AT5G25950

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017074
Physical Location & Seq
Reverse (-)
9057793 .. 9059963
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25950.1

Sequence Viewer

Length: 1299 bp
ATGAAGTTTCAAGATTCAAAAGCCTATATCCTAAGAACTCCAAGACAAACAGCCACAATGAGATTTTTCTTAATCTTTGTGATTTTGTGTGGTTTTTACAATGAAGCATATGGGAAAGGTTCTTTAGATATTGATCTGAAATTGAAGGCTCTTAATAAACCTGCGTTGAAGACCATCAAGAGCGAAGATGGAGATATAATAGATTGTATAGATATCTATAAACAACATGCCTTTGATCATCCCGCTTTAAAAAACCACAAAATTCAGATGAAACCGAGTGTGAAATTTGGTACAAAGAAGACTACTATACCAAACAACGGTTCTTCTGAACACATAAAATCTCAGATTTGGTCTAAATCCGGAAAATGTCCAATGGGGACCATACCGGTTCGTAGAGTTAGTAGAGAAGACATAAGCAGAGCCTCTTCACCGTCTCATTTTGGGAGGAAAACTCCTCACAAATACAGCTTTCTAGACAACGCACTTCAACATAAGGGTAATTTCAATATAACACCTGCAAAAATAAACGAAGCCCAACCAAGGTTAAGATCGGAGGCATTTATTGTGGCCCTAGGGTTCAATTTTGTCGGAGCTCAATCTGATATAAATATTTGGAACCCTCCGAGGGTTGAAGCTACCGACTACAGTACTGCCCAGATCTGGCTGGTTGGTGGTCTCTCAGAGAATTTTGAGAGCGTTGAAGGTGGTTGGATGGTGAATCCAGCTGTTTTCGGAGACTCACGTACCCGTCTCTTCATCTCCTGGACTACAGATGGATACACTAAAACAGGGTGCATCAATCTCTTATGCGCGGGTTTTGTGCAAACAAGTAAAAAATTTGCCCTTGGTGCAACCGTTGAACCTGTTTCGAGTTCCTCTAGTACGCAATATCACATCACCGTTAGCATATTCCTTGATCCAAACAGTGGGAACTGGTGGTTGACTTGCGAAAACAACGTGTTGGGTTATTGGCCGGGTACACTATTCAACTACCTAAAACACAGCGCCACAGCTGTGCAGTGGGGTGGAGAAGTACATAGCCCTAACGTAGTATTGAAGAAGCCACACACGACAACCGCAATGGGGAGTGGACAATGGGCATCTTACATCTGGGCCGAAGCTTGTTTCCACACAAACCTTAGGATCAAGGACTATTCCATGCAGCTCAAATACCCCCAGTTTCTATCCGAGTATGCTGATGAGTACAACTGTTATTCTACACTGCTCCACCGGAAAACATATATGTCAGAGCCCCACTTTTACTTCGGAGGACCTGGCCGGAACTCTCGTTGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

432

Amino Acids

48.66

Weight (kDa)

