FvH4_1g03140

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009786
Physical Location & Seq
Reverse (-)
1770052 .. 1772925
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03140.t1

Sequence Viewer

Length: 534 bp
ATGGTGACGCTCAACGGTTCCATATCGGCTTGGATCCATCACTTGCTTGCTTGCATGGGTGGTTGTTTTGGATGCTGCACTAAACCCACACCGATTATTGCTGTCGACGAGCCTTCAAAGGGATTGAAAATCCAAGGGCGGGCGGTGAAGAAACCTGGTTTATCGGATGATTTTTGGAGCAGCAGCACGTATGACTTGGACAACAGCGCTTTGCAGTCCCAGAGAAGCATCTCATCCATCAGCACATTACACCAGACACTTCTCCAGGGGAGCAGCATTGGTAGCACGAGCAGCCAACCTGACTTTGTAAATCAAGGTCTTGTTCTCTGGAACCAAACTAGGCTTGAGTGGACTGGAAGTGGCAAGCCTAAGAATGAAGCTCTAAGAAGTCGGGAAAGCAGATTAAGTTGGAATTCAACTTATGAGAGATTGGTTGGACCCAAACAGAGACAGCCTTTTCCCCAGCGTGTTCCTTTGTCTGAAATGATAGAATTCCTAGTCGAAGTATGGGAGCAGGAAGGGTTGTACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

178

Amino Acids

19.69

Weight (kDa)

