Rroxscaffold_7G00158560

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017074
Physical Location & Seq
Forward (+)
1950786 .. 1951596
811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158560.1

Sequence Viewer

Length: 555 bp
ATGGACCTGGAAATGCCTTTAAACCCAAAGTTGTATGGTGATTCAGCTACTCGACTTTTCGTGTATTGGACAGTAAGTACTAGAAGAGCATACCCCACATTGAATTTTTACTATTTGTTACTATATATATATATATATATATATATATATATATATATATATATATATATAATGTCCTGGTCTCACCAAGCCGGTCCTAAATCTATTTCCTTTGATCTAACTTGCTCAGGATTTGTGCAAACTAATAAAGACATAGCCCTTGGTATGCCCCTAGGACCTGTATCAAGTGCAGCATCAAGGTTTGGACGCTTTTCTTTGGTCTATATGCATTCTCTTCACATTGATCCAATGTCTAATGTGACCATTCAATTTCAATGTTTTGGGATCAAAACACAGGCAATTGGTGGCTACGTTCGGGTCAAAACGTCCCGGTACCCAGAATGGGTAGGCACTTTTGTCGATGAAGCGTATTGCACTGAACCACGCTCAGTCGCAGCCCAAGAACCTACATTTTACTTTGGAGGACCTGGTCGATATCCTCCTAGTTGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

184

Amino Acids

21.08

Weight (kDa)

