RLG00000010485

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017074
Physical Location & Seq
Forward (+)
2021666 .. 2023523
1858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010485

Sequence Viewer

Length: 1164 bp
ATGGAAAGGAGGTCTATAAAGCTTTTGCTAGCAATTATGGGTTTGATTGTTTTTAGTGGTAGTGAAGCAATGGATGTAGATGTGAATGCTAAAGCTAAGAAACATTCTTTGGAAATTCATAAAAAGTTGAAGCTTCTCAATAGGCGAGCAGTGAAAAGTACTAAGAGTGAGGATGGAGACATTGTTGACTGTGTTGACATCTACAAACAGCCTGCTTTTGATCATCCAGCATTGCAAAACCACAAAATTCAGATGAGACCCAGTTTTCAATTTGCATCACAGGCTCCTAGTCCTAGTGCTAGCCTCAGTCCTAGTCCTATTCCCGGTCCTAGCAGGAGTTCTGTACAAAATGATCACAATTCATCTCAGCTAATCTCGCAGAATTGGCAAAGAAGTGGAAACTACTTGTTAAGAGCCACTTCACTTGAACGCTTTGGGAAGAAAAACCCAGCACCAATTTCCTACTCAACCAATGATGACAAAGGGACTGTGTACATTAACCAGACTAGAGTCGAACTTGGTTCTCAAATCAATCGTTCTGCTGCGATTCTTGTGACTACTGGATACAGTTACATAGGAGCTCAGGGAGATATAAATATTTGGAATCCAAAAGTCGAATCACCGGATGAGTACACTACTGCTCAAATCTGGCTTAAGAATGGACCTGGAAATGCCTTTAAACCCAAAGTTGTATGGTGGACTCGTTACAAATCAACAGGTTGCTTTGATCTAACTTGCTCAGGATTTGTGCAAACTAATAAAGACATAGCCCTTGATCAAAACACAGGCAATTGGTGGCTACGTTTGGGTCAAAACGTCCCGGTAGGATATTGGCCACCAACTCTCTTCTCCTATCTGAAACAAGGTCCTGCAACATTGGTTGAATGGGGTGGGGAGGTGTATAGCTCCAAGGTGAAGGAGACTCGTCCACACACTGGACACACTGGGACTAGCAAAGATGGAAGTGCTTGTTACGTGAAACAAGTGAGAATCGTGGATTGTTCACTGCAACTAAAGTACCCAGAATGGGTAGGCACTTTTGTCGATGAAGCGTATTGCTACTCTGCACTGAACTACGCTCAGTCGCTTGCCCAGAACCTACATTTTACTTTGGAGGACCTGGTCGATGTCCTCCTAGTTGTCCCTGAAAGATGGATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

388

Amino Acids

43.31

Weight (kDa)

