MD08G1074000.v1.1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017074
Physical Location & Seq
Forward (+)
5970178 .. 5972427
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1074000.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGAATTGCTCATTTGACACGTGGACTAAAACTGAAGCCATGAGGGCATGCACGCACGAAGCGGAGAGATCGATGATGGGGAAAAGGAGGAGTATATTGCTCTTGTTAGTAATGGGATTGTTTGTTTTTAGTGTTGGAGCAGCAGATCATCAGCCTCATGTGAATTCTAAAGCAAAGCAAGCTACTTTGGAAGTTCATAAGAAGTTGAAGCTTATCAATAAGCCCGGCGTGAAGACTATTAAGAGTGAGGATGGAGATATCGTTGATTGTGTTCGCATTTACAAACAGCCTGCTTTTGATCATCCAGCGTTGCGGAACCACAAAATTCAGATAACGCCGAATTTTCGAGAGGCTTCTGCTCCAAGCCCTAGTTCTAGTCCCAGTCCCAGTCCCAGTGCAAGTAATCATGTACAGAATGATCATTATTCTTCTCAGCAAGTATTATCTCAGATTTGGCAGAGGAGTGGAAGTTGTCCAGATGGAACCATTCCGATTAGGAGAATTCGAAGGCGAGACTTGCTGAGAGCTACTGGACTTGAACACTTTGGGAAGAAACCAGCAAATTCCTACCGCTCAACCAACGATGAGAAAGGCAGTGTAATCATCAATGGCACCCGAGTCGAACTTGGTCCTCAAGTCAATCGCTCAGCTGCTATCCTTGTAACAGTTGGATACAGTTATATAGGTGCTCAAGGAGATATAAATATTTGGAACCCAAAAGTTGAATCGCCAGATGAGTTCACTACTGCTCAAATATGGCTCAAGAATGGACCTGGTGACGCCTTTGAAAGCGTCGAATCAGGCTGGGTGGTAAATCCAAAGGTATACGGTGATGGATCAACTCGACTTTTCGTATATTGGACTAAGGACGCATACAAATCAACAGGCTGCTTTGATCTTACTTGCTCAGGATTTGTGCAAACGAACAAAGACATAGCCCTTGGCATGGCCCTTCAACCTATTTCATCTGCTTCGGGTCCACAATATCAGACCACCTTCAGCATCACCAAGGACTTCCACACGAACAACTGGTGGGTGCGCGTCGGCCAAAACATCCCCGTCGGATACTTTCCCGGAGAGCTCTTCTCTTACCTGACAAAGGGTGCTGCATCTTTAGTTGAGTGGGGTGGGGAGGTGTACAGCTCCAAGATTAAGAAAAACCATCCACACACTGCAACTGGAATGGGAAGCGGTGATTTTGCATGGAACAAAGAGGGGAATGCATGTTATATTAAGCAAGTTAGGATCATAGATTACTCACAGCAGCTCAAGTATCCTGAGTGGGTGGGGACTTTTACGGATGAAGAGTATTGCTACGCTGCAGTGAACTATGCAGCTTCACTTGCTGAAGAACCTGTGTTTTACTTCGCCGGCCCGGGTTTACATCCTCCTGATTGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

468

Amino Acids

51.8

Weight (kDa)

8.56

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 79 - 193 6.4e-34 Neprosin activation peptide
Neprosin PF03080 245 - 459 6.4e-69 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 611
AccBSI CCGCTC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 825
AccII CGCG 1 cut(s) 1041
AciI CCGC 4 cut(s) 62, 313, 571, 1191
AclWI GGATC 2 cut(s) 844, 1253
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 5 cut(s) 163, 324, 340, 501, 562
AcuI CTGAAG 3 cut(s) 54, 982, 1368
AcvI CACGTG 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 2 cut(s) 411, 1139
AfiI CCNNNNNNNGG 2 cut(s) 946, 1099
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 5 cut(s) 208, 539, 725, 788, 956
AjnI CCWGG 1 cut(s) 772
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AluBI AGCT 8 cut(s) 182, 211, 527, 650, 1081, 1143, 1267, 1337
AluI AGCT 8 cut(s) 182, 211, 527, 650, 1081, 1143, 1267, 1337
Alw21I GWGCWC 2 cut(s) 691, 1083
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 2 cut(s) 844, 1253
Ama87I CYCGRG 2 cut(s) 615, 1375
AoxI GGCC 3 cut(s) 948, 1045, 1372
ApeKI GCWGC 7 cut(s) 140, 650, 888, 1106, 1264, 1319, 1334
ApoI RAATTY 5 cut(s) 163, 324, 340, 501, 562
AspLEI GCGC 1 cut(s) 1041
