Prupe.8G170900_v2.0.a1

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009558
Physical Location & Seq
Forward (+)
17517218 .. 17519544
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G170900.1

Sequence Viewer

Length: 1311 bp
ATGGCGTCCGTACGATTCCGTACGCTCCTCCTGGTCTCGGCGGCGTTGAGGGTGATTCTGATCCTCTACGGAGAGTGGCAGGATACCCACATGGAGGTGAGGTACACAGACATTGATTACATGGTCTTCTCCGACGCTGCTTCTCTAATGGCCGCCGGTAAATCCCCATTTGAAAGATCCACTTACAGGTACTCTCCTCTGCTCGCGTTTTTGCTCATACCCAATTCGTTTCTTCACCGCTCTTGGGGCAAGTTCCTCTTCTCAGCTGCAGATTTGCTTGTGGGTTTGTTTATAAGGACCATATTGAAGCTTCGTGGGGTGCCTGAGAATTTAAGCATGCTTTCTGTGATCGTTTGGCTCTTTAATCCGTTTACCTTTACCATTGGGACTCGTGGGAACTGTGAGCCAATTGTTTGTGCTTTGGTTCTTTGGGTCTTCATATGTCTCATGAACGGACGTGTGTTACAAGCTGCATTTTGGTACGGGCTTGTTGTCCACTTCAGAATCTACCCCATTATCTATGCACTCCCTATCCTTGTGATTCTTGATCCGCATGTGTTTCGATCTGGTCAGAAGCCTTGCCTACAAATTTGGGATCCTCGGCAAGAATCACCTCAAAACAGATCTGTTACAAGACTTGCTCAGCTTCTTTGTCCGTGGTTTCTTTTGAAAAGTGCATTAACACGAGAAAGGATAGTTTTCTCCTTTATATCTGCAGCCGTATTCTTCCTTTTCACTGGTTTATTCTATCATCTATATGGGTGGGAGTTCTTGTATGAGGCACTGCTGTATCATCTTACCCGTACTGATCCAAGGCATAATTTCTCCATTTATTTTTATCACATCTACCTTCAGTACAACCGTAAACTCTCGATATTGGAGAAGCTTGTCTCATTTTTGCCTCAGTTGATAGTCCAGGTTGTGCTTGTTTTGAGTTTTGCGCAAGACCTTCCATTCTGCTGCTTAGTGCAGACAGTAGCATTTGTGGCATTCAACAAGGTAATGACGGCACAATACTTTGTGTGGTTCTTTTGTCTCTTGCCTCTGGTACTCACTTGGAGCAAGATGAAGCTCAAATGGAAAGGCCTATCATGCATGACATTGTGGGTGGCTGGTCAACTTCATTGGTTGATGTGGGGTTATTTGCTTGAGTTCAAGGGACGGAATGTTTTCCTTCAACTCTGGATGGCAAGCATCTTGTTCTTAGCTGCAAACACGTATGTTTTAGTTTCGCTCATCCGCGACCACAGATTTTCTCCAGTTTTCACATGTTTGGAGAAAACTTCCAAGGGCGGGAAGAAACTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

437

Amino Acids

50.87

Weight (kDa)