8.93

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 54 - 153 5.9e-18 Neprosin activation peptide
Neprosin PF03080 231 - 260 6.3e-07 Neprosin
Neprosin PF03080 256 - 369 1.8e-24 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 935
AcoI YGGCCR 1 cut(s) 833
AcsI RAATTY 2 cut(s) 114, 246
AfaI GTAC 5 cut(s) 160, 345, 494, 632, 1019
AfiI CCNNNNNNNGG 3 cut(s) 323, 935, 1027
AflII CTTAAG 1 cut(s) 653
AgsI TTSAA 4 cut(s) 130, 269, 428, 884
AjnI CCWGG 2 cut(s) 664, 1119
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 6 cut(s) 22, 95, 133, 370, 581, 906
AluI AGCT 6 cut(s) 22, 95, 133, 370, 581, 906
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 3 cut(s) 171, 250, 914
AoxI GGCC 1 cut(s) 833
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 2 cut(s) 114, 246
AspS9I GGNCC 4 cut(s) 326, 662, 866, 1117
AsuC2I CCSGG 2 cut(s) 324, 821
AsuHPI GGTGA 2 cut(s) 612, 925
AsuNHI GCTAGC 2 cut(s) 28, 299
AvaII GGWCC 4 cut(s) 326, 662, 866, 1117
BaeI ACNNNNGTAYC 2 cut(s) 1001, 1034
BalI TGGCCA 1 cut(s) 835
BanII GRGCYC 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 1 cut(s) 529
BccI CCATC 3 cut(s) 167, 953, 1146
BcgI CGANNNNNNTGC 2 cut(s) 1024, 1058
BciT130I CCWGG 2 cut(s) 666, 1121
BciVI GTATCC 1 cut(s) 557
BclI TGATCA 3 cut(s) 220, 352, 775
BcnI CCSGG 2 cut(s) 324, 821
BcoDI GTCTC 3 cut(s) 171, 250, 914
BfaI CTAG 9 cut(s) 29, 288, 294, 300, 312, 330, 507, 951, 1136
BfrI CTTAAG 1 cut(s) 653
BfuI GTATCC 1 cut(s) 557
BisI GCNGC 1 cut(s) 543
BlsI GCNGC 1 cut(s) 544
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 4 cut(s) 324, 666, 821, 1121
Bme18I GGWCC 4 cut(s) 326, 662, 866, 1117
BmgT120I GGNCC 4 cut(s) 326, 662, 866, 1117
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 4 cut(s) 324, 666, 821, 1121
BmrI ACTGGG 2 cut(s) 255, 954
BmsI GCATC 2 cut(s) 284, 1146
BmtI GCTAGC 2 cut(s) 32, 303
BmuI ACTGGG 2 cut(s) 255, 954
BoxI GACNNNNGTC 1 cut(s) 509
Bpu10I CCTNAGC 2 cut(s) 582, 739
BpuMI CCSGG 2 cut(s) 324, 821
BsaAI YACGTR 1 cut(s) 976
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 909
BsaWI WCCGGW 1 cut(s) 622
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 935, 1027
Bse1I ACTGG 4 cut(s) 261, 565, 940, 949
Bse3DI GCAATG 2 cut(s) 75, 230
BseBI CCWGG 2 cut(s) 666, 1121
BseDI CCNNGG 1 cut(s) 909
BseGI GGATG 5 cut(s) 79, 178, 223, 631, 1161
BseLI CCNNNNNNNGG 3 cut(s) 323, 935, 1027
BseMI GCAATG 2 cut(s) 75, 230
BseMII CTCAG 5 cut(s) 319, 380, 596, 753, 1094
BseNI ACTGG 4 cut(s) 261, 565, 940, 949
BseXI GCAGC 1 cut(s) 529
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 1050
BshFI GGCC 1 cut(s) 835
BsiHKAI GWGCWC 1 cut(s) 583
BsiSI CCGG 3 cut(s) 324, 623, 821
BslFI GGGAC 4 cut(s) 499, 803, 961, 1127
BslI CCNNNNNNNGG 3 cut(s) 323, 935, 1027
BsmAI GTCTC 3 cut(s) 171, 250, 914
BsmFI GGGAC 4 cut(s) 499, 803, 961, 1127
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 1 cut(s) 835
Bso31I GGTCTC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 583
Bsp1407I TGTACA 2 cut(s) 343, 492
Bsp143I GATC 4 cut(s) 220, 352, 727, 775
BspANI GGCC 1 cut(s) 835
BspCNI CTCAG 5 cut(s) 318, 379, 595, 752, 1093
BspLI GGNNCC 1 cut(s) 285
BspOI GCTAGC 2 cut(s) 32, 303
BspTI CTTAAG 1 cut(s) 653
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 2 cut(s) 75, 230
BsrGI TGTACA 2 cut(s) 343, 492