AspS9I GGNCC 5 cut(s) 629, 770, 949, 977, 1373
AsuC2I CCSGG 4 cut(s) 225, 1074, 1376, 1377
AsuHPI GGTGA 4 cut(s) 788, 842, 997, 1205
AsuII TTCGAA 1 cut(s) 505
AvaI CYCGRG 2 cut(s) 615, 1375
AvaII GGWCC 3 cut(s) 629, 770, 977
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 1083
BbrPI CACGTG 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 691, 1083
BbvI GCAGC 7 cut(s) 152, 637, 875, 1093, 1276, 1306, 1346
BccI CCATC 5 cut(s) 70, 245, 473, 827, 1170
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 1 cut(s) 774
BciVI GTATCC 3 cut(s) 665, 1058, 1284
BclI TGATCA 2 cut(s) 298, 418
BcnI CCSGG 4 cut(s) 225, 1074, 1376, 1377
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 2 cut(s) 369, 375
BfmI CTRYAG 1 cut(s) 1320
BfuI GTATCC 3 cut(s) 665, 1058, 1284
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 7 cut(s) 141, 651, 889, 1107, 1265, 1320, 1335
BlpI GCTNAGC 1 cut(s) 646
BlsI GCNGC 7 cut(s) 142, 652, 890, 1108, 1266, 1321, 1336
Bme1390I CCNGG 5 cut(s) 225, 774, 1074, 1376, 1377
Bme18I GGWCC 3 cut(s) 629, 770, 977
BmeT110I CYCGRG 2 cut(s) 615, 1375
BmgT120I GGNCC 5 cut(s) 629, 770, 949, 977, 1373
BmiI GGNNCC 5 cut(s) 317, 484, 613, 713, 978
BmrFI CCNGG 5 cut(s) 225, 774, 1074, 1376, 1377
BmrI ACTGGG 3 cut(s) 375, 381, 387
BmsI GCATC 2 cut(s) 1011, 1118
BmuI ACTGGG 3 cut(s) 375, 381, 387
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 1070, 1102
Bpu10I CCTNAGC 1 cut(s) 907
Bpu1102I GCTNAGC 1 cut(s) 646
Bpu14I TTCGAA 1 cut(s) 505
BpuEI CTTGAG 4 cut(s) 618, 675, 746, 1253
BpuMI CCSGG 4 cut(s) 225, 1074, 1376, 1377
Bsa29I ATCGAT 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 940, 1008, 1375
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 946, 1099
Bse118I RCCGGY 1 cut(s) 1370
Bse1I ACTGG 6 cut(s) 381, 387, 393, 535, 1034, 1183
BseBI CCWGG 1 cut(s) 774
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 940, 1008, 1375
BseGI GGATG 6 cut(s) 256, 301, 1053, 1162, 1306, 1384
BseLI CCNNNNNNNGG 2 cut(s) 946, 1099
BseMII CTCAG 6 cut(s) 446, 461, 512, 660, 921, 1269
BseNI ACTGG 6 cut(s) 381, 387, 393, 535, 1034, 1183
BseRI GAGGAG 2 cut(s) 103, 475
BseXI GCAGC 7 cut(s) 152, 637, 875, 1093, 1276, 1306, 1346
BseYI CCCAGC 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 1041
BshFI GGCC 3 cut(s) 950, 1047, 1374
BshNI GGYRCC 1 cut(s) 611
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 691, 1083
BsiHKCI CYCGRG 2 cut(s) 615, 1375
BsiSI CCGG 4 cut(s) 225, 1074, 1371, 1376
BslFI GGGAC 4 cut(s) 363, 369, 375, 1303
BslI CCNNNNNNNGG 2 cut(s) 946, 1099
BsmAI GTCTC 1 cut(s) 507
BsmFI GGGAC 4 cut(s) 363, 369, 375, 1303
BsmI GAATGC 1 cut(s) 1225
BsnI GGCC 3 cut(s) 950, 1047, 1374
BsoBI CYCGRG 2 cut(s) 615, 1375
Bsp119I TTCGAA 1 cut(s) 505
Bsp1286I GDGCHC 2 cut(s) 691, 1083
Bsp1407I TGTACA 2 cut(s) 409, 1137
Bsp143I GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
Bsp1720I GCTNAGC 1 cut(s) 646
BspACI CCGC 4 cut(s) 62, 313, 571, 1191
BspANI GGCC 3 cut(s) 950, 1047, 1374
