MD05G1127200.v1.1

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009558
Physical Location & Seq
Forward (+)
25057176 .. 25059692
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1127200.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGCGTCCGTACAATTCCGTACGCTTCTCATCGTCTCGGCGATACTGAGGGTGGTTTTAATCCTCTACGGAGAGTGGCAGGACGCCCACATGGAGGTGAGGTACACAGACATCGATTACATGGTATTCTCAGACGCAGCTTCTCTAATGGCGTCTGGCAAATCCCCATTTGAAAGATCCACTTACAGATATTCTCCTCTCCTCGCCTTTGTGCTCATCCCCAATTCGATTCTCCATCGCTCTTGGGGCAAATTCATCTTCTCAGCTGCAGATTTGCTTGTGGGTTTGTTTATAAGGATCATACTGAAGCTTCGTGGGGTGCCGGAGAAATTGAGCATGATTTCAGTGATTGTTTGGCTCTTTAATCCGTTTACTTTTACGATTGGGACTCGAGGGAATTGTGAGCCAATTGTTTGTGCTTTGGTTCTTTGGGTCTTCATATGTCTTATGAACGGTAAAGGACGTGTGTTACAAGCTGCATTTTGGTATGGGCTTGTTGTCCACTTCAGAATCTACCCCATTATCTATGCACTCCCAATCCTTGTGATTCTTGATCCGCATGTGTTTCAGCCTGGTCAGAAACCTAGACTTCAATATTGGAATCGTCGGCAAGAATCACCTCAAAGCAGTTCTGGTACAAGACTCGCTCAGCTTCTTTGCCCATGGTTTCTTTTGAAAAGTGCATTAACACGAGAAAGGATACTTTTCTCCTTTATATCTGCGGTCGTATTCTTCCTTTTCACTGGTTTATTCTATCATCTGTATGGGTGGGATTTCCTGTACGAGGCTCTGCTGTATCATCTTACCCGTACTGATCCAAGGCACAATTTTTCCATTTACTTTTATCACATCTACCTTCAGTACCACCGTAAACTTTCCATATTGGAGAAACTCGTCTCATTTTTGCCTCAGTTGATAGTCCAGGTTGTTCTTGTCTTGAGTTTCGCCAAAGACCTTCCATTCTGCTGCTTAGTTCAGACAGTGGCATTCGTGGCATTCAACAAGGTAATGACTGCACAATACTTTGTTTGGTTCTTTTGCCTCTTGCCTCTGGTTCTCGCTTGGAGCAACATGAAGCTTAAATGGAAGGGCCTATCGTGCATCGCATTGTGGGTAGGCGGTCAACTTCATTGGTTGTTGTGGGGTTATATGCTTGAGTTCAAGGGACAAAATGTCTTTCTTCAACTCTGGACGGCAAGCATCATATTCTTAGCTGCAAACACGTACGTTTTAGTTTCTCTCATCCGCGATCACAGATTTTCTCCAGTTTTCGCACAGTTGGAGAAAACCGCCAAGGACTCGAAGAAACTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

439

Amino Acids

50.98

Weight (kDa)