9.24

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 58 - 153 5.1e-36 Neprosin activation peptide
Neprosin PF03080 213 - 425 4.6e-72 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 523
Acc36I ACCTGC 2 cut(s) 169, 523
AccB7I CCANNNNNTGG 1 cut(s) 926
AccII CGCG 1 cut(s) 812
AccIII TCCGGA 1 cut(s) 359
AciI CCGC 3 cut(s) 243, 812, 1077
AclWI GGATC 2 cut(s) 911, 1151
AcoI YGGCCR 2 cut(s) 971, 1276
AcsI RAATTY 4 cut(s) 261, 284, 685, 836
AfaI GTAC 7 cut(s) 292, 649, 745, 883, 979, 1035, 1205
AfiI CCNNNNNNNGG 6 cut(s) 317, 540, 625, 660, 926, 1083
AflIII ACRYGT 1 cut(s) 957
AgeI ACCGGT 1 cut(s) 385
AjnI CCWGG 2 cut(s) 761, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AleI CACNNNNGTG 1 cut(s) 1013
AluBI AGCT 7 cut(s) 468, 593, 635, 725, 1013, 1121, 1165
AluI AGCT 7 cut(s) 468, 593, 635, 725, 1013, 1121, 1165
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 4 cut(s) 438, 680, 729, 755
AlwI GGATC 2 cut(s) 911, 1151
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 4 cut(s) 567, 971, 1113, 1276
ApeKI GCWGC 1 cut(s) 1162
ApoI RAATTY 4 cut(s) 261, 284, 685, 836
AsiGI ACCGGT 1 cut(s) 385
AspA2I CCTAGG 1 cut(s) 571
AspLEI GCGC 2 cut(s) 812, 1007
AspS9I GGNCC 4 cut(s) 378, 568, 1113, 1271
AsuC2I CCSGG 1 cut(s) 975
AsuHPI GGTGA 3 cut(s) 420, 727, 889
AvaII GGWCC 2 cut(s) 378, 1271
AvrII CCTAGG 1 cut(s) 571
AxyI CCTNAGG 1 cut(s) 1139
BaeI ACNNNNGTAYC 2 cut(s) 727, 760
BanII GRGCYC 2 cut(s) 595, 1254
BbsI GAAGAC 3 cut(s) 176, 305, 414
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 1 cut(s) 1174
BccI CCATC 4 cut(s) 182, 182, 706, 767
BciT130I CCWGG 2 cut(s) 763, 1275
BciVI GTATCC 1 cut(s) 770
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 975
BcoDI GTCTC 4 cut(s) 438, 680, 729, 755
BfaI CTAG 3 cut(s) 473, 572, 879
BfmI CTRYAG 2 cut(s) 643, 768
BfoI RGCGCY 1 cut(s) 1008
BfuAI ACCTGC 2 cut(s) 169, 523
BfuI GTATCC 1 cut(s) 770
BglII AGATCT 1 cut(s) 657
BisI GCNGC 1 cut(s) 1163
BlnI CCTAGG 1 cut(s) 571
BlsI GCNGC 1 cut(s) 1164
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 3 cut(s) 763, 975, 1275
Bme18I GGWCC 2 cut(s) 378, 1271
BmgT120I GGNCC 4 cut(s) 378, 568, 1113, 1271
BmiI GGNNCC 2 cut(s) 379, 617
BmrFI CCNGG 3 cut(s) 763, 975, 1275
BmrI ACTGGG 1 cut(s) 1171
BmsI GCATC 2 cut(s) 804, 1109
BmuI ACTGGG 1 cut(s) 1171
BpiI GAAGAC 3 cut(s) 176, 305, 414
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpuMI CCSGG 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 4 cut(s) 539, 571, 623, 844
BsaWI WCCGGW 3 cut(s) 359, 385, 1230
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 540, 625, 660, 926, 1083
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 2 cut(s) 938, 1177
Bse21I CCTNAGG 1 cut(s) 1139
Bse3DI GCAATG 1 cut(s) 1086
Bse8I GATNNNNATC 1 cut(s) 132
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 2 cut(s) 763, 1275
BseDI CCNNGG 4 cut(s) 539, 571, 623, 844
BseGI GGATG 2 cut(s) 238, 717
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 6 cut(s) 317, 540, 625, 660, 926, 1083
BseMI GCAATG 1 cut(s) 1086
BseMII CTCAG 2 cut(s) 356, 693
BseNI ACTGG 2 cut(s) 938, 1177
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 1174
BsgI GTGCAG 1 cut(s) 1037
Bsh1236I CGCG 1 cut(s) 812
BshFI GGCC 4 cut(s) 569, 973, 1115, 1278
BshTI ACCGGT 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 5 cut(s) 360, 386, 974, 1231, 1279
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 6 cut(s) 317, 540, 625, 660, 926, 1083
BsmAI GTCTC 4 cut(s) 438, 680, 729, 755
BsmBI CGTCTC 2 cut(s) 438, 755
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 4 cut(s) 569, 973, 1115, 1278
Bso31I GGTCTC 1 cut(s) 680
Bsp1286I GDGCHC 2 cut(s) 595, 1254
