Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0009558

Physical Location & Seq

Genomic Coordinates Forward (+)

14725163 .. 14727511

Gene Structure 2349 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g11190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGGCGCCCATACAATTCCGTGCGCTCCTCATCGTCTCGGCGGTGCTGAGGGTGGTTTTAATCCTGTACGGAGAGTGGCAGGATGCCCACATGGAGGTGAGGTACACAGACGTCGATTACATAGTCTTCTCGGACGCAGCTTCTCTAATGGCGTCTGGTAAATCCCCATTTCAGAGATCCACTTACAGATACTCTCCTCTGCTCGCCCTTCTGCTCCTACCCAATTCCATTCTCCATCGCTCTTGGGGCAAATTCATCTTCTCGGCTGCAGATTTGCTTGTGGGTTTGTTTATAAGGATCATTCTGAAGCTTCGCGGGGTGTCGGAGAAATTGAGCATGATTTCTGTGATTGTTTGGCTCTTTAATCCGTTTACTTTTACGATTGGGACTCGAGGGAATTGTGAGCCAATTGTTTGTGCTTTGGTTCTTTGGGTCTTCGTATGTCTTATGAACGGACGTGTGTTACAAGCTGCATTTTGGTATGGGCTTGTTGTCCACTTCAGAATCTACCCCATTATCTATGCACTCCCTATCCTTGTGATTATGGATCCACATGTGTTTCGACCTGGTCAGAAGCCTAGACTTCAATATTGGAATCGTCGGCAAGATTCACCTCAAAGCAGTTCTGTTACAAGACTCACTCAGCTTCTTTGGCCATGGTTTCTTTTGAAAAGTGCATTAACACGAGAAAGATTACTTTTCTCCTTTATATCTGCGGCCGTATTCTTCCTTTTCACTGGTTTATTCTATCATCTGTATGGGTGGGATTTCCTGTATGAAGCACTGTTGTATCATCTTACCCGTACTGATCCAAGGCACAATTTTTCCATTTACTTTTATCACATCTACCTTCAGTACCACCGTAAACTTTCGATATTGGAGAAACTCGTCTCATTTTTGCCTCAGTTGATAGTCCAGGTTGTTCTTGTTTTTAGTTTCGCCAAAGACCTTCCATTCTGCTGCTTAGTACAGACAGTGGCTTTCGTGGCTTTCAACAAGGTCATGACTGCACAATACTTTGTGTGGTTCTTTTGTCTCTTGCCGCTGGTTCTCGCTTGGAGCAACATGAAGCTTAAATGGAAAGGCCTATCGTGCATCGCATTGTGGGTAGGCGGTCAACTTCATTGGTTGTTTTGGGGTTATATGCTTGAGTTCAAAGGGCAAAATGTCTTTCTTCAACTCTGGATGGCAAGCATCATATTCTTAGCTGCGAACACGTACGTCTTAGTTTCGCTCATCCGCGACCACAGATTTTCTCAAGTTTTCGCACGGTTGGAGAAAACCTCCAAGGACTCGAAGAAACTTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Mannosyl_trans

Protein Analysis

437

Amino Acids

51.03

Weight (kDa)

