AT5G25170

deSI-like protein At4g17486

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010449
Physical Location & Seq
Forward (+)
8693012 .. 8695095
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25170.2

Sequence Viewer

Length: 657 bp
ATGTTGTGTAGAATGGTGGTGGTGACTGGCCGGAAAAAGAAACCCGGTTCGGTTCCGGTTTATCTGAATGTATACGATCTCACACCCATCAATGGCTACGCTTACTGGTTAGGACTTGGGATCTATCACTCTGGTGTTGAAGTTCATGGAGTTGAATATGGTTTTGGAGCTCACGATCATTCGACTACCGGGATATTCGAGGTGGAACCGAAGCAATGTCCGGGTTTCACATTTAGGAAGTCTATATTGATTGGAAGAACTGATTTAGATCCTGAAAACGTTCGTGTCTTTATGGAGAAACTTGCGGAAGAATATAGTGGAAACAGTTATCATTTGATTACTAAGAATTGCAATCACTTTTGCAATGATGTTTGTGTGCAACTGACTCGAAGATCGATCCCTAGTTGGGTTAACCGTCTTGCTCGCTTTGGTTTGTTTTGCAACTGTGTTCTGCCGGCGGAGTTGAATGAGACTAAGGTGAGGCAGGTGAGATCAAAGGAAGAGAAGATTCCGGAAGTAGAGAAGAAGAAACTTCGGAGTAGATCAAGTAGGTTTCCACCTGGTCCTTCGCTTTCTTCGTCGGGTTCTTTAAACCGAAGCAGAAGAGGAGAAAGGAGAAGACAATGTCTTCCTCCATCACCAACTGTGAGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

218

Amino Acids

24.69

Weight (kDa)

