Rh2DG037200

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009786
Physical Location & Seq
Reverse (-)
2563681 .. 2565920
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG037200.1

Sequence Viewer

Length: 534 bp
ATGGTGTCGCTCAACGGTTCCCTATCTGCTTGGATCCATCACTTGCTTGCTTGCATGGGTGGTTGTTTTGGATGTTGCACGAAACCCACACCGATTATTGCTGTCGACGAGCCTTCAAAGGGATTGAAAATTCAAGGGCGGTCGGTGAAGAGACCTGGCATATCGGATGATTTTTGGAGCAGCAGCACGTATGATTTGGACAACAGCGCTGTGCAGTCCCAGAGAAGCGTCTCATCCATCAGCACCTTACACCAGACCCTCCTCCATGGCAGCGGCGCTGGTAGCACGAGCAGCCAACCTGACTTTGTAAATCAAGGTCTTGTTCTCTGGAACCAAACCAGGCTTGAGTGGATTGGAAATGGCAAGCCTAAGGATGAAACTCTAAGAAGTCGGGAAAATAGATTAAGTTGGAATTCAACTTACGAAAGATTGGTTGGACCCAAACAGAGACAGCCTTTTCCCCAGCGCATTCCTCTGTCTGAAATGATAGAATTTCTAGTGGAAGTATGGGAGCAGGAGGGGCTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

177

Amino Acids

19.74

Weight (kDa)