9.79

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIG-U	PF06728	26 - 357	5e-10	GPI transamidase subunit PIG-U
Mannosyl_trans	PF05007	128 - 413	8.7e-73	Mannosyltransferase (PIG-M)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009558)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22130 AT5G22130 AT5G22130
fragaria_vesca	FvH4_2g15820 FvH4_2g15820
malus_domestica	MD05G1127200.v1.1 MD10G1130600.v1.1
prunus_persica	Prupe.8G170900_v2.0.a1
pyrus_communis	pycom05g12300 pycom10g11190
rosa_chinensis	RchiOBHm_Chr6g0279191
rosa_laevigata	RLG00000013156
rosa_multiflora	Rmu_sc0014203.1_g000010 Rmu_sc0014536.1_g000005
rosa_roxburghii	Rroxscaffold_7G00189040
rosa_rugosa	Rorug06G0123600 Rorug06G0123700
rosa_samantha	Rh6AG231900 Rh6BG236800 Rh6CG238700 Rh6DG230300
rosa_wichuraiana	Rw6G020300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	293
AatII	GACGTC	1 cut(s)	114
AccB1I	GGYRCC	1 cut(s)	4
AccII	CGCG	2 cut(s)	315, 1242
AciI	CCGC	6 cut(s)	41, 315, 716, 1043, 1113, 1240
AclWI	GGATC	5 cut(s)	171, 305, 542, 555, 803
AcoI	YGGCCR	2 cut(s)	653, 717
AcsI	RAATTY	1 cut(s)	251
AcuI	CTGAAG	3 cut(s)	326, 484, 836
AcyI	GRCGYC	3 cut(s)	5, 111, 152
AfaI	GTAC	6 cut(s)	68, 104, 805, 857, 969, 1220
AfiI	CCNNNNNNNGG	1 cut(s)	94
AflII	CTTAAG	1 cut(s)	1304
AflIII	ACRYGT	3 cut(s)	457, 553, 1215
AgsI	TTSAA	5 cut(s)	587, 670, 994, 1156, 1178
AjiI	CACGTC	1 cut(s)	458
AjnI	CCWGG	2 cut(s)	565, 915
AluBI	AGCT	6 cut(s)	140, 310, 470, 646, 1072, 1208
AluI	AGCT	6 cut(s)	140, 310, 470, 646, 1072, 1208
Alw26I	GTCTC	3 cut(s)	40, 895, 1040
AlwI	GGATC	5 cut(s)	171, 305, 542, 555, 803
Ama87I	CYCGRG	1 cut(s)	390
AoxI	GGCC	3 cut(s)	653, 717, 1084
ApeKI	GCWGC	5 cut(s)	137, 266, 470, 960, 1208
ApoI	RAATTY	1 cut(s)	251
AspLEI	GCGC	2 cut(s)	7, 25
AsuHPI	GGTGA	2 cut(s)	109, 603
AvaI	CYCGRG	1 cut(s)	390
BaeI	ACNNNNGTAYC	2 cut(s)	773, 806
BalI	TGGCCA	1 cut(s)	655
BamHI	GGATCC	1 cut(s)	547
BanI	GGYRCC	1 cut(s)	4
BauI	CACGAG	1 cut(s)	684
BbsI	GAAGAC	2 cut(s)	118, 427
BbvCI	CCTCAGC	1 cut(s)	47
BbvI	GCAGC	5 cut(s)	149, 253, 457, 947, 1195
BccI	CCATC	2 cut(s)	243, 1180
BceAI	ACGGC	1 cut(s)	704
BciT130I	CCWGG	2 cut(s)	567, 917
BcoDI	GTCTC	3 cut(s)	40, 895, 1040
BfaI	CTAG	1 cut(s)	579
BfmI	CTRYAG	1 cut(s)	267
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	1304