6.82

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 62 - 177 1.4e-20 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AciI CCGC 2 cut(s) 139, 143
AclWI GGATC 2 cut(s) 28, 41
AcsI RAATTY 2 cut(s) 412, 491
AfaI GTAC 1 cut(s) 527
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 119, 139
AgsI TTSAA 3 cut(s) 117, 127, 417
AjnI CCWGG 2 cut(s) 154, 264
AluBI AGCT 1 cut(s) 380
AluI AGCT 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 442
AlwI GGATC 2 cut(s) 28, 41
Aor51HI AGCGCT 1 cut(s) 208
ApeKI GCWGC 5 cut(s) 75, 180, 183, 273, 291
ApoI RAATTY 2 cut(s) 412, 491
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 16, 157
AvaII GGWCC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 33
BauI CACGAG 1 cut(s) 286
BbvI GCAGC 5 cut(s) 62, 192, 195, 285, 303
BccI CCATC 2 cut(s) 45, 245
BciT130I CCWGG 2 cut(s) 156, 266
BcoDI GTCTC 1 cut(s) 442
BfaI CTAG 2 cut(s) 339, 497
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 5 cut(s) 76, 181, 184, 274, 292
BlsI GCNGC 5 cut(s) 77, 182, 185, 275, 293
Bme1390I CCNGG 2 cut(s) 156, 266
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 4 cut(s) 19, 35, 332, 439
BmrFI CCNGG 2 cut(s) 156, 266
BmsI GCATC 2 cut(s) 62, 237
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 133, 265
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 139
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 2 cut(s) 156, 266
BseDI CCNNGG 2 cut(s) 133, 265
BseGI GGATG 3 cut(s) 77, 172, 233
BseLI CCNNNNNNNGG 2 cut(s) 119, 139
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 5 cut(s) 62, 192, 195, 285, 303
BseYI CCCAGC 1 cut(s) 462
BsgI GTGCAG 1 cut(s) 61
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 119, 139
BsmAI GTCTC 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 202
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 2 cut(s) 139, 143
BspLI GGNNCC 4 cut(s) 19, 35, 332, 439
BspPI GGATC 2 cut(s) 28, 41
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 133, 265
BssMI GATC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 133
Bst2BI CACGAG 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 156, 266
Bst4CI ACNGT 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 4 cut(s) 48, 52, 141, 365
BstDEI CTNAG 2 cut(s) 369, 383
BstF5I GGATG 3 cut(s) 77, 172, 233
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 282, 291
BstNI CCWGG 2 cut(s) 156, 266
BstSCI CCNGG 2 cut(s) 154, 264
BstV1I GCAGC 5 cut(s) 62, 192, 195, 285, 303
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BtsCI GGATG 3 cut(s) 77, 172, 233
Cac8I GCNNGC 4 cut(s) 48, 52, 141, 365
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 1 cut(s) 16
CsiI ACCWGGT 1 cut(s) 154
Csp6I GTAC 1 cut(s) 526
CviAII CATG 1 cut(s) 55
CviJI RGCY 7 cut(s) 29, 112, 294, 343, 367, 380, 454
CviKI_1 RGCY 7 cut(s) 29, 112, 294, 343, 367, 380, 454
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 2 cut(s) 369, 383
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 437
Eco47III AGCGCT 1 cut(s) 208
EcoRI GAATTC 2 cut(s) 412, 491
EcoRII CCWGG 2 cut(s) 154, 264
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 1 cut(s) 58
FaiI YATR 5 cut(s) 23, 56, 192, 423, 508
FaqI GGGAC 1 cut(s) 202
FatI CATG 1 cut(s) 54
FauI CCCGC 1 cut(s) 132
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 5 cut(s) 76, 181, 184, 274, 292
FokI GGATG 3 cut(s) 84, 179, 220
Fsp4HI GCNGC 5 cut(s) 76, 181, 184, 274, 292
FspBI CTAG 2 cut(s) 339, 497
GlaI GCGC 1 cut(s) 208
GluI GCNGC 5 cut(s) 76, 181, 184, 274, 292
GsaI CCCAGC 1 cut(s) 466
GsuI CTGGAG 1 cut(s) 248
HaeII RGCGCY 1 cut(s) 210
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 209
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HphI GGTGA 2 cut(s) 16, 157
Hpy166II GTNNAC 2 cut(s) 106, 351
Hpy188I TCNGA 2 cut(s) 166, 481
Hpy188III TCNNGA 2 cut(s) 328, 392
Hpy8I GTNNAC 2 cut(s) 106, 351
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 2 cut(s) 123, 512
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 54, 78, 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 291
HpyF3I CTNAG 2 cut(s) 369, 383
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 207
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 3 cut(s) 177, 270, 511
Lsp1109I GCAGC 5 cut(s) 62, 192, 195, 285, 303
LweI GCATC 2 cut(s) 62, 237
MabI ACCWGGT 1 cut(s) 154
MaeI CTAG 2 cut(s) 339, 497
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 4
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 33
MluCI AATT 2 cut(s) 412, 491
MmeI TCCRAC 2 cut(s) 389, 415
MseI TTAA 2 cut(s) 404, 532
MspR9I CCNGG 2 cut(s) 156, 266
MvaI CCWGG 2 cut(s) 156, 266
MwoI GCNNNNNNNGC 2 cut(s) 282, 291
NdeII GATC 1 cut(s) 33
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 4 cut(s) 19, 35, 332, 439
NmuCI GTSAC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 77, 182, 185, 275, 293
Ppu21I YACGTR 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 154, 264
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 2 cut(s) 154, 264
PspN4I GGNNCC 4 cut(s) 19, 35, 332, 439
PspPI GGNCC 1 cut(s) 437
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 2 cut(s) 404, 532
SatI GCNGC 5 cut(s) 76, 181, 184, 274, 292
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 156, 266
SetI ASST 5 cut(s) 157, 191, 301, 319, 382
SexAI ACCWGGT 1 cut(s) 154
SfaNI GCATC 2 cut(s) 62, 237
SinI GGWCC 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 2 cut(s) 412, 491
SsiI CCGC 2 cut(s) 139, 143
SspMI CTAG 2 cut(s) 339, 497
StyD4I CCNGG 2 cut(s) 154, 264
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 191
TaqI TCGA 2 cut(s) 105, 501
TasI AATT 2 cut(s) 412, 491
Tru1I TTAA 2 cut(s) 404, 532
Tru9I TTAA 2 cut(s) 404, 532
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 5 cut(s) 75, 180, 183, 273, 291
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 2 cut(s) 412, 491
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 2 cut(s) 339, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.