9.58

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
Acc16I TGCGCA 1 cut(s) 942
AccB1I GGYRCC 1 cut(s) 319
AccBSI CCGCTC 1 cut(s) 240
AccII CGCG 2 cut(s) 206, 1242
AciI CCGC 6 cut(s) 41, 153, 238, 551, 1240, 1293
AclWI GGATC 6 cut(s) 55, 171, 542, 590, 603, 803
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 2 cut(s) 328, 588
AcuI CTGAAG 3 cut(s) 484, 836, 1289
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 8 cut(s) 12, 22, 104, 191, 482, 805, 857, 1050
AfiI CCNNNNNNNGG 5 cut(s) 37, 94, 186, 244, 1293
AflIII ACRYGT 3 cut(s) 457, 1215, 1268
AgsI TTSAA 6 cut(s) 173, 307, 670, 994, 1156, 1178
AjiI CACGTC 1 cut(s) 458
AjnI CCWGG 2 cut(s) 30, 915
AluBI AGCT 7 cut(s) 266, 310, 470, 646, 886, 1072, 1208
AluI AGCT 7 cut(s) 266, 310, 470, 646, 886, 1072, 1208
Alw26I GTCTC 4 cut(s) 40, 449, 895, 1040
AlwI GGATC 6 cut(s) 55, 171, 542, 590, 603, 803
AoxI GGCC 2 cut(s) 150, 1084
ApeKI GCWGC 6 cut(s) 137, 266, 470, 716, 960, 1208
ApoI RAATTY 2 cut(s) 328, 588
Asp700I GAANNNNTTC 1 cut(s) 1169
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 4 cut(s) 64, 109, 227, 603
AvaII GGWCC 1 cut(s) 297
BaeI ACNNNNGTAYC 2 cut(s) 773, 806
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 319
BauI CACGAG 2 cut(s) 390, 684
BbsI GAAGAC 2 cut(s) 118, 427
BbvI GCAGC 6 cut(s) 124, 253, 457, 728, 947, 1195
BccI CCATC 1 cut(s) 1180
BceAI ACGGC 2 cut(s) 704, 1023
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 2 cut(s) 32, 917
BciVI GTATCC 1 cut(s) 76
BcoDI GTCTC 4 cut(s) 40, 449, 895, 1040
BfmI CTRYAG 2 cut(s) 267, 714
BfuI GTATCC 1 cut(s) 76
BglII AGATCT 1 cut(s) 623
BisI GCNGC 8 cut(s) 42, 138, 153, 267, 471, 717, 961, 1209
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 8 cut(s) 43, 139, 154, 268, 472, 718, 962, 1210
Bme1390I CCNGG 2 cut(s) 32, 917
Bme18I GGWCC 1 cut(s) 297
BmgBI CACGTC 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 321, 597
BmrFI CCNGG 2 cut(s) 32, 917
BmsI GCATC 1 cut(s) 1203
BpiI GAAGAC 2 cut(s) 118, 427
BpmI CTGGAG 1 cut(s) 1242
Bpu1102I GCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 1169
BsaAI YACGTR 1 cut(s) 1218
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 4 cut(s) 599, 656, 812, 1287
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 758, 788
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 94, 186, 244, 1293
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 742, 1259
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 2 cut(s) 32, 917
BseDI CCNNGG 4 cut(s) 599, 656, 812, 1287
BseGI GGATG 2 cut(s) 1191, 1236
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 5 cut(s) 37, 94, 186, 244, 1293
BseMII CTCAG 4 cut(s) 276, 315, 656, 917
BseNI ACTGG 2 cut(s) 742, 1259
BseRI GAGGAG 2 cut(s) 17, 186
BseXI GCAGC 6 cut(s) 124, 253, 457, 728, 947, 1195
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 2 cut(s) 206, 1242
BshFI GGCC 2 cut(s) 152, 1086
BshNI GGYRCC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 156
BsiWI CGTACG 2 cut(s) 10, 20
BslFI GGGAC 2 cut(s) 400, 1173
BslI CCNNNNNNNGG 5 cut(s) 37, 94, 186, 244, 1293
BsmAI GTCTC 4 cut(s) 40, 449, 895, 1040
BsmFI GGGAC 2 cut(s) 400, 1173
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 2 cut(s) 152, 1086
Bso31I GGTCTC 1 cut(s) 40
Bsp143I GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 6 cut(s) 41, 153, 238, 551, 1240, 1293
BspANI GGCC 2 cut(s) 152, 1086
BspCNI CTCAG 4 cut(s) 275, 316, 655, 916
BspFNI CGCG 2 cut(s) 206, 1242
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 2 cut(s) 321, 597
BspMAI CTGCAG 2 cut(s) 271, 718
BspPI GGATC 6 cut(s) 55, 171, 542, 590, 603, 803
