Rorug06G0123600

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009558
Physical Location & Seq
Forward (+)
17153237 .. 17154097
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0123600.1

Sequence Viewer

Length: 834 bp
ATGTATCTCTACCTTCTCCCAAACATTTCTTCACCCCTCCTCCTCATAACCCTGACCATATTCTTATTTCATGTTCCAAACTCTCTGAGCTTTGATGATGAGCGATACCTCAACTGCAGCCAATCGTTTGAATGCTCCAATCTTGAGAGCATCGGTTACCCTTTCTGGGGAGCAGACCGGCCTGAATACTGTGGCTTCCCGGACTTCAAGTTGAACTGCAGCGGCGATGCCCCATCCATCTCAATCCAGGAACAAGAGTTTCGAGTCCTTTCTATCAATCAGAGTGAAAGCATTCTCACAGTCGCTAGAGTCGATTTCTGGAACAACGTTTGTCCGGTGTCCCTTATCAATACCACTGTCATAGATGATTCATCATCCCGTGTTCTTGAATATGCTTCAGATGTTCAACAACTCCTGCTCTTCTACGCATGTCCCCCACAAAACATTCCTCTCCCGCCTCAGCTTACCAGCCAATTCAACTGCACCTTAAACTCTACTACTGATTCCATCAACTATTATGTGACAGAAGATCTTAAAAATTTCAGGCTCTCCAAAGAGCCAAGCACCTTTGGAACGTGTGGCACAACTGTCACCGTCCGACTTTCTCAATCGGCAGCTTCAAATTTGGCTCTGAATACGTCCAAGGAAAATCTAGGAGCTGCGCTGGATTCTGGGTTTGGGCTGAAGTGGGATGCAAGTAATAACTTGTGTGAACAATGTCAAGGCTCTGGGGGTCAGTGCGGATATAACACATCTTCACGTGAATTCAGCTGCTACTGCAAGGATGGACCTAATCCTTCCAACTGTGGAGGTGTGCGCCAACTAAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

277

Amino Acids

30.62

Weight (kDa)

