Rh6CG508800

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0017074
Physical Location & Seq
Reverse (-)
66946115 .. 66946933
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG508800.1

Sequence Viewer

Length: 603 bp
ATGAGTACACTGCTGCTCAAATCTGGCTTAAGAATGGACCTGGAAATGCCTTTAAACCCAAAGTTGTATGGTGATTCAGCTACTCGACTTTTCGTGTATTGGACAGTAGTCTCGTACAAATCAACAGGTTGCTTTGATCTAACTTGCTCAGGATTTGTGCAAACTAATAAAGACATAGCCCTTGGTATGCCCCTAGGACCCGTATCAAGTGAAGGAGGACCCCAATATCAAACCACTTTCAGCATCAACAAGGATCAAAACACAGGCAATTGGTGGCTACGTTTGGGTCAAAACGTCCCGGTAGGATATTGGCCACCAAGTCTCTTCTCCTATCTGAAACAAGGTCCTGCAACATTGGTTGAATGGGGTGGGGAGGTGTATAGCTCCAAGGTGAAGGAGACTCGTCCACACACTGGACACACTGAGACTGGCAAAGATGGAAGTGCTTGTTACGTGAAACAAGTGAGAATCGTGGATTGTTCACTGCAACTAAAGTACCCAGAATGGGTAGGCACTTTTGTCGTTGAAGCGTATTGCTACTCCGCACTGAACTACGCTCAGTCGCTCGCCCAAGTTTGGAGGACCTGGTCGATATCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

200

Amino Acids

22.35

Weight (kDa)