BisI	GCNGC	7 cut(s)	138, 267, 471, 717, 961, 1043, 1209
BlsI	GCNGC	7 cut(s)	139, 268, 472, 718, 962, 1044, 1210
Bme1390I	CCNGG	2 cut(s)	567, 917
BmeT110I	CYCGRG	1 cut(s)	390
BmgBI	CACGTC	1 cut(s)	458
BmiI	GGNNCC	2 cut(s)	6, 549
BmrFI	CCNGG	2 cut(s)	567, 917
BmsI	GCATC	3 cut(s)	73, 1104, 1203
BpiI	GAAGAC	2 cut(s)	118, 427
BplI	GAGNNNNNCTC	2 cut(s)	1268, 1300
Bpu10I	CCTNAGC	1 cut(s)	47
BpuEI	CTTGAG	2 cut(s)	1169, 1242
BsaAI	YACGTR	1 cut(s)	1218
BsaHI	GRCGYC	3 cut(s)	5, 111, 152
BsaJI	CCNNGG	3 cut(s)	656, 812, 1287
BsaXI	ACNNNNNCTCC	2 cut(s)	86, 116
Bsc4I	CCNNNNNNNGG	1 cut(s)	94
Bse1I	ACTGG	1 cut(s)	742
BseBI	CCWGG	2 cut(s)	567, 917
BseDI	CCNNGG	3 cut(s)	656, 812, 1287
BseGI	GGATG	3 cut(s)	88, 1191, 1236
BseLI	CCNNNNNNNGG	1 cut(s)	94
BseMII	CTCAG	3 cut(s)	38, 656, 917
BseNI	ACTGG	1 cut(s)	742
BseRI	GAGGAG	2 cut(s)	17, 186
BseX3I	CGGCCG	1 cut(s)	717
BseXI	GCAGC	5 cut(s)	149, 253, 457, 947, 1195
BsgI	GTGCAG	1 cut(s)	993
Bsh1236I	CGCG	2 cut(s)	315, 1242
Bsh1285I	CGRYCG	1 cut(s)	720
BshFI	GGCC	3 cut(s)	655, 719, 1086
BshNI	GGYRCC	1 cut(s)	4
BsiEI	CGRYCG	1 cut(s)	720
BsiHKCI	CYCGRG	1 cut(s)	390
BsiWI	CGTACG	1 cut(s)	1218
BslFI	GGGAC	1 cut(s)	400
BslI	CCNNNNNNNGG	1 cut(s)	94
BsmAI	GTCTC	3 cut(s)	40, 895, 1040
BsmBI	CGTCTC	2 cut(s)	40, 895
BsmFI	GGGAC	1 cut(s)	400
BsnI	GGCC	3 cut(s)	655, 719, 1086
BsoBI	CYCGRG	1 cut(s)	390
Bsp143I	GATC	4 cut(s)	176, 297, 547, 808
Bsp19I	CCATGG	1 cut(s)	656
BspACI	CCGC	6 cut(s)	41, 315, 716, 1043, 1113, 1240
BspANI	GGCC	3 cut(s)	655, 719, 1086
BspCNI	CTCAG	3 cut(s)	39, 655, 916
BspFNI	CGCG	2 cut(s)	315, 1242
BspHI	TCATGA	1 cut(s)	1002
BspLI	GGNNCC	2 cut(s)	6, 549
BspMAI	CTGCAG	1 cut(s)	271
BspPI	GGATC	5 cut(s)	171, 305, 542, 555, 803
BspT107I	GGYRCC	1 cut(s)	4
BspTI	CTTAAG	1 cut(s)	1304
BsrI	ACTGG	1 cut(s)	742
BssECI	CCNNGG	3 cut(s)	656, 812, 1287
BssMI	GATC	4 cut(s)	176, 297, 547, 808
BssNI	GRCGYC	3 cut(s)	5, 111, 152
BssSI	CACGAG	1 cut(s)	684
BssT1I	CCWWGG	3 cut(s)	656, 812, 1287
Bst2BI	CACGAG	1 cut(s)	684
Bst2UI	CCWGG	2 cut(s)	567, 917
Bst4CI	ACNGT	4 cut(s)	786, 863, 976, 1272
BstACI	GRCGYC	3 cut(s)	5, 111, 152
BstAFI	CTTAAG	1 cut(s)	1304
BstBAI	YACGTR	1 cut(s)	1218
BstC8I	GCNNGC	2 cut(s)	204, 1192
BstDEI	CTNAG	6 cut(s)	47, 642, 903, 964, 1204, 1225
BstDSI	CCRYGG	1 cut(s)	656
BstF5I	GGATG	3 cut(s)	88, 1191, 1236
BstFNI	CGCG	2 cut(s)	315, 1242
