FvH4_2g32291

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017074
Physical Location & Seq
Forward (+)
24517504 .. 24519421
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32291.t1

Sequence Viewer

Length: 1335 bp
ATGATATCACAGAAGATGGGAAGGAGGTCTATAATGCTTTTGCTAGCAATGCTGGGTTTGATTGTTATTAGTGGTAGTGAAGCAGTAGATGTAGATGTGAATGCTAAAGCTAAGAAACATTTTTTGGAAATTGGTAACAAGTTGAAGCTTCTCAATAGGCGTGGAGTGAAGAGTATTAAGAGTGAGGATGGAGACATTGTGGACTGTGTTGACATCTACAAACAGCCTGCTTTTGATCATCCAGCATTGCAAAACCACAAAATTCAGATGAGACCTAGTTTTCAATTTGCATCACAGGCTCCTAGTCCTAGTCCTACTCCCGGTCCGAGCAGGAGTCCGGTACAAAATGATCATTATTCATCTCAGCTTATCTCTCAATATTGGCAAAGGAGTGGAAGCTGTCCAGATGGAACCATTCCCATTAGAAGAATCCGAAGGCGGGACTTGCTAAGCGCTGCTTCACTTGAACGCTTTGGGATGAAAAACCCAGCACCAAATTCCTACTCAACCAATGATGATATAGGGACCGTGTACATTAACAAAACCAGAGTGGATCTTGGTCCTCAAATCAATCGTTCTGCTGCGATTCTTGTGACTACTGGATACAGTTACATAGGAGCTCAGGGAGATATAAATATTTGGAATCCAAAAGTCGAGTCACCGGATGAGTACACTACTGCTCAAATCTGGCTTAAGAATGGACCTGGAAATGCCTTTGAAAGTGTCGAGTCAGGCTGGATGGTAAACCCAAAGTTGTATGGTGATTCAGCTACTCGACTTTTCGTATATTGGACAGTGGACTCATACAAATCAACAGGATGCTTTGATCTAACTTGCTCAGGGTTTGTGCAAACTAACACAGACATAGCCCTTGGTATGCCCCTAGGACCCGTATCAAGTGAAGGAGGACCCCAATATCAAACCACTTTCAGCATCAACAAGGATCCAAACACAGGCAATTGGTGGCTACGTTTGGGTCAAAACGTCCCGGTAGGATACTGGCCGCCAAGTCTCTTCTCCTATATGAAACAAGGTCCTGCAACATTGGTTGAATGGGGTGGGGAGGTGTATAGCTCCAAGGTGAAGAAGACTCGTCCACACACTGCAACTGCAATGGGAAGTGGGAATTTTGCTTCAGGCTTAGATGGATATGCTTGTTACGTGAAACAAGTGAGAATTGTAGATTATTCAATGCAACTGAAGTACCCAGAATGGGTAGGCACTTTTGTAGATGAAGCATACTGCTACTCTGCACAGAACTATGCTCAGTCACTTGCTCAAGAACCTACATTTTACTTTGGAGGACCTGGTCGTTATTTTATGTATTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

445

Amino Acids

49.29

Weight (kDa)