BspCNI CTCAG 6 cut(s) 445, 460, 513, 659, 920, 1270
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 1 cut(s) 1041
BspLI GGNNCC 5 cut(s) 317, 484, 613, 713, 978
BspMAI CTGCAG 1 cut(s) 1324
BspPI GGATC 2 cut(s) 844, 1253
BspQI GCTCTTC 1 cut(s) 1088
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 611
BsrBI CCGCTC 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 1370
BsrGI TGTACA 2 cut(s) 409, 1137
BsrI ACTGG 6 cut(s) 381, 387, 393, 535, 1034, 1183
BssAI RCCGGY 1 cut(s) 1370
BssECI CCNNGG 3 cut(s) 940, 1008, 1375
BssMI GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
BssNAI GTATAC 1 cut(s) 826
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 940, 1008
Bst1107I GTATAC 1 cut(s) 826
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 3 cut(s) 667, 677, 830
Bst6I CTCTTC 2 cut(s) 1088, 1299
BstACI GRCGYC 1 cut(s) 780
BstAUI TGTACA 2 cut(s) 409, 1137
BstBAI YACGTR 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 5 cut(s) 49, 53, 180, 291, 1372
BstDEI CTNAG 7 cut(s) 432, 447, 521, 646, 864, 907, 1278
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 6 cut(s) 256, 301, 1053, 1162, 1306, 1384
BstFNI CGCG 1 cut(s) 1041
BstHHI GCGC 1 cut(s) 1041
BstKTI GATC 7 cut(s) 71, 148, 301, 421, 839, 898, 1248
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
BstMWI GCNNNNNNNGC 4 cut(s) 44, 179, 517, 1343
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 2 cut(s) 51, 1227
BstSCI CCNGG 5 cut(s) 223, 772, 1072, 1374, 1375
BstSFI CTRYAG 1 cut(s) 1320
BstUI CGCG 1 cut(s) 1041
BstV1I GCAGC 7 cut(s) 152, 637, 875, 1093, 1276, 1306, 1346
BstV2I GAAGAC 1 cut(s) 239
BstZ17I GTATAC 1 cut(s) 826
Bsu15I ATCGAT 1 cut(s) 71
BsuI GTATCC 3 cut(s) 665, 1058, 1284
BsuRI GGCC 3 cut(s) 950, 1047, 1374
BsuTUI ATCGAT 1 cut(s) 71
BtsCI GGATG 6 cut(s) 256, 301, 1053, 1162, 1306, 1384
BtsI GCAGTG 3 cut(s) 601, 1170, 1329
BtsIMutI CAGTG 4 cut(s) 400, 601, 1170, 1329
Cac8I GCNNGC 5 cut(s) 49, 53, 180, 291, 1372
CfoI GCGC 1 cut(s) 1041
Cfr10I RCCGGY 1 cut(s) 1370
Cfr13I GGNCC 5 cut(s) 629, 770, 949, 977, 1373
Cfr9I CCCGGG 1 cut(s) 1375
ClaI ATCGAT 1 cut(s) 71
CseI GACGC 4 cut(s) 781, 788, 878, 1030
CsiI ACCWGGT 1 cut(s) 772
Csp6I GTAC 2 cut(s) 410, 1138
CviAII CATG 7 cut(s) 40, 48, 158, 407, 946, 1203, 1224
CviQI GTAC 2 cut(s) 410, 1138
DdeI CTNAG 7 cut(s) 432, 447, 521, 646, 864, 907, 1278
DpnI GATC 7 cut(s) 70, 147, 300, 420, 838, 897, 1247
DpnII GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 2 cut(s) 1088, 1299
EarI CTCTTC 2 cut(s) 1088, 1299
Ecl136II GAGCTC 1 cut(s) 1081
Eco130I CCWWGG 2 cut(s) 940, 1008
Eco24I GRGCYC 1 cut(s) 1083
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 3 cut(s) 629, 770, 977
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 3 cut(s) 54, 982, 1368
Eco72I CACGTG 1 cut(s) 21
Eco88I CYCGRG 2 cut(s) 615, 1375
EcoICRI GAGCTC 1 cut(s) 1081
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoRI GAATTC 2 cut(s) 163, 501
EcoRII CCWGG 1 cut(s) 772
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 940, 1008
