AT5G25960

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017074
Physical Location & Seq
Reverse (-)
9062911 .. 9065011
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25960.1

Sequence Viewer

Length: 1074 bp
ATGAGATTGTTCTTAATATTTGCGATTCTGTGTGGTTTTTACAATGAAGCCTATGGGAAAGGTTCTTTAGATATCGATATGAAACTGAAGTCTCTTAATAAGCCTTCCTTGAAGACCATCAAGAGCGAAGATGGAGATATAATAGATTGTATAGATATCTACAAACAACATGCCTTTGATCATCCCGCTTTAAGGAACCACAAGATTCAGATGAAACCGAGCGTGGACTTTGGTACAAAGAAAACTACTATTCCAAACAACGGTTCTTCGGAACAAATAACGTCTCAAATTTGGTCTAAATCCGGAAATTGTCCCAAAGGGACAATACCGGAGGCTGTCCTTATGGCCTTAGGGTATAATTTTATTGGAGCTCAATCCGATATAAATGTATGGAACCCTCCGAGGGTTCAGGCTAGCGACTACAGCTCTGCTCAGATATGGCTGCTTGGTGGCCTCTCAGATACTTTTGAAAGCATAGAAGCTGGTTGGGCGGTGAATCCAAGTGTTTTCGGAGACTCCCGCACACGTCTTTTCACATATTGGACTAAAGATGGATACTCTAAAACGGGGTGCGTCAACCTCTTATGCGCAGGTTTTGTGCAAACAACTACTAAGTTTGCATTGGGTGCAGCAATAGAACCCGTTTCGACTACCTCGCAAAAACAACATTTCATCACCGTGAGCATGTTCTTGGATACCAACAGCGGGAATTGGTGGTTGACATGTGCTAACAACGTGATAGGTTATTGGCCGGGGACACTCTTCGCCTACCTCAAACATAGCGCAACCGCTGTGCAGTGTGGTGGAGAAGTACATAGCCCAAACGTGGGGAAGAAGCCACATACAAGAACTTCAATGGGGAGTGGGCAATGGGCCTCTTACCTCTGGGCTCAAGCTTGTTACCACAGCAACATTAGAATCAAGGACTATTCCTTGCAGATCAAATACCCCAAATATCTGTCCGAGTACGCTGATGAGTACGAGTGTTATTCTACAAAGCTCCACCGGAAAACATACATGTCAGAGCCCTACTTTTACTTTGGAGGACCTGGCCAGAATTCTCGTTGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

357

Amino Acids

39.91

Weight (kDa)

