pycom08g04950

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010449
Physical Location & Seq
Forward (+)
3790077 .. 3790899
823 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04950.1

Sequence Viewer

Length: 402 bp
ATGGAGAAACGAGCAGAGGAGTACTCCGGAAACACATACCATCTTATCACCAAGAACTGCAACCACTTCTGCAATGATGTTTGCACCAGATTGACGGGGAAGCCTATTCCTCGCTGGGTCAACCGCCTTGCTCGACTTGGTTTCTTTTGCAATTGTGTTCTTCCGGCTGGTCTGAACGAAACAAAGGTTCGGCAAGTAAGATCAGACGGGGTTTATAATGGAGACAAGAAGAAACTGAGAAGCCATTCGACTAATAATGCATCCTCAAATCCTCGGCCCTCTTCATTAAAATCTTCCACTGCAGCGTCTACAGGCAGAAGTAGTAGACAAAGACACTGCGTTCCTCCGTCTCAATCTTTGATCCATTCTTCCTCAACCTCGGCATTGACTCTAAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

134

Amino Acids

14.73

Weight (kDa)

10.18

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 4 - 56 1.5e-20 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AccI GTMKAC 2 cut(s) 308, 325
AccIII TCCGGA 1 cut(s) 26
AciI CCGC 1 cut(s) 124
AclWI GGATC 1 cut(s) 355
AfaI GTAC 1 cut(s) 23
AluBI AGCT 1 cut(s) 397
AluI AGCT 1 cut(s) 397
Alw26I GTCTC 2 cut(s) 216, 354
AlwI GGATC 1 cut(s) 355
Aor13HI TCCGGA 1 cut(s) 26
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 40
BbvI GCAGC 1 cut(s) 314
BccI CCATC 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 216, 354
BfmI CTRYAG 2 cut(s) 300, 309
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
BmcAI AGTACT 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 276
BmsI GCATC 1 cut(s) 269
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BsaJI CCNNGG 2 cut(s) 272, 378
BsaWI WCCGGW 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 79
BseAI TCCGGA 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 272, 378
BseGI GGATG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 314
BseYI CCCAGC 1 cut(s) 114
BshFI GGCC 1 cut(s) 277
BsiSI CCGG 2 cut(s) 27, 164
BsmAI GTCTC 2 cut(s) 216, 354
BsmBI CGTCTC 1 cut(s) 354
BsnI GGCC 1 cut(s) 277
Bsp13I TCCGGA 1 cut(s) 26
Bsp143I GATC 2 cut(s) 200, 360
BspACI CCGC 1 cut(s) 124
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 228
BspEI TCCGGA 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 272, 378
BssMI GATC 2 cut(s) 200, 360
Bst6I CTCTTC 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 260
BstKTI GATC 2 cut(s) 203, 363
BstMAI GTCTC 2 cut(s) 216, 354
BstMBI GATC 2 cut(s) 200, 360
BstSFI CTRYAG 2 cut(s) 300, 309
BstV1I GCAGC 1 cut(s) 314
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 260
BtsI GCAGTG 2 cut(s) 297, 334
BtsIMutI CAGTG 2 cut(s) 297, 334
Cfr13I GGNCC 1 cut(s) 276
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 22
CviJI RGCY 5 cut(s) 103, 167, 243, 277, 397
CviKI_1 RGCY 5 cut(s) 103, 167, 243, 277, 397
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 236
DpnI GATC 2 cut(s) 202, 362
DpnII GATC 2 cut(s) 200, 360
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 354
FaiI YATR 2 cut(s) 37, 216
FblI GTMKAC 2 cut(s) 308, 325
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 303
GluI GCNGC 1 cut(s) 303
GsaI CCCAGC 1 cut(s) 118
HaeIII GGCC 1 cut(s) 277
HapII CCGG 2 cut(s) 27, 164
HgaI GACGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 1 cut(s) 388
HpaII CCGG 2 cut(s) 27, 164
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 121, 309, 326
Hpy188I TCNGA 2 cut(s) 174, 205
Hpy188III TCNNGA 1 cut(s) 27
Hpy8I GTNNAC 3 cut(s) 121, 309, 326
HpyCH4V TGCA 6 cut(s) 60, 72, 84, 150, 260, 302
HpyF3I CTNAG 1 cut(s) 236
Kpn2I TCCGGA 1 cut(s) 26
Kzo9I GATC 2 cut(s) 200, 360
LpnPI CCDG 6 cut(s) 40, 100, 100, 153, 177, 297
Lsp1109I GCAGC 1 cut(s) 314
LweI GCATC 1 cut(s) 269
MalI GATC 2 cut(s) 202, 362
MboI GATC 2 cut(s) 200, 360
MboII GAAGA 5 cut(s) 152, 241, 273, 285, 360
MfeI CAATTG 1 cut(s) 151
MluCI AATT 1 cut(s) 151
MlyI GAGTC 1 cut(s) 382
MnlI CCTC 8 cut(s) 10, 120, 274, 282, 289, 354, 382, 388
Mph1103I ATGCAT 1 cut(s) 262
MroI TCCGGA 1 cut(s) 26
MroXI GAANNNNTTC 1 cut(s) 244
MseI TTAA 1 cut(s) 287
MspI CCGG 2 cut(s) 27, 164
MunI CAATTG 1 cut(s) 151
NdeII GATC 2 cut(s) 200, 360
NmeAIII GCCGAG 2 cut(s) 253, 359
NsiI ATGCAT 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 244
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PsiI TTATAA 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 114
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 1 cut(s) 304
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 2 cut(s) 200, 360
Sau96I GGNCC 1 cut(s) 276
ScaI AGTACT 1 cut(s) 23
SchI GAGTC 1 cut(s) 382
SetI ASST 3 cut(s) 189, 380, 399
SfaNI GCATC 1 cut(s) 269
SfcI CTRYAG 2 cut(s) 300, 309
Sse9I AATT 1 cut(s) 151
SsiI CCGC 1 cut(s) 124
TaqI TCGA 2 cut(s) 133, 248
TasI AATT 1 cut(s) 151
TatI WGTACW 1 cut(s) 21
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 2 cut(s) 304, 341
TseI GCWGC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 2 cut(s) 304, 341
XmiI GTMKAC 2 cut(s) 308, 325
XmnI GAANNNNTTC 1 cut(s) 244
ZrmI AGTACT 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.