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
BspACI CCGC 3 cut(s) 243, 812, 1077
BspANI GGCC 4 cut(s) 569, 973, 1115, 1278
BspCNI CTCAG 2 cut(s) 355, 692
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 812
BspLI GGNNCC 2 cut(s) 379, 617
BspMI ACCTGC 2 cut(s) 169, 523
BspPI GGATC 2 cut(s) 911, 1151
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 1086
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 2 cut(s) 938, 1177
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 539, 571, 623, 844
BssMI GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
BssT1I CCWWGG 3 cut(s) 539, 571, 844
Bst2UI CCWGG 2 cut(s) 763, 1275
Bst4CI ACNGT 7 cut(s) 320, 432, 647, 856, 901, 926, 1211
Bst6I CTCTTC 2 cut(s) 430, 758
BstBAI YACGTR 1 cut(s) 743
BstDEI CTNAG 4 cut(s) 32, 342, 679, 1139
BstF5I GGATG 2 cut(s) 238, 717
BstFNI CGCG 1 cut(s) 812
BstH2I RGCGCY 1 cut(s) 1008
BstHHI GCGC 2 cut(s) 812, 1007
BstKTI GATC 6 cut(s) 136, 238, 551, 660, 919, 1146
BstMAI GTCTC 4 cut(s) 438, 680, 729, 755
BstMBI GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
BstMWI GCNNNNNNNGC 1 cut(s) 848
BstNI CCWGG 2 cut(s) 763, 1275
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 761, 973, 1273
BstSFI CTRYAG 2 cut(s) 643, 768
BstUI CGCG 1 cut(s) 812
BstV1I GCAGC 1 cut(s) 1174
BstV2I GAAGAC 3 cut(s) 176, 305, 414
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
Bsu36I CCTNAGG 1 cut(s) 1139
BsuI GTATCC 1 cut(s) 770
BsuRI GGCC 4 cut(s) 569, 973, 1115, 1278
BtsCI GGATG 2 cut(s) 238, 717
BtsI GCAGTG 2 cut(s) 1025, 1220
BtsIMutI CAGTG 3 cut(s) 931, 1025, 1220
BveI ACCTGC 2 cut(s) 169, 523
CfoI GCGC 2 cut(s) 812, 1007
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 378, 568, 1113, 1271
Csp6I GTAC 7 cut(s) 291, 648, 744, 882, 978, 1034, 1204
CspAI ACCGGT 1 cut(s) 385
CviAII CATG 2 cut(s) 227, 1159
CviQI GTAC 7 cut(s) 291, 648, 744, 882, 978, 1034, 1204
DdeI CTNAG 4 cut(s) 32, 342, 679, 1139
DpnI GATC 6 cut(s) 135, 237, 550, 659, 918, 1145
DpnII GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
DraI TTTAAA 1 cut(s) 249
EaeI YGGCCR 2 cut(s) 971, 1276
Eam1104I CTCTTC 2 cut(s) 430, 758
EarI CTCTTC 2 cut(s) 430, 758
Ecl136II GAGCTC 1 cut(s) 593
Eco130I CCWWGG 3 cut(s) 539, 571, 844
Eco24I GRGCYC 2 cut(s) 595, 1254
Eco31I GGTCTC 1 cut(s) 680
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 378, 1271
Eco53kI GAGCTC 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 1139
EcoICRI GAGCTC 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 1271
EcoRII CCWGG 2 cut(s) 761, 1273
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 3 cut(s) 539, 571, 844
EcoT38I GRGCYC 2 cut(s) 595, 1254
ErhI CCWWGG 3 cut(s) 539, 571, 844
Esp3I CGTCTC 2 cut(s) 438, 755
FaeI CATG 2 cut(s) 230, 1162
FaqI GGGAC 1 cut(s) 391
FatI CATG 2 cut(s) 226, 1158
FauI CCCGC 2 cut(s) 250, 805
FauNDI CATATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 1163
FokI GGATG 2 cut(s) 225, 724
FriOI GRGCYC 2 cut(s) 595, 1254
Fsp4HI GCNGC 1 cut(s) 1163
FspBI CTAG 3 cut(s) 473, 572, 879
GlaI GCGC 2 cut(s) 811, 1006
GluI GCNGC 1 cut(s) 1163
HaeII RGCGCY 1 cut(s) 1008
HaeIII GGCC 4 cut(s) 569, 973, 1115, 1278
HapII CCGG 5 cut(s) 360, 386, 974, 1231, 1279
HhaI GCGC 2 cut(s) 812, 1007
Hin1II CATG 2 cut(s) 230, 1162
Hin6I GCGC 2 cut(s) 810, 1005
HinP1I GCGC 2 cut(s) 810, 1005
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HindIII AAGCTT 1 cut(s) 1119
HinfI GANTC 3 cut(s) 14, 718, 737
HpaII CCGG 5 cut(s) 360, 386, 974, 1231, 1279
HphI GGTGA 3 cut(s) 420, 727, 889
Hpy166II GTNNAC 3 cut(s) 942, 980, 1091
Hpy188III TCNNGA 4 cut(s) 11, 178, 360, 473
Hpy8I GTNNAC 3 cut(s) 942, 980, 1091
HpyAV CCTTC 2 cut(s) 139, 695
