pycom15g03420

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010449
Physical Location & Seq
Reverse (-)
2103187 .. 2104663
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03420.1

Sequence Viewer

Length: 690 bp
ATGCTTTGTAGGATGGTATTGGTGCCGAGGAAGAAGAAGATTGGGAAGGTGCCCGTTTATCTGAACGTGTACGATCTTACTCCGATCAATGGCTACGCGTATTGGCTCGGCCTCGGAGTCTACCATTCCGGCGTCCAAGTACATGGCGTTGAATATGCCTTTGGAGCTCATGACCATGCAACGACTGGGATTTTCGAAGTGGAACCGAAGCAATGCCCCGGCTTTGTGTTCAGAAAATCAATTTTGATCGGAGGAACGGAGATCGATCCGAAGGAGGTCCGTGCATTTATGGAGAAACTAGCAGAGGAATACTCCGGGAACACGTACCATCTTATCACCAAGAACTGCAACCACTTCTGTAATGATGTGTGCACCAGATTGACGGGGAAGCCTATTCCTCGCTGGGTCAACCGCCTCGCTCGGCTTGGTTTCTTCTGCAATTGTGTTCTTCCGGCTGGTCTTAACGAAACTAAGGTTCGACAAGTAAGATCAGAGGGGGTTTGTAACGGAGACAAGAAGAAACTGAGAAGCCATTCGACTAATTGTGCATCCTCAAATCCTCGGTCCTCTTCATTAAAATCTTCCACTGCAGAGTCTGCAGGCAGAAGTAGTAGACAAAGACACTGCGTTCCTCCGTCTCAATCTTTGGTCCATTCTTCCTCAACCTCGGCGGTTACTCTAAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

230

Amino Acids

25.34

Weight (kDa)