9.65

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mannosyl_trans PF05007 128 - 415 5.3e-73 Mannosyltransferase (PIG-M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 319
AccII CGCG 1 cut(s) 1248
AciI CCGC 5 cut(s) 557, 722, 1119, 1246, 1290
AclWI GGATC 4 cut(s) 171, 305, 548, 809
AcsI RAATTY 1 cut(s) 251
AcuI CTGAAG 3 cut(s) 326, 490, 842
AcyI GRCGYC 3 cut(s) 5, 84, 152
AfaI GTAC 8 cut(s) 12, 22, 104, 637, 782, 811, 863, 1226
AfiI CCNNNNNNNGG 2 cut(s) 94, 784
AflIII ACRYGT 2 cut(s) 463, 1221
AgsI TTSAA 6 cut(s) 173, 593, 676, 1000, 1162, 1184
AhdI GACNNNNNGTC 1 cut(s) 1172
AjiI CACGTC 1 cut(s) 464
AjnI CCWGG 2 cut(s) 571, 921
AluBI AGCT 7 cut(s) 140, 266, 310, 476, 652, 1078, 1214
AluI AGCT 7 cut(s) 140, 266, 310, 476, 652, 1078, 1214
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 2 cut(s) 40, 901
AlwI GGATC 4 cut(s) 171, 305, 548, 809
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 1 cut(s) 1090
ApeKI GCWGC 5 cut(s) 137, 266, 476, 966, 1214
ApoI RAATTY 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 1090
AsuHPI GGTGA 2 cut(s) 109, 609
AvaI CYCGRG 1 cut(s) 390
BanI GGYRCC 1 cut(s) 319
BauI CACGAG 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 5 cut(s) 149, 253, 463, 953, 1201
BccI CCATC 1 cut(s) 243
BceAI ACGGC 1 cut(s) 1209
BciT130I CCWGG 2 cut(s) 573, 923
BciVI GTATCC 1 cut(s) 693
BcoDI GTCTC 2 cut(s) 40, 901
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 267
BfuI GTATCC 1 cut(s) 693
BisI GCNGC 5 cut(s) 138, 267, 477, 967, 1215
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 5 cut(s) 139, 268, 478, 968, 1216
Bme1390I CCNGG 2 cut(s) 573, 923
BmeRI GACNNNNNGTC 1 cut(s) 1172
BmeT110I CYCGRG 1 cut(s) 390
BmgBI CACGTC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 1090
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 573, 923
BmsI GCATC 2 cut(s) 1110, 1209
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 1248
Bpu1102I GCTNAGC 1 cut(s) 648
BpuEI CTTGAG 3 cut(s) 958, 1175, 1295
Bsa29I ATCGAT 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 1224
BsaHI GRCGYC 3 cut(s) 5, 84, 152
BsaJI CCNNGG 3 cut(s) 662, 818, 1293
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 784
Bse1I ACTGG 2 cut(s) 748, 1265
BseBI CCWGG 2 cut(s) 573, 923
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 662, 818, 1293
BseGI GGATG 2 cut(s) 216, 1242
BseLI CCNNNNNNNGG 2 cut(s) 94, 784
BseMII CTCAG 5 cut(s) 38, 144, 276, 662, 923
BseNI ACTGG 2 cut(s) 748, 1265
BseRI GAGGAG 2 cut(s) 186, 191
BseXI GCAGC 5 cut(s) 149, 253, 463, 953, 1201
BsgI GTGCAG 1 cut(s) 999
Bsh1236I CGCG 1 cut(s) 1248
Bsh1285I CGRYCG 1 cut(s) 726
BshFI GGCC 1 cut(s) 1092
BshNI GGYRCC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 1 cut(s) 323
BsiWI CGTACG 2 cut(s) 20, 1224
BslFI GGGAC 2 cut(s) 400, 1179
BslI CCNNNNNNNGG 2 cut(s) 94, 784
BsmAI GTCTC 2 cut(s) 40, 901
BsmBI CGTCTC 2 cut(s) 40, 901
BsmFI GGGAC 2 cut(s) 400, 1179
BsmI GAATGC 2 cut(s) 986, 995
BsnI GGCC 1 cut(s) 1092
BsoBI CYCGRG 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 5 cut(s) 176, 297, 553, 814, 1249
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 662
BspACI CCGC 5 cut(s) 557, 722, 1119, 1246, 1290
BspANI GGCC 1 cut(s) 1092
BspCNI CTCAG 5 cut(s) 39, 143, 275, 661, 922
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 1248
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 4 cut(s) 171, 305, 548, 809
