Rh6DG494700

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0017074
Physical Location & Seq
Reverse (-)
65342712 .. 65343248
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG494700.1

Sequence Viewer

Length: 432 bp
ATGCCCCTAGGACCTGTATCAAGTGGAGGAGGATCCCAATATCAAACCACTTTCAGCATCAACAAGGATCAAAACACAGCCAATTGGTGGCTACGTTTGGGTCAAAACGTCCCGGTAGGATATTGGCCACCAACTCTCTTCTCCTATCTGAAACAAGGTCCTGCAACATTGGTTGAATGGGGTGGGGAGGTGTATAGCTCCAAGGTGAAGGAGACTCGTCCACACACTGGGACTGGCAAAGATGGAAGTGCTTGTTACGTGAAACAAGTGAGAATCGTGGATTGTTCACTGCAACTAAAGTACCCAGAATGGGTAGGCACTTTTGTCGATGAAGCGTATTGCTACTCTGCACTGAACTACGCTCAGTCGTTTGCCCAAGAACCTACATTTTACTTTGGAGGACCTGGTCGATATCCTCCTAGTTGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

143

Amino Acids

15.85

Weight (kDa)

7.61

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 2 - 135 1.8e-34 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 87, 227
AclWI GGATC 3 cut(s) 27, 40, 75
AcoI YGGCCR 1 cut(s) 125
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 87, 227, 310
AgsI TTSAA 1 cut(s) 176
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 3 cut(s) 27, 40, 75
AoxI GGCC 1 cut(s) 125
AspA2I CCTAGG 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 11, 158, 401
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 3 cut(s) 11, 158, 401
AvrII CCTAGG 1 cut(s) 7
BaeI ACNNNNGTAYC 2 cut(s) 284, 317
BalI TGGCCA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 32
BccI CCATC 1 cut(s) 236
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 405
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 8, 420
BlnI CCTAGG 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 113, 405
Bme18I GGWCC 3 cut(s) 11, 158, 401
BmgT120I GGNCC 3 cut(s) 11, 158, 401
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 2 cut(s) 113, 405
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 66
BmuI ACTGGG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 259
BsaJI CCNNGG 2 cut(s) 7, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 227, 310
Bse1I ACTGG 2 cut(s) 232, 238
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 7, 201
BseLI CCNNNNNNNGG 3 cut(s) 87, 227, 310
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 232, 238
BseRI GAGGAG 1 cut(s) 42
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 3 cut(s) 95, 244, 411
BslI CCNNNNNNNGG 3 cut(s) 87, 227, 310
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 3 cut(s) 95, 244, 411
BsnI GGCC 1 cut(s) 127
Bsp143I GATC 2 cut(s) 32, 67
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 3 cut(s) 27, 40, 75
BsrI ACTGG 2 cut(s) 232, 238
BssECI CCNNGG 2 cut(s) 7, 201
BssMI GATC 2 cut(s) 32, 67
BssT1I CCWWGG 2 cut(s) 7, 201
Bst2UI CCWGG 1 cut(s) 405
Bst6I CTCTTC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 259
BstDEI CTNAG 1 cut(s) 363
BstKTI GATC 2 cut(s) 35, 70
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 2 cut(s) 32, 67
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 2 cut(s) 111, 403
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 3 cut(s) 225, 287, 350
Cfr13I GGNCC 3 cut(s) 11, 158, 401
CsiI ACCWGGT 1 cut(s) 403
Csp6I GTAC 1 cut(s) 301
CviJI RGCY 4 cut(s) 80, 91, 127, 198
CviKI_1 RGCY 4 cut(s) 80, 91, 127, 198
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 1 cut(s) 363
DpnI GATC 2 cut(s) 34, 69
DpnII GATC 2 cut(s) 32, 67
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 7, 201
Eco32I GATATC 1 cut(s) 413
Eco47I GGWCC 3 cut(s) 11, 158, 401
EcoO109I RGGNCCY 3 cut(s) 11, 158, 401
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 1 cut(s) 413
EcoT14I CCWWGG 2 cut(s) 7, 201
ErhI CCWWGG 2 cut(s) 7, 201
FaiI YATR 1 cut(s) 195
FaqI GGGAC 3 cut(s) 95, 244, 411
FspBI CTAG 2 cut(s) 8, 420
HaeIII GGCC 1 cut(s) 127
HapII CCGG 1 cut(s) 113
HinfI GANTC 2 cut(s) 214, 273
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 221, 287
Hpy188I TCNGA 1 cut(s) 150
Hpy8I GTNNAC 2 cut(s) 221, 287
HpyAV CCTTC 1 cut(s) 202
HpyCH4IV ACGT 3 cut(s) 94, 108, 258
HpyCH4V TGCA 3 cut(s) 164, 292, 350
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 3 cut(s) 94, 108, 258
Kzo9I GATC 2 cut(s) 32, 67
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 8 cut(s) 27, 126, 174, 213, 219, 318, 390, 417
LweI GCATC 1 cut(s) 66
MabI ACCWGGT 1 cut(s) 403
MaeI CTAG 2 cut(s) 8, 420
MaeII ACGT 3 cut(s) 94, 108, 258
MaeIII GTNAC 1 cut(s) 254
MalI GATC 2 cut(s) 34, 69
MboI GATC 2 cut(s) 32, 67
MboII GAAGA 1 cut(s) 130
MfeI CAATTG 1 cut(s) 82
MflI RGATCY 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 1 cut(s) 82
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 1 cut(s) 208
MnlI CCTC 5 cut(s) 20, 23, 181, 392, 426
Mox20I TGGCCA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 127
Msp20I TGGCCA 1 cut(s) 127
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 2 cut(s) 113, 405
MunI CAATTG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 405
NciI CCSGG 1 cut(s) 113
NdeII GATC 2 cut(s) 32, 67
NlaIV GGNNCC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 273
PflFI GACNNNGTC 1 cut(s) 405
PflMI CCANNNNNTGG 2 cut(s) 87, 227
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Ppu21I YACGTR 1 cut(s) 259
PpuMI RGGWCCY 3 cut(s) 11, 158, 401
Psp5II RGGWCCY 3 cut(s) 11, 158, 401
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 3 cut(s) 11, 158, 401
PspPPI RGGWCCY 3 cut(s) 11, 158, 401
PsuI RGATCY 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 405
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
Sau3AI GATC 2 cut(s) 32, 67
Sau96I GGNCC 3 cut(s) 11, 158, 401
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 2 cut(s) 113, 405
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 1 cut(s) 66
SinI GGWCC 3 cut(s) 11, 158, 401
Sse9I AATT 1 cut(s) 82
SspMI CTAG 2 cut(s) 8, 420
StyD4I CCNGG 2 cut(s) 111, 403
StyI CCWWGG 2 cut(s) 7, 201
TaiI ACGT 3 cut(s) 97, 111, 261
TaqI TCGA 2 cut(s) 327, 409
TasI AATT 1 cut(s) 82
TfiI GAWTC 1 cut(s) 273
TscAI CASTG 3 cut(s) 232, 294, 357
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 3 cut(s) 232, 294, 357
Tth111I GACNNNGTC 1 cut(s) 405
Van91I CCANNNNNTGG 2 cut(s) 87, 227
VpaK11BI GGWCC 3 cut(s) 11, 158, 401
XmaJI CCTAGG 1 cut(s) 7
XspI CTAG 2 cut(s) 8, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.