8.21

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 18 - 188 5.2e-55 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 413
AciI CCGC 1 cut(s) 543
AclWI GGATC 1 cut(s) 261
AcoI YGGCCR 1 cut(s) 311
AfaI GTAC 3 cut(s) 7, 116, 497
AfiI CCNNNNNNNGG 3 cut(s) 413, 505, 576
AflII CTTAAG 1 cut(s) 28
AgsI TTSAA 2 cut(s) 362, 527
AjnI CCWGG 2 cut(s) 39, 584
AluBI AGCT 2 cut(s) 80, 384
AluI AGCT 2 cut(s) 80, 384
Alw26I GTCTC 4 cut(s) 115, 326, 392, 419
AlwI GGATC 1 cut(s) 261
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 193
AspS9I GGNCC 5 cut(s) 37, 197, 218, 344, 582
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 83, 403
AvaII GGWCC 5 cut(s) 37, 197, 218, 344, 582
AvrII CCTAGG 1 cut(s) 193
BaeI ACNNNNGTAYC 2 cut(s) 479, 512
BalI TGGCCA 1 cut(s) 313
BccI CCATC 1 cut(s) 431
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 2 cut(s) 41, 586
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 4 cut(s) 115, 326, 392, 419
BfaI CTAG 2 cut(s) 194, 601
BfrI CTTAAG 1 cut(s) 28
BisI GCNGC 1 cut(s) 14
BlnI CCTAGG 1 cut(s) 193
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 3 cut(s) 41, 299, 586
Bme18I GGWCC 5 cut(s) 37, 197, 218, 344, 582
BmgT120I GGNCC 5 cut(s) 37, 197, 218, 344, 582
BmiI GGNNCC 2 cut(s) 199, 220
BmrFI CCNGG 3 cut(s) 41, 299, 586
BmsI GCATC 1 cut(s) 252
BoxI GACNNNNGTC 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 454
BsaJI CCNNGG 3 cut(s) 181, 193, 387
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 505, 576
Bse1I ACTGG 2 cut(s) 418, 433
BseBI CCWGG 2 cut(s) 41, 586
BseDI CCNNGG 3 cut(s) 181, 193, 387
BseLI CCNNNNNNNGG 3 cut(s) 413, 505, 576
BseMII CTCAG 3 cut(s) 162, 414, 572
BseNI ACTGG 2 cut(s) 418, 433
BshFI GGCC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 3 cut(s) 413, 505, 576
BsmAI GTCTC 4 cut(s) 115, 326, 392, 419
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 136, 253
BspACI CCGC 1 cut(s) 543
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 3 cut(s) 161, 415, 571
BspLI GGNNCC 2 cut(s) 199, 220
BspPI GGATC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 28
BsrI ACTGG 2 cut(s) 418, 433
BssECI CCNNGG 3 cut(s) 181, 193, 387
BssMI GATC 2 cut(s) 136, 253
BssT1I CCWWGG 3 cut(s) 181, 193, 387
Bst2UI CCWGG 2 cut(s) 41, 586
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 329
BstAFI CTTAAG 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 3 cut(s) 148, 423, 558
BstKTI GATC 2 cut(s) 139, 256
BstMAI GTCTC 4 cut(s) 115, 326, 392, 419
BstMBI GATC 2 cut(s) 136, 253
BstNI CCWGG 2 cut(s) 41, 586
BstPAI GACNNNNGTC 1 cut(s) 107
BstSCI CCNGG 3 cut(s) 39, 297, 584
BsuRI GGCC 1 cut(s) 313
BtsI GCAGTG 2 cut(s) 8, 482
BtsIMutI CAGTG 5 cut(s) 8, 411, 420, 482, 545
Cac8I GCNNGC 1 cut(s) 567
Cfr13I GGNCC 5 cut(s) 37, 197, 218, 344, 582
CsiI ACCWGGT 1 cut(s) 584
Csp6I GTAC 3 cut(s) 6, 115, 496
CviJI RGCY 6 cut(s) 27, 80, 179, 277, 313, 384
CviKI_1 RGCY 6 cut(s) 27, 80, 179, 277, 313, 384
CviQI GTAC 3 cut(s) 6, 115, 496
DdeI CTNAG 3 cut(s) 148, 423, 558
DpnI GATC 2 cut(s) 138, 255
DpnII GATC 2 cut(s) 136, 253
DraI TTTAAA 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 3 cut(s) 181, 193, 387
Eco32I GATATC 1 cut(s) 594
Eco47I GGWCC 5 cut(s) 37, 197, 218, 344, 582
EcoO109I RGGNCCY 4 cut(s) 197, 218, 344, 582
EcoRII CCWGG 2 cut(s) 39, 584
EcoRV GATATC 1 cut(s) 594
EcoT14I CCWWGG 3 cut(s) 181, 193, 387
ErhI CCWWGG 3 cut(s) 181, 193, 387
FaiI YATR 4 cut(s) 69, 176, 188, 381
FaqI GGGAC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 14
Fsp4HI GCNGC 1 cut(s) 14
FspBI CTAG 2 cut(s) 194, 601
GluI GCNGC 1 cut(s) 14
HaeIII GGCC 1 cut(s) 313
HapII CCGG 1 cut(s) 299
HinfI GANTC 3 cut(s) 74, 400, 468
HpaII CCGG 1 cut(s) 299
HphI GGTGA 2 cut(s) 83, 403
Hpy166II GTNNAC 3 cut(s) 8, 407, 482
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 3 cut(s) 8, 407, 482
HpyAV CCTTC 2 cut(s) 206, 388
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 3 cut(s) 280, 294, 453
HpyCH4V TGCA 3 cut(s) 160, 350, 487
HpyF3I CTNAG 3 cut(s) 148, 423, 558
HpySE526I ACGT 3 cut(s) 280, 294, 453
Kzo9I GATC 2 cut(s) 136, 253
LmnI GCTCC 1 cut(s) 389
LweI GCATC 1 cut(s) 252
MabI ACCWGGT 1 cut(s) 584
MaeI CTAG 2 cut(s) 194, 601
MaeII ACGT 3 cut(s) 280, 294, 453
MaeIII GTNAC 1 cut(s) 449
MalI GATC 2 cut(s) 138, 255
MboI GATC 2 cut(s) 136, 253
MboII GAAGA 1 cut(s) 316
MfeI CAATTG 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 313
MluCI AATT 1 cut(s) 268
MluNI TGGCCA 1 cut(s) 313
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 3 cut(s) 209, 367, 573
Mox20I TGGCCA 1 cut(s) 313
MscI TGGCCA 1 cut(s) 313
MseI TTAA 2 cut(s) 29, 53
Msp20I TGGCCA 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 28
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 3 cut(s) 41, 299, 586
MunI CAATTG 1 cut(s) 268
MvaI CCWGG 2 cut(s) 41, 586
NciI CCSGG 1 cut(s) 299
NdeII GATC 2 cut(s) 136, 253
NlaIV GGNNCC 2 cut(s) 199, 220
PfeI GAWTC 2 cut(s) 74, 468
PflFI GACNNNGTC 1 cut(s) 586
PflMI CCANNNNNTGG 1 cut(s) 413
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 454
PpuMI RGGWCCY 4 cut(s) 197, 218, 344, 582
PshAI GACNNNNGTC 1 cut(s) 107
Psp5II RGGWCCY 4 cut(s) 197, 218, 344, 582
Psp6I CCWGG 2 cut(s) 39, 584
PspGI CCWGG 2 cut(s) 39, 584
PspN4I GGNNCC 2 cut(s) 199, 220
PspPI GGNCC 5 cut(s) 37, 197, 218, 344, 582
PspPPI RGGWCCY 4 cut(s) 197, 218, 344, 582
PsyI GACNNNGTC 1 cut(s) 586
RsaI GTAC 3 cut(s) 7, 116, 497
RsaNI GTAC 3 cut(s) 6, 115, 496
SaqAI TTAA 2 cut(s) 29, 53
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 2 cut(s) 136, 253
Sau96I GGNCC 5 cut(s) 37, 197, 218, 344, 582
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 41, 299, 586
SexAI ACCWGGT 1 cut(s) 584
SfaNI GCATC 1 cut(s) 252
SinI GGWCC 5 cut(s) 37, 197, 218, 344, 582
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 1 cut(s) 268
SsiI CCGC 1 cut(s) 543
SspMI CTAG 2 cut(s) 194, 601
StyD4I CCNGG 3 cut(s) 39, 297, 584
StyI CCWWGG 3 cut(s) 181, 193, 387
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 3 cut(s) 283, 297, 456
TaqI TCGA 2 cut(s) 85, 590
TasI AATT 1 cut(s) 268
TatI WGTACW 1 cut(s) 5
TfiI GAWTC 2 cut(s) 74, 468
Tru1I TTAA 2 cut(s) 29, 53
Tru9I TTAA 2 cut(s) 29, 53
TscAI CASTG 5 cut(s) 15, 418, 427, 489, 552
TseI GCWGC 1 cut(s) 13
TspRI CASTG 5 cut(s) 15, 418, 427, 489, 552
Tth111I GACNNNGTC 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 413
Vha464I CTTAAG 1 cut(s) 28
VpaK11BI GGWCC 5 cut(s) 37, 197, 218, 344, 582
XmaJI CCTAGG 1 cut(s) 193
XspI CTAG 2 cut(s) 194, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.