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 2 cut(s) 742, 1259
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 4 cut(s) 599, 656, 812, 1287
BssMI GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 390, 684
BssT1I CCWWGG 2 cut(s) 812, 1287
Bst2BI CACGAG 2 cut(s) 390, 684
Bst2UI CCWGG 2 cut(s) 32, 917
Bst4CI ACNGT 3 cut(s) 401, 863, 976
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 1218
BstC8I GCNNGC 3 cut(s) 204, 338, 1192
BstDEI CTNAG 6 cut(s) 262, 324, 642, 903, 964, 1204
BstDSI CCRYGG 1 cut(s) 656
BstF5I GGATG 2 cut(s) 1191, 1236
BstFNI CGCG 2 cut(s) 206, 1242
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 8 cut(s) 63, 179, 351, 550, 566, 598, 626, 811
BstMAI GTCTC 4 cut(s) 40, 449, 895, 1040
BstMBI GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
BstMWI GCNNNNNNNGC 3 cut(s) 246, 986, 1092
BstNI CCWGG 2 cut(s) 32, 917
BstNSI RCATGY 3 cut(s) 340, 557, 1272
BstSCI CCNGG 2 cut(s) 30, 915
BstSFI CTRYAG 2 cut(s) 267, 714
BstUI CGCG 2 cut(s) 206, 1242
BstV1I GCAGC 6 cut(s) 124, 253, 457, 728, 947, 1195
BstV2I GAAGAC 2 cut(s) 118, 427
BstX2I RGATCY 3 cut(s) 176, 595, 623
BstYI RGATCY 3 cut(s) 176, 595, 623
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 152, 1086
BtgI CCRYGG 1 cut(s) 656
BtrI CACGTC 1 cut(s) 458
BtsCI GGATG 2 cut(s) 1191, 1236
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 2 cut(s) 735, 782
Cac8I GCNNGC 3 cut(s) 204, 338, 1192
CciI TCATGA 1 cut(s) 447
CfoI GCGC 1 cut(s) 943
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 1 cut(s) 143
Csp6I GTAC 8 cut(s) 11, 21, 103, 190, 481, 804, 856, 1049
CviAII CATG 8 cut(s) 91, 121, 337, 448, 554, 1092, 1096, 1269
CviQI GTAC 8 cut(s) 11, 21, 103, 190, 481, 804, 856, 1049
DdeI CTNAG 6 cut(s) 262, 324, 642, 903, 964, 1204
DpnI GATC 8 cut(s) 62, 178, 350, 549, 565, 597, 625, 810
DpnII GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 812, 1287
Eco147I AGGCCT 1 cut(s) 1086
Eco31I GGTCTC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 297
Eco57I CTGAAG 3 cut(s) 484, 836, 1289
EcoRII CCWGG 2 cut(s) 30, 915
EcoT14I CCWWGG 2 cut(s) 812, 1287
EcoT22I ATGCAT 1 cut(s) 1097
ErhI CCWWGG 2 cut(s) 812, 1287
FaeI CATG 8 cut(s) 94, 124, 340, 451, 557, 1095, 1099, 1272
FalI AAGNNNNNCTT 4 cut(s) 166, 198, 242, 274
FaqI GGGAC 2 cut(s) 400, 1173
FatI CATG 8 cut(s) 90, 120, 336, 447, 553, 1091, 1095, 1268
FauI CCCGC 1 cut(s) 1286
FauNDI CATATG 1 cut(s) 440
Fnu4HI GCNGC 8 cut(s) 42, 138, 153, 267, 471, 717, 961, 1209
FokI GGATG 2 cut(s) 1198, 1223
Fsp4HI GCNGC 8 cut(s) 42, 138, 153, 267, 471, 717, 961, 1209
FspI TGCGCA 1 cut(s) 942
GlaI GCGC 1 cut(s) 942
GluI GCNGC 8 cut(s) 42, 138, 153, 267, 471, 717, 961, 1209
GsuI CTGGAG 1 cut(s) 1242
HaeIII GGCC 2 cut(s) 152, 1086
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 943
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 94, 124, 340, 451, 557, 1095, 1099, 1272
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HincII GTYRAC 1 cut(s) 1118
HindII GTYRAC 1 cut(s) 1118
HindIII AAGCTT 2 cut(s) 308, 884
HinfI GANTC 6 cut(s) 15, 55, 388, 504, 541, 608
HpaII CCGG 1 cut(s) 156
HphI GGTGA 4 cut(s) 64, 109, 227, 603
Hpy166II GTNNAC 5 cut(s) 105, 372, 496, 866, 1118
Hpy188I TCNGA 4 cut(s) 60, 133, 503, 573
Hpy188III TCNNGA 4 cut(s) 448, 545, 871, 1183
Hpy8I GTNNAC 5 cut(s) 105, 372, 496, 866, 1118
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 3 cut(s) 860, 959, 1184
HpyCH4III ACNGT 3 cut(s) 401, 863, 976
HpyCH4IV ACGT 2 cut(s) 457, 1217
HpyCH4V TGCA 8 cut(s) 269, 473, 524, 677, 716, 970, 1095, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 986, 1092