8.74

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 58 - 169 9.8e-35 Neprosin activation peptide
Neprosin PF03080 222 - 436 6e-75 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 439, 1004
AclWI GGATC 3 cut(s) 561, 938, 951
AcoI YGGCCR 1 cut(s) 1001
AcsI RAATTY 3 cut(s) 261, 496, 1126
AcuI CTGAAG 2 cut(s) 1119, 1220
AfaI GTAC 4 cut(s) 342, 533, 671, 1205
AfeI AGCGCT 1 cut(s) 454
AfiI CCNNNNNNNGG 4 cut(s) 320, 439, 953, 1213
AflII CTTAAG 1 cut(s) 692
AgsI TTSAA 6 cut(s) 145, 284, 467, 719, 1052, 1191
AjnI CCWGG 2 cut(s) 703, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 7 cut(s) 110, 148, 367, 399, 620, 770, 1074
AluI AGCT 7 cut(s) 110, 148, 367, 399, 620, 770, 1074
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 3 cut(s) 186, 265, 1016
AlwI GGATC 3 cut(s) 561, 938, 951
Aor51HI AGCGCT 1 cut(s) 454
AoxI GGCC 1 cut(s) 1001
ApeKI GCWGC 2 cut(s) 455, 581
ApoI RAATTY 3 cut(s) 261, 496, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AspA2I CCTAGG 1 cut(s) 883
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
AsuC2I CCSGG 2 cut(s) 321, 989
AsuHPI GGTGA 3 cut(s) 651, 773, 1093
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
AvrII CCTAGG 1 cut(s) 883
BaeI ACNNNNGTAYC 2 cut(s) 1187, 1220
BamHI GGATCC 1 cut(s) 943
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 1094
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 442, 568
BccI CCATC 5 cut(s) 10, 182, 401, 733, 1139
BciT130I CCWGG 2 cut(s) 705, 1308
BciVI GTATCC 2 cut(s) 596, 989
BclI TGATCA 2 cut(s) 235, 349
BcnI CCSGG 2 cut(s) 321, 989
BcoDI GTCTC 3 cut(s) 186, 265, 1016
BfaI CTAG 5 cut(s) 44, 276, 303, 309, 884
BfoI RGCGCY 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 692
BfuI GTATCC 2 cut(s) 596, 989
BisI GCNGC 3 cut(s) 456, 582, 1004
BlnI CCTAGG 1 cut(s) 883
BlpI GCTNAGC 1 cut(s) 449
BlsI GCNGC 3 cut(s) 457, 583, 1005
Bme1390I CCNGG 4 cut(s) 321, 705, 989, 1308
Bme18I GGWCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
BmgT120I GGNCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
BmiI GGNNCC 6 cut(s) 300, 412, 526, 889, 910, 945
BmrFI CCNGG 4 cut(s) 321, 705, 989, 1308
BmsI GCATC 3 cut(s) 299, 809, 942
BmtI GCTAGC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 1094
Bpu10I CCTNAGC 2 cut(s) 621, 838
Bpu1102I GCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 1263
BpuMI CCSGG 2 cut(s) 321, 989
BsaAI YACGTR 1 cut(s) 1162
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 3 cut(s) 871, 883, 1077
BsaWI WCCGGW 2 cut(s) 337, 661
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 439, 953, 1213
Bse1I ACTGG 2 cut(s) 604, 1004
Bse3DI GCAATG 3 cut(s) 54, 245, 1119
BseBI CCWGG 2 cut(s) 705, 1308
BseDI CCNNGG 3 cut(s) 871, 883, 1077
BseGI GGATG 6 cut(s) 193, 238, 483, 670, 744, 824
BseLI CCNNNNNNNGG 4 cut(s) 320, 439, 953, 1213
BseMI GCAATG 3 cut(s) 54, 245, 1119
BseMII CTCAG 4 cut(s) 377, 635, 852, 1280
BseNI ACTGG 2 cut(s) 604, 1004
BseXI GCAGC 2 cut(s) 442, 568
BseYI CCCAGC 2 cut(s) 52, 487
BsgI GTGCAG 1 cut(s) 1236
BshFI GGCC 1 cut(s) 1003
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 4 cut(s) 321, 338, 662, 989
BslFI GGGAC 3 cut(s) 455, 538, 971
BslI CCNNNNNNNGG 4 cut(s) 320, 439, 953, 1213
BsmAI GTCTC 3 cut(s) 186, 265, 1016
BsmFI GGGAC 3 cut(s) 455, 538, 971
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 1 cut(s) 1003
Bso31I GGTCTC 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 5 cut(s) 235, 349, 553, 826, 943
Bsp1720I GCTNAGC 1 cut(s) 449
BspACI CCGC 2 cut(s) 439, 1004
BspANI GGCC 1 cut(s) 1003
BspCNI CTCAG 4 cut(s) 376, 634, 851, 1279
BspLI GGNNCC 6 cut(s) 300, 412, 526, 889, 910, 945
