AT5G25360

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009786
Physical Location & Seq
Reverse (-)
8799710 .. 8802753
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25360.3

Sequence Viewer

Length: 510 bp
ATGCATCTCCGTGAAATCATTCCCTCTTGGATCTACCAGTTATTTGGTTGCATGGGAGGTTGTTTTGGATGCTGCAATAAACCACCGCTTATAGTTGCAGTGGATGAGCCGTCTAAAGGGTTAAGGATTCAAGGTCGTTTAGTGAAGAAGCCAAGCGTATCAGAGGACTTCTGGAGCACAAGCACCTGCGAGATGGATAATAGTACACTTCAGTCACAGAGAAGCATGTCTTCTATTAGCTTCACTAACAATACTTCTACTTCTGCAAGTACAAGCAACCCCACAGAATTTGTAAACCACGGATTGAACCTCTGGAACCAAACTAGGCAACAGTGGCTTGCAAATGGAACTTCTCAAAAAAAGGCCAAAGTTCGAGAACCTACAATAAGCTGGAATGCAACATATGAGAGCTTGTTGGGTATGAACAAGCGGTTTTCTCGTCCAATACCACTCCCTGAAATGGTGGATTTTTTGGTGGATGTGTGGGAGCAAGAAGGCTTGTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

169

Amino Acids

19.13

Weight (kDa)

6.72

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 108 - 216 2e-23 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AciI CCGC 2 cut(s) 86, 430
AclWI GGATC 1 cut(s) 38
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 194
AfaI GTAC 2 cut(s) 205, 271
AfiI CCNNNNNNNGG 2 cut(s) 116, 460
AgsI TTSAA 2 cut(s) 131, 307
AluBI AGCT 3 cut(s) 240, 390, 411
AluI AGCT 3 cut(s) 240, 390, 411
Alw21I GWGCWC 1 cut(s) 179
AlwI GGATC 1 cut(s) 38
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 59
BccI CCATC 1 cut(s) 187
BceAI ACGGC 1 cut(s) 94
BfaI CTAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 317
BmsI GCATC 2 cut(s) 13, 59
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 298
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 460
Bse1I ACTGG 1 cut(s) 37
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 3 cut(s) 74, 109, 484
BseLI CCNNNNNNNGG 2 cut(s) 116, 460
BseNI ACTGG 1 cut(s) 37
BseXI GCAGC 1 cut(s) 59
BshFI GGCC 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 179
BslI CCNNNNNNNGG 2 cut(s) 116, 460
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 1 cut(s) 30
BspACI CCGC 2 cut(s) 86, 430
BspANI GGCC 1 cut(s) 365
BspLI GGNNCC 1 cut(s) 317
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 1 cut(s) 38
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 74, 109, 484
BstKTI GATC 1 cut(s) 33
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNSI RCATGY 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 1 cut(s) 365
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 3 cut(s) 74, 109, 484
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 338
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 339
Csp6I GTAC 2 cut(s) 204, 270
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 2 cut(s) 52, 226
CviJI RGCY 8 cut(s) 109, 151, 240, 337, 365, 390, 411, 498
CviKI_1 RGCY 8 cut(s) 109, 151, 240, 337, 365, 390, 411, 498
CviQI GTAC 2 cut(s) 204, 270
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 55, 229
FaiI YATR 7 cut(s) 53, 92, 227, 403, 405, 422, 504
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FatI CATG 2 cut(s) 51, 225
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 3 cut(s) 81, 116, 491
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 1 cut(s) 324
GluI GCNGC 1 cut(s) 73
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 1 cut(s) 365
Hin1II CATG 2 cut(s) 55, 229
HinfI GANTC 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 206, 295
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 172, 313, 374
Hpy8I GTNNAC 2 cut(s) 206, 295
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4V TGCA 7 cut(s) 4, 51, 75, 98, 266, 341, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
Hsp92II CATG 2 cut(s) 55, 229
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 2 cut(s) 174, 487
LpnPI CCDG 6 cut(s) 50, 157, 199, 298, 376, 468
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 2 cut(s) 13, 59
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 213
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 2 cut(s) 157, 222
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 1 cut(s) 287
MnlI CCTC 4 cut(s) 34, 50, 157, 320
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 122
MslI CAYNNNNRTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeI CATATG 1 cut(s) 403
NdeII GATC 1 cut(s) 30
NlaIII CATG 2 cut(s) 55, 229
NlaIV GGNNCC 1 cut(s) 317
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 229
PaqCI CACCTGC 1 cut(s) 194
PctI GAATGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 317
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 2 cut(s) 205, 271
RsaNI GTAC 2 cut(s) 204, 270
RseI CAYNNNNRTG 1 cut(s) 9
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 1 cut(s) 30
SduI GDGCHC 1 cut(s) 179
SetI ASST 8 cut(s) 61, 136, 188, 242, 312, 382, 392, 413
SfaNI GCATC 2 cut(s) 13, 59
SmiMI CAYNNNNRTG 1 cut(s) 9
Sse9I AATT 1 cut(s) 287
SsiI CCGC 2 cut(s) 86, 430
SspMI CTAG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 373
TasI AATT 1 cut(s) 287
TatI WGTACW 2 cut(s) 203, 269
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TscAI CASTG 2 cut(s) 105, 338
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 437
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 105, 338
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.