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 25
BstKTI	GATC	4 cut(s)	179, 300, 550, 811
BstMAI	GTCTC	3 cut(s)	40, 895, 1040
BstMBI	GATC	4 cut(s)	176, 297, 547, 808
BstMCI	CGRYCG	1 cut(s)	720
BstMWI	GCNNNNNNNGC	4 cut(s)	246, 652, 986, 1092
BstNI	CCWGG	2 cut(s)	567, 917
BstNSI	RCATGY	1 cut(s)	557
BstSCI	CCNGG	2 cut(s)	565, 915
BstSFI	CTRYAG	1 cut(s)	267
BstUI	CGCG	2 cut(s)	315, 1242
BstV1I	GCAGC	5 cut(s)	149, 253, 457, 947, 1195
BstV2I	GAAGAC	2 cut(s)	118, 427
BstX2I	RGATCY	2 cut(s)	176, 547
BstYI	RGATCY	2 cut(s)	176, 547
BstZI	CGGCCG	1 cut(s)	717
BsuRI	GGCC	3 cut(s)	655, 719, 1086
BtgI	CCRYGG	1 cut(s)	656
BtgZI	GCGATG	2 cut(s)	221, 1081
BtrI	CACGTC	1 cut(s)	458
BtsCI	GGATG	3 cut(s)	88, 1191, 1236
BtsIMutI	CAGTG	3 cut(s)	735, 782, 981
Cac8I	GCNNGC	2 cut(s)	204, 1192
CciI	TCATGA	1 cut(s)	1002
CfoI	GCGC	2 cut(s)	7, 25
CseI	GACGC	2 cut(s)	141, 143
CsiI	ACCWGGT	1 cut(s)	565
Csp6I	GTAC	6 cut(s)	67, 103, 804, 856, 968, 1219
CviAII	CATG	6 cut(s)	91, 337, 554, 657, 1003, 1066
CviQI	GTAC	6 cut(s)	67, 103, 804, 856, 968, 1219
DdeI	CTNAG	6 cut(s)	47, 642, 903, 964, 1204, 1225
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	4 cut(s)	178, 299, 549, 810
DpnII	GATC	4 cut(s)	176, 297, 547, 808
EaeI	YGGCCR	2 cut(s)	653, 717
EagI	CGGCCG	1 cut(s)	717
EclXI	CGGCCG	1 cut(s)	717
Eco130I	CCWWGG	3 cut(s)	656, 812, 1287
Eco147I	AGGCCT	1 cut(s)	1086
Eco52I	CGGCCG	1 cut(s)	717
Eco57I	CTGAAG	3 cut(s)	326, 484, 836
Eco88I	CYCGRG	1 cut(s)	390
EcoRII	CCWGG	2 cut(s)	565, 915
EcoT14I	CCWWGG	3 cut(s)	656, 812, 1287
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	656, 812, 1287
Esp3I	CGTCTC	2 cut(s)	40, 895
FaeI	CATG	6 cut(s)	94, 340, 557, 660, 1006, 1069
FaqI	GGGAC	1 cut(s)	400
FatI	CATG	6 cut(s)	90, 336, 553, 656, 1002, 1065
FauI	CCCGC	1 cut(s)	308
Fnu4HI	GCNGC	7 cut(s)	138, 267, 471, 717, 961, 1043, 1209
FokI	GGATG	3 cut(s)	95, 1198, 1223
Fsp4HI	GCNGC	7 cut(s)	138, 267, 471, 717, 961, 1043, 1209
FspBI	CTAG	1 cut(s)	579
GlaI	GCGC	2 cut(s)	6, 24
GluI	GCNGC	7 cut(s)	138, 267, 471, 717, 961, 1043, 1209
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	3 cut(s)	655, 719, 1086
HgaI	GACGC	2 cut(s)	141, 143
HhaI	GCGC	2 cut(s)	7, 25
Hin1I	GRCGYC	3 cut(s)	5, 111, 152
Hin1II	CATG	6 cut(s)	94, 340, 557, 660, 1006, 1069
Hin6I	GCGC	2 cut(s)	5, 23
HinP1I	GCGC	2 cut(s)	5, 23