BsrI ACTGG 4 cut(s) 261, 565, 940, 949
BssECI CCNNGG 1 cut(s) 909
BssMI GATC 4 cut(s) 220, 352, 727, 775
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 666, 1121
Bst4CI ACNGT 3 cut(s) 191, 490, 569
Bst6I CTCTTC 1 cut(s) 851
BstAFI CTTAAG 1 cut(s) 653
BstAUI TGTACA 2 cut(s) 343, 492
BstBAI YACGTR 1 cut(s) 976
BstC8I GCNNGC 5 cut(s) 30, 147, 213, 301, 1089
BstDEI CTNAG 7 cut(s) 96, 162, 305, 366, 582, 739, 1080
BstF5I GGATG 5 cut(s) 79, 178, 223, 631, 1161
BstKTI GATC 4 cut(s) 223, 355, 730, 778
BstMAI GTCTC 3 cut(s) 171, 250, 914
BstMBI GATC 4 cut(s) 220, 352, 727, 775
BstMWI GCNNNNNNNGC 3 cut(s) 281, 376, 385
BstNI CCWGG 2 cut(s) 666, 1121
BstPAI GACNNNNGTC 1 cut(s) 509
BstSCI CCNGG 4 cut(s) 322, 664, 819, 1119
BstV1I GCAGC 1 cut(s) 529
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 1 cut(s) 835
BtsCI GGATG 5 cut(s) 79, 178, 223, 631, 1161
BtsI GCAGTG 2 cut(s) 156, 1004
BtsIMutI CAGTG 5 cut(s) 156, 933, 942, 1004, 1067
Cac8I GCNNGC 5 cut(s) 30, 147, 213, 301, 1089
Cfr13I GGNCC 4 cut(s) 326, 662, 866, 1117
CsiI ACCWGGT 1 cut(s) 1119
Csp6I GTAC 5 cut(s) 159, 344, 493, 631, 1018
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviQI GTAC 5 cut(s) 159, 344, 493, 631, 1018
DdeI CTNAG 7 cut(s) 96, 162, 305, 366, 582, 739, 1080
DpnI GATC 4 cut(s) 222, 354, 729, 777
DpnII GATC 4 cut(s) 220, 352, 727, 775
DraI TTTAAA 1 cut(s) 679
EaeI YGGCCR 1 cut(s) 833
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
Ecl136II GAGCTC 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 583
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 4 cut(s) 326, 662, 866, 1117
Eco53kI GAGCTC 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 581
EcoO109I RGGNCCY 2 cut(s) 866, 1117
EcoRII CCWGG 2 cut(s) 664, 1119
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 909
FaiI YATR 8 cut(s) 17, 38, 120, 575, 593, 694, 767, 903
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FaqI GGGAC 4 cut(s) 499, 803, 961, 1127
FbaI TGATCA 3 cut(s) 220, 352, 775
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 4 cut(s) 86, 185, 210, 638
FriOI GRGCYC 1 cut(s) 583
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 9 cut(s) 29, 288, 294, 300, 312, 330, 507, 951, 1136
GluI GCNGC 1 cut(s) 543
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 1 cut(s) 835
HapII CCGG 3 cut(s) 324, 623, 821
HincII GTYRAC 2 cut(s) 187, 196
HindII GTYRAC 2 cut(s) 187, 196
HindIII AAGCTT 2 cut(s) 20, 131
HinfI GANTC 7 cut(s) 510, 547, 604, 617, 700, 922, 990
HpaII CCGG 3 cut(s) 324, 623, 821
HphI GGTGA 2 cut(s) 612, 925
Hpy166II GTNNAC 7 cut(s) 187, 196, 493, 633, 699, 929, 1004
Hpy188I TCNGA 3 cut(s) 252, 858, 1163
Hpy188III TCNNGA 1 cut(s) 741
Hpy8I GTNNAC 7 cut(s) 187, 196, 493, 633, 699, 929, 1004
HpyAV CCTTC 1 cut(s) 910
HpyCH4III ACNGT 3 cut(s) 191, 490, 569
HpyCH4IV ACGT 3 cut(s) 802, 816, 975
HpyCH4V TGCA 6 cut(s) 235, 275, 751, 872, 1009, 1067
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 376, 385
HpyF3I CTNAG 7 cut(s) 96, 162, 305, 366, 582, 739, 1080
HpySE526I ACGT 3 cut(s) 802, 816, 975
Ksp22I TGATCA 3 cut(s) 220, 352, 775
Kzo9I GATC 4 cut(s) 220, 352, 727, 775
LmnI GCTCC 3 cut(s) 289, 578, 911
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 2 cut(s) 284, 1146
MabI ACCWGGT 1 cut(s) 1119