HpyCH4III ACNGT 7 cut(s) 320, 432, 647, 856, 901, 926, 1211
HpyCH4IV ACGT 3 cut(s) 742, 957, 1047
HpyCH4V TGCA 6 cut(s) 518, 795, 823, 851, 1018, 1162
HpyF10VI GCNNNNNNNGC 1 cut(s) 848
HpyF3I CTNAG 4 cut(s) 32, 342, 679, 1139
HpySE526I ACGT 3 cut(s) 742, 957, 1047
Hsp92II CATG 2 cut(s) 230, 1162
HspAI GCGC 2 cut(s) 810, 1005
Kpn2I TCCGGA 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
LmnI GCTCC 2 cut(s) 590, 1230
Lsp1109I GCAGC 1 cut(s) 1174
LweI GCATC 2 cut(s) 804, 1109
MaeI CTAG 3 cut(s) 473, 572, 879
MaeII ACGT 3 cut(s) 742, 957, 1047
MalI GATC 6 cut(s) 135, 237, 550, 659, 918, 1145
MboI GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
MboII GAAGA 8 cut(s) 181, 197, 310, 315, 417, 419, 745, 1069
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 2 cut(s) 595, 1254
MluCI AATT 7 cut(s) 140, 261, 284, 499, 580, 685, 836
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 2 cut(s) 568, 689
MnlI CCTC 8 cut(s) 433, 438, 465, 547, 618, 630, 886, 1262
MroI TCCGGA 1 cut(s) 359
MseI TTAA 5 cut(s) 71, 153, 248, 545, 1297
MslI CAYNNNNRTG 1 cut(s) 1013
MspA1I CMGCKG 2 cut(s) 725, 1013
MspI CCGG 5 cut(s) 360, 386, 974, 1231, 1279
MspR9I CCNGG 3 cut(s) 763, 975, 1275
MvaI CCWGG 2 cut(s) 763, 1275
MvnI CGCG 1 cut(s) 812
MwoI GCNNNNNNNGC 1 cut(s) 848
NciI CCSGG 1 cut(s) 975
NdeI CATATG 1 cut(s) 109
NdeII GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
NlaIII CATG 2 cut(s) 230, 1162
NlaIV GGNNCC 2 cut(s) 379, 617
NspI RCATGY 1 cut(s) 230
OliI CACNNNNGTG 1 cut(s) 1013
PaqCI CACCTGC 1 cut(s) 523
PfeI GAWTC 2 cut(s) 14, 718
PflMI CCANNNNNTGG 1 cut(s) 926
PfoI TCCNGGA 1 cut(s) 761
PinAI ACCGGT 1 cut(s) 385
PkrI GCNGC 1 cut(s) 1164
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
Ppu21I YACGTR 1 cut(s) 743
PpuMI RGGWCCY 1 cut(s) 1271
Psp124BI GAGCTC 1 cut(s) 595
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 2 cut(s) 761, 1273
PspGI CCWGG 2 cut(s) 761, 1273
PspN4I GGNNCC 2 cut(s) 379, 617
PspPI GGNCC 4 cut(s) 378, 568, 1113, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PsuI RGATCY 1 cut(s) 657
PvuII CAGCTG 2 cut(s) 725, 1013
RsaI GTAC 7 cut(s) 292, 649, 745, 883, 979, 1035, 1205
RsaNI GTAC 7 cut(s) 291, 648, 744, 882, 978, 1034, 1204
RseI CAYNNNNRTG 1 cut(s) 1013
SacI GAGCTC 1 cut(s) 595
SaqAI TTAA 5 cut(s) 71, 153, 248, 545, 1297
SatI GCNGC 1 cut(s) 1163
Sau3AI GATC 6 cut(s) 133, 235, 548, 657, 916, 1143
Sau96I GGNCC 4 cut(s) 378, 568, 1113, 1271
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 3 cut(s) 763, 975, 1275
SduI GDGCHC 2 cut(s) 595, 1254
SfaNI GCATC 2 cut(s) 804, 1109
SfcI CTRYAG 2 cut(s) 643, 768
SinI GGWCC 2 cut(s) 378, 1271
SmiMI CAYNNNNRTG 1 cut(s) 1013
Sse9I AATT 7 cut(s) 140, 261, 284, 499, 580, 685, 836
SsiI CCGC 3 cut(s) 243, 812, 1077
SspI AATATT 1 cut(s) 610
SspMI CTAG 3 cut(s) 473, 572, 879
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 3 cut(s) 761, 973, 1273
StyI CCWWGG 3 cut(s) 539, 571, 844
TaaI ACNGT 7 cut(s) 320, 432, 647, 856, 901, 926, 1211
TaiI ACGT 3 cut(s) 745, 960, 1050
TaqI TCGA 1 cut(s) 869
TasI AATT 7 cut(s) 140, 261, 284, 499, 580, 685, 836
TatI WGTACW 3 cut(s) 647, 1033, 1203
TfiI GAWTC 2 cut(s) 14, 718
Tru1I TTAA 5 cut(s) 71, 153, 248, 545, 1297
Tru9I TTAA 5 cut(s) 71, 153, 248, 545, 1297
TscAI CASTG 3 cut(s) 931, 1025, 1227
TseI GCWGC 1 cut(s) 1162
TspDTI ATGAA 4 cut(s) 17, 117, 284, 745
TspRI CASTG 3 cut(s) 931, 1025, 1227
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 2 cut(s) 378, 1271
XapI RAATTY 4 cut(s) 261, 284, 685, 836
XbaI TCTAGA 1 cut(s) 472
XceI RCATGY 1 cut(s) 230
XmaJI CCTAGG 1 cut(s) 571
XspI CTAG 3 cut(s) 473, 572, 879
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.