8.65

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 39 - 110 1.6e-25 Neprosin activation peptide
Neprosin PF03080 139 - 350 4.9e-72 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 589
Acc36I ACCTGC 1 cut(s) 581
AccIII TCCGGA 1 cut(s) 302
AciI CCGC 5 cut(s) 186, 491, 520, 705, 789
AcoI YGGCCR 2 cut(s) 749, 1051
AcsI RAATTY 2 cut(s) 288, 1057
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 4 cut(s) 235, 813, 968, 980
AfiI CCNNNNNNNGG 6 cut(s) 192, 260, 403, 705, 826, 827
AflIII ACRYGT 3 cut(s) 524, 722, 1017
AgsI TTSAA 3 cut(s) 112, 470, 855
AjiI CACGTC 1 cut(s) 527
AjnI CCWGG 1 cut(s) 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AluBI AGCT 5 cut(s) 371, 426, 482, 896, 1000
AluI AGCT 5 cut(s) 371, 426, 482, 896, 1000
Alw21I GWGCWC 1 cut(s) 373
Alw26I GTCTC 3 cut(s) 96, 288, 507
Aor13HI TCCGGA 1 cut(s) 302
AoxI GGCC 5 cut(s) 345, 451, 749, 873, 1051
ApeKI GCWGC 2 cut(s) 442, 629
ApoI RAATTY 2 cut(s) 288, 1057
AspLEI GCGC 2 cut(s) 590, 785
AspS9I GGNCC 2 cut(s) 873, 1046
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 2 cut(s) 505, 667
AsuNHI GCTAGC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 1046
AxyI CCTNAGG 1 cut(s) 349
BalI TGGCCA 1 cut(s) 1053
BanII GRGCYC 3 cut(s) 373, 892, 1029
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 373
BbvI GCAGC 2 cut(s) 429, 641
BccI CCATC 3 cut(s) 125, 125, 545
BciT130I CCWGG 1 cut(s) 1050
BciVI GTATCC 2 cut(s) 548, 688
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 1 cut(s) 753
BcoDI GTCTC 3 cut(s) 96, 288, 507
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 421
BfuAI ACCTGC 1 cut(s) 581
BfuI GTATCC 2 cut(s) 548, 688
BisI GCNGC 2 cut(s) 443, 630
BlsI GCNGC 2 cut(s) 444, 631
Bme1390I CCNGG 2 cut(s) 753, 1050
Bme18I GGWCC 1 cut(s) 1046
BmgBI CACGTC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 873, 1046
BmiI GGNNCC 2 cut(s) 197, 395
BmrFI CCNGG 2 cut(s) 753, 1050
BmtI GCTAGC 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 876
BpuMI CCSGG 1 cut(s) 753
Bsa29I ATCGAT 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 401, 752
BsaWI WCCGGW 3 cut(s) 302, 328, 1005
Bsc4I CCNNNNNNNGG 6 cut(s) 192, 260, 403, 705, 826, 827
Bse21I CCTNAGG 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 875
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 1 cut(s) 1050
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 401, 752
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 6 cut(s) 192, 260, 403, 705, 826, 827
BseMI GCAATG 1 cut(s) 875
BseMII CTCAG 2 cut(s) 446, 471
BseXI GCAGC 2 cut(s) 429, 641
BsgI GTGCAG 2 cut(s) 648, 815
BshFI GGCC 5 cut(s) 347, 453, 751, 875, 1053
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 373
BsiSI CCGG 4 cut(s) 303, 329, 752, 1006
BslFI GGGAC 3 cut(s) 297, 334, 769
BslI CCNNNNNNNGG 6 cut(s) 192, 260, 403, 705, 826, 827
BsmAI GTCTC 3 cut(s) 96, 288, 507
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 297, 334, 769
BsnI GGCC 5 cut(s) 347, 453, 751, 875, 1053
Bsp1286I GDGCHC 3 cut(s) 373, 892, 1029
Bsp13I TCCGGA 1 cut(s) 302
Bsp143I GATC 2 cut(s) 178, 939
BspACI CCGC 5 cut(s) 186, 491, 520, 705, 789