HpyF3I CTNAG 6 cut(s) 262, 324, 642, 903, 964, 1204
HpySE526I ACGT 2 cut(s) 457, 1217
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 94, 124, 340, 451, 557, 1095, 1099, 1272
HspAI GCGC 1 cut(s) 941
Kzo9I GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
LmnI GCTCC 2 cut(s) 30, 1059
Lsp1109I GCAGC 6 cut(s) 124, 253, 457, 728, 947, 1195
LweI GCATC 1 cut(s) 1203
MaeII ACGT 2 cut(s) 457, 1217
MaeIII GTNAC 2 cut(s) 462, 628
MalI GATC 8 cut(s) 62, 178, 350, 549, 565, 597, 625, 810
MbiI CCGCTC 1 cut(s) 240
MboI GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
MboII GAAGA 6 cut(s) 118, 224, 250, 427, 718, 1309
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 3 cut(s) 176, 595, 623
MluCI AATT 5 cut(s) 223, 328, 408, 588, 820
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 1 cut(s) 1097
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 3 cut(s) 332, 363, 680
MslI CAYNNNNRTG 2 cut(s) 95, 756
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 32, 917
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 2 cut(s) 32, 917
MvnI CGCG 2 cut(s) 206, 1242
MwoI GCNNNNNNNGC 3 cut(s) 246, 986, 1092
NdeI CATATG 1 cut(s) 440
NdeII GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
NlaIII CATG 8 cut(s) 94, 124, 340, 451, 557, 1095, 1099, 1272
NlaIV GGNNCC 2 cut(s) 321, 597
NmeAIII GCCGAG 2 cut(s) 17, 580
NsbI TGCGCA 1 cut(s) 942
NsiI ATGCAT 1 cut(s) 1097
NspI RCATGY 3 cut(s) 340, 557, 1272
PaeI GCATGC 1 cut(s) 340
PagI TCATGA 1 cut(s) 447
PceI AGGCCT 1 cut(s) 1086
PciI ACATGT 1 cut(s) 1268
PctI GAATGC 1 cut(s) 989
PdmI GAANNNNTTC 1 cut(s) 1169
PfeI GAWTC 5 cut(s) 15, 55, 504, 541, 608
Pfl23II CGTACG 2 cut(s) 10, 20
PkrI GCNGC 8 cut(s) 43, 139, 154, 268, 472, 718, 962, 1210
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 1218
PscI ACATGT 1 cut(s) 1268
PsiI TTATAA 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 30, 915
PspGI CCWGG 2 cut(s) 30, 915
PspLI CGTACG 2 cut(s) 10, 20
PspN4I GGNNCC 2 cut(s) 321, 597
PspPI GGNCC 1 cut(s) 297
PstI CTGCAG 2 cut(s) 271, 718
PsuI RGATCY 3 cut(s) 176, 595, 623
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 8 cut(s) 12, 22, 104, 191, 482, 805, 857, 1050
RsaNI GTAC 8 cut(s) 11, 21, 103, 190, 481, 804, 856, 1049
RseI CAYNNNNRTG 2 cut(s) 95, 756
SaqAI TTAA 3 cut(s) 332, 363, 680
SatI GCNGC 8 cut(s) 42, 138, 153, 267, 471, 717, 961, 1209
Sau3AI GATC 8 cut(s) 60, 176, 348, 547, 563, 595, 623, 808
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 32, 917
SfaNI GCATC 1 cut(s) 1203
SfcI CTRYAG 2 cut(s) 267, 714
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 2 cut(s) 95, 756
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
SphI GCATGC 1 cut(s) 340
Sse9I AATT 5 cut(s) 223, 328, 408, 588, 820
SseBI AGGCCT 1 cut(s) 1086
SsiI CCGC 6 cut(s) 41, 153, 238, 551, 1240, 1293
StuI AGGCCT 1 cut(s) 1086
StyD4I CCNGG 2 cut(s) 30, 915
StyI CCWWGG 2 cut(s) 812, 1287
TaaI ACNGT 3 cut(s) 401, 863, 976
TaiI ACGT 2 cut(s) 460, 1220
TaqI TCGA 2 cut(s) 562, 872
TasI AATT 5 cut(s) 223, 328, 408, 588, 820
TatI WGTACW 1 cut(s) 855
TauI GCSGC 2 cut(s) 44, 155
TfiI GAWTC 5 cut(s) 15, 55, 504, 541, 608
Tru1I TTAA 3 cut(s) 332, 363, 680
Tru9I TTAA 3 cut(s) 332, 363, 680
TscAI CASTG 2 cut(s) 742, 789
TseI GCWGC 6 cut(s) 137, 266, 470, 716, 960, 1208
TspDTI ATGAA 4 cut(s) 427, 464, 1082, 1112
TspGWI ACGGA 6 cut(s) 8, 84, 357, 468, 645, 1177
TspRI CASTG 2 cut(s) 742, 789
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 2 cut(s) 328, 588
XceI RCATGY 3 cut(s) 340, 557, 1272
XmnI GAANNNNTTC 1 cut(s) 1169
Zsp2I ATGCAT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.