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 561, 938, 951
BspTI CTTAAG 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 3 cut(s) 54, 245, 1119
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 2 cut(s) 604, 1004
BssECI CCNNGG 3 cut(s) 871, 883, 1077
BssMI GATC 5 cut(s) 235, 349, 553, 826, 943
BssT1I CCWWGG 3 cut(s) 871, 883, 1077
Bst2UI CCWGG 2 cut(s) 705, 1308
Bst4CI ACNGT 4 cut(s) 206, 529, 608, 796
Bst6I CTCTTC 2 cut(s) 164, 1019
BstAFI CTTAAG 1 cut(s) 692
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 1162
BstC8I GCNNGC 2 cut(s) 45, 228
BstDEI CTNAG 7 cut(s) 111, 363, 449, 621, 838, 1141, 1266
BstF5I GGATG 6 cut(s) 193, 238, 483, 670, 744, 824
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 5 cut(s) 238, 352, 556, 829, 946
BstMAI GTCTC 3 cut(s) 186, 265, 1016
BstMBI GATC 5 cut(s) 235, 349, 553, 826, 943
BstMWI GCNNNNNNNGC 3 cut(s) 49, 296, 445
BstNI CCWGG 2 cut(s) 705, 1308
BstSCI CCNGG 4 cut(s) 319, 703, 987, 1306
BstV1I GCAGC 2 cut(s) 442, 568
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 2 cut(s) 553, 943
BstYI RGATCY 2 cut(s) 553, 943
BsuI GTATCC 2 cut(s) 596, 989
BsuRI GGCC 1 cut(s) 1003
BtsCI GGATG 6 cut(s) 193, 238, 483, 670, 744, 824
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 2 cut(s) 801, 1101
Cac8I GCNNGC 2 cut(s) 45, 228
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
CpoI CGGWCCG 1 cut(s) 323
CsiI ACCWGGT 1 cut(s) 1306
Csp6I GTAC 4 cut(s) 341, 532, 670, 1204
CspCI CAANNNNNGTGG 2 cut(s) 142, 177
CspI CGGWCCG 1 cut(s) 323
CviQI GTAC 4 cut(s) 341, 532, 670, 1204
DdeI CTNAG 7 cut(s) 111, 363, 449, 621, 838, 1141, 1266
DpnI GATC 5 cut(s) 237, 351, 555, 828, 945
DpnII GATC 5 cut(s) 235, 349, 553, 826, 943
EaeI YGGCCR 1 cut(s) 1001
Eam1104I CTCTTC 2 cut(s) 164, 1019
EarI CTCTTC 2 cut(s) 164, 1019
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 3 cut(s) 871, 883, 1077
Eco24I GRGCYC 1 cut(s) 622
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
Eco47III AGCGCT 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 2 cut(s) 1119, 1220
EcoICRI GAGCTC 1 cut(s) 620
EcoO109I RGGNCCY 4 cut(s) 887, 908, 1034, 1304
EcoRII CCWGG 2 cut(s) 703, 1306
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 871, 883, 1077
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 3 cut(s) 871, 883, 1077
FalI AAGNNNNNCTT 2 cut(s) 442, 474
FaqI GGGAC 3 cut(s) 455, 538, 971
FauI CCCGC 1 cut(s) 432
FbaI TGATCA 2 cut(s) 235, 349
Fnu4HI GCNGC 3 cut(s) 456, 582, 1004
FokI GGATG 6 cut(s) 200, 225, 490, 677, 751, 831
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 3 cut(s) 456, 582, 1004
FspBI CTAG 5 cut(s) 44, 276, 303, 309, 884
GlaI GCGC 1 cut(s) 454
GluI GCNGC 3 cut(s) 456, 582, 1004
GsaI CCCAGC 2 cut(s) 56, 491
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 1 cut(s) 1003
HapII CCGG 4 cut(s) 321, 338, 662, 989
HhaI GCGC 1 cut(s) 455
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 9 cut(s) 334, 429, 586, 643, 656, 728, 764, 800, 1090
HpaII CCGG 4 cut(s) 321, 338, 662, 989
HphI GGTGA 3 cut(s) 651, 773, 1093
Hpy166II GTNNAC 7 cut(s) 202, 211, 532, 672, 745, 799, 1097
Hpy188I TCNGA 3 cut(s) 267, 327, 434
Hpy188III TCNNGA 2 cut(s) 404, 1280
Hpy8I GTNNAC 7 cut(s) 202, 211, 532, 672, 745, 799, 1097
HpyAV CCTTC 3 cut(s) 15, 429, 896
HpyCH4III ACNGT 4 cut(s) 206, 529, 608, 796
HpyCH4IV ACGT 3 cut(s) 970, 984, 1161
HpyCH4V TGCA 8 cut(s) 250, 290, 850, 1040, 1106, 1112, 1195, 1253
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 296, 445
HpyF3I CTNAG 7 cut(s) 111, 363, 449, 621, 838, 1141, 1266
HpySE526I ACGT 3 cut(s) 970, 984, 1161
HspAI GCGC 1 cut(s) 453
Ksp22I TGATCA 2 cut(s) 235, 349
