MD05G1207500.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010449
Physical Location & Seq
Forward (+)
33822704 .. 33823135
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1207500.v1.1.491

Sequence Viewer

Length: 432 bp
ATGCTTTGTAGGATGGTATTGGTGCCGATGAAGAAGAAGATTGGGAAGGTGCCCGTTTATCTGAACGTGTACGATCTTACTCCGATCAATGGCTACGCCTATTGGCTCGGCCTCGGAGTCTACCATTCCGGCGTCCAAGTACATGGCGTTGAATATGCCTTTGGAGCTTATGACCATGCAACGACTGGGATTTTCGAAGTGGAACCGAAGCAATGCCCCAACTTTGTGTTCAGAAAATCAATTTTGATCGGAAGAACGGATTTGGATCCGAAGGAGGTCCGTGCTTTTATGGAGAAACTAACAGAGGAATACTCCGGGAACCCGTACCATCTTATCACCAAGAACTGCAACCATGGCGCGGTACAACAAGGTTGCCCCGAAGCCTCTGCTGAAAGACTGATCGGATCATATTTATATATATTTTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

144

Amino Acids

16.17

Weight (kDa)

7.68

Isoelectric Point (pI)

22.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 18 - 118 8.5e-38 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 49
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 359
AciI CCGC 1 cut(s) 359
AclWI GGATC 3 cut(s) 260, 273, 412
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 4 cut(s) 71, 141, 326, 363
AfiI CCNNNNNNNGG 2 cut(s) 89, 358
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 212, 244
AluBI AGCT 1 cut(s) 167
AluI AGCT 1 cut(s) 167
AlwI GGATC 3 cut(s) 260, 273, 412
AoxI GGCC 1 cut(s) 109
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 195
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 54
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 22, 49
BccI CCATC 2 cut(s) 7, 336
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BcnI CCSGG 1 cut(s) 316
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 5 cut(s) 24, 51, 204, 267, 320
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 195
BmuI ACTGGG 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu14I TTCGAA 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 316
BsaBI GATNNNNATC 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 112, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 358
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 218
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 112, 352
BseGI GGATG 1 cut(s) 18
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 89, 358
BseMI GCAATG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 1 cut(s) 111
BshNI GGYRCC 2 cut(s) 22, 49
BsiSI CCGG 2 cut(s) 129, 315
BslI CCNNNNNNNGG 2 cut(s) 89, 358
BsnI GGCC 1 cut(s) 111
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 6 cut(s) 73, 84, 246, 265, 399, 404
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 1 cut(s) 359
BspANI GGCC 1 cut(s) 111
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 5 cut(s) 24, 51, 204, 267, 320
BspPI GGATC 3 cut(s) 260, 273, 412
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 2 cut(s) 22, 49
BsrDI GCAATG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 112, 352
BssMI GATC 6 cut(s) 73, 84, 246, 265, 399, 404
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 352
BstACI GRCGYC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 18
BstFNI CGCG 1 cut(s) 359
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 6 cut(s) 76, 87, 249, 268, 402, 407
BstMBI GATC 6 cut(s) 73, 84, 246, 265, 399, 404
BstMWI GCNNNNNNNGC 2 cut(s) 164, 354
BstSCI CCNGG 1 cut(s) 314
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 1 cut(s) 359
BstX2I RGATCY 1 cut(s) 265
BstXI CCANNNNNNTGG 1 cut(s) 143
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 1 cut(s) 18
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 277
CseI GACGC 1 cut(s) 121
Csp6I GTAC 4 cut(s) 70, 140, 325, 362
CviAII CATG 3 cut(s) 143, 176, 353
CviJI RGCY 5 cut(s) 93, 106, 111, 167, 383
CviKI_1 RGCY 5 cut(s) 93, 106, 111, 167, 383
CviQI GTAC 4 cut(s) 70, 140, 325, 362
DpnI GATC 6 cut(s) 75, 86, 248, 267, 401, 406
DpnII GATC 6 cut(s) 73, 84, 246, 265, 399, 404
Eco130I CCWWGG 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 3 cut(s) 146, 179, 356
FatI CATG 3 cut(s) 142, 175, 352
FblI GTMKAC 1 cut(s) 120
FokI GGATG 1 cut(s) 25
GlaI GCGC 1 cut(s) 358
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 129, 315
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 359
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 3 cut(s) 146, 179, 356
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HinfI GANTC 1 cut(s) 117
HpaII CCGG 2 cut(s) 129, 315
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 70, 121
Hpy188I TCNGA 7 cut(s) 63, 84, 116, 233, 251, 270, 404
Hpy8I GTNNAC 2 cut(s) 70, 121
HpyAV CCTTC 2 cut(s) 40, 265
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 179, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 354
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 3 cut(s) 146, 179, 356
HspAI GCGC 1 cut(s) 357
Kzo9I GATC 6 cut(s) 73, 84, 246, 265, 399, 404
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 3 cut(s) 142, 171, 328
MaeII ACGT 1 cut(s) 66
MalI GATC 6 cut(s) 75, 86, 248, 267, 401, 406
MboI GATC 6 cut(s) 73, 84, 246, 265, 399, 404
MboII GAAGA 4 cut(s) 43, 46, 49, 264
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 1 cut(s) 240
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 4 cut(s) 122, 268, 298, 394
MseI TTAA 1 cut(s) 430
MspI CCGG 2 cut(s) 129, 315
MspR9I CCNGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 164, 354
NciI CCSGG 1 cut(s) 316
NcoI CCATGG 1 cut(s) 352
NdeII GATC 6 cut(s) 73, 84, 246, 265, 399, 404
NlaIII CATG 3 cut(s) 146, 179, 356
NlaIV GGNNCC 5 cut(s) 24, 51, 204, 267, 320
NmeAIII GCCGAG 1 cut(s) 87
NspV TTCGAA 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 314
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PspN4I GGNNCC 5 cut(s) 24, 51, 204, 267, 320
PspPI GGNCC 1 cut(s) 277
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 4 cut(s) 71, 141, 326, 363
RsaNI GTAC 4 cut(s) 70, 140, 325, 362
SaqAI TTAA 1 cut(s) 430
Sau3AI GATC 6 cut(s) 73, 84, 246, 265, 399, 404
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 1 cut(s) 54
SetI ASST 5 cut(s) 51, 69, 169, 279, 373
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 1 cut(s) 277
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 352
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 195
TasI AATT 1 cut(s) 240
TatI WGTACW 1 cut(s) 139
Tru1I TTAA 1 cut(s) 430
Tru9I TTAA 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 44
TspGWI ACGGA 2 cut(s) 269, 272
VpaK11BI GGWCC 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.