HincII	GTYRAC	1 cut(s)	1118
HindII	GTYRAC	1 cut(s)	1118
HindIII	AAGCTT	2 cut(s)	308, 1070
HinfI	GANTC	6 cut(s)	388, 504, 595, 608, 636, 1292
HphI	GGTGA	2 cut(s)	109, 603
Hpy166II	GTNNAC	5 cut(s)	105, 372, 496, 866, 1118
Hpy188I	TCNGA	6 cut(s)	133, 174, 306, 325, 503, 573
Hpy188III	TCNNGA	2 cut(s)	1003, 1183
Hpy8I	GTNNAC	5 cut(s)	105, 372, 496, 866, 1118
Hpy99I	CGWCG	2 cut(s)	116, 603
HpyAV	CCTTC	3 cut(s)	218, 860, 959
HpyCH4III	ACNGT	4 cut(s)	786, 863, 976, 1272
HpyCH4IV	ACGT	4 cut(s)	111, 457, 1217, 1221
HpyCH4V	TGCA	6 cut(s)	269, 473, 524, 677, 1010, 1095
HpyF10VI	GCNNNNNNNGC	4 cut(s)	246, 652, 986, 1092
HpyF3I	CTNAG	6 cut(s)	47, 642, 903, 964, 1204, 1225
HpySE526I	ACGT	4 cut(s)	111, 457, 1217, 1221
Hsp92I	GRCGYC	3 cut(s)	5, 111, 152
Hsp92II	CATG	6 cut(s)	94, 340, 557, 660, 1006, 1069
HspAI	GCGC	2 cut(s)	5, 23
KasI	GGCGCC	1 cut(s)	4
Kzo9I	GATC	4 cut(s)	176, 297, 547, 808
LmnI	GCTCC	3 cut(s)	30, 219, 1059
Lsp1109I	GCAGC	5 cut(s)	149, 253, 457, 947, 1195
LweI	GCATC	3 cut(s)	73, 1104, 1203
MabI	ACCWGGT	1 cut(s)	565
MaeI	CTAG	1 cut(s)	579
MaeII	ACGT	4 cut(s)	111, 457, 1217, 1221
MaeIII	GTNAC	2 cut(s)	462, 628
MalI	GATC	4 cut(s)	178, 299, 549, 810
MboI	GATC	4 cut(s)	176, 297, 547, 808
MboII	GAAGA	6 cut(s)	118, 250, 427, 718, 1166, 1309
MfeI	CAATTG	1 cut(s)	408
MflI	RGATCY	2 cut(s)	176, 547
MlsI	TGGCCA	1 cut(s)	655
MluCI	AATT	7 cut(s)	14, 223, 251, 329, 397, 408, 820
MluNI	TGGCCA	1 cut(s)	655
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	382, 630, 1286
MmeI	TCCRAC	2 cut(s)	303, 1254
MnlI	CCTC	9 cut(s)	38, 42, 88, 93, 207, 386, 624, 912, 1294
Mox20I	TGGCCA	1 cut(s)	655
MscI	TGGCCA	1 cut(s)	655
MseI	TTAA	5 cut(s)	59, 363, 680, 1074, 1305
MslI	CAYNNNNRTG	2 cut(s)	95, 756
Msp20I	TGGCCA	1 cut(s)	655
MspA1I	CMGCKG	1 cut(s)	1045
MspCI	CTTAAG	1 cut(s)	1304
MspR9I	CCNGG	2 cut(s)	567, 917
MunI	CAATTG	1 cut(s)	408
MvaI	CCWGG	2 cut(s)	567, 917
MvnI	CGCG	2 cut(s)	315, 1242
MwoI	GCNNNNNNNGC	4 cut(s)	246, 652, 986, 1092
NarI	GGCGCC	1 cut(s)	5
NcoI	CCATGG	1 cut(s)	656
NdeII	GATC	4 cut(s)	176, 297, 547, 808
NlaIII	CATG	6 cut(s)	94, 340, 557, 660, 1006, 1069
NlaIV	GGNNCC	2 cut(s)	6, 549
NmeAIII	GCCGAG	2 cut(s)	17, 242
NspI	RCATGY	1 cut(s)	557
PaeR7I	CTCGAG	1 cut(s)	390
PagI	TCATGA	1 cut(s)	1002
PceI	AGGCCT	1 cut(s)	1086
PciI	ACATGT	1 cut(s)	553
