Eam1104I CTCTTC 4 cut(s) 299, 689, 1304, 1327
EarI CTCTTC 4 cut(s) 299, 689, 1304, 1327
Ecl136II GAGCTC 1 cut(s) 1151
Eco130I CCWWGG 2 cut(s) 848, 1091
Eco24I GRGCYC 1 cut(s) 1153
Eco32I GATATC 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 1151
Eco57I CTGAAG 1 cut(s) 1285
Eco88I CYCGRG 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 1151
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoRII CCWGG 4 cut(s) 573, 588, 719, 978
EcoRV GATATC 1 cut(s) 747
EcoT14I CCWWGG 2 cut(s) 848, 1091
EcoT22I ATGCAT 1 cut(s) 1133
EcoT38I GRGCYC 1 cut(s) 1153
ErhI CCWWGG 2 cut(s) 848, 1091
FaeI CATG 7 cut(s) 282, 460, 667, 736, 815, 1095, 1183
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 7 cut(s) 278, 456, 663, 732, 811, 1091, 1179
FauNDI CATATG 2 cut(s) 126, 1101
Fnu4HI GCNGC 3 cut(s) 174, 507, 1245
FokI GGATG 3 cut(s) 58, 175, 557
FriOI GRGCYC 1 cut(s) 1153
Fsp4HI GCNGC 3 cut(s) 174, 507, 1245
FspBI CTAG 3 cut(s) 764, 962, 986
FspI TGCGCA 1 cut(s) 1047
GlaI GCGC 3 cut(s) 243, 899, 1047
GluI GCNGC 3 cut(s) 174, 507, 1245
HaeII RGCGCY 1 cut(s) 901
HaeIII GGCC 1 cut(s) 644
HapII CCGG 5 cut(s) 136, 192, 614, 636, 750
HhaI GCGC 3 cut(s) 244, 900, 1048
Hin1II CATG 7 cut(s) 282, 460, 667, 736, 815, 1095, 1183
Hin6I GCGC 3 cut(s) 242, 898, 1046
HinP1I GCGC 3 cut(s) 242, 898, 1046
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 7 cut(s) 11, 197, 729, 1084, 1269, 1287, 1313
HpaII CCGG 5 cut(s) 136, 192, 614, 636, 750
HphI GGTGA 2 cut(s) 821, 1048
Hpy166II GTNNAC 5 cut(s) 59, 141, 408, 440, 945
Hpy188I TCNGA 8 cut(s) 16, 169, 252, 307, 1148, 1155, 1268, 1304
Hpy188III TCNNGA 6 cut(s) 8, 73, 192, 581, 694, 1225
Hpy8I GTNNAC 5 cut(s) 59, 141, 408, 440, 945
HpyAV CCTTC 4 cut(s) 274, 793, 1112, 1264
HpyCH4III ACNGT 6 cut(s) 224, 437, 949, 954, 1012, 1308
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 9 cut(s) 487, 509, 532, 771, 811, 996, 1016, 1131, 1247
HpyF10VI GCNNNNNNNGC 3 cut(s) 529, 773, 1128
HpyF3I CTNAG 3 cut(s) 298, 360, 1152
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 7 cut(s) 282, 460, 667, 736, 815, 1095, 1183
HspAI GCGC 3 cut(s) 242, 898, 1046
Kpn2I TCCGGA 1 cut(s) 191
KroI GCCGGC 1 cut(s) 613
KroNI GCCGGC 1 cut(s) 615
Kzo9I GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
LmnI GCTCC 2 cut(s) 1095, 1148
Lsp1109I GCAGC 3 cut(s) 160, 493, 1231
LweI GCATC 6 cut(s) 66, 109, 160, 197, 1005, 1140
MaeI CTAG 3 cut(s) 764, 962, 986
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 4 cut(s) 498, 659, 1021, 1336
MalI GATC 6 cut(s) 470, 601, 846, 984, 1145, 1223
MboI GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
MboII GAAGA 9 cut(s) 239, 286, 376, 676, 878, 958, 1291, 1314, 1317
MflI RGATCY 3 cut(s) 599, 844, 982
MhlI GDGCHC 1 cut(s) 1153
MluCI AATT 2 cut(s) 259, 493
MnlI CCTC 6 cut(s) 124, 422, 692, 808, 1305, 1330
Mph1103I ATGCAT 1 cut(s) 1133
MroI TCCGGA 1 cut(s) 191
MroNI GCCGGC 1 cut(s) 613