8.59

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 57 - 98 1.3e-06 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AclWI GGATC 2 cut(s) 338, 392
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 2 cut(s) 79, 434
AfiI CCNNNNNNNGG 1 cut(s) 442
AgsI TTSAA 3 cut(s) 103, 368, 374
AjnI CCWGG 3 cut(s) 6, 176, 526
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 2 cut(s) 338, 392
ApeKI GCWGC 2 cut(s) 290, 494
ApoI RAATTY 1 cut(s) 103
Asp718I GGTACC 1 cut(s) 432
AspA2I CCTAGG 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 4, 194, 275, 524
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 2 cut(s) 50, 176
AvaII GGWCC 4 cut(s) 4, 194, 275, 524
AvrII CCTAGG 1 cut(s) 271
BanI GGYRCC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 302, 506
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 3 cut(s) 8, 178, 528
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 3 cut(s) 81, 272, 543
BisI GCNGC 2 cut(s) 291, 495
BlnI CCTAGG 1 cut(s) 271
BlsI GCNGC 2 cut(s) 292, 496
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 4 cut(s) 8, 178, 430, 528
Bme18I GGWCC 4 cut(s) 4, 194, 275, 524
BmgT120I GGNCC 4 cut(s) 4, 194, 275, 524
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 4 cut(s) 8, 178, 430, 528
BmsI GCATC 1 cut(s) 302
Bpu10I CCTNAGC 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 430
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 259, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 191
BseBI CCWGG 3 cut(s) 8, 178, 528
BseDI CCNNGG 2 cut(s) 259, 271
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMII CTCAG 2 cut(s) 240, 501
BseXI GCAGC 2 cut(s) 302, 506
BsgI GTGCAG 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 432
BsiSI CCGG 2 cut(s) 192, 430
BslFI GGGAC 2 cut(s) 412, 534
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 412, 534
BsmI GAATGC 1 cut(s) 328
Bso31I GGTCTC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 214, 343, 384
BspCNI CTCAG 2 cut(s) 239, 500
BspLI GGNNCC 1 cut(s) 434
BspPI GGATC 2 cut(s) 338, 392
BspQI GCTCTTC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 259, 271
BssMI GATC 3 cut(s) 214, 343, 384
BssT1I CCWWGG 2 cut(s) 259, 271
Bst2UI CCWGG 3 cut(s) 8, 178, 528
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 2 cut(s) 79, 339
BstDEI CTNAG 2 cut(s) 226, 487
BstKTI GATC 3 cut(s) 217, 346, 387
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 214, 343, 384
BstNI CCWGG 3 cut(s) 8, 178, 528
BstSCI CCNGG 4 cut(s) 6, 176, 428, 526
BstV1I GCAGC 2 cut(s) 302, 506
BtsIMutI CAGTG 1 cut(s) 474
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 4 cut(s) 4, 194, 275, 524
CseI GACGC 1 cut(s) 315
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 78, 433
CviJI RGCY 5 cut(s) 47, 191, 257, 408, 497
CviKI_1 RGCY 5 cut(s) 47, 191, 257, 408, 497
CviQI GTAC 2 cut(s) 78, 433
DdeI CTNAG 2 cut(s) 226, 487
DpnI GATC 3 cut(s) 216, 345, 386
DpnII GATC 3 cut(s) 214, 343, 384
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 79, 339
EarI CTCTTC 2 cut(s) 79, 339
Eco130I CCWWGG 2 cut(s) 259, 271
Eco31I GGTCTC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 536
Eco47I GGWCC 4 cut(s) 4, 194, 275, 524
EcoO109I RGGNCCY 2 cut(s) 275, 524
EcoRII CCWGG 3 cut(s) 6, 176, 526
EcoRV GATATC 1 cut(s) 536
EcoT14I CCWWGG 2 cut(s) 259, 271
EcoT22I ATGCAT 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 259, 271
FaqI GGGAC 2 cut(s) 412, 534
Fnu4HI GCNGC 2 cut(s) 291, 495
Fsp4HI GCNGC 2 cut(s) 291, 495
FspBI CTAG 3 cut(s) 81, 272, 543
GluI GCNGC 2 cut(s) 291, 495
HapII CCGG 2 cut(s) 192, 430
HgaI GACGC 1 cut(s) 315
HinfI GANTC 1 cut(s) 41
HpaII CCGG 2 cut(s) 192, 430
HphI GGTGA 2 cut(s) 50, 176
Hpy188III TCNNGA 1 cut(s) 228
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 411, 425
HpyCH4V TGCA 4 cut(s) 238, 290, 328, 474
HpyF3I CTNAG 2 cut(s) 226, 487
HpySE526I ACGT 2 cut(s) 411, 425
KpnI GGTACC 1 cut(s) 436
Kzo9I GATC 3 cut(s) 214, 343, 384
LguI GCTCTTC 1 cut(s) 79
Lsp1109I GCAGC 2 cut(s) 302, 506
LweI GCATC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 3 cut(s) 81, 272, 543
MaeII ACGT 2 cut(s) 411, 425
MaeIII GTNAC 2 cut(s) 117, 358
MalI GATC 3 cut(s) 216, 345, 386
MboI GATC 3 cut(s) 214, 343, 384
MboII GAAGA 2 cut(s) 96, 326
MfeI CAATTG 1 cut(s) 399
MluCI AATT 3 cut(s) 103, 368, 399
MnlI CCTC 2 cut(s) 515, 549
Mph1103I ATGCAT 1 cut(s) 330
MseI TTAA 1 cut(s) 20
MspI CCGG 2 cut(s) 192, 430
MspR9I CCNGG 4 cut(s) 8, 178, 430, 528
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 3 cut(s) 8, 178, 528
NciI CCSGG 1 cut(s) 430
NdeII GATC 3 cut(s) 214, 343, 384
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 79
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 41
PflFI GACNNNGTC 1 cut(s) 528
PkrI GCNGC 2 cut(s) 292, 496
PpuMI RGGWCCY 2 cut(s) 275, 524
Psp5II RGGWCCY 2 cut(s) 275, 524
Psp6I CCWGG 3 cut(s) 6, 176, 526
PspGI CCWGG 3 cut(s) 6, 176, 526
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 4 cut(s) 4, 194, 275, 524
PspPPI RGGWCCY 2 cut(s) 275, 524
PsyI GACNNNGTC 1 cut(s) 528
RsaI GTAC 2 cut(s) 79, 434
RsaNI GTAC 2 cut(s) 78, 433
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 2 cut(s) 291, 495
Sau3AI GATC 3 cut(s) 214, 343, 384
Sau96I GGNCC 4 cut(s) 4, 194, 275, 524
ScaI AGTACT 1 cut(s) 79
ScrFI CCNGG 4 cut(s) 8, 178, 430, 528
SetI ASST 8 cut(s) 9, 49, 280, 302, 414, 428, 508, 529
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 1 cut(s) 302
SinI GGWCC 4 cut(s) 4, 194, 275, 524
Sse9I AATT 3 cut(s) 103, 368, 399
SspMI CTAG 3 cut(s) 81, 272, 543
StyD4I CCNGG 4 cut(s) 6, 176, 428, 526
StyI CCWWGG 2 cut(s) 259, 271
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 2 cut(s) 414, 428
TaqI TCGA 3 cut(s) 52, 459, 532
TasI AATT 3 cut(s) 103, 368, 399
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 481
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 290, 494
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 477
TspRI CASTG 1 cut(s) 481
Tth111I GACNNNGTC 1 cut(s) 528
VpaK11BI GGWCC 4 cut(s) 4, 194, 275, 524
XapI RAATTY 1 cut(s) 103
XmaJI CCTAGG 1 cut(s) 271
XspI CTAG 3 cut(s) 81, 272, 543
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.