PfeI	GAWTC	3 cut(s)	504, 595, 608
Pfl23II	CGTACG	1 cut(s)	1218
PflFI	GACNNNGTC	1 cut(s)	567
PkrI	GCNGC	7 cut(s)	139, 268, 472, 718, 962, 1044, 1210
PleI	GAGTC	3 cut(s)	382, 630, 1286
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	382, 630, 1286
Ppu21I	YACGTR	1 cut(s)	1218
PscI	ACATGT	1 cut(s)	553
PsiI	TTATAA	1 cut(s)	293
Psp6I	CCWGG	2 cut(s)	565, 915
PspGI	CCWGG	2 cut(s)	565, 915
PspLI	CGTACG	1 cut(s)	1218
PspN4I	GGNNCC	2 cut(s)	6, 549
PspXI	VCTCGAGB	1 cut(s)	390
PstI	CTGCAG	1 cut(s)	271
PsuI	RGATCY	2 cut(s)	176, 547
PsyI	GACNNNGTC	1 cut(s)	567
RsaI	GTAC	6 cut(s)	68, 104, 805, 857, 969, 1220
RsaNI	GTAC	6 cut(s)	67, 103, 804, 856, 968, 1219
RseI	CAYNNNNRTG	2 cut(s)	95, 756
SaqAI	TTAA	5 cut(s)	59, 363, 680, 1074, 1305
SatI	GCNGC	7 cut(s)	138, 267, 471, 717, 961, 1043, 1209
Sau3AI	GATC	4 cut(s)	176, 297, 547, 808
SchI	GAGTC	3 cut(s)	382, 630, 1286
ScrFI	CCNGG	2 cut(s)	567, 917
SexAI	ACCWGGT	1 cut(s)	565
SfaNI	GCATC	3 cut(s)	73, 1104, 1203
SfcI	CTRYAG	1 cut(s)	267
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	390
SlaI	CTCGAG	1 cut(s)	390
SmiMI	CAYNNNNRTG	2 cut(s)	95, 756
SmlI	CTYRAG	4 cut(s)	390, 1148, 1257, 1304
SmoI	CTYRAG	4 cut(s)	390, 1148, 1257, 1304
Sse9I	AATT	7 cut(s)	14, 223, 251, 329, 397, 408, 820
SseBI	AGGCCT	1 cut(s)	1086
SsiI	CCGC	6 cut(s)	41, 315, 716, 1043, 1113, 1240
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	590
SspMI	CTAG	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	1086
StyD4I	CCNGG	2 cut(s)	565, 915
StyI	CCWWGG	3 cut(s)	656, 812, 1287
TaaI	ACNGT	4 cut(s)	786, 863, 976, 1272
TaiI	ACGT	4 cut(s)	114, 460, 1220, 1224
TaqI	TCGA	5 cut(s)	114, 391, 562, 872, 1295
TasI	AATT	7 cut(s)	14, 223, 251, 329, 397, 408, 820
TatI	WGTACW	1 cut(s)	967
TauI	GCSGC	2 cut(s)	719, 1045
TfiI	GAWTC	3 cut(s)	504, 595, 608
Tru1I	TTAA	5 cut(s)	59, 363, 680, 1074, 1305
Tru9I	TTAA	5 cut(s)	59, 363, 680, 1074, 1305
TscAI	CASTG	3 cut(s)	742, 789, 981
TseI	GCWGC	5 cut(s)	137, 266, 470, 960, 1208
TspDTI	ATGAA	5 cut(s)	244, 464, 792, 1082, 1112
TspGWI	ACGGA	4 cut(s)	8, 84, 357, 468
TspRI	CASTG	3 cut(s)	742, 789, 981
Tth111I	GACNNNGTC	1 cut(s)	567
Vha464I	CTTAAG	1 cut(s)	1304
XapI	RAATTY	1 cut(s)	251
XceI	RCATGY	1 cut(s)	557
XhoI	CTCGAG	1 cut(s)	390
XspI	CTAG	1 cut(s)	579
ZraI	GACGTC	1 cut(s)	112

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.