4.42

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 103 2.8e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 160 - 263 1e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 222, 455, 741
AclI AACGTT 1 cut(s) 327
AcsI RAATTY 3 cut(s) 538, 622, 764
AcuI CTGAAG 2 cut(s) 381, 704
AcvI CACGTG 1 cut(s) 761
AfiI CCNNNNNNNGG 2 cut(s) 166, 167
AflIII ACRYGT 1 cut(s) 575
AgsI TTSAA 7 cut(s) 131, 208, 214, 389, 407, 478, 621
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 5 cut(s) 90, 463, 617, 659, 771
AluI AGCT 5 cut(s) 90, 463, 617, 659, 771
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 5 cut(s) 117, 219, 614, 659, 771
ApoI RAATTY 3 cut(s) 538, 622, 764
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 2 cut(s) 664, 819
AspS9I GGNCC 1 cut(s) 788
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 24, 583
AvaII GGWCC 1 cut(s) 788
BbrPI CACGTG 1 cut(s) 761
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 5 cut(s) 129, 231, 626, 646, 758
BccI CCATC 4 cut(s) 241, 245, 515, 779
BciT130I CCWGG 1 cut(s) 248
BcnI CCSGG 1 cut(s) 200
BfaI CTAG 2 cut(s) 306, 653
BfmI CTRYAG 2 cut(s) 115, 217
BglII AGATCT 1 cut(s) 529
BisI GCNGC 6 cut(s) 118, 220, 223, 615, 660, 772
BlsI GCNGC 6 cut(s) 119, 221, 224, 616, 661, 773
Bme1390I CCNGG 2 cut(s) 200, 248
Bme18I GGWCC 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 788
BmrFI CCNGG 2 cut(s) 200, 248
BmsI GCATC 3 cut(s) 159, 217, 682
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 761
BsaJI CCNNGG 1 cut(s) 642
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 167
Bse118I RCCGGY 1 cut(s) 177
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 4 cut(s) 233, 374, 697, 790
BseLI CCNNNNNNNGG 2 cut(s) 166, 167
BseMII CTCAG 2 cut(s) 77, 473
BseRI GAGGAG 2 cut(s) 29, 32
BseXI GCAGC 5 cut(s) 129, 231, 626, 646, 758
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 1 cut(s) 181
BsiSI CCGG 3 cut(s) 178, 200, 335
BslFI GGGAC 2 cut(s) 325, 417
BslI CCNNNNNNNGG 2 cut(s) 166, 167
BsmFI GGGAC 2 cut(s) 325, 417
BsmI GAATGC 2 cut(s) 137, 291
BsnI GGCC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 529
BspACI CCGC 3 cut(s) 222, 455, 741
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 2 cut(s) 78, 472
BspMAI CTGCAG 2 cut(s) 119, 221
BspQI GCTCTTC 1 cut(s) 425
BsrFI RCCGGY 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 6 cut(s) 191, 301, 358, 589, 595, 806
Bst6I CTCTTC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 761
BstDEI CTNAG 2 cut(s) 86, 459
BstEII GGTNACC 1 cut(s) 155
BstF5I GGATG 4 cut(s) 233, 374, 697, 790
BstHHI GCGC 2 cut(s) 664, 819
BstKTI GATC 1 cut(s) 532
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 1 cut(s) 777
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 432
BstPI GGTNACC 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 198, 246
BstSFI CTRYAG 2 cut(s) 115, 217
BstV1I GCAGC 5 cut(s) 129, 231, 626, 646, 758
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 233, 374, 697, 790
BtsIMutI CAGTG 2 cut(s) 354, 743
CfoI GCGC 2 cut(s) 664, 819
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 788
CviAII CATG 2 cut(s) 71, 429
DdeI CTNAG 2 cut(s) 86, 459
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Eco130I CCWWGG 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 788
Eco57I CTGAAG 2 cut(s) 381, 704
Eco72I CACGTG 1 cut(s) 761
Eco91I GGTNACC 1 cut(s) 155
EcoO65I GGTNACC 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 2 cut(s) 74, 432
FaiI YATR 8 cut(s) 47, 59, 72, 362, 393, 430, 519, 747
FaqI GGGAC 2 cut(s) 325, 417
FatI CATG 2 cut(s) 70, 428
FauI CCCGC 1 cut(s) 462
Fnu4HI GCNGC 6 cut(s) 118, 220, 223, 615, 660, 772
FokI GGATG 4 cut(s) 220, 361, 704, 797
