MD08G1059400.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010449
Physical Location & Seq
Forward (+)
4747962 .. 4750576
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1059400.v1.1.491

Sequence Viewer

Length: 690 bp
ATGTTGTGTAATATGATACTGGTGCCTAGAAAGAAGAAGATTGGGAAGGTGCCGGTTTATCTGAACGTGTACGATCTTACGCCGATCAATGGGTACGCGTATTGGCTTGGTCTCGGAGTCTACCATTCCGGCGTCCAAGTACATGGCGTTGAATATGCTTTTGGAGCTCATGAGCATGCAACGACTGGGATTTTCGAAGTGGAACCGAAGCAATGTCCCGGCTTTGTGTTTAGAAAATCGATTTTGATCGGAAGAACGGATTTAGGTCCGAAGGACATCCGTGCCTTTATGGAGAAACTAGCAGAGGAGTACTCCGGAAACACATACCATCTTATCACCAAGAATTGCAACCACTTCTGCAATGATGTTTGCACCAGATTGACGAGGAAGCCTATTCCTCGCTGGGTCAACCGCCTTGCTCGACTTGGTTTCTTTTGCAATTGTGTTCTTCCGGCTGGTCTGAACGAAACAAAGGTTCAGCAAGTAAGATCAGACGGGGTTTATAACGGAGACAAGAAGAAACTGAGAAGCCATTCGACTAATAATGCATCCACAAATCCTCGGTCCTCTTCATTAAAATCTTCCACTGCAGCGTCTACAGGCAGAAGTAGTAGACAAAGACACTGCGTTCCTCCGTCTCAATCTTTGATCCATTCTTCCTCAACCTCGGCATTGACTCTAAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

230

Amino Acids

25.5

Weight (kDa)