9.57

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 2 - 110 2e-42 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
Acc36I ACCTGC 1 cut(s) 475
AccI GTMKAC 1 cut(s) 72
AccIII TCCGGA 1 cut(s) 511
AciI CCGC 2 cut(s) 305, 458
AclI AACGTT 1 cut(s) 279
AclWI GGATC 3 cut(s) 128, 263, 391
AcoI YGGCCR 1 cut(s) 28
AfiI CCNNNNNNNGG 2 cut(s) 92, 406
AgsI TTSAA 3 cut(s) 140, 155, 466
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AleI CACNNNNGTG 1 cut(s) 132
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 3 cut(s) 128, 263, 391
Aor13HI TCCGGA 1 cut(s) 511
AoxI GGCC 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 3 cut(s) 45, 190, 222
AsuHPI GGTGA 4 cut(s) 34, 490, 499, 630
AvaII GGWCC 1 cut(s) 563
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 620, 625
Bbv12I GWGCWC 1 cut(s) 172
BccI CCATC 2 cut(s) 95, 643
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 561
BcnI CCSGG 3 cut(s) 45, 190, 222
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 475
Bme1390I CCNGG 4 cut(s) 45, 190, 222, 561
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 2 cut(s) 54, 207
BmrFI CCNGG 4 cut(s) 45, 190, 222, 561
BpiI GAAGAC 2 cut(s) 620, 625
BpuMI CCSGG 3 cut(s) 45, 190, 222
Bsa29I ATCGAT 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 267
BsaWI WCCGGW 2 cut(s) 55, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 406
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 2 cut(s) 31, 110
Bse3DI GCAATG 2 cut(s) 221, 370
Bse8I GATNNNNATC 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 511
BseBI CCWGG 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 395
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 2 cut(s) 92, 406
BseMI GCAATG 2 cut(s) 221, 370
BseNI ACTGG 2 cut(s) 31, 110
BseRI GAGGAG 1 cut(s) 621
BshFI GGCC 1 cut(s) 30
BshVI ATCGAT 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 172
BsiSI CCGG 7 cut(s) 31, 45, 56, 189, 221, 455, 512
BslI CCNNNNNNNGG 2 cut(s) 92, 406
BsmAI GTCTC 1 cut(s) 464
BsnI GGCC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 172
Bsp13I TCCGGA 1 cut(s) 511
Bsp143I GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
BspACI CCGC 2 cut(s) 305, 458
BspANI GGCC 1 cut(s) 30
BspDI ATCGAT 1 cut(s) 395
BspEI TCCGGA 1 cut(s) 511
BspLI GGNNCC 2 cut(s) 54, 207
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 3 cut(s) 128, 263, 391
BsrDI GCAATG 2 cut(s) 221, 370
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 2 cut(s) 31, 110
BssAI RCCGGY 1 cut(s) 454
BssMI GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
BssNAI GTATAC 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 4 cut(s) 326, 416, 446, 646
Bst6I CTCTTC 2 cut(s) 495, 598
BstC8I GCNNGC 2 cut(s) 424, 456
BstDEI CTNAG 2 cut(s) 342, 474
BstKTI GATC 8 cut(s) 79, 123, 178, 271, 395, 399, 494, 545
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
BstNI CCWGG 1 cut(s) 561
BstSCI CCNGG 4 cut(s) 43, 188, 220, 559
BstV2I GAAGAC 2 cut(s) 620, 625
BstX2I RGATCY 2 cut(s) 120, 268
BstYI RGATCY 2 cut(s) 120, 268
BstZ17I GTATAC 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 395
BsuRI GGCC 1 cut(s) 30
BsuTUI ATCGAT 1 cut(s) 395
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 424, 456
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 395
CsiI ACCWGGT 1 cut(s) 559
CviAII CATG 1 cut(s) 146
CviJI RGCY 3 cut(s) 30, 96, 170
CviKI_1 RGCY 3 cut(s) 30, 96, 170
DdeI CTNAG 2 cut(s) 342, 474
DpnI GATC 8 cut(s) 78, 122, 177, 270, 394, 398, 493, 544
DpnII GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
DraI TTTAAA 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 495, 598
EarI CTCTTC 2 cut(s) 495, 598
EciI GGCGGA 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 563
Eco53kI GAGCTC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 172
FaeI CATG 1 cut(s) 149
FaiI YATR 6 cut(s) 73, 147, 159, 245, 293, 315
FatI CATG 1 cut(s) 145
FblI GTMKAC 1 cut(s) 72
FriOI GRGCYC 1 cut(s) 172
FspBI CTAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 30
HapII CCGG 7 cut(s) 31, 45, 56, 189, 221, 455, 512
Hin1II CATG 1 cut(s) 149
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 2 cut(s) 385, 508
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 7 cut(s) 31, 45, 56, 189, 221, 455, 512
HphI GGTGA 4 cut(s) 34, 490, 499, 630
Hpy166II GTNNAC 2 cut(s) 73, 412
Hpy188I TCNGA 2 cut(s) 66, 537
Hpy188III TCNNGA 3 cut(s) 173, 272, 512
Hpy8I GTNNAC 2 cut(s) 73, 412
Hpy99I CGWCG 1 cut(s) 583
HpyAV CCTTC 1 cut(s) 576
HpyCH4III ACNGT 4 cut(s) 326, 416, 446, 646
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 351, 363, 379, 441
HpyF3I CTNAG 2 cut(s) 342, 474
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 1 cut(s) 149
Kpn2I TCCGGA 1 cut(s) 511
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
LmnI GCTCC 1 cut(s) 167
MabI ACCWGGT 1 cut(s) 559
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 22
MalI GATC 8 cut(s) 78, 122, 177, 270, 394, 398, 493, 544
MboI GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
MflI RGATCY 2 cut(s) 120, 268
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 346
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 4 cut(s) 193, 474, 599, 642
MroI TCCGGA 1 cut(s) 511
MroNI GCCGGC 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 2 cut(s) 411, 590
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 7 cut(s) 31, 45, 56, 189, 221, 455, 512
MspR9I CCNGG 4 cut(s) 45, 190, 222, 561
MvaI CCWGG 1 cut(s) 561
NaeI GCCGGC 1 cut(s) 456
NciI CCSGG 3 cut(s) 45, 190, 222
NdeII GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 1 cut(s) 149
NlaIV GGNNCC 2 cut(s) 54, 207
NmuCI GTSAC 1 cut(s) 22
OliI CACNNNNGTG 1 cut(s) 132
PaqCI CACCTGC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PdiI GCCGGC 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 508
PflFI GACNNNGTC 1 cut(s) 624
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 172
Psp1406I AACGTT 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 2 cut(s) 54, 207
PspPI GGNCC 1 cut(s) 563
PsuI RGATCY 2 cut(s) 120, 268
PsyI GACNNNGTC 1 cut(s) 624
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 2 cut(s) 411, 590
Sau3AI GATC 8 cut(s) 76, 120, 175, 268, 392, 396, 491, 542
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 4 cut(s) 45, 190, 222, 561
SduI GDGCHC 1 cut(s) 172
SetI ASST 7 cut(s) 172, 204, 282, 480, 489, 554, 562
SexAI ACCWGGT 1 cut(s) 559
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 1 cut(s) 346
SsiI CCGC 2 cut(s) 305, 458
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 4 cut(s) 43, 188, 220, 559
TaaI ACNGT 4 cut(s) 326, 416, 446, 646
TaiI ACGT 1 cut(s) 282
TaqI TCGA 4 cut(s) 182, 198, 388, 395
TasI AATT 1 cut(s) 346
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 2 cut(s) 411, 590
Tru9I TTAA 2 cut(s) 411, 590
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 134
Tth111I GACNNNGTC 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.