EcoT22I ATGCAT 1 cut(s) 1225
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 2 cut(s) 940, 1008
FaeI CATG 7 cut(s) 43, 51, 161, 410, 949, 1206, 1227
FaqI GGGAC 4 cut(s) 363, 369, 375, 1303
FatI CATG 7 cut(s) 39, 47, 157, 406, 945, 1202, 1223
FbaI TGATCA 2 cut(s) 298, 418
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 7 cut(s) 141, 651, 889, 1107, 1265, 1320, 1335
FokI GGATG 6 cut(s) 263, 288, 1040, 1149, 1313, 1371
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 7 cut(s) 141, 651, 889, 1107, 1265, 1320, 1335
FspBI CTAG 2 cut(s) 369, 375
GlaI GCGC 1 cut(s) 1040
GluI GCNGC 7 cut(s) 141, 651, 889, 1107, 1265, 1320, 1335
GsaI CCCAGC 1 cut(s) 808
HaeIII GGCC 3 cut(s) 950, 1047, 1374
HapII CCGG 4 cut(s) 225, 1074, 1371, 1376
HgaI GACGC 4 cut(s) 781, 788, 878, 1030
HhaI GCGC 1 cut(s) 1041
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 7 cut(s) 43, 51, 161, 410, 949, 1206, 1227
Hin6I GCGC 1 cut(s) 1039
HinP1I GCGC 1 cut(s) 1039
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 3 cut(s) 618, 725, 797
HpaII CCGG 4 cut(s) 225, 1074, 1371, 1376
HphI GGTGA 4 cut(s) 788, 842, 997, 1205
Hpy166II GTNNAC 7 cut(s) 24, 741, 826, 980, 1138, 1327, 1382
Hpy188I TCNGA 5 cut(s) 330, 450, 492, 990, 1064
Hpy188III TCNNGA 6 cut(s) 347, 476, 763, 909, 1277, 1391
Hpy8I GTNNAC 7 cut(s) 24, 741, 826, 980, 1138, 1327, 1382
Hpy99I CGWCG 3 cut(s) 797, 1046, 1064
HpyAV CCTTC 3 cut(s) 501, 962, 1006
HpyCH4III ACNGT 3 cut(s) 667, 677, 830
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 9 cut(s) 51, 398, 919, 1109, 1175, 1202, 1223, 1322, 1334
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 179, 517, 1343
HpyF3I CTNAG 7 cut(s) 432, 447, 521, 646, 864, 907, 1278
HpySE526I ACGT 1 cut(s) 20
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 7 cut(s) 43, 51, 161, 410, 949, 1206, 1227
HspAI GCGC 1 cut(s) 1039
KroI GCCGGC 1 cut(s) 1370
KroNI GCCGGC 1 cut(s) 1372
Ksp22I TGATCA 2 cut(s) 298, 418
Kzo9I GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
LguI GCTCTTC 1 cut(s) 1088
LmnI GCTCC 3 cut(s) 137, 364, 1148
Lsp1109I GCAGC 7 cut(s) 152, 637, 875, 1093, 1276, 1306, 1346
LweI GCATC 2 cut(s) 1011, 1118
MabI ACCWGGT 1 cut(s) 772
MaeI CTAG 2 cut(s) 369, 375
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 3 cut(s) 661, 776, 1397
MalI GATC 7 cut(s) 70, 147, 300, 420, 838, 897, 1247
MbiI CCGCTC 1 cut(s) 573
MboI GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
MboII GAAGA 6 cut(s) 244, 420, 562, 1075, 1316, 1361
MhlI GDGCHC 2 cut(s) 691, 1083
MluCI AATT 6 cut(s) 4, 163, 324, 340, 501, 562
MlyI GAGTC 1 cut(s) 627
MmeI TCCRAC 3 cut(s) 115, 649, 1042
Mph1103I ATGCAT 1 cut(s) 1225
MroNI GCCGGC 1 cut(s) 1370
MseI TTAA 3 cut(s) 240, 1152, 1233
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 4 cut(s) 225, 1074, 1371, 1376
MspR9I CCNGG 5 cut(s) 225, 774, 1074, 1376, 1377
Mva1269I GAATGC 1 cut(s) 1225
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 1 cut(s) 1041
