Rh6BG503600

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017074
Physical Location & Seq
Reverse (-)
70254156 .. 70254941
786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG503600.1

Sequence Viewer

Length: 570 bp
ATGGACCTGGAAATGCCTTTAAACCCAAAGTTGTATGGTGATTCAGCTACTCGACTTTTCGTGTATTGGACAGTAGTCTCGTACAAATCAACAGGTTGCTTTGATCTAACTTGCTCAGGATTTGTGCAAACTAATAAAGACATAGCCCTTGGTATGCCCCTAGGACCCGTATCAAGTGAAGGAGGACCCCAATATCAAACCACTTTCAGCATCAACAAGGATCAAAACACAGGCAATTGGTGGCTACGTTTGGGTCAAAACGTCCCGGTAGGATATTGGCCACCAAGTCTCTTCTCCTATCTGAAACAAGGTCCTGCAACATTGGTTGAATGGGGTGGGGAGGTGTATAGCTCCAAGGTGAAGGAGACTCGTCCACACACTGGACACACTGAGACTGGCAAAGATGGAAGTGCTTGTTACGTGAAACAAGTGAGAATCGTGGATTGTTCACTGCAACTTAAGTACCCAGAATGGGTAGGCACTTTTGTCGTTGAAGCGTATTGCTACTCCGCACTGAACTACGCTCAGTCACTCGCCCAAGTTTGGAGGACCTGGTCGATATCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

189

Amino Acids

21.15

Weight (kDa)