9.76

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 18 - 152 5.2e-54 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AccB1I GGYRCC 2 cut(s) 22, 49
AccI GTMKAC 3 cut(s) 120, 596, 613
AccII CGCG 1 cut(s) 98
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 1 cut(s) 412
AclWI GGATC 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 4 cut(s) 71, 95, 141, 311
AfiI CCNNNNNNNGG 1 cut(s) 89
AflIII ACRYGT 2 cut(s) 66, 96
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 2 cut(s) 167, 685
AluI AGCT 2 cut(s) 167, 685
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 3 cut(s) 116, 504, 642
AlwI GGATC 1 cut(s) 643
Aor13HI TCCGGA 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 532
AspS9I GGNCC 2 cut(s) 266, 564
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 195
AvaII GGWCC 2 cut(s) 266, 564
BanI GGYRCC 2 cut(s) 22, 49
BanII GRGCYC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 602
BccI CCATC 1 cut(s) 336
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 3 cut(s) 116, 504, 642
BfaI CTAG 2 cut(s) 27, 299
BfmI CTRYAG 2 cut(s) 588, 597
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 2 cut(s) 266, 564
BmgT120I GGNCC 2 cut(s) 266, 564
BmiI GGNNCC 3 cut(s) 24, 51, 204
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu14I TTCGAA 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 560, 666
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 2 cut(s) 24, 190
Bse3DI GCAATG 2 cut(s) 218, 367
Bse8I GATNNNNATC 1 cut(s) 245
BseAI TCCGGA 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 560, 666
BseGI GGATG 2 cut(s) 276, 548
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 2 cut(s) 218, 367
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 2 cut(s) 24, 190
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 602
BseYI CCCAGC 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 98
BshNI GGYRCC 2 cut(s) 22, 49
BshVI ATCGAT 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 5 cut(s) 53, 129, 219, 315, 452
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 89
BsmAI GTCTC 3 cut(s) 116, 504, 642
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 169
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 5 cut(s) 73, 84, 246, 488, 648
BspACI CCGC 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 516
BspDI ATCGAT 1 cut(s) 239
BspEI TCCGGA 1 cut(s) 314
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 3 cut(s) 24, 51, 204
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 1 cut(s) 643
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 2 cut(s) 22, 49
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 218, 367
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 2 cut(s) 24, 190
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 560, 666
BssMI GATC 5 cut(s) 73, 84, 246, 488, 648
BssNI GRCGYC 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 574
BstACI GRCGYC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 524
BstF5I GGATG 2 cut(s) 276, 548
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 5 cut(s) 76, 87, 249, 491, 651
BstMAI GTCTC 3 cut(s) 116, 504, 642
BstMBI GATC 5 cut(s) 73, 84, 246, 488, 648
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 2 cut(s) 588, 597
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 602
BstXI CCANNNNNNTGG 1 cut(s) 143
Bsu15I ATCGAT 1 cut(s) 239
BsuTUI ATCGAT 1 cut(s) 239
BtsCI GGATG 2 cut(s) 276, 548
BtsI GCAGTG 2 cut(s) 585, 622
BtsIMutI CAGTG 2 cut(s) 585, 622
Cac8I GCNNGC 1 cut(s) 177
CciI TCATGA 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 266, 564
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 2 cut(s) 121, 582
Csp6I GTAC 4 cut(s) 70, 94, 140, 310
CviAII CATG 3 cut(s) 143, 170, 176
CviJI RGCY 7 cut(s) 106, 167, 222, 391, 455, 531, 685
CviKI_1 RGCY 7 cut(s) 106, 167, 222, 391, 455, 531, 685
CviQI GTAC 4 cut(s) 70, 94, 140, 310
DdeI CTNAG 1 cut(s) 524
DpnI GATC 5 cut(s) 75, 86, 248, 490, 650
DpnII GATC 5 cut(s) 73, 84, 246, 488, 648
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
Ecl136II GAGCTC 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 266, 564
Eco53kI GAGCTC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 167
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 169
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 3 cut(s) 146, 173, 179
FaiI YATR 8 cut(s) 14, 144, 156, 171, 177, 290, 325, 504
FaqI GGGAC 1 cut(s) 201
FatI CATG 3 cut(s) 142, 169, 175
FblI GTMKAC 3 cut(s) 120, 596, 613
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 2 cut(s) 263, 535
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 2 cut(s) 27, 299
GluI GCNGC 1 cut(s) 591
GsaI CCCAGC 1 cut(s) 406
HapII CCGG 5 cut(s) 53, 129, 219, 315, 452
HgaI GACGC 2 cut(s) 121, 582
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 3 cut(s) 146, 173, 179
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 2 cut(s) 117, 676
HpaII CCGG 5 cut(s) 53, 129, 219, 315, 452
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 5 cut(s) 70, 121, 409, 597, 614
Hpy188I TCNGA 6 cut(s) 63, 116, 251, 270, 462, 493
Hpy188III TCNNGA 2 cut(s) 170, 315
Hpy8I GTNNAC 5 cut(s) 70, 121, 409, 597, 614
HpyAV CCTTC 2 cut(s) 40, 265
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 7 cut(s) 179, 348, 360, 372, 438, 548, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 524
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 3 cut(s) 146, 173, 179
Kpn2I TCCGGA 1 cut(s) 314
Kzo9I GATC 5 cut(s) 73, 84, 246, 488, 648
LmnI GCTCC 1 cut(s) 164
Lsp1109I GCAGC 1 cut(s) 602
LweI GCATC 1 cut(s) 557
MaeI CTAG 2 cut(s) 27, 299
MaeII ACGT 1 cut(s) 66
MalI GATC 5 cut(s) 75, 86, 248, 490, 650
MboI GATC 5 cut(s) 73, 84, 246, 488, 648
MboII GAAGA 8 cut(s) 46, 49, 264, 440, 529, 561, 573, 648
MfeI CAATTG 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 343, 439
MluI ACGCGT 1 cut(s) 96
MlyI GAGTC 2 cut(s) 126, 670
MnlI CCTC 8 cut(s) 298, 378, 408, 570, 577, 642, 670, 676
Mph1103I ATGCAT 1 cut(s) 550
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 532
MseI TTAA 1 cut(s) 575
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 5 cut(s) 53, 129, 219, 315, 452
MspR9I CCNGG 1 cut(s) 219
MunI CAATTG 1 cut(s) 439
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 164
NciI CCSGG 1 cut(s) 219
NdeII GATC 5 cut(s) 73, 84, 246, 488, 648
NlaIII CATG 3 cut(s) 146, 173, 179
NlaIV GGNNCC 3 cut(s) 24, 51, 204
NmeAIII GCCGAG 1 cut(s) 647
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 1 cut(s) 195
PaeI GCATGC 1 cut(s) 179
PagI TCATGA 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 532
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 2 cut(s) 125, 670
PpsI GAGTC 2 cut(s) 125, 670
PsiI TTATAA 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 402
PspN4I GGNNCC 3 cut(s) 24, 51, 204
PspPI GGNCC 2 cut(s) 266, 564
PstI CTGCAG 1 cut(s) 592
RsaI GTAC 4 cut(s) 71, 95, 141, 311
RsaNI GTAC 4 cut(s) 70, 94, 140, 310
RseI CAYNNNNRTG 1 cut(s) 174
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 5 cut(s) 73, 84, 246, 488, 648
Sau96I GGNCC 2 cut(s) 266, 564
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 126, 670
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 1 cut(s) 169
SetI ASST 7 cut(s) 51, 69, 169, 268, 477, 668, 687
SfaNI GCATC 1 cut(s) 557
SfcI CTRYAG 2 cut(s) 588, 597
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 2 cut(s) 266, 564
SmiMI CAYNNNNRTG 1 cut(s) 174
SphI GCATGC 1 cut(s) 179
Sse9I AATT 2 cut(s) 343, 439
SsiI CCGC 1 cut(s) 412
SspMI CTAG 2 cut(s) 27, 299
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 217
TaiI ACGT 1 cut(s) 69
TaqI TCGA 4 cut(s) 195, 239, 421, 536
TaqII GACCGA 1 cut(s) 552
TasI AATT 2 cut(s) 343, 439
TatI WGTACW 2 cut(s) 139, 309
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 2 cut(s) 592, 629
TseI GCWGC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 561
TspGWI ACGGA 4 cut(s) 269, 272, 522, 624
TspRI CASTG 2 cut(s) 592, 629
VpaK11BI GGWCC 2 cut(s) 266, 564
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 3 cut(s) 120, 596, 613
XmnI GAANNNNTTC 1 cut(s) 532
XspI CTAG 2 cut(s) 27, 299
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.