MD10G1130600.v1.1

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009558
Physical Location & Seq
Forward (+)
21112238 .. 21113542
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1130600.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCGCCCATACAATTCCGTGCGCTCCTCATCGTCTCGGCGGTGCTGAGGGTGGTTTTAATCCTGTACGGAGAGTGGCAGGATGCCCACATGGAAGTGAGGTACACAGACATCGATTACATGGTCTTCTCGGACGCAGCTTCTCTAATGGTGTCTGGTAAATCCCCATTTCAGAGATCCACTTACAGATACTCTCCTCTGCTCGCCTTTCTGCTCCTACCCAATTCCATTCTCCATCGCCCTTGGGGCAAATTCATCTTCTCAGCTGCAGGTACTTTATTTGCTTGTGGGTTTGTTTATAAGGATCATACTGAAGCTTCGCGGGGTGCCGGAGAATTGAGCATGATTTCTGTGATTGTTTGGCTCTTTAATCCGTTTACTTTTACGATTGGGACTCGAGGGAATTGTGAGCCAATTGTTTGTGCTTTGGTTCTTTGGGTCTTCATATGTCTTATGAACGGTGCATTGCATTCTGCTATAAGAAGCGTTAGTCAAACTAAATTTTTTGAATGGTTGTTATGGCTTCCGTCCATAACTGTTTTTTTCTTCATGACAGGACGTGTGTTACAAGCTGCATTTTGGTATGGGCTTGTTGTCCACTTCAGAATCTACCCCATTATTCACGCACTCCCCATCCTTATGATTCTGGATCCGCATGTGTTTCGACATGGTCAGAAGCCTAGACTTCAATATTGGAATCGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

236

Amino Acids

27.07

Weight (kDa)