Kzo9I GATC 5 cut(s) 235, 349, 553, 826, 943
LmnI GCTCC 3 cut(s) 304, 617, 1079
Lsp1109I GCAGC 2 cut(s) 442, 568
LweI GCATC 3 cut(s) 299, 809, 942
MabI ACCWGGT 1 cut(s) 1306
MaeI CTAG 5 cut(s) 44, 276, 303, 309, 884
MaeII ACGT 3 cut(s) 970, 984, 1161
MaeIII GTNAC 6 cut(s) 134, 592, 608, 657, 1157, 1269
MalI GATC 5 cut(s) 237, 351, 555, 828, 945
MboI GATC 5 cut(s) 235, 349, 553, 826, 943
MboII GAAGA 6 cut(s) 25, 181, 438, 1006, 1096, 1099
MfeI CAATTG 1 cut(s) 958
MflI RGATCY 2 cut(s) 553, 943
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 7 cut(s) 129, 261, 284, 496, 958, 1126, 1176
MlyI GAGTC 5 cut(s) 343, 665, 737, 794, 1084
MnlI CCTC 6 cut(s) 18, 178, 573, 899, 1057, 1295
MseI TTAA 3 cut(s) 177, 537, 693
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 4 cut(s) 321, 338, 662, 989
MspR9I CCNGG 4 cut(s) 321, 705, 989, 1308
MunI CAATTG 1 cut(s) 958
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 2 cut(s) 705, 1308
MwoI GCNNNNNNNGC 3 cut(s) 49, 296, 445
NciI CCSGG 2 cut(s) 321, 989
NdeII GATC 5 cut(s) 235, 349, 553, 826, 943
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 6 cut(s) 300, 412, 526, 889, 910, 945
NmuCI GTSAC 3 cut(s) 592, 657, 1269
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 4 cut(s) 429, 586, 643, 764
PflFI GACNNNGTC 1 cut(s) 1308
PkrI GCNGC 3 cut(s) 457, 583, 1005
PleI GAGTC 5 cut(s) 342, 664, 736, 794, 1084
PpsI GAGTC 5 cut(s) 342, 664, 736, 794, 1084
Ppu21I YACGTR 1 cut(s) 1162
PpuMI RGGWCCY 4 cut(s) 887, 908, 1034, 1304
Psp124BI GAGCTC 1 cut(s) 622
Psp5II RGGWCCY 4 cut(s) 887, 908, 1034, 1304
Psp6I CCWGG 2 cut(s) 703, 1306
PspFI CCCAGC 2 cut(s) 52, 487
PspGI CCWGG 2 cut(s) 703, 1306
PspN4I GGNNCC 6 cut(s) 300, 412, 526, 889, 910, 945
PspPI GGNCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
PspPPI RGGWCCY 4 cut(s) 887, 908, 1034, 1304
PsuI RGATCY 2 cut(s) 553, 943
PsyI GACNNNGTC 1 cut(s) 1308
RsaI GTAC 4 cut(s) 342, 533, 671, 1205
RsaNI GTAC 4 cut(s) 341, 532, 670, 1204
Rsr2I CGGWCCG 1 cut(s) 323
RsrII CGGWCCG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 3 cut(s) 177, 537, 693
SatI GCNGC 3 cut(s) 456, 582, 1004
Sau3AI GATC 5 cut(s) 235, 349, 553, 826, 943
Sau96I GGNCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
SchI GAGTC 5 cut(s) 343, 665, 737, 794, 1084
ScrFI CCNGG 4 cut(s) 321, 705, 989, 1308
SduI GDGCHC 1 cut(s) 622
SexAI ACCWGGT 1 cut(s) 1306
SfaNI GCATC 3 cut(s) 299, 809, 942
SinI GGWCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
SmlI CTYRAG 2 cut(s) 692, 1278
SmoI CTYRAG 2 cut(s) 692, 1278
Sse9I AATT 7 cut(s) 129, 261, 284, 496, 958, 1126, 1176
SsiI CCGC 2 cut(s) 439, 1004
SspI AATATT 2 cut(s) 380, 637
SspMI CTAG 5 cut(s) 44, 276, 303, 309, 884
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 4 cut(s) 319, 703, 987, 1306
StyI CCWWGG 3 cut(s) 871, 883, 1077
TaaI ACNGT 4 cut(s) 206, 529, 608, 796
TaiI ACGT 3 cut(s) 973, 987, 1164
TaqI TCGA 3 cut(s) 654, 726, 775
TasI AATT 7 cut(s) 129, 261, 284, 496, 958, 1126, 1176
TatI WGTACW 2 cut(s) 531, 669
TauI GCSGC 1 cut(s) 1006
TfiI GAWTC 4 cut(s) 429, 586, 643, 764
Tru1I TTAA 3 cut(s) 177, 537, 693
Tru9I TTAA 3 cut(s) 177, 537, 693
TscAI CASTG 2 cut(s) 801, 1108
TseFI GTSAC 3 cut(s) 592, 657, 1269
TseI GCWGC 2 cut(s) 455, 581
Tsp45I GTSAC 3 cut(s) 592, 657, 1269
TspDTI ATGAA 4 cut(s) 348, 494, 1040, 1248
TspRI CASTG 2 cut(s) 801, 1108
Tth111I GACNNNGTC 1 cut(s) 1308
Vha464I CTTAAG 1 cut(s) 692
VpaK11BI GGWCC 8 cut(s) 323, 525, 560, 701, 887, 908, 1034, 1304
XapI RAATTY 3 cut(s) 261, 496, 1126
XmaJI CCTAGG 1 cut(s) 883
XspI CTAG 5 cut(s) 44, 276, 303, 309, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.