6.71

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 8 - 177 5.2e-55 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 380
AciI CCGC 1 cut(s) 510
AclWI GGATC 1 cut(s) 228
AcoI YGGCCR 1 cut(s) 278
AfaI GTAC 2 cut(s) 83, 464
AfiI CCNNNNNNNGG 3 cut(s) 380, 472, 543
AflII CTTAAG 1 cut(s) 458
AgsI TTSAA 2 cut(s) 329, 494
AjnI CCWGG 2 cut(s) 6, 551
AluBI AGCT 2 cut(s) 47, 351
AluI AGCT 2 cut(s) 47, 351
Alw26I GTCTC 4 cut(s) 82, 293, 359, 386
AlwI GGATC 1 cut(s) 228
AoxI GGCC 1 cut(s) 278
AspA2I CCTAGG 1 cut(s) 160
AspS9I GGNCC 5 cut(s) 4, 164, 185, 311, 549
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 2 cut(s) 50, 370
AvaII GGWCC 5 cut(s) 4, 164, 185, 311, 549
AvrII CCTAGG 1 cut(s) 160
BaeI ACNNNNGTAYC 2 cut(s) 446, 479
BalI TGGCCA 1 cut(s) 280
BccI CCATC 1 cut(s) 398
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 2 cut(s) 8, 553
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 4 cut(s) 82, 293, 359, 386
BfaI CTAG 2 cut(s) 161, 568
BfrI CTTAAG 1 cut(s) 458
BlnI CCTAGG 1 cut(s) 160
Bme1390I CCNGG 3 cut(s) 8, 266, 553
Bme18I GGWCC 5 cut(s) 4, 164, 185, 311, 549
BmgT120I GGNCC 5 cut(s) 4, 164, 185, 311, 549
BmiI GGNNCC 2 cut(s) 166, 187
BmrFI CCNGG 3 cut(s) 8, 266, 553
BmsI GCATC 1 cut(s) 219
BoxI GACNNNNGTC 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 266
BsaAI YACGTR 1 cut(s) 421
BsaJI CCNNGG 3 cut(s) 148, 160, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 380, 472, 543
Bse1I ACTGG 2 cut(s) 385, 400
BseBI CCWGG 2 cut(s) 8, 553
BseDI CCNNGG 3 cut(s) 148, 160, 354
BseLI CCNNNNNNNGG 3 cut(s) 380, 472, 543
BseMII CTCAG 3 cut(s) 129, 381, 539
BseNI ACTGG 2 cut(s) 385, 400
BshFI GGCC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 380, 472, 543
BsmAI GTCTC 4 cut(s) 82, 293, 359, 386
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 103, 220
BspACI CCGC 1 cut(s) 510
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 3 cut(s) 128, 382, 538
BspLI GGNNCC 2 cut(s) 166, 187
BspPI GGATC 1 cut(s) 228
BspTI CTTAAG 1 cut(s) 458
BsrI ACTGG 2 cut(s) 385, 400
BssECI CCNNGG 3 cut(s) 148, 160, 354
BssMI GATC 2 cut(s) 103, 220
BssT1I CCWWGG 3 cut(s) 148, 160, 354
Bst2UI CCWGG 2 cut(s) 8, 553
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 1 cut(s) 296
BstAFI CTTAAG 1 cut(s) 458
BstBAI YACGTR 1 cut(s) 421
BstDEI CTNAG 3 cut(s) 115, 390, 525
BstKTI GATC 2 cut(s) 106, 223
BstMAI GTCTC 4 cut(s) 82, 293, 359, 386
BstMBI GATC 2 cut(s) 103, 220
BstNI CCWGG 2 cut(s) 8, 553
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 3 cut(s) 6, 264, 551
BsuRI GGCC 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 4 cut(s) 378, 387, 449, 512
Cfr13I GGNCC 5 cut(s) 4, 164, 185, 311, 549
CsiI ACCWGGT 1 cut(s) 551
Csp6I GTAC 2 cut(s) 82, 463
CviJI RGCY 5 cut(s) 47, 146, 244, 280, 351
CviKI_1 RGCY 5 cut(s) 47, 146, 244, 280, 351
CviQI GTAC 2 cut(s) 82, 463
DdeI CTNAG 3 cut(s) 115, 390, 525
DpnI GATC 2 cut(s) 105, 222
DpnII GATC 2 cut(s) 103, 220
DraI TTTAAA 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 3 cut(s) 148, 160, 354
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 5 cut(s) 4, 164, 185, 311, 549
EcoO109I RGGNCCY 4 cut(s) 164, 185, 311, 549
EcoRII CCWGG 2 cut(s) 6, 551
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 3 cut(s) 148, 160, 354
ErhI CCWWGG 3 cut(s) 148, 160, 354
FaiI YATR 4 cut(s) 36, 143, 155, 348
FaqI GGGAC 1 cut(s) 248
FspBI CTAG 2 cut(s) 161, 568
HaeIII GGCC 1 cut(s) 280
HapII CCGG 1 cut(s) 266
HinfI GANTC 3 cut(s) 41, 367, 435
HpaII CCGG 1 cut(s) 266
HphI GGTGA 2 cut(s) 50, 370
Hpy166II GTNNAC 2 cut(s) 374, 449
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 1 cut(s) 117
Hpy8I GTNNAC 2 cut(s) 374, 449
HpyAV CCTTC 2 cut(s) 173, 355
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 3 cut(s) 247, 261, 420
HpyCH4V TGCA 3 cut(s) 127, 317, 454
HpyF3I CTNAG 3 cut(s) 115, 390, 525
HpySE526I ACGT 3 cut(s) 247, 261, 420
Kzo9I GATC 2 cut(s) 103, 220
LmnI GCTCC 1 cut(s) 356
LweI GCATC 1 cut(s) 219
MabI ACCWGGT 1 cut(s) 551
MaeI CTAG 2 cut(s) 161, 568
MaeII ACGT 3 cut(s) 247, 261, 420
MaeIII GTNAC 2 cut(s) 416, 528
MalI GATC 2 cut(s) 105, 222
MboI GATC 2 cut(s) 103, 220
MboII GAAGA 1 cut(s) 283
MfeI CAATTG 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 280
MluCI AATT 1 cut(s) 235
MluNI TGGCCA 1 cut(s) 280
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 3 cut(s) 176, 334, 540
Mox20I TGGCCA 1 cut(s) 280
MscI TGGCCA 1 cut(s) 280
MseI TTAA 2 cut(s) 20, 459
Msp20I TGGCCA 1 cut(s) 280
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 8, 266, 553
MunI CAATTG 1 cut(s) 235
MvaI CCWGG 2 cut(s) 8, 553
NciI CCSGG 1 cut(s) 266
NdeII GATC 2 cut(s) 103, 220
NlaIV GGNNCC 2 cut(s) 166, 187
NmuCI GTSAC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 41, 435
PflFI GACNNNGTC 1 cut(s) 553
PflMI CCANNNNNTGG 1 cut(s) 380
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 421
PpuMI RGGWCCY 4 cut(s) 164, 185, 311, 549
PshAI GACNNNNGTC 1 cut(s) 74
Psp5II RGGWCCY 4 cut(s) 164, 185, 311, 549
Psp6I CCWGG 2 cut(s) 6, 551
PspGI CCWGG 2 cut(s) 6, 551
PspN4I GGNNCC 2 cut(s) 166, 187
PspPI GGNCC 5 cut(s) 4, 164, 185, 311, 549
PspPPI RGGWCCY 4 cut(s) 164, 185, 311, 549
PsyI GACNNNGTC 1 cut(s) 553
RsaI GTAC 2 cut(s) 83, 464
RsaNI GTAC 2 cut(s) 82, 463
SaqAI TTAA 2 cut(s) 20, 459
Sau3AI GATC 2 cut(s) 103, 220
Sau96I GGNCC 5 cut(s) 4, 164, 185, 311, 549
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 3 cut(s) 8, 266, 553
SexAI ACCWGGT 1 cut(s) 551
SfaNI GCATC 1 cut(s) 219
SinI GGWCC 5 cut(s) 4, 164, 185, 311, 549
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 1 cut(s) 235
SsiI CCGC 1 cut(s) 510
SspMI CTAG 2 cut(s) 161, 568
StyD4I CCNGG 3 cut(s) 6, 264, 551
StyI CCWWGG 3 cut(s) 148, 160, 354
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 3 cut(s) 250, 264, 423
TaqI TCGA 2 cut(s) 52, 557
TasI AATT 1 cut(s) 235
TfiI GAWTC 2 cut(s) 41, 435
Tru1I TTAA 2 cut(s) 20, 459
Tru9I TTAA 2 cut(s) 20, 459
TscAI CASTG 4 cut(s) 385, 394, 456, 519
TseFI GTSAC 1 cut(s) 528
Tsp45I GTSAC 1 cut(s) 528
TspRI CASTG 4 cut(s) 385, 394, 456, 519
Tth111I GACNNNGTC 1 cut(s) 553
Van91I CCANNNNNTGG 1 cut(s) 380
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 5 cut(s) 4, 164, 185, 311, 549
XmaJI CCTAGG 1 cut(s) 160
XspI CTAG 2 cut(s) 161, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.