MaeI CTAG 9 cut(s) 29, 288, 294, 300, 312, 330, 507, 951, 1136
MaeII ACGT 3 cut(s) 802, 816, 975
MaeIII GTNAC 4 cut(s) 553, 569, 704, 971
MalI GATC 4 cut(s) 222, 354, 729, 777
MboI GATC 4 cut(s) 220, 352, 727, 775
MboII GAAGA 2 cut(s) 451, 838
MfeI CAATTG 1 cut(s) 790
MhlI GDGCHC 1 cut(s) 583
MlsI TGGCCA 1 cut(s) 835
MluCI AATT 8 cut(s) 33, 114, 246, 269, 358, 382, 456, 790
MluNI TGGCCA 1 cut(s) 835
MlyI GAGTC 3 cut(s) 519, 694, 916
MnlI CCTC 6 cut(s) 3, 163, 314, 889, 1108, 1142
Mox20I TGGCCA 1 cut(s) 835
MscI TGGCCA 1 cut(s) 835
MseI TTAA 4 cut(s) 410, 498, 654, 678
Msp20I TGGCCA 1 cut(s) 835
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 3 cut(s) 324, 623, 821
MspR9I CCNGG 4 cut(s) 324, 666, 821, 1121
MunI CAATTG 1 cut(s) 790
Mva1269I GAATGC 1 cut(s) 91
MvaI CCWGG 2 cut(s) 666, 1121
MwoI GCNNNNNNNGC 3 cut(s) 281, 376, 385
NciI CCSGG 2 cut(s) 324, 821
NdeII GATC 4 cut(s) 220, 352, 727, 775
NheI GCTAGC 2 cut(s) 28, 299
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 553
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 4 cut(s) 547, 604, 617, 990
PflFI GACNNNGTC 1 cut(s) 1121
PflMI CCANNNNNTGG 1 cut(s) 935
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 3 cut(s) 518, 694, 916
PpsI GAGTC 3 cut(s) 518, 694, 916
Ppu21I YACGTR 1 cut(s) 976
PpuMI RGGWCCY 2 cut(s) 866, 1117
PshAI GACNNNNGTC 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 583
Psp5II RGGWCCY 2 cut(s) 866, 1117
Psp6I CCWGG 2 cut(s) 664, 1119
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 2 cut(s) 664, 1119
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 4 cut(s) 326, 662, 866, 1117
PspPPI RGGWCCY 2 cut(s) 866, 1117
PsyI GACNNNGTC 1 cut(s) 1121
RsaI GTAC 5 cut(s) 160, 345, 494, 632, 1019
RsaNI GTAC 5 cut(s) 159, 344, 493, 631, 1018
SacI GAGCTC 1 cut(s) 583
SaqAI TTAA 4 cut(s) 410, 498, 654, 678
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 4 cut(s) 220, 352, 727, 775
Sau96I GGNCC 4 cut(s) 326, 662, 866, 1117
ScaI AGTACT 1 cut(s) 160
SchI GAGTC 3 cut(s) 519, 694, 916
ScrFI CCNGG 4 cut(s) 324, 666, 821, 1121
SduI GDGCHC 1 cut(s) 583
SexAI ACCWGGT 1 cut(s) 1119
SfaNI GCATC 2 cut(s) 284, 1146
SinI GGWCC 4 cut(s) 326, 662, 866, 1117
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
Sse9I AATT 8 cut(s) 33, 114, 246, 269, 358, 382, 456, 790
SspI AATATT 1 cut(s) 598
SspMI CTAG 9 cut(s) 29, 288, 294, 300, 312, 330, 507, 951, 1136
SstI GAGCTC 1 cut(s) 583
StyD4I CCNGG 4 cut(s) 322, 664, 819, 1119
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 3 cut(s) 191, 490, 569
TaiI ACGT 3 cut(s) 805, 819, 978
TaqI TCGA 4 cut(s) 513, 615, 1044, 1125
TasI AATT 8 cut(s) 33, 114, 246, 269, 358, 382, 456, 790
TatI WGTACW 4 cut(s) 158, 343, 492, 630
TfiI GAWTC 4 cut(s) 547, 604, 617, 990
Tru1I TTAA 4 cut(s) 410, 498, 654, 678
Tru9I TTAA 4 cut(s) 410, 498, 654, 678
TscAI CASTG 5 cut(s) 156, 940, 949, 1011, 1074
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 3 cut(s) 107, 351, 1062
TspRI CASTG 5 cut(s) 156, 940, 949, 1011, 1074
Tth111I GACNNNGTC 1 cut(s) 1121
Van91I CCANNNNNTGG 1 cut(s) 935
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 4 cut(s) 326, 662, 866, 1117
XapI RAATTY 2 cut(s) 114, 246
XspI CTAG 9 cut(s) 29, 288, 294, 300, 312, 330, 507, 951, 1136
ZrmI AGTACT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.