MseI TTAA 1 cut(s) 1200
MslI CAYNNNNRTG 2 cut(s) 40, 1136
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 5 cut(s) 136, 192, 614, 636, 750
MspR9I CCNGG 5 cut(s) 575, 590, 721, 751, 980
MvaI CCWGG 4 cut(s) 575, 590, 721, 980
MvnI CGCG 2 cut(s) 242, 1226
MwoI GCNNNNNNNGC 3 cut(s) 529, 773, 1128
NaeI GCCGGC 1 cut(s) 615
NciI CCSGG 1 cut(s) 751
NcoI CCATGG 1 cut(s) 1091
NdeI CATATG 2 cut(s) 126, 1101
NdeII GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
NgoMIV GCCGGC 1 cut(s) 613
NlaIII CATG 7 cut(s) 282, 460, 667, 736, 815, 1095, 1183
NlaIV GGNNCC 3 cut(s) 357, 424, 1097
NmuCI GTSAC 1 cut(s) 1021
NruI TCGCGA 1 cut(s) 1226
NsbI TGCGCA 1 cut(s) 1047
NsiI ATGCAT 1 cut(s) 1133
NspI RCATGY 1 cut(s) 1183
PaeR7I CTCGAG 1 cut(s) 1184
PaqCI CACCTGC 1 cut(s) 661
PdiI GCCGGC 1 cut(s) 615
PfeI GAWTC 7 cut(s) 11, 197, 729, 1084, 1269, 1287, 1313
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 3 cut(s) 175, 508, 1246
Psp124BI GAGCTC 1 cut(s) 1153
Psp6I CCWGG 4 cut(s) 573, 588, 719, 978
PspGI CCWGG 4 cut(s) 573, 588, 719, 978
PspN4I GGNNCC 3 cut(s) 357, 424, 1097
PspPI GGNCC 1 cut(s) 133
PspXI VCTCGAGB 1 cut(s) 1184
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 3 cut(s) 599, 844, 982
PvuII CAGCTG 1 cut(s) 911
RruI TCGCGA 1 cut(s) 1226
RsaI GTAC 5 cut(s) 140, 373, 518, 951, 1052
RsaNI GTAC 5 cut(s) 139, 372, 517, 950, 1051
RseI CAYNNNNRTG 2 cut(s) 40, 1136
SacI GAGCTC 1 cut(s) 1153
SaqAI TTAA 1 cut(s) 1200
SatI GCNGC 3 cut(s) 174, 507, 1245
Sau3AI GATC 6 cut(s) 468, 599, 844, 982, 1143, 1221
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 1052
ScrFI CCNGG 5 cut(s) 575, 590, 721, 751, 980
SduI GDGCHC 1 cut(s) 1153
SfaNI GCATC 6 cut(s) 66, 109, 160, 197, 1005, 1140
Sfr274I CTCGAG 1 cut(s) 1184
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 1184
SmiMI CAYNNNNRTG 2 cut(s) 40, 1136
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
Sse9I AATT 2 cut(s) 259, 493
SsiI CCGC 1 cut(s) 1214
SspMI CTAG 3 cut(s) 764, 962, 986
SstI GAGCTC 1 cut(s) 1153
StyD4I CCNGG 5 cut(s) 573, 588, 719, 749, 978
StyI CCWWGG 2 cut(s) 848, 1091
TaaI ACNGT 6 cut(s) 224, 437, 949, 954, 1012, 1308
TaiI ACGT 1 cut(s) 352
TaqI TCGA 5 cut(s) 38, 74, 838, 1185, 1285
TaqII GACCGA 1 cut(s) 264
TasI AATT 2 cut(s) 259, 493
TatI WGTACW 3 cut(s) 371, 949, 1050
TfiI GAWTC 7 cut(s) 11, 197, 729, 1084, 1269, 1287, 1313
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 2 cut(s) 28, 148
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 3 cut(s) 173, 506, 1244
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 5 cut(s) 45, 62, 260, 1118, 1326
TspGWI ACGGA 2 cut(s) 44, 393
TspRI CASTG 2 cut(s) 28, 148
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 812
XceI RCATGY 1 cut(s) 1183
XhoI CTCGAG 1 cut(s) 1184
XspI CTAG 3 cut(s) 764, 962, 986
ZrmI AGTACT 1 cut(s) 1052
Zsp2I ATGCAT 1 cut(s) 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.