Rw2G002950

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009786
Physical Location & Seq
Reverse (-)
2779093 .. 2781339
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002950.1

Sequence Viewer

Length: 549 bp
ATGGTGTCGCTCAACGGTTCCCTATCTGCTTGGATCCATCACTTGCTTGCTTGCATGGGTGGTTGTTTTGGATGTTGCACGAAACCCACACCGATTATTGCTGTCGACGAGCCTTCAAAGGGATTGAAAATTCAAGGGCGGTCGGTGAAGAAACCTGGCATATCGGATGATTTTTGGAGCAGCAGCACGTACGATTTGGACAACAGCGTTGTGCAGTCCCAGAGAAGCATCTCATCCATCAGCACCTTACACCAGACCCTCCTCCATGGCAGCGGCGCTGGTAGCACGAGCAGCCAACCTGACTTTGTAAATCAAGGCAAGTTTCCATTGCGTCTTGTTCTCTGGAACCAAACCAGGCTTGAGTGGATTGGAAATGGCAAGCCTAAGGATGAAACTCTAAGAAGTCGGGAAAACAGATTAAGTTGGAATTCAACTTACGAAAGATTGGTTGGACCCAAACAGAGACAGCCTTTTCCCCAGCGCATTCCTCTGTCTGAAATGATAGAATTTCTAGTGGAAGTATGGGAGCAGGAGGGGCTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

182

Amino Acids

20.39

Weight (kDa)

7.7

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 62 - 182 3.6e-17 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AciI CCGC 2 cut(s) 139, 273
AclWI GGATC 2 cut(s) 28, 41
AcsI RAATTY 3 cut(s) 129, 427, 506
AfaI GTAC 2 cut(s) 191, 542
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 4 cut(s) 117, 127, 134, 432
AjnI CCWGG 2 cut(s) 154, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 28, 41
ApeKI GCWGC 4 cut(s) 180, 183, 270, 291
ApoI RAATTY 3 cut(s) 129, 427, 506
AspLEI GCGC 2 cut(s) 278, 483
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 384
BamHI GGATCC 1 cut(s) 33
BauI CACGAG 1 cut(s) 286
BbvI GCAGC 4 cut(s) 192, 195, 282, 303
BccI CCATC 2 cut(s) 45, 245
BciT130I CCWGG 2 cut(s) 156, 355
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 1 cut(s) 512
BfoI RGCGCY 1 cut(s) 279
BisI GCNGC 5 cut(s) 181, 184, 271, 274, 292
BlsI GCNGC 5 cut(s) 182, 185, 272, 275, 293
Bme1390I CCNGG 2 cut(s) 156, 355
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 4 cut(s) 19, 35, 347, 454
BmrFI CCNGG 2 cut(s) 156, 355
BmsI GCATC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 1 cut(s) 524
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse21I CCTNAGG 1 cut(s) 384
Bse3DI GCAATG 1 cut(s) 326
BseBI CCWGG 2 cut(s) 156, 355
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 4 cut(s) 77, 172, 233, 394
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 4 cut(s) 192, 195, 282, 303
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 233
Bsh1285I CGRYCG 1 cut(s) 143
BsiEI CGRYCG 1 cut(s) 143
BsiWI CGTACG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 483
Bsp143I GATC 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 2 cut(s) 139, 273
BspLI GGNNCC 4 cut(s) 19, 35, 347, 454
BspPI GGATC 2 cut(s) 28, 41
BsrDI GCAATG 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 156, 355
Bst4CI ACNGT 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 48, 52, 380
BstDEI CTNAG 2 cut(s) 384, 398
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 4 cut(s) 77, 172, 233, 394
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 2 cut(s) 278, 483
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 282, 291, 535
BstNI CCWGG 2 cut(s) 156, 355
BstSCI CCNGG 2 cut(s) 154, 353
BstV1I GCAGC 4 cut(s) 192, 195, 282, 303
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 4 cut(s) 77, 172, 233, 394
Cac8I GCNNGC 3 cut(s) 48, 52, 380
CfoI GCGC 2 cut(s) 278, 483
Cfr13I GGNCC 1 cut(s) 452
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 190, 541
CviAII CATG 2 cut(s) 55, 266
CviJI RGCY 6 cut(s) 112, 294, 358, 382, 469, 538
CviKI_1 RGCY 6 cut(s) 112, 294, 358, 382, 469, 538
CviQI GTAC 2 cut(s) 190, 541
DdeI CTNAG 2 cut(s) 384, 398
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 452
Eco81I CCTNAGG 1 cut(s) 384
EcoRI GAATTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 154, 353
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 2 cut(s) 58, 269
FaiI YATR 4 cut(s) 56, 161, 267, 523
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 54, 265
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 5 cut(s) 181, 184, 271, 274, 292
FokI GGATG 4 cut(s) 84, 179, 220, 401
Fsp4HI GCNGC 5 cut(s) 181, 184, 271, 274, 292
FspBI CTAG 1 cut(s) 512
GlaI GCGC 2 cut(s) 277, 482
GluI GCNGC 5 cut(s) 181, 184, 271, 274, 292
GsaI CCCAGC 1 cut(s) 481
HaeII RGCGCY 1 cut(s) 279
HgaI GACGC 1 cut(s) 320
HhaI GCGC 2 cut(s) 278, 483
Hin1II CATG 2 cut(s) 58, 269
Hin6I GCGC 2 cut(s) 276, 481
HinP1I GCGC 2 cut(s) 276, 481
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 166, 496
Hpy188III TCNNGA 2 cut(s) 343, 407
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 123
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 54, 78, 214
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 291, 535
HpyF3I CTNAG 2 cut(s) 384, 398
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 2 cut(s) 58, 269
HspAI GCGC 2 cut(s) 276, 481
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 177, 526
Lsp1109I GCAGC 4 cut(s) 192, 195, 282, 303
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 512
MaeII ACGT 1 cut(s) 188
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 33
MluCI AATT 3 cut(s) 129, 427, 506
MmeI TCCRAC 2 cut(s) 404, 430
MnlI CCTC 4 cut(s) 269, 272, 498, 526
MseI TTAA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 156, 355
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 2 cut(s) 156, 355
MwoI GCNNNNNNNGC 3 cut(s) 282, 291, 535
NcoI CCATGG 1 cut(s) 265
NdeII GATC 1 cut(s) 33
NlaIII CATG 2 cut(s) 58, 269
NlaIV GGNNCC 4 cut(s) 19, 35, 347, 454
PctI GAATGC 1 cut(s) 483
Pfl23II CGTACG 1 cut(s) 189
PkrI GCNGC 5 cut(s) 182, 185, 272, 275, 293
Ppu21I YACGTR 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 154, 353
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 2 cut(s) 154, 353
PspLI CGTACG 1 cut(s) 189
PspN4I GGNNCC 4 cut(s) 19, 35, 347, 454
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 2 cut(s) 191, 542
RsaNI GTAC 2 cut(s) 190, 541
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 5 cut(s) 181, 184, 271, 274, 292
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 156, 355
SetI ASST 4 cut(s) 157, 191, 248, 301
SfaNI GCATC 1 cut(s) 237
SinI GGWCC 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 3 cut(s) 129, 427, 506
SsiI CCGC 2 cut(s) 139, 273
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 2 cut(s) 154, 353
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 191
TaqI TCGA 1 cut(s) 105
TasI AATT 3 cut(s) 129, 427, 506
TauI GCSGC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TseI GCWGC 4 cut(s) 180, 183, 270, 291
TspDTI ATGAA 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 3 cut(s) 129, 427, 506
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.