BspT107I GGYRCC 1 cut(s) 319
BsrI ACTGG 2 cut(s) 748, 1265
BssECI CCNNGG 3 cut(s) 662, 818, 1293
BssMI GATC 5 cut(s) 176, 297, 553, 814, 1249
BssNI GRCGYC 3 cut(s) 5, 84, 152
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 3 cut(s) 662, 818, 1293
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 2 cut(s) 573, 923
Bst4CI ACNGT 4 cut(s) 455, 869, 982, 1278
BstACI GRCGYC 3 cut(s) 5, 84, 152
BstBAI YACGTR 1 cut(s) 1224
BstC8I GCNNGC 1 cut(s) 1198
BstDEI CTNAG 7 cut(s) 47, 130, 262, 648, 909, 970, 1210
BstDSI CCRYGG 1 cut(s) 662
BstENI CCTNNNNNAGG 1 cut(s) 782
BstF5I GGATG 2 cut(s) 216, 1242
BstFNI CGCG 1 cut(s) 1248
BstKTI GATC 5 cut(s) 179, 300, 556, 817, 1252
BstMAI GTCTC 2 cut(s) 40, 901
BstMBI GATC 5 cut(s) 176, 297, 553, 814, 1249
BstMCI CGRYCG 1 cut(s) 726
BstMWI GCNNNNNNNGC 3 cut(s) 246, 992, 1098
BstNI CCWGG 2 cut(s) 573, 923
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 571, 921
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 1248
BstV1I GCAGC 5 cut(s) 149, 253, 463, 953, 1201
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
Bsu15I ATCGAT 1 cut(s) 114
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 1 cut(s) 1092
BsuTUI ATCGAT 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 662
BtgZI GCGATG 2 cut(s) 221, 1087
BtrI CACGTC 1 cut(s) 464
BtsCI GGATG 2 cut(s) 216, 1242
BtsIMutI CAGTG 3 cut(s) 351, 741, 987
Cac8I GCNNGC 1 cut(s) 1198
Cfr13I GGNCC 1 cut(s) 1090
ClaI ATCGAT 1 cut(s) 114
CseI GACGC 3 cut(s) 92, 141, 143
Csp6I GTAC 8 cut(s) 11, 21, 103, 636, 781, 810, 862, 1225
CviAII CATG 6 cut(s) 91, 121, 337, 560, 663, 1072
CviQI GTAC 8 cut(s) 11, 21, 103, 636, 781, 810, 862, 1225
DdeI CTNAG 7 cut(s) 47, 130, 262, 648, 909, 970, 1210
DpnI GATC 5 cut(s) 178, 299, 555, 816, 1251
DpnII GATC 5 cut(s) 176, 297, 553, 814, 1249
DriI GACNNNNNGTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 1 cut(s) 1172
Eco130I CCWWGG 3 cut(s) 662, 818, 1293
Eco57I CTGAAG 3 cut(s) 326, 490, 842
Eco88I CYCGRG 1 cut(s) 390
EcoNI CCTNNNNNAGG 1 cut(s) 782
EcoO109I RGGNCCY 1 cut(s) 1090
EcoRII CCWGG 2 cut(s) 571, 921
EcoT14I CCWWGG 3 cut(s) 662, 818, 1293
ErhI CCWWGG 3 cut(s) 662, 818, 1293
Esp3I CGTCTC 2 cut(s) 40, 901
FaeI CATG 6 cut(s) 94, 124, 340, 563, 666, 1075
FalI AAGNNNNNCTT 2 cut(s) 166, 198
FaqI GGGAC 2 cut(s) 400, 1179
FatI CATG 6 cut(s) 90, 120, 336, 559, 662, 1071
FauNDI CATATG 1 cut(s) 440
Fnu4HI GCNGC 5 cut(s) 138, 267, 477, 967, 1215
FokI GGATG 2 cut(s) 203, 1229
Fsp4HI GCNGC 5 cut(s) 138, 267, 477, 967, 1215
FspBI CTAG 1 cut(s) 585
GluI GCNGC 5 cut(s) 138, 267, 477, 967, 1215
GsuI CTGGAG 1 cut(s) 1248
HaeIII GGCC 1 cut(s) 1092
HapII CCGG 1 cut(s) 323
HgaI GACGC 3 cut(s) 92, 141, 143
Hin1I GRCGYC 3 cut(s) 5, 84, 152
Hin1II CATG 6 cut(s) 94, 124, 340, 563, 666, 1075
HincII GTYRAC 1 cut(s) 1124
HindII GTYRAC 1 cut(s) 1124
HindIII AAGCTT 2 cut(s) 308, 1076
HinfI GANTC 8 cut(s) 229, 388, 510, 547, 601, 614, 642, 1298
HpaII CCGG 1 cut(s) 323
HphI GGTGA 2 cut(s) 109, 609
Hpy166II GTNNAC 5 cut(s) 105, 372, 502, 872, 1124
Hpy188I TCNGA 4 cut(s) 133, 509, 579, 978
Hpy188III TCNNGA 3 cut(s) 551, 937, 1189
Hpy8I GTNNAC 5 cut(s) 105, 372, 502, 872, 1124
Hpy99I CGWCG 1 cut(s) 609
HpyAV CCTTC 3 cut(s) 866, 965, 1081
HpyCH4III ACNGT 4 cut(s) 455, 869, 982, 1278
HpyCH4IV ACGT 3 cut(s) 463, 1223, 1227
HpyCH4V TGCA 7 cut(s) 269, 479, 530, 683, 1016, 1101, 1217