MwoI GCNNNNNNNGC 4 cut(s) 44, 179, 517, 1343
NaeI GCCGGC 1 cut(s) 1372
NciI CCSGG 4 cut(s) 225, 1074, 1376, 1377
NdeII GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
NgoMIV GCCGGC 1 cut(s) 1370
NlaIII CATG 7 cut(s) 43, 51, 161, 410, 949, 1206, 1227
NlaIV GGNNCC 5 cut(s) 317, 484, 613, 713, 978
NmuCI GTSAC 1 cut(s) 776
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 2 cut(s) 51, 1227
NspV TTCGAA 1 cut(s) 505
PaeI GCATGC 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 1088
PctI GAATGC 1 cut(s) 1225
PdiI GCCGGC 1 cut(s) 1372
PfeI GAWTC 2 cut(s) 725, 797
PfoI TCCNGGA 1 cut(s) 1072
PkrI GCNGC 7 cut(s) 142, 652, 890, 1108, 1266, 1321, 1336
PleI GAGTC 1 cut(s) 626
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
PpsI GAGTC 1 cut(s) 626
Ppu21I YACGTR 1 cut(s) 21
Psp124BI GAGCTC 1 cut(s) 1083
Psp6I CCWGG 1 cut(s) 772
PspCI CACGTG 1 cut(s) 21
PspFI CCCAGC 1 cut(s) 804
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 5 cut(s) 317, 484, 613, 713, 978
PspPI GGNCC 5 cut(s) 629, 770, 949, 977, 1373
PstI CTGCAG 1 cut(s) 1324
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 2 cut(s) 411, 1139
RsaNI GTAC 2 cut(s) 410, 1138
SacI GAGCTC 1 cut(s) 1083
SapI GCTCTTC 1 cut(s) 1088
SaqAI TTAA 3 cut(s) 240, 1152, 1233
SatI GCNGC 7 cut(s) 141, 651, 889, 1107, 1265, 1320, 1335
Sau3AI GATC 7 cut(s) 68, 145, 298, 418, 836, 895, 1245
Sau96I GGNCC 5 cut(s) 629, 770, 949, 977, 1373
SchI GAGTC 1 cut(s) 627
ScrFI CCNGG 5 cut(s) 225, 774, 1074, 1376, 1377
SduI GDGCHC 2 cut(s) 691, 1083
SexAI ACCWGGT 1 cut(s) 772
SfaNI GCATC 2 cut(s) 1011, 1118
SfcI CTRYAG 1 cut(s) 1320
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 3 cut(s) 629, 770, 977
SmaI CCCGGG 1 cut(s) 1377
SmlI CTYRAG 4 cut(s) 633, 690, 761, 1268
SmoI CTYRAG 4 cut(s) 633, 690, 761, 1268
SphI GCATGC 1 cut(s) 51
Sse9I AATT 6 cut(s) 4, 163, 324, 340, 501, 562
SsiI CCGC 4 cut(s) 62, 313, 571, 1191
SspI AATATT 1 cut(s) 706
SspMI CTAG 2 cut(s) 369, 375
SstI GAGCTC 1 cut(s) 1083
StyD4I CCNGG 5 cut(s) 223, 772, 1072, 1374, 1375
StyI CCWWGG 2 cut(s) 940, 1008
TaaI ACNGT 3 cut(s) 667, 677, 830
TaiI ACGT 1 cut(s) 23
TaqI TCGA 6 cut(s) 71, 346, 505, 621, 795, 844
TasI AATT 6 cut(s) 4, 163, 324, 340, 501, 562
TatI WGTACW 2 cut(s) 409, 1137
TfiI GAWTC 2 cut(s) 725, 797
Tru1I TTAA 3 cut(s) 240, 1152, 1233
Tru9I TTAA 3 cut(s) 240, 1152, 1233
TscAI CASTG 4 cut(s) 400, 601, 1177, 1329
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 7 cut(s) 140, 650, 888, 1106, 1264, 1319, 1334
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 4 cut(s) 17, 185, 954, 1317
TspGWI ACGGA 1 cut(s) 1313
TspMI CCCGGG 1 cut(s) 1375
TspRI CASTG 4 cut(s) 400, 601, 1177, 1329
VpaK11BI GGWCC 3 cut(s) 629, 770, 977
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 5 cut(s) 163, 324, 340, 501, 562
XceI RCATGY 2 cut(s) 51, 1227
XmaI CCCGGG 1 cut(s) 1375
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 2 cut(s) 369, 375
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.