9.54

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 18 - 152 3.8e-55 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 49
AccI GTMKAC 2 cut(s) 120, 613
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 412, 671
AclWI GGATC 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 3 cut(s) 71, 141, 326
AfiI CCNNNNNNNGG 1 cut(s) 89
AflIII ACRYGT 3 cut(s) 66, 96, 321
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 2 cut(s) 167, 685
AluI AGCT 2 cut(s) 167, 685
Alw21I GWGCWC 2 cut(s) 169, 374
Alw26I GTCTC 2 cut(s) 504, 642
Alw44I GTGCAC 1 cut(s) 370
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 596
AoxI GGCC 1 cut(s) 109
ApaLI GTGCAC 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 532
AspS9I GGNCC 3 cut(s) 277, 564, 649
AsuC2I CCSGG 2 cut(s) 219, 316
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 195
AvaII GGWCC 3 cut(s) 277, 564, 649
BaeGI GKGCMC 2 cut(s) 54, 374
BanI GGYRCC 2 cut(s) 22, 49
BanII GRGCYC 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 169, 374
BccI CCATC 2 cut(s) 7, 336
BcnI CCSGG 2 cut(s) 219, 316
BcoDI GTCTC 2 cut(s) 504, 642
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 2 cut(s) 588, 597
Bme1390I CCNGG 2 cut(s) 219, 316
Bme18I GGWCC 3 cut(s) 277, 564, 649
BmgT120I GGNCC 3 cut(s) 277, 564, 649
BmiI GGNNCC 3 cut(s) 24, 51, 204
BmrFI CCNGG 2 cut(s) 219, 316
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu14I TTCGAA 1 cut(s) 195
BpuMI CCSGG 2 cut(s) 219, 316
Bsa29I ATCGAT 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 26, 112, 217, 560, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 218
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 5 cut(s) 26, 112, 217, 560, 666
BseGI GGATG 2 cut(s) 18, 548
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 2 cut(s) 54, 374
BseYI CCCAGC 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 1 cut(s) 111
BshNI GGYRCC 2 cut(s) 22, 49
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 169, 374
BsiSI CCGG 4 cut(s) 129, 219, 315, 452
BslI CCNNNNNNNGG 1 cut(s) 89
BsmAI GTCTC 2 cut(s) 504, 642
BsmBI CGTCTC 1 cut(s) 642
BsnI GGCC 1 cut(s) 111
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 54, 169, 374
Bsp143I GATC 6 cut(s) 73, 84, 246, 261, 265, 488
BspACI CCGC 2 cut(s) 412, 671
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 516
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 3 cut(s) 24, 51, 204
BspMAI CTGCAG 2 cut(s) 592, 601
BspPI GGATC 1 cut(s) 260
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 2 cut(s) 22, 49
BsrDI GCAATG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 5 cut(s) 26, 112, 217, 560, 666
BssMI GATC 6 cut(s) 73, 84, 246, 261, 265, 488
BssNI GRCGYC 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 574
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 324
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 601
BstDEI CTNAG 2 cut(s) 471, 524
BstF5I GGATG 2 cut(s) 18, 548
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 6 cut(s) 76, 87, 249, 264, 268, 491
BstMAI GTCTC 2 cut(s) 504, 642
BstMBI GATC 6 cut(s) 73, 84, 246, 261, 265, 488
BstMWI GCNNNNNNNGC 2 cut(s) 164, 596
BstSCI CCNGG 2 cut(s) 217, 314
BstSFI CTRYAG 2 cut(s) 588, 597
BstSLI GKGCMC 2 cut(s) 54, 374
BstUI CGCG 1 cut(s) 98
BstXI CCANNNNNNTGG 1 cut(s) 143
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 1 cut(s) 111
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 2 cut(s) 18, 548
BtsI GCAGTG 2 cut(s) 585, 622
BtsIMutI CAGTG 2 cut(s) 585, 622
Cac8I GCNNGC 1 cut(s) 601
CaiI CAGNNNCTG 1 cut(s) 596
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 277, 564, 649
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 121
Csp6I GTAC 3 cut(s) 70, 140, 325
CviAII CATG 3 cut(s) 143, 170, 176
CviQI GTAC 3 cut(s) 70, 140, 325
DdeI CTNAG 2 cut(s) 471, 524
DpnI GATC 6 cut(s) 75, 86, 248, 263, 267, 490
DpnII GATC 6 cut(s) 73, 84, 246, 261, 265, 488
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