6.42

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 62 - 177 7.8e-21 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AciI CCGC 2 cut(s) 139, 273
AclWI GGATC 2 cut(s) 28, 41
AcsI RAATTY 3 cut(s) 129, 412, 491
AfaI GTAC 1 cut(s) 527
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 4 cut(s) 117, 127, 134, 417
AjnI CCWGG 2 cut(s) 154, 338
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
Alw26I GTCTC 3 cut(s) 145, 235, 442
AlwI GGATC 2 cut(s) 28, 41
Aor51HI AGCGCT 1 cut(s) 208
ApeKI GCWGC 4 cut(s) 180, 183, 270, 291
ApoI RAATTY 3 cut(s) 129, 412, 491
AspLEI GCGC 3 cut(s) 209, 278, 468
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 437
AxyI CCTNAGG 1 cut(s) 369
BamHI GGATCC 1 cut(s) 33
BauI CACGAG 1 cut(s) 286
BbvI GCAGC 4 cut(s) 192, 195, 282, 303
BccI CCATC 2 cut(s) 45, 245
BciT130I CCWGG 2 cut(s) 156, 340
BcoDI GTCTC 3 cut(s) 145, 235, 442
BfaI CTAG 1 cut(s) 497
BfoI RGCGCY 2 cut(s) 210, 279
BisI GCNGC 5 cut(s) 181, 184, 271, 274, 292
BlsI GCNGC 5 cut(s) 182, 185, 272, 275, 293
Bme1390I CCNGG 2 cut(s) 156, 340
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 4 cut(s) 19, 35, 332, 439
BmrFI CCNGG 2 cut(s) 156, 340
BpuEI CTTGAG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse21I CCTNAGG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 156, 340
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 4 cut(s) 77, 172, 233, 379
BseLI CCNNNNNNNGG 1 cut(s) 119
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 4 cut(s) 192, 195, 282, 303
BseYI CCCAGC 1 cut(s) 462
BsgI GTGCAG 1 cut(s) 233
Bsh1285I CGRYCG 1 cut(s) 143
BsiEI CGRYCG 1 cut(s) 143
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 3 cut(s) 145, 235, 442
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 468
Bso31I GGTCTC 1 cut(s) 145
Bsp143I GATC 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 2 cut(s) 139, 273
BspLI GGNNCC 4 cut(s) 19, 35, 332, 439
BspPI GGATC 2 cut(s) 28, 41
BspTNI GGTCTC 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 156, 340
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 48, 52, 365
BstDEI CTNAG 2 cut(s) 369, 383
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 4 cut(s) 77, 172, 233, 379
BstH2I RGCGCY 2 cut(s) 210, 279
BstHHI GCGC 3 cut(s) 209, 278, 468
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 3 cut(s) 145, 235, 442
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 282, 291, 520
BstNI CCWGG 2 cut(s) 156, 340
BstSCI CCNGG 2 cut(s) 154, 338
BstV1I GCAGC 4 cut(s) 192, 195, 282, 303
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 4 cut(s) 77, 172, 233, 379
Cac8I GCNNGC 3 cut(s) 48, 52, 365
CfoI GCGC 3 cut(s) 209, 278, 468
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 526
CviAII CATG 2 cut(s) 55, 266
CviJI RGCY 6 cut(s) 112, 294, 343, 367, 454, 523
CviKI_1 RGCY 6 cut(s) 112, 294, 343, 367, 454, 523
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 2 cut(s) 369, 383
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 437
Eco47III AGCGCT 1 cut(s) 208
Eco81I CCTNAGG 1 cut(s) 369
EcoRI GAATTC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 154, 338
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 2 cut(s) 58, 269
FaiI YATR 5 cut(s) 56, 161, 192, 267, 508
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 54, 265
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 5 cut(s) 181, 184, 271, 274, 292
FokI GGATG 4 cut(s) 84, 179, 220, 386
Fsp4HI GCNGC 5 cut(s) 181, 184, 271, 274, 292
FspBI CTAG 1 cut(s) 497
GlaI GCGC 3 cut(s) 208, 277, 467
GluI GCNGC 5 cut(s) 181, 184, 271, 274, 292
GsaI CCCAGC 1 cut(s) 466
HaeII RGCGCY 2 cut(s) 210, 279
HgaI GACGC 1 cut(s) 217
HhaI GCGC 3 cut(s) 209, 278, 468
Hin1II CATG 2 cut(s) 58, 269
Hin6I GCGC 3 cut(s) 207, 276, 466
HinP1I GCGC 3 cut(s) 207, 276, 466
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 166, 481
Hpy188III TCNNGA 2 cut(s) 328, 392
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 123
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 54, 78, 214
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 291, 520
HpyF3I CTNAG 2 cut(s) 369, 383
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 2 cut(s) 58, 269
HspAI GCGC 3 cut(s) 207, 276, 466
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 177, 511
Lsp1109I GCAGC 4 cut(s) 192, 195, 282, 303
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 188
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 33
MluCI AATT 3 cut(s) 129, 412, 491
MmeI TCCRAC 2 cut(s) 389, 415
MnlI CCTC 4 cut(s) 269, 272, 483, 511
MseI TTAA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 156, 340
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 2 cut(s) 156, 340
MwoI GCNNNNNNNGC 3 cut(s) 282, 291, 520
NcoI CCATGG 1 cut(s) 265
NdeII GATC 1 cut(s) 33
NlaIII CATG 2 cut(s) 58, 269
NlaIV GGNNCC 4 cut(s) 19, 35, 332, 439
PctI GAATGC 1 cut(s) 468
PkrI GCNGC 5 cut(s) 182, 185, 272, 275, 293
Ppu21I YACGTR 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 154, 338
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 2 cut(s) 154, 338
PspN4I GGNNCC 4 cut(s) 19, 35, 332, 439
PspPI GGNCC 1 cut(s) 437
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 5 cut(s) 181, 184, 271, 274, 292
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 156, 340
SetI ASST 5 cut(s) 157, 191, 248, 301, 319
SinI GGWCC 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 129, 412, 491
SsiI CCGC 2 cut(s) 139, 273
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 154, 338
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 191
TaqI TCGA 1 cut(s) 105
TasI AATT 3 cut(s) 129, 412, 491
TauI GCSGC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TseI GCWGC 4 cut(s) 180, 183, 270, 291
TspDTI ATGAA 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 3 cut(s) 129, 412, 491
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.