9.57

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mannosyl_trans PF05007 130 - 217 5.9e-08 Mannosyltransferase (PIG-M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 260
AccB1I GGYRCC 2 cut(s) 4, 326
AccII CGCG 1 cut(s) 322
AciI CCGC 3 cut(s) 41, 322, 653
AclWI GGATC 4 cut(s) 171, 312, 644, 657
AcsI RAATTY 2 cut(s) 251, 500
AcuI CTGAAG 2 cut(s) 333, 586
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 68, 104, 274
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 2 cut(s) 509, 689
AjiI CACGTC 1 cut(s) 560
AluBI AGCT 4 cut(s) 140, 266, 317, 572
AluI AGCT 4 cut(s) 140, 266, 317, 572
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 4 cut(s) 171, 312, 644, 657
Ama87I CYCGRG 1 cut(s) 396
ApeKI GCWGC 3 cut(s) 137, 266, 572
ApoI RAATTY 2 cut(s) 251, 500
AspLEI GCGC 2 cut(s) 7, 25
AvaI CYCGRG 1 cut(s) 396
BamHI GGATCC 1 cut(s) 649
BanI GGYRCC 2 cut(s) 4, 326
BbsI GAAGAC 2 cut(s) 118, 433
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 149, 253, 559
BccI CCATC 2 cut(s) 243, 641
BcgI CGANNNNNNTGC 2 cut(s) 644, 678
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 260
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 3 cut(s) 138, 267, 573
BlsI GCNGC 3 cut(s) 139, 268, 574
BmeT110I CYCGRG 1 cut(s) 396
BmgBI CACGTC 1 cut(s) 560
BmiI GGNNCC 3 cut(s) 6, 328, 651
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 2 cut(s) 118, 433
Bpu10I CCTNAGC 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 2 cut(s) 88, 633
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 2 cut(s) 38, 276
BseRI GAGGAG 2 cut(s) 17, 186
BseXI GCAGC 3 cut(s) 149, 253, 559
Bsh1236I CGCG 1 cut(s) 322
BshNI GGYRCC 2 cut(s) 4, 326
BshVI ATCGAT 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 396
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 406
BsmI GAATGC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 396
Bsp143I GATC 3 cut(s) 176, 304, 649
BspACI CCGC 3 cut(s) 41, 322, 653
BspCNI CTCAG 2 cut(s) 39, 275
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 6, 328, 651
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 4 cut(s) 171, 312, 644, 657
BspT107I GGYRCC 2 cut(s) 4, 326
BsrDI GCAATG 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 176, 304, 649
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 461, 538
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 2 cut(s) 47, 262
BstF5I GGATG 2 cut(s) 88, 633
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 25
BstKTI GATC 3 cut(s) 179, 307, 652
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 176, 304, 649
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 659
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 3 cut(s) 149, 253, 559
BstV2I GAAGAC 2 cut(s) 118, 433
BstX2I RGATCY 2 cut(s) 176, 649
BstYI RGATCY 2 cut(s) 176, 649
Bsu15I ATCGAT 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 221
BtrI CACGTC 1 cut(s) 560
BtsCI GGATG 2 cut(s) 88, 633
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 549
CfoI GCGC 2 cut(s) 7, 25
ClaI ATCGAT 1 cut(s) 114
CseI GACGC 1 cut(s) 143
Csp6I GTAC 3 cut(s) 67, 103, 273
CviAII CATG 6 cut(s) 91, 121, 343, 550, 656, 668
CviJI RGCY 9 cut(s) 140, 266, 317, 364, 412, 523, 572, 589, 679
CviKI_1 RGCY 9 cut(s) 140, 266, 317, 364, 412, 523, 572, 589, 679
CviQI GTAC 3 cut(s) 67, 103, 273
DdeI CTNAG 2 cut(s) 47, 262
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 178, 306, 651
DpnII GATC 3 cut(s) 176, 304, 649
Eco130I CCWWGG 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 333, 586
Eco88I CYCGRG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 242
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 242
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 6 cut(s) 94, 124, 346, 553, 659, 671
FaqI GGGAC 1 cut(s) 406
FatI CATG 6 cut(s) 90, 120, 342, 549, 655, 667
FauI CCCGC 1 cut(s) 315
FauNDI CATATG 1 cut(s) 446
Fnu4HI GCNGC 3 cut(s) 138, 267, 573
FokI GGATG 2 cut(s) 95, 620
Fsp4HI GCNGC 3 cut(s) 138, 267, 573
FspBI CTAG 1 cut(s) 681
GlaI GCGC 2 cut(s) 6, 24
GluI GCNGC 3 cut(s) 138, 267, 573
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 330
HgaI GACGC 1 cut(s) 143
HhaI GCGC 2 cut(s) 7, 25
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 94, 124, 346, 553, 659, 671
Hin6I GCGC 2 cut(s) 5, 23
HinP1I GCGC 2 cut(s) 5, 23
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 4 cut(s) 394, 606, 643, 697
HpaII CCGG 1 cut(s) 330
Hpy166II GTNNAC 3 cut(s) 105, 378, 598
Hpy188I TCNGA 4 cut(s) 133, 174, 605, 675
Hpy188III TCNNGA 2 cut(s) 550, 647
Hpy8I GTNNAC 3 cut(s) 105, 378, 598
Hpy99I CGWCG 1 cut(s) 705
HpyCH4III ACNGT 2 cut(s) 461, 538
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 4 cut(s) 269, 464, 469, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 2 cut(s) 47, 262
HpySE526I ACGT 1 cut(s) 559
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 94, 124, 346, 553, 659, 671
HspAI GCGC 2 cut(s) 5, 23
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 176, 304, 649
LmnI GCTCC 2 cut(s) 30, 219
LpnPI CCDG 7 cut(s) 65, 77, 141, 255, 343, 540, 632
Lsp1109I GCAGC 3 cut(s) 149, 253, 559
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 681
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 1 cut(s) 564
MalI GATC 3 cut(s) 178, 306, 651
MboI GATC 3 cut(s) 176, 304, 649
MboII GAAGA 4 cut(s) 118, 250, 433, 538
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 2 cut(s) 176, 649
MluCI AATT 7 cut(s) 14, 223, 251, 335, 403, 414, 500
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 388
MnlI CCTC 5 cut(s) 38, 42, 93, 207, 392
MseI TTAA 2 cut(s) 59, 369
MslI CAYNNNNRTG 2 cut(s) 95, 638
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 330
MunI CAATTG 1 cut(s) 414
Mva1269I GAATGC 1 cut(s) 469
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 246
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 446
NdeII GATC 3 cut(s) 176, 304, 649
NlaIII CATG 6 cut(s) 94, 124, 346, 553, 659, 671
NlaIV GGNNCC 3 cut(s) 6, 328, 651
NmeAIII GCCGAG 1 cut(s) 17
NspI RCATGY 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 396
PagI TCATGA 1 cut(s) 549
PctI GAATGC 1 cut(s) 469
PfeI GAWTC 3 cut(s) 606, 643, 697
PflFI GACNNNGTC 1 cut(s) 669
PkrI GCNGC 3 cut(s) 139, 268, 574
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 388
PsiI TTATAA 1 cut(s) 300
PspN4I GGNNCC 3 cut(s) 6, 328, 651
PspXI VCTCGAGB 1 cut(s) 396
PstI CTGCAG 1 cut(s) 271
PsuI RGATCY 2 cut(s) 176, 649
PsyI GACNNNGTC 1 cut(s) 669
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 3 cut(s) 68, 104, 274
RsaNI GTAC 3 cut(s) 67, 103, 273
RseI CAYNNNNRTG 2 cut(s) 95, 638
SaqAI TTAA 2 cut(s) 59, 369
SatI GCNGC 3 cut(s) 138, 267, 573
Sau3AI GATC 3 cut(s) 176, 304, 649
SchI GAGTC 1 cut(s) 388
SetI ASST 7 cut(s) 104, 142, 268, 274, 319, 562, 574
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 267
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 396
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 95, 638
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 7 cut(s) 14, 223, 251, 335, 403, 414, 500
SsiI CCGC 3 cut(s) 41, 322, 653
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 692
SspMI CTAG 1 cut(s) 681
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 2 cut(s) 461, 538
TaiI ACGT 1 cut(s) 562
TaqI TCGA 3 cut(s) 114, 397, 664
TasI AATT 7 cut(s) 14, 223, 251, 335, 403, 414, 500
TfiI GAWTC 3 cut(s) 606, 643, 697
Tru1I TTAA 2 cut(s) 59, 369
Tru9I TTAA 2 cut(s) 59, 369
TseI GCWGC 3 cut(s) 137, 266, 572
TspDTI ATGAA 4 cut(s) 244, 433, 470, 538
TspGWI ACGGA 4 cut(s) 8, 84, 363, 516
Tth111I GACNNNGTC 1 cut(s) 669
XapI RAATTY 2 cut(s) 251, 500
XceI RCATGY 1 cut(s) 659
XhoI CTCGAG 1 cut(s) 396
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.