Ecl136II GAGCTC 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 277, 564, 649
Eco53kI GAGCTC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 169
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 3 cut(s) 146, 173, 179
FaiI YATR 5 cut(s) 144, 156, 171, 177, 290
FatI CATG 3 cut(s) 142, 169, 175
FblI GTMKAC 2 cut(s) 120, 613
FokI GGATG 2 cut(s) 25, 535
FriOI GRGCYC 1 cut(s) 169
FspBI CTAG 1 cut(s) 299
GsaI CCCAGC 1 cut(s) 406
HaeIII GGCC 1 cut(s) 111
HapII CCGG 4 cut(s) 129, 219, 315, 452
HgaI GACGC 1 cut(s) 121
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 3 cut(s) 146, 173, 179
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 2 cut(s) 117, 593
HpaII CCGG 4 cut(s) 129, 219, 315, 452
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 5 cut(s) 70, 121, 372, 409, 614
Hpy188I TCNGA 7 cut(s) 63, 84, 116, 233, 251, 270, 493
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 5 cut(s) 70, 121, 372, 409, 614
HpyAV CCTTC 2 cut(s) 40, 265
HpyCH4IV ACGT 2 cut(s) 66, 323
HpyCH4V TGCA 8 cut(s) 179, 284, 348, 372, 438, 548, 590, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 596
HpyF3I CTNAG 2 cut(s) 471, 524
HpySE526I ACGT 2 cut(s) 66, 323
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 3 cut(s) 146, 173, 179
Kzo9I GATC 6 cut(s) 73, 84, 246, 261, 265, 488
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 9 cut(s) 142, 171, 232, 328, 388, 388, 441, 465, 585
LweI GCATC 1 cut(s) 557
MaeI CTAG 1 cut(s) 299
MaeII ACGT 2 cut(s) 66, 323
MaeIII GTNAC 2 cut(s) 503, 673
MalI GATC 6 cut(s) 75, 86, 248, 263, 267, 490
MboI GATC 6 cut(s) 73, 84, 246, 261, 265, 488
MboII GAAGA 9 cut(s) 43, 46, 49, 424, 440, 529, 561, 573, 648
MfeI CAATTG 1 cut(s) 439
MhlI GDGCHC 3 cut(s) 54, 169, 374
MluCI AATT 3 cut(s) 240, 439, 541
MluI ACGCGT 1 cut(s) 96
MlyI GAGTC 2 cut(s) 126, 602
MroXI GAANNNNTTC 1 cut(s) 532
MseI TTAA 2 cut(s) 462, 575
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 4 cut(s) 129, 219, 315, 452
MspR9I CCNGG 2 cut(s) 219, 316
MunI CAATTG 1 cut(s) 439
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 164, 596
NciI CCSGG 2 cut(s) 219, 316
NdeII GATC 6 cut(s) 73, 84, 246, 261, 265, 488
NlaIII CATG 3 cut(s) 146, 173, 179
NlaIV GGNNCC 3 cut(s) 24, 51, 204
NmeAIII GCCGAG 4 cut(s) 51, 87, 400, 647
NspV TTCGAA 1 cut(s) 195
PagI TCATGA 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 532
PfoI TCCNGGA 1 cut(s) 314
PleI GAGTC 2 cut(s) 125, 601
PpsI GAGTC 2 cut(s) 125, 601
Ppu21I YACGTR 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 402
PspN4I GGNNCC 3 cut(s) 24, 51, 204
PspPI GGNCC 3 cut(s) 277, 564, 649
PstI CTGCAG 2 cut(s) 592, 601
PstNI CAGNNNCTG 1 cut(s) 596
RsaI GTAC 3 cut(s) 71, 141, 326
RsaNI GTAC 3 cut(s) 70, 140, 325
RseI CAYNNNNRTG 1 cut(s) 174
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 2 cut(s) 462, 575
Sau3AI GATC 6 cut(s) 73, 84, 246, 261, 265, 488
Sau96I GGNCC 3 cut(s) 277, 564, 649
SchI GAGTC 2 cut(s) 126, 602
ScrFI CCNGG 2 cut(s) 219, 316
SduI GDGCHC 3 cut(s) 54, 169, 374
SetI ASST 8 cut(s) 51, 69, 169, 279, 326, 477, 668, 687
SfaNI GCATC 1 cut(s) 557
SfcI CTRYAG 2 cut(s) 588, 597
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 3 cut(s) 277, 564, 649
SmiMI CAYNNNNRTG 1 cut(s) 174
Sse9I AATT 3 cut(s) 240, 439, 541
SsiI CCGC 2 cut(s) 412, 671
SspMI CTAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 217, 314
TaiI ACGT 2 cut(s) 69, 326
TaqI TCGA 4 cut(s) 195, 264, 478, 536
TaqII GACCGA 1 cut(s) 552
TasI AATT 3 cut(s) 240, 439, 541
TatI WGTACW 1 cut(s) 139
Tru1I TTAA 2 cut(s) 462, 575
Tru9I TTAA 2 cut(s) 462, 575
TscAI CASTG 2 cut(s) 592, 629
TspDTI ATGAA 1 cut(s) 561
TspGWI ACGGA 4 cut(s) 269, 272, 522, 624
TspRI CASTG 2 cut(s) 592, 629
VneI GTGCAC 1 cut(s) 370
VpaK11BI GGWCC 3 cut(s) 277, 564, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmiI GTMKAC 2 cut(s) 120, 613
XmnI GAANNNNTTC 1 cut(s) 532
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.