BspANI GGCC 5 cut(s) 347, 453, 751, 875, 1053
BspCNI CTCAG 2 cut(s) 445, 470
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 302
BspLI GGNNCC 2 cut(s) 197, 395
BspMI ACCTGC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 417
BsrDI GCAATG 1 cut(s) 875
BssECI CCNNGG 2 cut(s) 401, 752
BssMI GATC 2 cut(s) 178, 939
Bst2UI CCWGG 1 cut(s) 1050
Bst4CI ACNGT 2 cut(s) 263, 679
Bst6I CTCTTC 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 4 cut(s) 349, 432, 457, 612
BstF5I GGATG 1 cut(s) 181
BstHHI GCGC 2 cut(s) 590, 785
BstKTI GATC 2 cut(s) 181, 942
BstMAI GTCTC 3 cut(s) 96, 288, 507
BstMBI GATC 2 cut(s) 178, 939
BstMWI GCNNNNNNNGC 3 cut(s) 423, 488, 626
BstNI CCWGG 1 cut(s) 1050
BstNSI RCATGY 4 cut(s) 173, 688, 726, 1021
BstSCI CCNGG 2 cut(s) 751, 1048
BstSFI CTRYAG 1 cut(s) 421
BstV1I GCAGC 2 cut(s) 429, 641
BstV2I GAAGAC 1 cut(s) 119
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 349
BsuI GTATCC 2 cut(s) 548, 688
BsuRI GGCC 5 cut(s) 347, 453, 751, 875, 1053
BsuTUI ATCGAT 1 cut(s) 75
BtrI CACGTC 1 cut(s) 527
BtsCI GGATG 1 cut(s) 181
BtsI GCAGTG 1 cut(s) 803
BtsIMutI CAGTG 1 cut(s) 803
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 2 cut(s) 590, 785
Cfr13I GGNCC 2 cut(s) 873, 1046
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 234, 812, 967, 979
CviAII CATG 4 cut(s) 170, 685, 723, 1018
CviQI GTAC 4 cut(s) 234, 812, 967, 979
DdeI CTNAG 4 cut(s) 349, 432, 457, 612
DpnI GATC 2 cut(s) 180, 941
DpnII GATC 2 cut(s) 178, 939
EaeI YGGCCR 2 cut(s) 749, 1051
Eam1104I CTCTTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 767
Ecl136II GAGCTC 1 cut(s) 371
Eco24I GRGCYC 3 cut(s) 373, 892, 1029
Eco32I GATATC 2 cut(s) 73, 157
Eco47I GGWCC 1 cut(s) 1046
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 107
Eco81I CCTNAGG 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 1046
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 1 cut(s) 1048
EcoRV GATATC 2 cut(s) 73, 157
EcoT38I GRGCYC 3 cut(s) 373, 892, 1029
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 4 cut(s) 173, 688, 726, 1021
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 3 cut(s) 297, 334, 769
FatI CATG 4 cut(s) 169, 684, 722, 1017
FauI CCCGC 3 cut(s) 193, 527, 698
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 443, 630
FokI GGATG 1 cut(s) 168
FriOI GRGCYC 3 cut(s) 373, 892, 1029
Fsp4HI GCNGC 2 cut(s) 443, 630
FspBI CTAG 1 cut(s) 414
FspI TGCGCA 1 cut(s) 589
GlaI GCGC 2 cut(s) 589, 784
GluI GCNGC 2 cut(s) 443, 630
HaeIII GGCC 5 cut(s) 347, 453, 751, 875, 1053
HapII CCGG 4 cut(s) 303, 329, 752, 1006
HgaI GACGC 1 cut(s) 562
HhaI GCGC 2 cut(s) 590, 785
Hin1II CATG 4 cut(s) 173, 688, 726, 1021
Hin6I GCGC 2 cut(s) 588, 783
HinP1I GCGC 2 cut(s) 588, 783
HincII GTYRAC 2 cut(s) 577, 720
HindII GTYRAC 2 cut(s) 577, 720
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 5 cut(s) 25, 205, 496, 515, 918
HpaII CCGG 4 cut(s) 303, 329, 752, 1006
HphI GGTGA 2 cut(s) 505, 667
Hpy166II GTNNAC 3 cut(s) 226, 577, 720
Hpy188I TCNGA 9 cut(s) 210, 271, 379, 402, 435, 460, 512, 964, 1024
Hpy188III TCNNGA 2 cut(s) 121, 303
Hpy8I GTNNAC 3 cut(s) 226, 577, 720