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 992, 1098
HpyF3I CTNAG 7 cut(s) 47, 130, 262, 648, 909, 970, 1210
HpySE526I ACGT 3 cut(s) 463, 1223, 1227
Hsp92I GRCGYC 3 cut(s) 5, 84, 152
Hsp92II CATG 6 cut(s) 94, 124, 340, 563, 666, 1075
Kzo9I GATC 5 cut(s) 176, 297, 553, 814, 1249
LmnI GCTCC 1 cut(s) 1065
Lsp1109I GCAGC 5 cut(s) 149, 253, 463, 953, 1201
LweI GCATC 2 cut(s) 1110, 1209
MaeI CTAG 1 cut(s) 585
MaeII ACGT 3 cut(s) 463, 1223, 1227
MaeIII GTNAC 1 cut(s) 468
MalI GATC 5 cut(s) 178, 299, 555, 816, 1251
MboI GATC 5 cut(s) 176, 297, 553, 814, 1249
MboII GAAGA 5 cut(s) 250, 427, 724, 1172, 1315
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 7 cut(s) 14, 223, 251, 329, 397, 408, 826
MlyI GAGTC 3 cut(s) 382, 636, 1292
MmeI TCCRAC 1 cut(s) 1260
MseI TTAA 4 cut(s) 59, 363, 686, 1080
MslI CAYNNNNRTG 2 cut(s) 95, 762
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 573, 923
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 2 cut(s) 986, 995
MvaI CCWGG 2 cut(s) 573, 923
MvnI CGCG 1 cut(s) 1248
MwoI GCNNNNNNNGC 3 cut(s) 246, 992, 1098
NcoI CCATGG 1 cut(s) 662
NdeI CATATG 1 cut(s) 440
NdeII GATC 5 cut(s) 176, 297, 553, 814, 1249
NlaIII CATG 6 cut(s) 94, 124, 340, 563, 666, 1075
NlaIV GGNNCC 1 cut(s) 321
NmeAIII GCCGAG 1 cut(s) 17
NspI RCATGY 1 cut(s) 563
PaeR7I CTCGAG 1 cut(s) 390
PctI GAATGC 2 cut(s) 986, 995
PfeI GAWTC 5 cut(s) 229, 510, 547, 601, 614
Pfl23II CGTACG 2 cut(s) 20, 1224
PkrI GCNGC 5 cut(s) 139, 268, 478, 968, 1216
PleI GAGTC 3 cut(s) 382, 636, 1292
PpsI GAGTC 3 cut(s) 382, 636, 1292
Ppu21I YACGTR 1 cut(s) 1224
PsiI TTATAA 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 571, 921
PspGI CCWGG 2 cut(s) 571, 921
PspLI CGTACG 2 cut(s) 20, 1224
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 1090
PspXI VCTCGAGB 1 cut(s) 390
PstI CTGCAG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 8 cut(s) 12, 22, 104, 637, 782, 811, 863, 1226
RsaNI GTAC 8 cut(s) 11, 21, 103, 636, 781, 810, 862, 1225
RseI CAYNNNNRTG 2 cut(s) 95, 762
SaqAI TTAA 4 cut(s) 59, 363, 686, 1080
SatI GCNGC 5 cut(s) 138, 267, 477, 967, 1215
Sau3AI GATC 5 cut(s) 176, 297, 553, 814, 1249
Sau96I GGNCC 1 cut(s) 1090
SchI GAGTC 3 cut(s) 382, 636, 1292
ScrFI CCNGG 2 cut(s) 573, 923
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 2 cut(s) 1110, 1209
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 390
SlaI CTCGAG 1 cut(s) 390
SmiMI CAYNNNNRTG 2 cut(s) 95, 762
SmlI CTYRAG 4 cut(s) 390, 937, 1154, 1310
SmoI CTYRAG 4 cut(s) 390, 937, 1154, 1310
Sse9I AATT 7 cut(s) 14, 223, 251, 329, 397, 408, 826
SsiI CCGC 5 cut(s) 557, 722, 1119, 1246, 1290
SspI AATATT 1 cut(s) 596
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 571, 921
StyI CCWWGG 3 cut(s) 662, 818, 1293
TaaI ACNGT 4 cut(s) 455, 869, 982, 1278
TaiI ACGT 3 cut(s) 466, 1226, 1230
TaqI TCGA 4 cut(s) 114, 227, 391, 1301
TasI AATT 7 cut(s) 14, 223, 251, 329, 397, 408, 826
TfiI GAWTC 5 cut(s) 229, 510, 547, 601, 614
Tru1I TTAA 4 cut(s) 59, 363, 686, 1080
Tru9I TTAA 4 cut(s) 59, 363, 686, 1080
TscAI CASTG 3 cut(s) 351, 748, 987
TseI GCWGC 5 cut(s) 137, 266, 476, 966, 1214
TspDTI ATGAA 5 cut(s) 244, 427, 464, 1088, 1118
TspGWI ACGGA 3 cut(s) 8, 84, 357
TspRI CASTG 3 cut(s) 351, 748, 987
XagI CCTNNNNNAGG 1 cut(s) 782
XapI RAATTY 1 cut(s) 251
XceI RCATGY 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 390
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.