Fsp4HI GCNGC 6 cut(s) 118, 220, 223, 615, 660, 772
FspBI CTAG 2 cut(s) 306, 653
GlaI GCGC 2 cut(s) 663, 818
GluI GCNGC 6 cut(s) 118, 220, 223, 615, 660, 772
HaeIII GGCC 1 cut(s) 181
HapII CCGG 3 cut(s) 178, 200, 335
HhaI GCGC 2 cut(s) 664, 819
Hin1II CATG 2 cut(s) 74, 432
Hin6I GCGC 2 cut(s) 662, 817
HinP1I GCGC 2 cut(s) 662, 817
HinfI GANTC 5 cut(s) 264, 309, 368, 503, 668
HpaII CCGG 3 cut(s) 178, 200, 335
HphI GGTGA 2 cut(s) 24, 583
Hpy166II GTNNAC 1 cut(s) 713
Hpy188I TCNGA 5 cut(s) 87, 282, 400, 599, 633
Hpy188III TCNNGA 3 cut(s) 143, 319, 386
Hpy8I GTNNAC 1 cut(s) 713
HpyAV CCTTC 2 cut(s) 23, 807
HpyCH4III ACNGT 6 cut(s) 191, 301, 358, 589, 595, 806
HpyCH4IV ACGT 4 cut(s) 327, 575, 638, 760
HpyCH4V TGCA 5 cut(s) 117, 219, 483, 695, 780
HpyF10VI GCNNNNNNNGC 1 cut(s) 777
HpyF3I CTNAG 2 cut(s) 86, 459
HpySE526I ACGT 4 cut(s) 327, 575, 638, 760
Hsp92II CATG 2 cut(s) 74, 432
HspAI GCGC 2 cut(s) 662, 817
Kzo9I GATC 1 cut(s) 529
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 3 cut(s) 140, 170, 656
Lsp1109I GCAGC 5 cut(s) 129, 231, 626, 646, 758
LweI GCATC 3 cut(s) 159, 217, 682
MaeI CTAG 2 cut(s) 306, 653
MaeII ACGT 4 cut(s) 327, 575, 638, 760
MaeIII GTNAC 3 cut(s) 155, 520, 589
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 4 cut(s) 21, 412, 539, 747
MflI RGATCY 1 cut(s) 529
MluCI AATT 5 cut(s) 473, 538, 622, 764, 826
MlyI GAGTC 2 cut(s) 273, 318
MmeI TCCRAC 2 cut(s) 622, 825
MnlI CCTC 7 cut(s) 47, 50, 53, 119, 459, 468, 803
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 2 cut(s) 488, 534
MspA1I CMGCKG 2 cut(s) 222, 771
MspI CCGG 3 cut(s) 178, 200, 335
MspR9I CCNGG 2 cut(s) 200, 248
Mva1269I GAATGC 2 cut(s) 137, 291
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 777
NciI CCSGG 1 cut(s) 200
NdeII GATC 1 cut(s) 529
NlaIII CATG 2 cut(s) 74, 432
NmuCI GTSAC 2 cut(s) 520, 589
NspI RCATGY 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 2 cut(s) 137, 291
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 368, 503, 668
PfoI TCCNGGA 2 cut(s) 198, 246
PkrI GCNGC 6 cut(s) 119, 221, 224, 616, 661, 773
PleI GAGTC 2 cut(s) 272, 317
PmaCI CACGTG 1 cut(s) 761
PmlI CACGTG 1 cut(s) 761
PpsI GAGTC 2 cut(s) 272, 317
Ppu21I YACGTR 1 cut(s) 761
Psp1406I AACGTT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 246
PspCI CACGTG 1 cut(s) 761
PspEI GGTNACC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 246
PspPI GGNCC 1 cut(s) 788
PstI CTGCAG 2 cut(s) 119, 221
PsuI RGATCY 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 2 cut(s) 488, 534
SatI GCNGC 6 cut(s) 118, 220, 223, 615, 660, 772
Sau3AI GATC 1 cut(s) 529
Sau96I GGNCC 1 cut(s) 788
SchI GAGTC 2 cut(s) 273, 318
ScrFI CCNGG 2 cut(s) 200, 248
SfaNI GCATC 3 cut(s) 159, 217, 682
SfcI CTRYAG 2 cut(s) 115, 217
SinI GGWCC 1 cut(s) 788
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 5 cut(s) 473, 538, 622, 764, 826
SsiI CCGC 3 cut(s) 222, 455, 741
SspMI CTAG 2 cut(s) 306, 653
StyD4I CCNGG 2 cut(s) 198, 246
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 6 cut(s) 191, 301, 358, 589, 595, 806
TaiI ACGT 4 cut(s) 330, 578, 641, 763
TaqI TCGA 2 cut(s) 262, 312
TasI AATT 5 cut(s) 473, 538, 622, 764, 826
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 3 cut(s) 368, 503, 668
Tru1I TTAA 2 cut(s) 488, 534
Tru9I TTAA 2 cut(s) 488, 534
TscAI CASTG 2 cut(s) 361, 743
TseFI GTSAC 2 cut(s) 520, 589
TseI GCWGC 5 cut(s) 117, 219, 614, 659, 771
Tsp45I GTSAC 2 cut(s) 520, 589
TspDTI ATGAA 2 cut(s) 59, 360
TspRI CASTG 2 cut(s) 361, 743
VpaK11BI GGWCC 1 cut(s) 788
XapI RAATTY 3 cut(s) 538, 622, 764
XceI RCATGY 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 2 cut(s) 306, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.