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 2 cut(s) 263, 679
HpyCH4IV ACGT 4 cut(s) 281, 526, 735, 825
HpyCH4V TGCA 5 cut(s) 601, 620, 629, 796, 937
HpyF10VI GCNNNNNNNGC 3 cut(s) 423, 488, 626
HpyF3I CTNAG 4 cut(s) 349, 432, 457, 612
HpySE526I ACGT 4 cut(s) 281, 526, 735, 825
Hsp92II CATG 4 cut(s) 173, 688, 726, 1021
HspAI GCGC 2 cut(s) 588, 783
Kpn2I TCCGGA 1 cut(s) 302
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 2 cut(s) 178, 939
LmnI GCTCC 2 cut(s) 368, 1005
Lsp1109I GCAGC 2 cut(s) 429, 641
MaeI CTAG 1 cut(s) 414
MaeII ACGT 4 cut(s) 281, 526, 735, 825
MaeIII GTNAC 1 cut(s) 899
MalI GATC 2 cut(s) 180, 941
MboI GATC 2 cut(s) 178, 939
MboII GAAGA 5 cut(s) 124, 140, 258, 754, 844
MhlI GDGCHC 3 cut(s) 373, 892, 1029
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 5 cut(s) 288, 307, 358, 709, 1057
MluNI TGGCCA 1 cut(s) 1053
MlyI GAGTC 1 cut(s) 509
Mox20I TGGCCA 1 cut(s) 1053
MroI TCCGGA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 1053
MseI TTAA 4 cut(s) 14, 96, 191, 1072
MslI CAYNNNNRTG 1 cut(s) 677
Msp20I TGGCCA 1 cut(s) 1053
MspA1I CMGCKG 2 cut(s) 705, 791
MspI CCGG 4 cut(s) 303, 329, 752, 1006
MspR9I CCNGG 2 cut(s) 753, 1050
MvaI CCWGG 1 cut(s) 1050
MwoI GCNNNNNNNGC 3 cut(s) 423, 488, 626
NciI CCSGG 1 cut(s) 753
NdeII GATC 2 cut(s) 178, 939
NheI GCTAGC 1 cut(s) 413
NlaIII CATG 4 cut(s) 173, 688, 726, 1021
NlaIV GGNNCC 2 cut(s) 197, 395
NsbI TGCGCA 1 cut(s) 589
NspI RCATGY 4 cut(s) 173, 688, 726, 1021
PciI ACATGT 2 cut(s) 722, 1017
PfeI GAWTC 4 cut(s) 25, 205, 496, 918
PkrI GCNGC 2 cut(s) 444, 631
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PpuMI RGGWCCY 1 cut(s) 1046
PscI ACATGT 2 cut(s) 722, 1017
Psp124BI GAGCTC 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 1046
Psp6I CCWGG 1 cut(s) 1048
PspGI CCWGG 1 cut(s) 1048
PspN4I GGNNCC 2 cut(s) 197, 395
PspPI GGNCC 2 cut(s) 873, 1046
PspPPI RGGWCCY 1 cut(s) 1046
RsaI GTAC 4 cut(s) 235, 813, 968, 980
RsaNI GTAC 4 cut(s) 234, 812, 967, 979
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 4 cut(s) 14, 96, 191, 1072
SatI GCNGC 2 cut(s) 443, 630
Sau3AI GATC 2 cut(s) 178, 939
Sau96I GGNCC 2 cut(s) 873, 1046
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 2 cut(s) 753, 1050
SduI GDGCHC 3 cut(s) 373, 892, 1029
SfcI CTRYAG 1 cut(s) 421
SinI GGWCC 1 cut(s) 1046
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 891
SmoI CTYRAG 1 cut(s) 891
Sse9I AATT 5 cut(s) 288, 307, 358, 709, 1057
SsiI CCGC 5 cut(s) 186, 491, 520, 705, 789
SspI AATATT 1 cut(s) 18
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 2 cut(s) 751, 1048
TaaI ACNGT 2 cut(s) 263, 679
TaiI ACGT 4 cut(s) 284, 529, 738, 828
TaqI TCGA 2 cut(s) 75, 647
TasI AATT 5 cut(s) 288, 307, 358, 709, 1057
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 4 cut(s) 25, 205, 496, 918
Tru1I TTAA 4 cut(s) 14, 96, 191, 1072
Tru9I TTAA 4 cut(s) 14, 96, 191, 1072
TscAI CASTG 1 cut(s) 803
TseI GCWGC 2 cut(s) 442, 629
TspDTI ATGAA 4 cut(s) 60, 95, 227, 661
TspRI CASTG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 1046
XapI RAATTY 2 cut(s) 288, 1057
XceI RCATGY 4 cut(s) 173, 688, 726, 1021
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.